Difference between revisions of "Team:NYU Abu Dhabi/Demonstrate"

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{{NYU_Abu_Dhabi}}
 
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<div class="column full_size judges-will-not-evaluate">
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    <link rel="stylesheet" href="https://2017.igem.org/Template:NYU_Abu_Dhabi/homeCSS?action=raw&ctype=text/css" />
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<h3>★  ALERT! </h3>
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    <!-- <link rel="stylesheet" href="./home.css" /> -->
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<div class="clear"></div>
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<div class="column full_size">
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  </head>
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<h1>Demonstrate</h1>
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  <body>
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<h3>Gold Medal Criterion #4</h3>
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    <div id="app">
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      <!-- Navbar -->
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      <nav class="navbar navbar-expand-lg fixed-top navbar-light bg-light">
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        <div class="collapse navbar-collapse" id="navbarNavDropdown">
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              <a class="nav-link" href="https://2017.igem.org/Team:NYU_Abu_Dhabi">Home <span class="sr-only">(current)</span></a>
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                Project
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                <a class="dropdown-item" href="https://2017.igem.org/Team:NYU_Abu_Dhabi/Description">Our Project</a>
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                <a class="dropdown-item" href="https://2017.igem.org/Team:NYU_Abu_Dhabi/Applied_Design">Design</a>
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              </div>
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            </li>
  
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<p>
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            <li class="nav-item">
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
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              <a class="nav-link" href="https://2017.igem.org/Team:NYU_Abu_Dhabi/Collaborations">Collaborations</a>
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</p>
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<p>
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Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
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</p>
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                Lab Notebook
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                <a class="dropdown-item" href="https://2017.igem.org/Team:NYU_Abu_Dhabi/Biology">Biology</a>
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                <a class="dropdown-item" href="https://2017.igem.org/Team:NYU_Abu_Dhabi/Engineering">Engineering</a>
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                <a class="dropdown-item" href="https://2017.igem.org/Team:NYU_Abu_Dhabi/Protocols">Protocols</a>
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                <a class="dropdown-item" href="https://2017.igem.org/Team:NYU_Abu_Dhabi/HP">Overview</a>
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                <a class="dropdown-item" href="https://2017.igem.org/Team:NYU_Abu_Dhabi/HP/Silver">Human Practices</a>
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                <a class="dropdown-item" href="https://2017.igem.org/Team:NYU_Abu_Dhabi/HP/Gold_Integrated">Integrated Human Practices</a>
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</div>
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            <li class="nav-item">
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              <a class="nav-link" href="https://2017.igem.org/Team:NYU_Abu_Dhabi/InterLab">Interlab</a>
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                Team
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<div class="column half_size">
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<img id="illustration-hero-image" src="https://static.igem.org/mediawiki/2017/d/dd/ProjDem.png"/>
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<div class="section-block">
  
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<h4> What should we do for our demonstration?</h4>
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<p class="section-content">
  
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<h5> Standard teams </h5>
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<div style="padding-top:5px">
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  <video width="660" height="450" controls>
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    <source src="https://static.igem.org/mediawiki/2017/2/26/Demovideo_final.mp4">
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  </video>
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</div>
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</p>
  
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<p>  
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<p class="section-content">
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If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
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We have demonstrated that our device works through three separate <a href="https://2017.igem.org/Team:NYU_Abu_Dhabi/Results">proofs of concept</a>. We have shown that our device has been able to detect our gene of interest, <i>rfbE</i> (<a href="http://parts.igem.org/cgi/partsdb/jumper.cgi?search=BBa_K2495001">BBa_K2495001</a>), as well as a genomic target for <i>Plasmodium falciparum</i>. Feedback from our target audiences has indicated that our prototype is 1) functional and 2) appropriate for its designated use and audience, as it is portable, simple and does not require any laboratory equipment.
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<b>
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The instructions specified in the video are provided below:
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</p>
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<p class="section-content">
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The provided kit contains all you need for rapid detection of shiga toxin-producing <i>E. coli</i>. Inside you'll find:
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<ul>
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<li> A cotton swab
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<li> 2 disposable Pasteur Pipettes
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<li> 2 solutions labelled "S" (for sample) and "C" (for control)
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<li> A PDMS chip
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<li> A small sheet of optical adhesive
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</ul>
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</p>
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<p class="section-content">
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To use the device simply follow the remaining steps:
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<ol type="1">
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<li> Insert the USB into any portable battery pack or into a USB-to-wall adaptor to begin warming the device.
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<li> Swab your food sample using the provided cotton swab and swirl it into the solution labelled "S".
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<li> Place the provided PDMS chip with the wells open towards you.
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<li> In the wells labelled "+" and "-", add the solution from the tube labelled "C" using one of the pipettes. Make sure to add enough volume to fill each well.
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<li> Taking the clean pipette, add your solution from the tube labelled "S" to the remaining wells.
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<li> Seal the top of the PDMS plate using the provided optical adhesive.
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<li> Insert the lid back onto the device and wait for 20 minutes.
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<li> After 20 minutes, you results are ready for visualization.
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</ol>
 
</p>
 
</p>
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</div>
 
  
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<div class="column half_size">
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<p class="section-content">
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If your samples are brighter than the well marked "-", your samples may be contaminated by Shiga toxin-producing <i>E. coli</i> and we suggest discarding them.  
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<br>
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<h5> Special track teams </h5>
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<p>
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Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
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              NYU Abu Dhabi is a research university with a fully integrated liberal arts and science college. It draws students from around the world, and prepares them for the challenges and opportunities of our interconnected world. </br> <a href="http://nyuad.nyu.edu/en/about.html">Read More</a>
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              NYUAD Saadiyat Island </br>
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              Abu Dhabi, P.O. Box 129188, U.A.E </br>
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              <a href="mailto:nyuad.igem@nyu.edu">Email</a> </br>
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Latest revision as of 21:05, 1 November 2017

We have demonstrated that our device works through three separate proofs of concept. We have shown that our device has been able to detect our gene of interest, rfbE (BBa_K2495001), as well as a genomic target for Plasmodium falciparum. Feedback from our target audiences has indicated that our prototype is 1) functional and 2) appropriate for its designated use and audience, as it is portable, simple and does not require any laboratory equipment. The instructions specified in the video are provided below:

The provided kit contains all you need for rapid detection of shiga toxin-producing E. coli. Inside you'll find:

  • A cotton swab
  • 2 disposable Pasteur Pipettes
  • 2 solutions labelled "S" (for sample) and "C" (for control)
  • A PDMS chip
  • A small sheet of optical adhesive

To use the device simply follow the remaining steps:

  1. Insert the USB into any portable battery pack or into a USB-to-wall adaptor to begin warming the device.
  2. Swab your food sample using the provided cotton swab and swirl it into the solution labelled "S".
  3. Place the provided PDMS chip with the wells open towards you.
  4. In the wells labelled "+" and "-", add the solution from the tube labelled "C" using one of the pipettes. Make sure to add enough volume to fill each well.
  5. Taking the clean pipette, add your solution from the tube labelled "S" to the remaining wells.
  6. Seal the top of the PDMS plate using the provided optical adhesive.
  7. Insert the lid back onto the device and wait for 20 minutes.
  8. After 20 minutes, you results are ready for visualization.

If your samples are brighter than the well marked "-", your samples may be contaminated by Shiga toxin-producing E. coli and we suggest discarding them.

NYU Abu Dhabi is a research university with a fully integrated liberal arts and science college. It draws students from around the world, and prepares them for the challenges and opportunities of our interconnected world.
Read More

NYUAD Saadiyat Island
Abu Dhabi, P.O. Box 129188, U.A.E
Email
Facebook