Difference between revisions of "Team:UNebraska-Lincoln/Composite Part"

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<h1 class=topOfPage style="font-family:'Josefin Slab';">Composite Parts</h1>
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2461000"><h3 class=leap>Composite bromoperoxidase (BBa_k2461000):</h3></a>
  
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<p>This part is a composite part made with the constitutive promoter BBa_j23119, the ribosome binding site BBa_j61100, the gene that codes for a Vanadium-Dependent bromoperoxidase enzyme, and the commonly used terminator BBa_B0015. This part was designed to produce bromoform inside the rumen of cattle. Bromoform has been proven to reduce methane emissions, but it is usually delivered using seaweed to indirectly change the microbiome of the cattle. The UNebraska-Lincoln iGEM team decided to use this part to directly change the microbiome of the cattle. More information can be found <a href="https://2017.igem.org/Team:UNebraska-Lincoln/Description#whatToDo">HERE</a>.</p>
  
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2461004"><h3 class=leap>nrfHA composite (BBa_k2461004):</h3></a>
  
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<p>This gene encodes both Cytochrome c552 (nrfA) and Cytochrome c small subunit (nrfH). The part includes the constitutive promoter J23100, the medium strength ribosome binding site B0032 before each coding sequence, and the double terminator B0015. This part was designed to convert nitrite into ammonia within the rumen of cattle. More information can be found <a href="https://2017.igem.org/Team:UNebraska-Lincoln/Description#whatToDo">HERE</a>.</p>
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<h1>Composite Parts</h1>
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
 
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
 
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<h3>Best Composite Part Special Prize</h3>
 
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<p>New BioBrick devices can be made by combining existing BioBrick Parts. For example, Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
 
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
 
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<h4>Note</h4>
 
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
 
  
 
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Latest revision as of 21:14, 1 November 2017

UNL 2017

Helping reduce methane emissions from livestock

Team Collaborations
Description Design Experiments Notebook InterLab Contribution Model Results Attributions
Parts Basic Parts Composite Parts
Safety
Silver HP Integrated and Gold

Composite Parts

Composite bromoperoxidase (BBa_k2461000):

This part is a composite part made with the constitutive promoter BBa_j23119, the ribosome binding site BBa_j61100, the gene that codes for a Vanadium-Dependent bromoperoxidase enzyme, and the commonly used terminator BBa_B0015. This part was designed to produce bromoform inside the rumen of cattle. Bromoform has been proven to reduce methane emissions, but it is usually delivered using seaweed to indirectly change the microbiome of the cattle. The UNebraska-Lincoln iGEM team decided to use this part to directly change the microbiome of the cattle. More information can be found HERE.

nrfHA composite (BBa_k2461004):

This gene encodes both Cytochrome c552 (nrfA) and Cytochrome c small subunit (nrfH). The part includes the constitutive promoter J23100, the medium strength ribosome binding site B0032 before each coding sequence, and the double terminator B0015. This part was designed to convert nitrite into ammonia within the rumen of cattle. More information can be found HERE.



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