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<td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2347005">BBa_K2347005</a></td> | <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2347005">BBa_K2347005</a></td> | ||
<td>composite</td> | <td>composite</td> | ||
− | <td> | + | <td>DHAP/G3P->acrylic acid</td> |
<td>2862bp</td> | <td>2862bp</td> | ||
</tr> | </tr> | ||
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<td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2347006">BBa_K2347006</a></td> | <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2347006">BBa_K2347006</a></td> | ||
<td>composite</td> | <td>composite</td> | ||
− | <td> | + | <td>glycerol->DHA</td> |
− | <td> | + | <td>2862bp</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2347007">BBa_K2347007</a></td> | <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2347007">BBa_K2347007</a></td> | ||
<td>composite</td> | <td>composite</td> | ||
− | <td> | + | <td>DHA->DHAP</td> |
− | <td> | + | <td>2862bp</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2347008">BBa_K2347008</a></td> | <td><a target="_blank" href="http://parts.igem.org/Part:BBa_K2347008">BBa_K2347008</a></td> | ||
<td>composite</td> | <td>composite</td> | ||
− | <td> | + | <td>NADH->NAD+</td> |
− | <td> | + | <td>2862bp</td> |
</tr> | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
− | <h4>1.pSBC3- | + | <h4> |
− | + | ||
− | </h4><br> | + | <div class="col-md-12" style="padding-top:30px"> |
− | <h4>2.pSBC3- | + | <div class="col-md-6"> |
− | + | <center><h4>1.pSBC3-ADH1+gld+tGPD1<br><h4></center> | |
− | </h4><br> | + | <center> <h4>In this part, the promoter of gld is ADH1 promoter, and the terminator is tGPD1 terminator. ADH1 promoter is |
− | <h4> | + | a kind of promoter which has strong expression and tGPD1 terminator is a terminator with rather strong |
+ | expression ability. So they can both increase the expression of genes. At the same time they are constitutive | ||
+ | promoters and terminators, which have the ability to integrate gene fragment, gld, into the chromosome of | ||
+ | yeast, thereby reducing the burden of plasmid expression in yeast. We linked this part with part | ||
+ | pSBC3-PGK1 + DAK + TPFK1 and the YCplac33 plasmid vector to form intact plasmid with URA deficient. | ||
+ | </h4><br></center> | ||
+ | <center> <img src="https://static.igem.org/mediawiki/2017/e/e5/NPU-pSB1C3-ADH1-GlyDH-tGPD1.png" class="img-responsive"></center> | ||
+ | <h4> </h4> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="col-md-6"> | ||
+ | |||
+ | <center> <h4>2.pSBC3-PGK1+DAK+TPFK1<br><h4></center> | ||
+ | <center> <h4>In this part, the promoter of DAK is PGK1 promoter, and the terminator is TPFK1 terminator. PGK1 promoter | ||
+ | is a kind of promoter which has strong expression and TPFK1 terminator is a terminator with rather strong | ||
+ | expression ability. So they can both increase the expression of genes. At the same time they are constitutive | ||
+ | promoters and terminators, which have the ability to integrate gene fragment, DAK, into the chromosome | ||
+ | of yeast, thereby reducing the burden of plasmid expression in yeast. We linked this part with part | ||
+ | pSBC3-ADH1+gld+tGPD1 and the YCplac33 plasmid vector to form intact plasmid with URA deficient. | ||
+ | </h4><br></center> | ||
+ | |||
+ | <center> <img src="https://static.igem.org/mediawiki/2017/e/e8/PSB1C3-PGK1-DAK-tPFK1.png" class="img-responsive"></center> | ||
+ | <h4> </h4> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | <center> <h4>pSBC3-ADH1+gld+tGPD1和pSBC3-PGK1+DAK+TPFK1<br><h4></center> | ||
+ | <h4>The enzyme GlyDH (gld) and DAK can be efficiently expressed in the yeast cell, and the gene of the | ||
+ | enzyme GlyDH (gld) and DAK are integrated into the chromosome. In this pathway, GlyDH (Glycerol | ||
+ | dehydrogenase) can efficiently convert glycerol to DHA (1,3-Dihydroxyacetone), and then DAK | ||
+ | (Dihydroxyacetone kinase) phosphorylate DHA into DHAP, and then DHAP is catalyzed into acrylic acid | ||
+ | by ceaS2. | ||
+ | </h4> | ||
+ | <center> <img src="https://static.igem.org/mediawiki/2017/6/65/YCplac33-URA-gld-DAK.png" class="img-responsive"></center> | ||
+ | |||
+ | </h4> | ||
+ | <h4>3.pSBC3-pTDH3+ceas2+tPFK1<br><h4> | ||
<h4>In this part, the promoter of ceaS2 is pTDH3 promoter, and the terminator is tPFK1 terminator. pTDH3 | <h4>In this part, the promoter of ceaS2 is pTDH3 promoter, and the terminator is tPFK1 terminator. pTDH3 | ||
promoter is a kind of promoter which has strong expression and tPFK1 terminator is a terminator with | promoter is a kind of promoter which has strong expression and tPFK1 terminator is a terminator with | ||
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with the YCplac33 plasmid vector to form intact plasmid with LEU deficient. | with the YCplac33 plasmid vector to form intact plasmid with LEU deficient. | ||
</h4><br> | </h4><br> | ||
− | <h4>4.pSBC3-TEF2+NOX+tRPS2 | + | <h4>4.pSBC3-TEF2+NOX+tRPS2<br><h4> |
− | <h4>In this part, the promoter of NOX is TEF2 promoter, and the terminator is tRPS2 terminator. TEF2 promoter is a kind of promoter which has strong expression and tRPS2 terminator is a terminator with rather strong expression ability. So they can both increase the expression of genes. At the same time they are constitutive promoters and terminators, which have the ability to integrate gene fragment, NOX, into the chromosome of yeast, thereby reducing the burden of plasmid expression in yeast. We linked this part with part pSBC3-pTDH3+ceas2+ | + | <h4>In this part, the promoter of NOX is TEF2 promoter, and the terminator is tRPS2 terminator. TEF2 promoter is |
+ | a kind of promoter which has strong expression and tRPS2 terminator is a terminator with rather strong | ||
+ | expression ability. So they can both increase the expression of genes. At the same time they are constitutive | ||
+ | promoters and terminators, which have the ability to integrate gene fragment, NOX, into the chromosome | ||
+ | of yeast, thereby reducing the burden of plasmid expression in yeast. We linked this part with part | ||
+ | pSBC3-pTDH3+ceas2+tPFK1and the YCplac33 plasmid vector to form intact plasmid with LEU deficient. | ||
+ | </h4><br> | ||
+ | <h4>4.pSBC3-TEF2+NOX+tRPS2<br><h4> | ||
+ | <h4>In this part, the promoter of NOX is TEF2 promoter, and the terminator is tRPS2 terminator. TEF2 promoter is | ||
+ | a kind of promoter which has strong expression and tRPS2 terminator is a terminator with rather strong | ||
+ | expression ability. So they can both increase the expression of genes. At the same time they are constitutive | ||
+ | promoters and terminators, which have the ability to integrate gene fragment, NOX, into the chromosome | ||
+ | of yeast, thereby reducing the burden of plasmid expression in yeast. We linked this part with part | ||
+ | pSBC3-pTDH3+ceas2+tPFK1and the YCplac33 plasmid vector to form intact plasmid with LEU deficient. | ||
</h4><br> | </h4><br> | ||
− | + | ||
− | + | ||
<h4>1.pSBC3-pTDH3+ceas2+tPFK1<br><h4> | <h4>1.pSBC3-pTDH3+ceas2+tPFK1<br><h4> | ||
<h4>In the yeast cells, the enzyme ceaS2 can be efficiently expressed and the gene of ceas2 is integrated into | <h4>In the yeast cells, the enzyme ceaS2 can be efficiently expressed and the gene of ceas2 is integrated into | ||
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(glyceraldehyde 3-phosphate ) as substrate. | (glyceraldehyde 3-phosphate ) as substrate. | ||
</h4><br> | </h4><br> | ||
− | <h4>2.pSBC3-ADH1+gld+ | + | <h4>2.pSBC3-ADH1+gld+tGPD1和pSBC3-PGK1+DAK+TPFK1<br><h4> |
<h4>The enzyme GlyDH (gld) and DAK can be efficiently expressed in the yeast cell, and the gene of the | <h4>The enzyme GlyDH (gld) and DAK can be efficiently expressed in the yeast cell, and the gene of the | ||
enzyme GlyDH (gld) and DAK are integrated into the chromosome. In this pathway, GlyDH (Glycerol | enzyme GlyDH (gld) and DAK are integrated into the chromosome. In this pathway, GlyDH (Glycerol |
Revision as of 22:29, 1 November 2017