Difference between revisions of "Team:SHSBNU China/Notebook"

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{{SHSBNU_China}}
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    <title>SHSBNU-China iGEM 2017 Home Page</title>
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<h1>Notebook</h1>
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    <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:SHSBNU_China/CSS1?action=raw&ctype=text/css" />
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<p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p>
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<h5>What should this page have?</h5>
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    <div style="overflow: hidden; z-index: -1; position: fixed; height: 100%; width: 100%; top: 0px; left: 0px; ">
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<ul>
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        <img  id="background_picture" src="https://static.igem.org/mediawiki/2017/e/ee/SHSBNU_17_background.jpg" style="position: fixed; display: block; left: 0px; top: 0px;">
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<li>Chronological notes of what your team is doing.</li>
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    </div>
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<li> Brief descriptions of daily important events.</li>
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<li>Pictures of your progress. </li>
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<li>Mention who participated in what task.</li>
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</ul>
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</div>
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    <div id="wrap">
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        <div id="branding">
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            <a href="https://2017.igem.org/Team:SHSBNU_China"> <img src="https://static.igem.org/mediawiki/2017/a/aa/SHSBNU_17_Banner.jpg" /></a>
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        </div>
  
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<div class="column half_size">
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        <div id="nav">
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<h5>Inspiration</h5>
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        <nav class = "main" style = "font-size:16px; text-align: center;" >
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<p>You can see what others teams have done to organize their notes:</p>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Project" style = "opacity: 1;">Project</a>
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            <a> | </a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Model" style = "opacity: 1;">Model</a>
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            <a> | </a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/HP" style = "opacity: 1;">HP</a>
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            <a> | </a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Safety" style = "opacity: 1;">Safety</a>
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            <a> | </a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Parts" style = "opacity: 1;">Parts</a>
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            <a> | </a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Team" style = "opacity: 1;">Team</a>
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            <a> | </a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Achievement" style = "opacity: 1;">Achievement</a>
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        </nav>
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        </div>
  
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<ul>
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        <div id = "nav">
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<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
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        <nav class = "title" style = "font-size:20px; text-align: center">
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<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
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            <p style = "font-family:arial;color:#444;font-size:30px; text-align: center">Achievement</p>
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<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
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        </nav>
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<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
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        </div>
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</ul>
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</div>
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        <div id="nav">
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        <nav class = "cate" style = "font-size:16px; text-align: center;" >
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Achievement" style = "opacity: 1;">Achievement</a>
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            <a> | </a>
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            <a href="https://2017.igem.org/Team:SHSBNU_China/Notebook" style = "opacity: 1;">Notebook</a>
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        </nav>
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        </div>
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        <div class="post-75 post type-post status-publish format-standard hentry category-azir-quantum-science concavebox">
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            <div class="entry" style="opacity: 0.95;">
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                <p style = "font-family:arial;color:#444444;font-size:32px; text-align: center"> Notebook</p>
 +
                <p>SHSBNU_China iGEM2017 would like to share with you the document of the work done every week for our project. Although we have only 6 team members in the team, we go all out to do the experiments.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week1</p>
 +
                <p><strong>10/07/2017-16/07/2017</strong></p>
 +
                <p>Do Interlab experiment at Peking iGEM team lab.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week2</p>
 +
                <p><strong>17/07/2017-23/07/2017</strong></p>
 +
                <p>Transform chromoproteins BBa_K1033910, BBa_K1033916, BBa_K592010, into a pSEVA321 backbone(Provided by Bluepha).</p>
 +
                <p>Order Sequence synthesis of ThsS,ThsR,TtrS,TtrR by synbio, Suzhou.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week3</p>
 +
                <p><strong>24/07/2017-30/07/2017</strong></p>
 +
                <p>Transform chromoproteins BBa_K1033910, BBa_K1033916, BBa_K592010, into a pSEVA321 backbone(Provided by Bluepha).</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week4</p>
 +
                <p><strong>31/07/2017-06/08/2017</strong></p>
 +
                <p>Order Primers for constructing BBa_K2507004, BBa_K2507006, BBa_K2507008& BBa_K2507013 parts by Golden Gate.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week5</p>
 +
                <p><strong>07/08/2017-13/08/2017</strong></p>
 +
                <p>Receive synthesized sequences from synbio, and started PCR and then Golden Gate.</p>
 +
                <p>Failed several times of constructing the parts.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week6</p>
 +
                <p><strong>14/08/2017-20/08/2017</strong></p>
 +
                <p>Succeed construct BBa_K2507004, BBa_K2507006, BBa_K2507008& BBa_K2507013.</p>
 +
                <p>Order primers for constructing BBa_K2507009, BBa_K2507010, BBa_K2507011, BBa_K2507012& BBa_K25070014, BBa_K2507015, BBa_K2507016, BBa_K2507019.</p>
 +
                <p>Get E.coli Nissle 1917 from BNDS_China.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week7</p>
 +
                <p><strong>21/08/2017-27/08/2017</strong></p>
 +
                <p>Construct BBa_K2507009, BBa_K2507010, BBa_K2507011, BBa_K2507012& BBa_K25070014, BBa_K2507015, BBa_K2507016, BBa_K2507019.</p>
 +
                <p>Test which chromoprotein can be obvious in curry (replace excrement) for the first time.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week8</p>
 +
                <p><strong>28/08/2017-03/09/2017</strong></p>
 +
                <p>Qualitative test the response curve of ThsSR-gfp/ TtrSR-gfp.</p>
 +
                <p>Qualitative test the response curve of ThsSR-pink, purple, blue/ TtrSR-pink, purple, blue.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week9</p>
 +
                <p><strong>04/09/2017-10/09/2017</strong></p>
 +
                <p>Flow Cytometry test the response curve of ThsSR-gfp/TtrSR-gfp for the first time.</p>
 +
                <p>Qualitative test the response curve of ThsSR-gfp/ TtrSR-gfp in aerobic and anaerobic condition.</p>
 +
                <p>Go to the clinical laboratory of navy general hospital and made an interview</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week10</p>
 +
                <p><strong>11/09/2017-17/09/2017</strong></p>
 +
                <p>Flow Cytometry test the response curve of ThsSR-gfp/TtrSR-gfp for the second time.</p>
 +
                <p>Construct Biobrick BBa_K2507005, BBa_K2507007.</p>
 +
                <p>Construct Biobrick BBa_K2507012, BBa_K25070017.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week11</p>
 +
                <p><strong>18/09/2017-24/09/2017</strong></p>
 +
                <p>Transform Biobrick BBa_K2507004 with BBa_K2507012, and BBa_K2507006 with BBa_K2507017 into E. Coli Top10.</p>
 +
                <p>Test ThsSR-vio/TtrSR-vio system.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week12</p>
 +
                <p><strong>25/09/2017-01/10/2017</strong></p>
 +
                <p>Construct Biobrick BBa_K2507000-004.</p>
 +
                <p>Establish synthetic biology club in SHSBNU.</p>
 +
                <p>Update safety form.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week13</p>
 +
                <p><strong>02/10/2017-08/10/2017</strong></p>
 +
                <p>Start test chemical transform of <i>Bacillus subtilis.</i></p>
 +
                <p>Design Safety devices.
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week14</p>
 +
                <p><strong>09/10/2017-15/10/2017</strong></p>
 +
                <p>Flow Cytometry test the response curve of ThsSR-gfp/TtrSR-gfp for the third time.</p>
 +
                <p>Model our project.</p>
 +
 
 +
                <p style = "font-family:arial;color:#000000;font-size:20px; text-align: left"> Week15</p>
 +
                <p><strong>16/10/2017-22/10/2017</strong></p>
 +
                <p>Send part to Genscript.</p>
 +
            </div><!-- .entry -->
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            <div class="entry-footer" align = "center">
 +
                <a href="http://www.shsbnu.net" style = "float:left"><img src = "https://static.igem.org/mediawiki/2017/4/44/SHSBNU_Logo_256X256.jpg" style="height:48px; width:48px; " border=0></a>
 +
                <a href="http://www.azirquantum.net.cn" style = "float:left"><img src = "https://static.igem.org/mediawiki/2017/1/1f/Azir_Logo_256X256.jpg" style="height:48px; width:48px; " border=0></a>
 +
                <a href="https://2017.igem.org" style = "float:right"><img src = "https://static.igem.org/mediawiki/2017/6/60/IGEM_Logo_256X256.jpg" style="height:48px; width:48px; " border=0></a>
 +
                <a href="http://www.bluepha.com" style = "float:right"><img src = "https://static.igem.org/mediawiki/2017/b/bc/Bluepha_Logo_256X256.jpg" style="height:48px; width:48px; " border=0></a>
 +
                <P style = "font-family:arial;color:#5C5C5C;font-size:10px; text-align: center">iGEM 2017</P>
 +
                <P style = "font-family:arial;color:#5C5C5C;font-size:10px; text-align: center">Made by Azir.</P>
 +
                <p style = "font-family:arial;color:#5C5C5C;font-size:10px; text-align: center">The Second High School Attached Beijing Normal University International Division </p>
 +
            </div>
 +
 
 +
    </div><!-- .concavebox -->
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</body>
 
</html>
 
</html>

Latest revision as of 19:22, 1 November 2017

SHSBNU-China iGEM 2017 Home Page

Notebook

SHSBNU_China iGEM2017 would like to share with you the document of the work done every week for our project. Although we have only 6 team members in the team, we go all out to do the experiments.

Week1

10/07/2017-16/07/2017

Do Interlab experiment at Peking iGEM team lab.

Week2

17/07/2017-23/07/2017

Transform chromoproteins BBa_K1033910, BBa_K1033916, BBa_K592010, into a pSEVA321 backbone(Provided by Bluepha).

Order Sequence synthesis of ThsS,ThsR,TtrS,TtrR by synbio, Suzhou.

Week3

24/07/2017-30/07/2017

Transform chromoproteins BBa_K1033910, BBa_K1033916, BBa_K592010, into a pSEVA321 backbone(Provided by Bluepha).

Week4

31/07/2017-06/08/2017

Order Primers for constructing BBa_K2507004, BBa_K2507006, BBa_K2507008& BBa_K2507013 parts by Golden Gate.

Week5

07/08/2017-13/08/2017

Receive synthesized sequences from synbio, and started PCR and then Golden Gate.

Failed several times of constructing the parts.

Week6

14/08/2017-20/08/2017

Succeed construct BBa_K2507004, BBa_K2507006, BBa_K2507008& BBa_K2507013.

Order primers for constructing BBa_K2507009, BBa_K2507010, BBa_K2507011, BBa_K2507012& BBa_K25070014, BBa_K2507015, BBa_K2507016, BBa_K2507019.

Get E.coli Nissle 1917 from BNDS_China.

Week7

21/08/2017-27/08/2017

Construct BBa_K2507009, BBa_K2507010, BBa_K2507011, BBa_K2507012& BBa_K25070014, BBa_K2507015, BBa_K2507016, BBa_K2507019.

Test which chromoprotein can be obvious in curry (replace excrement) for the first time.

Week8

28/08/2017-03/09/2017

Qualitative test the response curve of ThsSR-gfp/ TtrSR-gfp.

Qualitative test the response curve of ThsSR-pink, purple, blue/ TtrSR-pink, purple, blue.

Week9

04/09/2017-10/09/2017

Flow Cytometry test the response curve of ThsSR-gfp/TtrSR-gfp for the first time.

Qualitative test the response curve of ThsSR-gfp/ TtrSR-gfp in aerobic and anaerobic condition.

Go to the clinical laboratory of navy general hospital and made an interview

Week10

11/09/2017-17/09/2017

Flow Cytometry test the response curve of ThsSR-gfp/TtrSR-gfp for the second time.

Construct Biobrick BBa_K2507005, BBa_K2507007.

Construct Biobrick BBa_K2507012, BBa_K25070017.

Week11

18/09/2017-24/09/2017

Transform Biobrick BBa_K2507004 with BBa_K2507012, and BBa_K2507006 with BBa_K2507017 into E. Coli Top10.

Test ThsSR-vio/TtrSR-vio system.

Week12

25/09/2017-01/10/2017

Construct Biobrick BBa_K2507000-004.

Establish synthetic biology club in SHSBNU.

Update safety form.

Week13

02/10/2017-08/10/2017

Start test chemical transform of Bacillus subtilis.

Design Safety devices.

Week14

09/10/2017-15/10/2017

Flow Cytometry test the response curve of ThsSR-gfp/TtrSR-gfp for the third time.

Model our project.

Week15

16/10/2017-22/10/2017

Send part to Genscript.

iGEM 2017

Made by Azir.

The Second High School Attached Beijing Normal University International Division