Difference between revisions of "Team:WashU StLouis/Results"

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<h1>Results</h1>
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<p>Here you can describe the results of your project and your future plans. </p>
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    <p style="font-size: 2.5vw; text-align:center; padding 0.5vw">Dsup</p>
  
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<h5>What should this page contain?</h5>
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    <p style="font-size:1.5vw">The cells that were transformed to contain Dsup were found to have significantly higher survival rates than those without Dsup. Overall, cells containing Dsup--both induced and uninduced--showed an average survival rate of approximately 26% after four hours of exposure to UV-B radiation, while the cells containing only blue chromoprotein (without any radiation resistance genes) showed a survival rate of 0% after four hours. The most pronounced difference between cells containing Dsup and cells containing no radiation resistance was found at three hours, with Dsup-containing cells having an average survival rate of 49% and non-Dsup-containing cells having an average survival rate of 2.3%, meaning that cells with Dsup survived at a rate that was, on average, 2130% higher than the rate at which non-Dsup cells survived.
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<li> Clearly and objectively describe the results of your work.</li>
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<h5>You should also describe what your results mean: </h5>
 
  
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    <p style="font-size:1.5vw">There was no significant difference observed in the survival rates of cells in which expression of Dsup was induced via addition of IPTG and cells in which it was not induced, indicating extremely leaky expression in the Lac promoter. </p>
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
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<h5> Project Achievements </h5>
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    <p style="font-size: 2.5vw; text-align:center; padding 0.5vw">Failed Expression and mRNA Secondary Structures</p>
  
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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    <p style="font-size:1.5vw">The failure of uvsE, phrAc, and phrAt to provide any radiation resistance can likely be attributed to the strong secondary structures present in the radiation resistance portions of these constructs and minimal secondary structures present in the BCP portion of the constructs. This is consistent with the observation that blue colonies were present on the plates on which these cells were grown, but no radiation resistance was observed.</p>
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<li>A list of linked bullet points of the successful results during your project</li>
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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<h5>Inspiration</h5>
 
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<p>See how other teams presented their results.</p>
 
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
 
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
 
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
 
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Revision as of 19:08, 1 November 2017

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Dsup

The cells that were transformed to contain Dsup were found to have significantly higher survival rates than those without Dsup. Overall, cells containing Dsup--both induced and uninduced--showed an average survival rate of approximately 26% after four hours of exposure to UV-B radiation, while the cells containing only blue chromoprotein (without any radiation resistance genes) showed a survival rate of 0% after four hours. The most pronounced difference between cells containing Dsup and cells containing no radiation resistance was found at three hours, with Dsup-containing cells having an average survival rate of 49% and non-Dsup-containing cells having an average survival rate of 2.3%, meaning that cells with Dsup survived at a rate that was, on average, 2130% higher than the rate at which non-Dsup cells survived.

There was no significant difference observed in the survival rates of cells in which expression of Dsup was induced via addition of IPTG and cells in which it was not induced, indicating extremely leaky expression in the Lac promoter.

Failed Expression and mRNA Secondary Structures

The failure of uvsE, phrAc, and phrAt to provide any radiation resistance can likely be attributed to the strong secondary structures present in the radiation resistance portions of these constructs and minimal secondary structures present in the BCP portion of the constructs. This is consistent with the observation that blue colonies were present on the plates on which these cells were grown, but no radiation resistance was observed.