|
|
| (One intermediate revision by the same user not shown) |
| Line 82: |
Line 82: |
| | <p>First, we used logarithmic phase (log phase) bacteria to measure their fluorescence by using flow cytometry. Log phase bacteria are in the best phase of growing so that its data outcome would be the most accurate and valuable. After transforming the bacteria into LB medium and incubate overnight (for 8-12 hours) at 37°C, the medium is diluted 196-fold and incubated for 3 hours; then the bacteria is diluted 700-fold with fresh M9+Glycerol medium or sodium thiosulfate to reach log phase, then we use flow cytometry to measure the fluorescent intensity. </p> | | <p>First, we used logarithmic phase (log phase) bacteria to measure their fluorescence by using flow cytometry. Log phase bacteria are in the best phase of growing so that its data outcome would be the most accurate and valuable. After transforming the bacteria into LB medium and incubate overnight (for 8-12 hours) at 37°C, the medium is diluted 196-fold and incubated for 3 hours; then the bacteria is diluted 700-fold with fresh M9+Glycerol medium or sodium thiosulfate to reach log phase, then we use flow cytometry to measure the fluorescent intensity. </p> |
| | <img src="https://static.igem.org/mediawiki/2017/f/f6/SHSBNU_17_21a00.jpg"/> | | <img src="https://static.igem.org/mediawiki/2017/f/f6/SHSBNU_17_21a00.jpg"/> |
| − | <img src="https://static.igem.org/mediawiki/2017/5/58/SHSBNU_17_21a01.jpg" style="height:300"/> | + | <img src="https://static.igem.org/mediawiki/2017/5/58/SHSBNU_17_21a01.jpg" style="height:300px"/> |
| − | <img src="https://static.igem.org/mediawiki/2017/f/fd/SHSBNU_17_21a02.jpg" style="height:300"/> | + | <br/> |
| | + | <img src="https://static.igem.org/mediawiki/2017/f/fd/SHSBNU_17_21a02.jpg" style="height:300px"/> |
| | <p>Second, in order to examine the effect of mixing the outcome color of our bacteria with human excrement, we used curry to simulate the excrement, with different color depth simulating different kinds of excrements. This is much more safe and friendly because we no longer need to get in touch with excrement. </p> | | <p>Second, in order to examine the effect of mixing the outcome color of our bacteria with human excrement, we used curry to simulate the excrement, with different color depth simulating different kinds of excrements. This is much more safe and friendly because we no longer need to get in touch with excrement. </p> |
| | <img src="https://static.igem.org/mediawiki/2017/7/71/SHSBNU_17_13c02.JPG"/> | | <img src="https://static.igem.org/mediawiki/2017/7/71/SHSBNU_17_13c02.JPG"/> |
| − | <img src="https://static.igem.org/mediawiki/2017/b/b3/SHSBNU_17_20b00.jpg"/> | + | <img src="https://static.igem.org/mediawiki/2017/b/b3/SHSBNU_17_20b00.jpg" style="height:400px/> |
| | <p>Thirdly, in order to test how would our bacteria perform in anaerobic environment, we spread oil on the medium after spreading the bacteria, using oil to isolate oxygen and the bacteria. </p> | | <p>Thirdly, in order to test how would our bacteria perform in anaerobic environment, we spread oil on the medium after spreading the bacteria, using oil to isolate oxygen and the bacteria. </p> |
| − | <img src="https://static.igem.org/mediawiki/2017/2/23/SHSBNU_17_21a03.jpg"/> | + | <img src="https://static.igem.org/mediawiki/2017/2/23/SHSBNU_17_21a03.jpg" style="height:300px"/> |
| | <p>Under anaerobic condition, GFP is difficult to be maturated. So We measure sfGFP fluorescent after 1h maturation. </p> | | <p>Under anaerobic condition, GFP is difficult to be maturated. So We measure sfGFP fluorescent after 1h maturation. </p> |
| | | | |
