Line 228: | Line 228: | ||
pSBC3-PGK1 + DAK + TPFK1 and the YCplac33 plasmid vector to form intact plasmid with URA deficient. | pSBC3-PGK1 + DAK + TPFK1 and the YCplac33 plasmid vector to form intact plasmid with URA deficient. | ||
</h4><br> | </h4><br> | ||
− | <h4>2.pSBC3-PGK1+DAK+ | + | <h4>2.pSBC3-PGK1+DAK+tPFK1<br><h4> |
− | <h4>In this part, the promoter of DAK is PGK1 promoter, and the terminator is | + | <h4>In this part, the promoter of DAK is PGK1 promoter, and the terminator is tPFK1 terminator. PGK1 promoter |
− | is a kind of promoter which has strong expression and | + | is a kind of promoter which has strong expression and tPFK1 terminator is a terminator with rather strong |
expression ability. So they can both increase the expression of genes. At the same time they are constitutive | expression ability. So they can both increase the expression of genes. At the same time they are constitutive | ||
promoters and terminators, which have the ability to integrate gene fragment, DAK, into the chromosome | promoters and terminators, which have the ability to integrate gene fragment, DAK, into the chromosome |
Revision as of 19:37, 1 November 2017