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class="img-responsive"></center> | class="img-responsive"></center> | ||
<h4> | <h4> | ||
− | Standardized curve of protein concentration | + | <center>Standardized curve of protein concentration</center> |
<br> The purified and quantified protein was used for enzymatic activity reaction. The control group | <br> The purified and quantified protein was used for enzymatic activity reaction. The control group | ||
was not add ceaS2 enzyme, and the protein buffer was used to make up the volume. The reaction system | was not add ceaS2 enzyme, and the protein buffer was used to make up the volume. The reaction system | ||
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<h4> | <h4> | ||
− | Identification function of ceaS2 by liquid chromatogram | + | <center>Identification function of ceaS2 by liquid chromatogram</center> |
<br> (The black line is acrylic acid standard, green line is control, blue line is reaction of D-G3P | <br> (The black line is acrylic acid standard, green line is control, blue line is reaction of D-G3P | ||
as substrate, red line is reaction DHAP as substrate) | as substrate, red line is reaction DHAP as substrate) | ||
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<h4> | <h4> | ||
− | LC-MS of ceaS2 generated acrylic acid | + | <center>LC-MS of ceaS2 generated acrylic acid</center> |
<br> (From top to bottom is acrylic acid standard, control, ceas2 reaction by DHAP as substrate, secondary | <br> (From top to bottom is acrylic acid standard, control, ceas2 reaction by DHAP as substrate, secondary | ||
mass spectrum of acrylic acid) | mass spectrum of acrylic acid) | ||
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must pass through, according to which we designed a new approach to acrylic acid biosynthesis based | must pass through, according to which we designed a new approach to acrylic acid biosynthesis based | ||
on glycerol. | on glycerol. | ||
− | <br> | + | <br> The new pathway, as shown below |
</h4> | </h4> | ||
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<h4> | <h4> | ||
− | The acrylic biosynthetic pathway based on E. coli glycerol aerobic metabolic pathway | + | <center>The acrylic biosynthetic pathway based on E. coli glycerol aerobic metabolic pathway</center> |
<br> This new approach is the shortest compared to other known acrylic pathways, where only three enzymes | <br> This new approach is the shortest compared to other known acrylic pathways, where only three enzymes | ||
are needed to achieve the synthesis of acrylic acid from glycerol, so this pathway not merely has | are needed to achieve the synthesis of acrylic acid from glycerol, so this pathway not merely has | ||
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inducing the expression of ceaS2 enzyme in E. coli, the reaction system was prepared and carried | inducing the expression of ceaS2 enzyme in E. coli, the reaction system was prepared and carried | ||
out. The reaction solution was filtered and the acrylic acid content was determined by high performance | out. The reaction solution was filtered and the acrylic acid content was determined by high performance | ||
− | liquid chromatography (HPLC). The results are as follows: | + | liquid chromatography (HPLC). The results are as follows:<br> |
− | <br> | + | <br> <center>Primitive peak figure of liquid phase, with standard control sample. Shown as follows</center> |
<br> | <br> | ||
Latest revision as of 20:58, 1 November 2017