ShantiRicke (Talk | contribs) |
|||
Line 51: | Line 51: | ||
</td> | </td> | ||
<td> | <td> | ||
− | <a href="http://parts.igem.org/Part:BBa_K2401000">BBa_K2401000</a> | + | <a href="http://parts.igem.org/Part:BBa_K2401000"style="color: grey">BBa_K2401000</a> |
</td> | </td> | ||
<td> | <td> | ||
Line 68: | Line 68: | ||
</td> | </td> | ||
<td> | <td> | ||
− | <a href="http://parts.igem.org/Part:BBa_K2401005">BBa_K2401005</a> | + | <a href="http://parts.igem.org/Part:BBa_K2401005"style="color: grey">BBa_K2401005</a> |
</td> | </td> | ||
<td> | <td> | ||
Line 85: | Line 85: | ||
</td> | </td> | ||
<td> | <td> | ||
− | <a href="http://parts.igem.org/Part:BBa_K2401011">BBa_K2401011</a> | + | <a href="http://parts.igem.org/Part:BBa_K2401011"style="color: grey">BBa_K2401011</a> |
</td> | </td> | ||
<td> | <td> | ||
Line 102: | Line 102: | ||
</td> | </td> | ||
<td> | <td> | ||
− | <a href="http://parts.igem.org/Part:BBa_K2401012">BBa_K2401012</a> | + | <a href="http://parts.igem.org/Part:BBa_K2401012"style="color: grey">BBa_K2401012</a> |
</td> | </td> | ||
<td> | <td> | ||
Line 119: | Line 119: | ||
</td> | </td> | ||
<td> | <td> | ||
− | <a href="http://parts.igem.org/Part:BBa_K2401001">BBa_K2401001</a> | + | <a href="http://parts.igem.org/Part:BBa_K2401001"style="color: grey">BBa_K2401001</a> |
</td> | </td> | ||
<td> | <td> | ||
Line 136: | Line 136: | ||
</td> | </td> | ||
<td> | <td> | ||
− | <a href="http://parts.igem.org/Part:BBa_K2401002">BBa_K2401002</a> | + | <a href="http://parts.igem.org/Part:BBa_K2401002"style="color: grey">BBa_K2401002</a> |
</td> | </td> | ||
<td> | <td> | ||
Line 153: | Line 153: | ||
</td> | </td> | ||
<td> | <td> | ||
− | <a href="http://parts.igem.org/Part:BBa_K2401003">BBa_K2401003</a> | + | <a href="http://parts.igem.org/Part:BBa_K2401003"style="color: grey">BBa_K2401003</a> |
</td> | </td> | ||
<td> | <td> | ||
Line 170: | Line 170: | ||
</td> | </td> | ||
<td> | <td> | ||
− | <a href="http://parts.igem.org/Part:BBa_K2401004">BBa_K2401004</a> | + | <a href="http://parts.igem.org/Part:BBa_K2401004"style="color: grey">BBa_K2401004</a> |
</td> | </td> | ||
<td> | <td> | ||
Line 187: | Line 187: | ||
</td> | </td> | ||
<td> | <td> | ||
− | <a href="http://parts.igem.org/Part:BBa_K2401006">BBa_K2401006</a> | + | <a href="http://parts.igem.org/Part:BBa_K2401006"style="color: grey">BBa_K2401006</a> |
</td> | </td> | ||
<td> | <td> | ||
Line 204: | Line 204: | ||
</td> | </td> | ||
<td> | <td> | ||
− | <a href="http://parts.igem.org/Part:BBa_K2401007">BBa_K2401007</a> | + | <a href="http://parts.igem.org/Part:BBa_K2401007"style="color: grey">BBa_K2401007</a> |
</td> | </td> | ||
<td> | <td> | ||
Line 221: | Line 221: | ||
</td> | </td> | ||
<td> | <td> | ||
− | <a href="http://parts.igem.org/Part:BBa_K2401008">BBa_K2401008</a> | + | <a href="http://parts.igem.org/Part:BBa_K2401008"style="color: grey">BBa_K2401008</a> |
</td> | </td> | ||
<td> | <td> | ||
Line 238: | Line 238: | ||
</td> | </td> | ||
<td> | <td> | ||
− | <a href="http://parts.igem.org/Part:BBa_K2401010">BBa_K2401010</a> | + | <a href="http://parts.igem.org/Part:BBa_K2401010"style="color: grey">BBa_K2401010</a> |
</td> | </td> | ||
<td> | <td> | ||
Line 255: | Line 255: | ||
</td> | </td> | ||
<td> | <td> | ||
− | <a href="http://parts.igem.org/Part:BBa_K2401009">BBa_K2401009</a> | + | <a href="http://parts.igem.org/Part:BBa_K2401009"style="color: grey">BBa_K2401009</a> |
</td> | </td> | ||
<td> | <td> | ||
Line 272: | Line 272: | ||
</td> | </td> | ||
<td> | <td> | ||
− | <a href="http://parts.igem.org/Part:BBa_K2401013">BBa_K2401013</a> | + | <a href="http://parts.igem.org/Part:BBa_K2401013"style="color: grey">BBa_K2401013</a> |
</td> | </td> | ||
<td> | <td> | ||
Line 289: | Line 289: | ||
</td> | </td> | ||
<td> | <td> | ||
− | <a href="http://parts.igem.org/Part:BBa_K2401014">BBa_K2401014</a> | + | <a href="http://parts.igem.org/Part:BBa_K2401014"style="color: grey">BBa_K2401014</a> |
</td> | </td> | ||
<td> | <td> | ||
Line 340: | Line 340: | ||
<tr> | <tr> | ||
<td>Origin: </td> | <td>Origin: </td> | ||
− | <td> Pseudomonas aeruginosa PAO1 (strain: PAO1) </td> | + | <td> <i>Pseudomonas aeruginosa PAO1</i> (strain: PAO1) </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Line 353: | Line 353: | ||
<td>Function: </td> | <td>Function: </td> | ||
<td>Pyochelin-Complex Regulated Promotor, most likely a leaky promoter that induces the Pyochelin synthesis if Pyochelin-iron3+-di-complex binds to the receptor. Most siderophore receptor complexes need to be leaky since siderophores are only necessary under certain circumstances and self-induced. So, in the context of siderophores, leakiness is no flaw but a feature, including a biosensor for iron3+-ions in the expression system which makes sure that unnecessary metabolic load is exhibited. Therefore, the iron acquisition method is highly efficient and only expressed when the circumstances demand it. | <td>Pyochelin-Complex Regulated Promotor, most likely a leaky promoter that induces the Pyochelin synthesis if Pyochelin-iron3+-di-complex binds to the receptor. Most siderophore receptor complexes need to be leaky since siderophores are only necessary under certain circumstances and self-induced. So, in the context of siderophores, leakiness is no flaw but a feature, including a biosensor for iron3+-ions in the expression system which makes sure that unnecessary metabolic load is exhibited. Therefore, the iron acquisition method is highly efficient and only expressed when the circumstances demand it. | ||
− | Our goal was to characterize the promoter and measure if pyochelin-gallium3+ and Nor-pyochelin-gallium3+ complexes induce the native promoter to estimate the metabolic excess overload that our antibiotic-compound induces on native Pseudomonas aeruginosa. | + | Our goal was to characterize the promoter and measure if pyochelin-gallium3+ and Nor-pyochelin-gallium3+ complexes induce the native promoter to estimate the metabolic excess overload that our antibiotic-compound induces on native <i>Pseudomonas aeruginosa</i>. |
</td> | </td> | ||
</tr> | </tr> |
Revision as of 22:07, 1 November 2017
Parts
Full Part table
|
Part Number | Type | Designer | Length |
---|---|---|---|---|
Nor Pyochelin Synthesis Cluster | BBa_K2401000 | Composite | A. Nicolai, S. Ricke | 10760 bp |
Yersiniabactin inner Membrane Transporter | BBa_K2401005 | Composite | A. Nicolai, S. Ricke | 3956 bp |
Siderophore outer Membrane Transporter | BBa_K2401011 | Composite | A. Nicolai, S. Ricke | 5724 bp |
HMWP1 Yersiniabactin Synthetase part 2 | BBa_K2401012 | Composite | A. Nicolai, S. Ricke | 10731 bp |
HMWP2 Yersiniabactin Synthetase Part 1 | BBa_K2401001 | Basic | A. Nicolai, S. Ricke | 6107 bp |
2,3-Dihydroxybenzoate-AMP ligase | BBa_K2401002 | Basic | A. Nicolai, S. Ricke | 1578 bp |
Thiazolinyl imide reductase | BBa_K2401003 | Basic | A. Nicolai, S. Ricke | 1101 bp |
Surfactin imide reductase | BBa_K2401004 | Basic | A. Nicolai, S. Ricke | 498 bp |
ABC-Siderophore-Transporter | BBa_K2401006 | Basic | A. Nicolai, S. Ricke | 1789 bp |
ATP-Siderophore-Bindingpermease | BBa_K2401007 | Basic | A. Nicolai, S. Ricke | 1801 bp |
HMWP1 Yersiniabactin Synthetase Part 2 | BBa_K2401008 | Basic | A. Nicolai, S. Ricke | 9492 bp |
FyuA TonB Yersiniabactin outermembrane Receptor | BBa_K2401010 | Basic | A. Nicolai, S. Ricke | 2022 bp |
FptA Pyochelin outermembrane Receptor | BBa_K2401009 | Basic | A. Nicolai, S. Ricke | 2160 bp |
PchR Pyochelin regulator | BBa_K2401013 | Regulatory | A. Nicolai, S. Ricke | 960 bp |
AraC like Yersiniabactin regulator | BBa_K2401014 | Regulatory | A. Nicolai, S. Ricke | 891 bp |
Basic Parts
AraC family transcriptional regulator [AraCftr] | |
---|---|
Origin: | Escherichia coli |
Data: | 891 bp, 296 AS, 32,3 kDa |
Part No.: | BBa_K2401014 |
Function: | Yersiniabactin-Complex Regulated Promotor, most likely a leaky promoter that induces the Yersiniabactin synthesis if Yersiniabactin-iron3+-complex binds to the receptor. Most siderophore receptor complexes need to be leaky since siderophores are only necessary under certain circumstances and self-induced. So, in the context of siderophores, leakiness is no flaw but a feature, including a biosensor for iron3+-ions in the expression system which makes sure that unnecessary metabolic load is exhibited. Therefore, the iron acquisition method is highly efficient and only expressed when the circumstances demand it. |
Plasmid: | Singular on a plasmid (pSB1C3) with cAMP-resistance. |
Regulatory protein PchR [PchR] | |
---|---|
Origin: | Pseudomonas aeruginosa PAO1 (strain: PAO1) |
Data: | 960 bp, 319 AS, 35.9 kDa |
Part No.: | BBa_K2401013 |
Function: | Pyochelin-Complex Regulated Promotor, most likely a leaky promoter that induces the Pyochelin synthesis if Pyochelin-iron3+-di-complex binds to the receptor. Most siderophore receptor complexes need to be leaky since siderophores are only necessary under certain circumstances and self-induced. So, in the context of siderophores, leakiness is no flaw but a feature, including a biosensor for iron3+-ions in the expression system which makes sure that unnecessary metabolic load is exhibited. Therefore, the iron acquisition method is highly efficient and only expressed when the circumstances demand it. Our goal was to characterize the promoter and measure if pyochelin-gallium3+ and Nor-pyochelin-gallium3+ complexes induce the native promoter to estimate the metabolic excess overload that our antibiotic-compound induces on native Pseudomonas aeruginosa. |
Plasmid: | Singular: pSB1C3 with cAMP-resistance. Combination: With HMWP2 , tired, sfp and dhBe to mimic the native expression system for pyochelin to measure additional metabolic load. |
Composite Parts