Difference between revisions of "Team:SiCAU-China"

 
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<h1> description </h1>
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Antibiotic is one of the most popular drug in the world, it can be used in medical treatment, agriculture, food processing, research and so on. But overusing antibiotics may bring antibiotics residue to environment, which will cause potential damage to human and impact the ecotope. Nowadays there are some physical and chemical methods to detect antibiotics residue, but they often need high cost and complicated process. So this year our team focus on Biosensor, said to be a easier way to detect than traditional methods at a lower price, to realize the trace detection.
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The positive feedback loop has been widely applied in synthetic biology, such as logic gates, oscillators, bistable circuit. Besides, the positive feedback loop is also an ultra-sensitive circuit, just like Polymerase Chain Reaction (PCR), even be used in quantitative expression detected. So we engineered a biosensor, which will be established a positive feedback loop using luxI/luxR system in E.coli, to achieve trace detection. Similar to the detect theory of real-time quantitative PCR, there will be a relation between the content of input signal and the time to open the loop. We will use this system to do some antibiotics leftover detections.
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<div class="mao"><a href="#"><img src="https://static.igem.org/mediawiki/2017/b/bc/T-SICAU-sidebar_mao.jpg" />  <div class="t"><b>top</b></div></a></div>
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  <div id="team-icon"><a href="https://2017.igem.org/Team:SiCAU-China"> <img src="https://static.igem.org/mediawiki/2017/2/2c/T-SICAU-Sherlock_E.coli.jpg" alt="club's logo" /> </a></div>
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  <div class="navigation-menu">
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    <li><a href="#"><div>
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<img src="https://static.igem.org/mediawiki/2017/thumb/5/5b/T-SICAU-magnifier.jpg/180px-T-SICAU-magnifier.jpg.png" alt="project" class="up-nav-icon" />project</div></a>
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<a href="https://2017.igem.org/Team:SiCAU-China/Description">Description</a>  
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  <a href="https://2017.igem.org/Team:SiCAU-China/Results">Results</a>
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                  <a href="https://2017.igem.org/Team:SiCAU-China/Contribution">Contribution</a> 
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<li><a href="https://2017.igem.org/Team:SiCAU-China/Model"><div>
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<img src="https://static.igem.org/mediawiki/2017/thumb/c/c3/T-SICAU-letter_opener.jpg/180px-T-SICAU-letter_opener.jpg.png" alt="Model" class="up-nav-icon" />
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        Model</div></a>
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    <li><a href="https://2017.igem.org/Team:SiCAU-China/HP/Gold_Integrated"><div>
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<img src="https://static.igem.org/mediawiki/2017/thumb/7/77/T-SICAU-hat.jpg/105px-T-SICAU-hat.jpg.png" alt="practice" class="up-nav-icon" />
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    Human Practices</div></a>
  
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        <img src="https://static.igem.org/mediawiki/2017/thumb/a/ab/T-SICAU-door.jpg/180px-T-SICAU-door.jpg.png" alt="Notebook" class="up-nav-icon" />Notebook</div></a>
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        <a href="https://2017.igem.org/Team:SiCAU-China/Notebook">Notebook</a>
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        <a href="https://2017.igem.org/Team:SiCAU-China/Experiments">Protocol</a></div></li>
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<li><a href="#"><div>
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<img src="https://static.igem.org/mediawiki/2017/thumb/7/7f/T-SICAU-team.jpg/120px-T-SICAU-team.jpg.png" alt="team" class="up-nav-icon" />team</div></a>
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<a href="https://2017.igem.org/Team:SiCAU-China/Team">Team</a>
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                <a href="https://2017.igem.org/Team:SiCAU-China/Attributions">Attributions </a>
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                <a href="https://2017.igem.org/Team:SiCAU-China/gally">Gallery</a></div></li>
  
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        <li><a href="https://2017.igem.org/Team:SiCAU-China/Collaborations"><div>  
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<h1> Welcome to iGEM 2017! </h1>
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        <img src="https://static.igem.org/mediawiki/2017/thumb/a/a6/T-SICAU-pipe.jpg/180px-T-SICAU-pipe.jpg.png" alt="Collaborations" class="up-nav-icon" />
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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          Collaborations</div></a>
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<h5>Before you start: </h5>
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<p> Please read the following pages:</p>
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<li> <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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<h5> Styling your wiki </h5>
 
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
 
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
 
 
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<h5> Wiki template information </h5>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Positive feedback system can be found in everywhere, which exists widely in creatures ranging from Lac operon of prokaryotes to human beings.  It has been used to construct various attractive circuits such bistable state, oscillators and other inconceivable systems. In our project this year, the significant performance of sensitivity would be developed in the positive feedback loop established by LuxR/LuxI quorum sensing system. But this loop can be opened up because of its background expression. The AiiA, which can hydrolyze the AHL, together with the Lac operon, will control the background expression. Furthermore, the input defined by user will have a negative correlation to the opening time. Hence, there is a need to establish a model to describe this relation and even realize quantitative determination.
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<h5> Editing your wiki </h5>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h5>Inspiration</h5>
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<script type="text/javascript">
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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Latest revision as of 19:47, 1 November 2017

SiCAU-iGEM

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      Positive feedback system can be found in everywhere, which exists widely in creatures ranging from Lac operon of prokaryotes to human beings. It has been used to construct various attractive circuits such bistable state, oscillators and other inconceivable systems. In our project this year, the significant performance of sensitivity would be developed in the positive feedback loop established by LuxR/LuxI quorum sensing system. But this loop can be opened up because of its background expression. The AiiA, which can hydrolyze the AHL, together with the Lac operon, will control the background expression. Furthermore, the input defined by user will have a negative correlation to the opening time. Hence, there is a need to establish a model to describe this relation and even realize quantitative determination.





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