Difference between revisions of "Team:SHSBNU China/Measurement"

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    <title>SHSBNU-China iGEM 2017 Home Page</title>
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Measurement</h1>
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            <a href="https://2017.igem.org/Team:SHSBNU_China"> <img src="https://static.igem.org/mediawiki/2017/a/aa/SHSBNU_17_Banner.jpg" /></a>
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<p>There are a lot of exciting parts in the Registry, but many parts have still not been characterized. Synthetic Biology needs great measurement approaches for characterizing new parts, and efficient new methods for characterizing many parts at once. If you've done something exciting in the area of Measurement, describe it here!</p>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Project" style = "opacity: 1;">Project</a>
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            <a> | </a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Model" style = "opacity: 1;">Model</a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/HP" style = "opacity: 1;">HP</a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Safety" style = "opacity: 1;">Safety</a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Parts" style = "opacity: 1;">Parts</a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Team" style = "opacity: 1;">Team</a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Achievement" style = "opacity: 1;">Achievement</a>
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<h3>Best Innovation in Measurement Special Prize</h3>
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<p>If you've done excellent work in measurement, you should consider nominating your team for this special prize. Designing great measurement approaches for characterizing new parts or developing and implementing an efficient new method for characterizing thousands of parts are good examples.
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            <p style = "font-family:arial;color:#444;font-size:30px; text-align: center">Model</p>
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To compete for the <a href="https://2017.igem.org/Judging/Awards">Best Innovation in Measurement prize</a>, please describe your work on this page and also fill out the description on the <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a>.
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You must also delete the message box on the top of this page to be eligible for this prize.
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Model" style = "opacity: 1;">Model</a>
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            <a> | </a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Measurement" style = "opacity: 1;">Measurement</a>
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<h5>Inspiration</h5>
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<p>You can look at what other teams did to get some inspiration! <br />
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Here are a few examples:</p>
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                <p style = "font-family:arial;color:#444444;font-size:32px; text-align: center"> Measurement </p>
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                <p>First, we used logarithmic phase (log phase) bacteria to measure their fluorescence by using flow cytometry. Log phase bacteria are in the best phase of growing so that its data outcome would be the most accurate and valuable. After transforming the bacteria into LB medium and incubate overnight (for 8-12 hours) at 37°C, the medium is diluted 196-fold and incubated for 3 hours; then the bacteria is diluted 700-fold with fresh M9+Glycerol medium or sodium thiosulfate to reach log phase, then we use flow cytometry to measure the fluorescent intensity. </p>
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<li><a href="https://2016.igem.org/Team:Stanford-Brown">2016 Stanford-Brown</a></li>
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                <img src="https://static.igem.org/mediawiki/2017/f/f6/SHSBNU_17_21a00.jpg"/>
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<li><a href="https://2016.igem.org/Team:Genspace">2016 Genspace</a></li>
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                <img src="https://static.igem.org/mediawiki/2017/5/58/SHSBNU_17_21a01.jpg" style="height:300px"/>
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<li><a href="https://2015.igem.org/Team:William_and_Mary">2015 William and Mary</a></li>
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                <br/>
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<li><a href="https://2014.igem.org/Team:Aachen">2014 Aachen  </a></li>
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                <img src="https://static.igem.org/mediawiki/2017/f/fd/SHSBNU_17_21a02.jpg" style="height:300px"/>
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                <p>Second, in order to examine the effect of mixing the outcome color of our bacteria with human excrement, we used curry to simulate the excrement, with different color depth simulating different kinds of excrements. This is much more safe and friendly because we no longer need to get in touch with excrement. </p>
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                <img src="https://static.igem.org/mediawiki/2017/7/71/SHSBNU_17_13c02.JPG"/>
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                <img src="https://static.igem.org/mediawiki/2017/b/b3/SHSBNU_17_20b00.jpg" style="height:400px/>
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                <p>Thirdly, in order to test how would our bacteria perform in anaerobic environment, we spread oil on the medium after spreading the bacteria, using oil to isolate oxygen and the bacteria. </p>
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                <img src="https://static.igem.org/mediawiki/2017/2/23/SHSBNU_17_21a03.jpg" style="height:300px"/>
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                <p>Under anaerobic condition, GFP is difficult to be maturated. So We measure sfGFP fluorescent after 1h maturation. </p>
  
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            <a href="http://www.shsbnu.net" style = "float:left"><img src = "https://static.igem.org/mediawiki/2017/4/44/SHSBNU_Logo_256X256.jpg" style="height:48px; width:48px; " border=0></a>
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            <a href="http://www.azirquantum.net.cn" style = "float:left"><img src = "https://static.igem.org/mediawiki/2017/1/1f/Azir_Logo_256X256.jpg" style="height:48px; width:48px; " border=0></a>
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            <a href="http://www.bluepha.com" style = "float:right"><img src = "https://static.igem.org/mediawiki/2017/b/bc/Bluepha_Logo_256X256.jpg" style="height:48px; width:48px; " border=0></a>
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            <P style = "font-family:arial;color:#5C5C5C;font-size:10px; text-align: center">iGEM 2017</P>
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            <P style = "font-family:arial;color:#5C5C5C;font-size:10px; text-align: center">Made by Azir.</P>
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            <p style = "font-family:arial;color:#5C5C5C;font-size:10px; text-align: center">The Second High School Attached Beijing Normal University International Division </p>
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Latest revision as of 18:31, 1 November 2017

SHSBNU-China iGEM 2017 Home Page

Measurement

First, we used logarithmic phase (log phase) bacteria to measure their fluorescence by using flow cytometry. Log phase bacteria are in the best phase of growing so that its data outcome would be the most accurate and valuable. After transforming the bacteria into LB medium and incubate overnight (for 8-12 hours) at 37°C, the medium is diluted 196-fold and incubated for 3 hours; then the bacteria is diluted 700-fold with fresh M9+Glycerol medium or sodium thiosulfate to reach log phase, then we use flow cytometry to measure the fluorescent intensity.


Second, in order to examine the effect of mixing the outcome color of our bacteria with human excrement, we used curry to simulate the excrement, with different color depth simulating different kinds of excrements. This is much more safe and friendly because we no longer need to get in touch with excrement.

Under anaerobic condition, GFP is difficult to be maturated. So We measure sfGFP fluorescent after 1h maturation.

iGEM 2017

Made by Azir.

The Second High School Attached Beijing Normal University International Division