Difference between revisions of "Team:SHSBNU China/Measurement"

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                <p style = "font-family:arial;color:#444444;font-size:32px; text-align: center"> Measurement </p>
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                <p>First, we used logarithmic phase (log phase) bacteria to measure their fluorescence by using flow cytometry. Log phase bacteria are in the best phase of growing so that its data outcome would be the most accurate and valuable. After transforming the bacteria into LB medium and incubate overnight (for 8-12 hours) at 37°C, the medium is diluted 196-fold and incubated for 3 hours; then the bacteria is diluted 700-fold with fresh M9+Glycerol medium or sodium thiosulfate to reach log phase, then we use flow cytometry to measure the fluorescent intensity. </p>
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                <p>Second, in order to examine the effect of mixing the outcome color of our bacteria with human excrement, we used curry to simulate the excrement, with different color depth simulating different kinds of excrements. This is much more safe and friendly because we no longer need to get in touch with excrement. </p>
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                <p>Thirdly, in order to test how would our bacteria perform in anaerobic environment, we spread oil on the medium after spreading the bacteria, using oil to isolate oxygen and the bacteria. </p>
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                <p>Under anaerobic condition, GFP is difficult to be maturated. So We measure sfGFP fluorescent after 1h maturation. </p>
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            <P style = "font-family:arial;color:#5C5C5C;font-size:10px; text-align: center">iGEM 2017</P>
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            <P style = "font-family:arial;color:#5C5C5C;font-size:10px; text-align: center">Made by Azir.</P>
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            <p style = "font-family:arial;color:#5C5C5C;font-size:10px; text-align: center">The Second High School Attached Beijing Normal University International Division </p>
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Latest revision as of 18:31, 1 November 2017

SHSBNU-China iGEM 2017 Home Page

Measurement

First, we used logarithmic phase (log phase) bacteria to measure their fluorescence by using flow cytometry. Log phase bacteria are in the best phase of growing so that its data outcome would be the most accurate and valuable. After transforming the bacteria into LB medium and incubate overnight (for 8-12 hours) at 37°C, the medium is diluted 196-fold and incubated for 3 hours; then the bacteria is diluted 700-fold with fresh M9+Glycerol medium or sodium thiosulfate to reach log phase, then we use flow cytometry to measure the fluorescent intensity.


Second, in order to examine the effect of mixing the outcome color of our bacteria with human excrement, we used curry to simulate the excrement, with different color depth simulating different kinds of excrements. This is much more safe and friendly because we no longer need to get in touch with excrement.

Under anaerobic condition, GFP is difficult to be maturated. So We measure sfGFP fluorescent after 1h maturation.

iGEM 2017

Made by Azir.

The Second High School Attached Beijing Normal University International Division