Difference between revisions of "Team:Hamburg/Lab Results"

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     Biochemistry
 
     Biochemistry
 
   </h2>
 
   </h2>
 +
 +
In this project we were able to produce several parts, but unfortunately we only had enough time to charcterize some of them. To these belong promotor + tired + terminator and promtor + sfp, which were charcterized via kinetic measurements. The measured OD values before and after induction with 400 mM IPTG (+ 200 µg cystein) are shown in tables 1 and 2. </br>
 +
Table 1: OD values before induction
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<table border=2>
 +
  <tr> <th> </th>
 +
      <th> BL21 </th>
 +
      <th> tired </th>
 +
      <th> sfp </th> </tr>
 +
  <tr> <th> t = 0 </th>
 +
      <td> 0.1250</td>
 +
      <td> 0.0364 </td>
 +
      <td> 0.0260 </td> </tr>
 +
  <tr> <th> t = 70 </th>
 +
      <td> 0.3473</td>
 +
      <td> 0.0644 </td>
 +
      <td> 0.1154 </td> </tr>
 +
  <tr> <th> t = 120 </th>
 +
      <td> 1.0422</td>
 +
      <td> 0.3031 </td>
 +
      <td> 0.4058 </td> </tr>
 +
  <tr> <th> t = 180 </th>
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      <td> 1.5018</td>
 +
      <td> 0.4356 </td>
 +
      <td> 0.9930 </td> </tr>
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</table>
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Table 2: OD values after induction
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 +
 +
 
<h2 class="h2">Chemistry </h2>
 
<h2 class="h2">Chemistry </h2>
 
<h2 class="h2">Nanoscience</h2>
 
<h2 class="h2">Nanoscience</h2>

Revision as of 21:32, 1 November 2017

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Lab Results

Biochemistry

In this project we were able to produce several parts, but unfortunately we only had enough time to charcterize some of them. To these belong promotor + tired + terminator and promtor + sfp, which were charcterized via kinetic measurements. The measured OD values before and after induction with 400 mM IPTG (+ 200 µg cystein) are shown in tables 1 and 2.
Table 1: OD values before induction
BL21 tired sfp
t = 0 0.1250 0.0364 0.0260
t = 70 0.3473 0.0644 0.1154
t = 120 1.0422 0.3031 0.4058
t = 180 1.5018 0.4356 0.9930
Table 2: OD values after induction

Chemistry

Nanoscience

Sadly our first charge of microfluidic devices broke down when we tried to coat them with an ECM-solution to grow our cells on. And we were not able to produce a second charge before wiki freeze.


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