Difference between revisions of "Team:Wageningen UR/Achievements"

 
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{{Wageningen_UR/MobileMenu}}
 
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{{Wageningen_UR/NotebookCSS}}
  
 
<html>
 
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<div class="container-fluid OurContent">
 
<div class="container-fluid OurContent">
  
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                         <ul class="breadcrumb">
 
                         <ul class="breadcrumb">
 
                             <li><a href="https://2017.igem.org/Team:Wageningen_UR">Home</a></li>
 
                             <li><a href="https://2017.igem.org/Team:Wageningen_UR">Home</a></li>
−
                             <li><a href="https://2017.igem.org/Team:Wageningen_UR/Achievements">Achievements</a></li>
+
                             <li><a href="https://2017.igem.org/Team:Wageningen_UR/Notebook">Notebook</a></li>
 
                             <li>Achievements</li>
 
                             <li>Achievements</li>
 
                         </ul>
 
                         </ul>
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                                 <h1>Achievements</h1> </div>
 
                                 <h1>Achievements</h1> </div>
  
−
                           <p>This year's Wageningen iGEM team has been working hard and while doing so has kept the medal <a href="https://2017.igem.org/Judging/Medals">requirements</a> in mind. Below each medal a list can be found which in our opinion completes the criteria that have to be met to earn the medals.</p>
+
                           <p>This year's Wageningen iGEM team has been working hard and while doing so has kept the medal <a href="https://2017.igem.org/Judging/Medals">requirements</a> in mind. Below each medal, a list can be found which completes the criteria that have to be met to earn the medals.</p>
 
   
 
   
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<div class="figure-fullwidth">                    
 
<div class="figure-fullwidth">                    
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</div></div>
 
</div></div>
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<ul>
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<br>
−
<li>Integrated Human Practices:</li>
+
−
<li>Improve a previous part or project</li>
+
<ul style="border:10px; border-style:solid; border-color:#ffd708; padding: 1em; border-radius: 10px;">
−
<li>Advanced <a href="https://2017.igem.org/Team:Wageningen_UR/Model">models</a> were created and used to get insight into the  <a href="https://2017.igem.org/Team:Wageningen_UR/Model/QS">quorum sensing</a> and <a href="https://2017.igem.org/Team:Wageningen_UR/Model/Kinetics">reporter system</a> parts of our project.</li>
+
<li>Advanced <a href="https://2017.igem.org/Team:Wageningen_UR/Model">models</a> were created and used to get insight into the  <a href="https://2017.igem.org/Team:Wageningen_UR/Model/QS">quorum sensing</a> and <a href="https://2017.igem.org/Team:Wageningen_UR/Model/Cpx_Kinetics">reporter system</a> parts of our project.</li>
−
<li>Demonstrate your work</li>
+
<li>Integrated Human Practices:</li>
−
</ul>
+
<ul style="list-style-type:none">
 +
<li>
 +
 +
We consulted with experts from the Dutch National Institute of Public Health and the Environment (RIVM), the corporate Foundation for Innovative New Diagnostics in Belgium, workers of the Médecines sans Frontières (MSF/Doctors without Borders) organization, a philosophy professor and many more. With the insights gained from experts in the field of diagnostics and people from MSF in Africa and Brazil we were able to improve the diagnostic device to suit the requirements of such a device. We were also able to take the biosafety aspect of our project into account and even consider the ethical dillemma's that arise when implementing a new diagnostic device.</li>
 +
</ul>
 +
<li>We improved BioBricks <a href=http://parts.igem.org/Part:BBa_I715022>BBa_I715022</a>  & <a href=http://parts.igem.org/Part:BBa_I715023>BBa_I715023</a>  by changing the incorrect split site AA155 to the correct split site AA168 <a href=http://parts.igem.org/Part:BBa_K2387055>BBa_K2387055</a>.</li>
 
 
 +
<li>During our project we <a href=https://2017.igem.org/Team:Wageningen_UR/Demonstrate>demonstrated</a> that Mantis is viable in blood serum dilutions, and that fluorescence can indeed be measured in blood serum.
 +
Furthermore, we performed experiments to consistently air-dry our cells, and we combined several wet-lab project to perfect the functionality of Mantis.
 +
</li>
 +
</ul>
 +
<br>
 
<div class="figure-fullwidth">                    
 
<div class="figure-fullwidth">                    
 
                <img class="figure-fullwidth-img" src="https://static.igem.org/mediawiki/2017/2/2f/Silver_medal_wur.png" style="width: 30%"/>
 
                <img class="figure-fullwidth-img" src="https://static.igem.org/mediawiki/2017/2/2f/Silver_medal_wur.png" style="width: 30%"/>
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</div>
 
</div>
−
<ul>
+
<br>
 +
 +
<ul style="border:10px; border-style:solid; border-color:#989188; padding: 1em; border-radius: 10px;">
 
<li>Validated the functionality of the following submitted BioBricks:</li>
 
<li>Validated the functionality of the following submitted BioBricks:</li>
 
<div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
 
<div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
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                                         <h4 class="panel-title">
 
                                         <h4 class="panel-title">
 
<div class="col-xs-11">
 
<div class="col-xs-11">
−
Approach
+
Biobricks
 
</div><div class="col-xs-1"><i class="fa fa-arrow-down" aria-hidden="true"></i></div></a></h4>
 
</div><div class="col-xs-1"><i class="fa fa-arrow-down" aria-hidden="true"></i></div></a></h4>
 
</div>
 
</div>
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                </div>
 
                </div>
−
<ul>
+
<table style="width:100%">
−
<li><a href=http://parts.igem.org/Part:BBa_K2387060>BBa_K2387060</a></li>
+
<tr><th colspan="3"><i>T. brucei</i> antigens</th></tr>
−
<li><a href=http://parts.igem.org/Part:BBa_K2387061>BBa_K2387061</a></li>
+
                                        <tr>
−
<li><a href=http://parts.igem.org/Part:BBa_K2387014>BBa_K2387014</a></li>
+
                                            <th>BioBrick Code</th>
−
<li><a href=http://parts.igem.org/Part:BBa_K2387017>BBa_K2387017</a></li>
+
                                            <th>Part Name</th>
−
<li><a href=http://parts.igem.org/Part:BBa_K2387018>BBa_K2387018</a></li>
+
                                            <th>Designer</th>
−
<li><a href=http://parts.igem.org/Part:BBa_K2387022>BBa_K2387022</a></li>
+
                                        </tr>
−
<li><a href=http://parts.igem.org/Part:BBa_K2387023>BBa_K2387023</a></li>
+
                                        <tr>
−
<li><a href=http://parts.igem.org/Part:BBa_K2387024>BBa_K2387024</a></li>
+
                                            <td> <a href=http://parts.igem.org/Part:BBa_K2387060>BBa_K2387060</a></td>
−
<li><a href=http://parts.igem.org/Part:BBa_K2387001>BBa_K2387001</a></li>
+
                                            <td>Double tagged ISG64 of <i>T. brucei</i> gambiense</td>
−
<li><a href=http://parts.igem.org/Part:BBa_K2387045>BBa_K2387045</a></li>
+
                                            <td>Linda</td>
−
<li><a href=http://parts.igem.org/Part:BBa_K2387046>BBa_K2387046</a></li>
+
                                        </tr>
−
<li><a href=http://parts.igem.org/Part:BBa_K2387047>BBa_K2387047</a></li>
+
                                        <tr>
−
<li><a href=http://parts.igem.org/Part:BBa_K2387048>BBa_K2387048</a></li>
+
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387061>BBa_K2387061</a></td>
−
<li><a href=http://parts.igem.org/Part:BBa_K2387053>BBa_K2387053</a></li>
+
                                            <td>Double tagged ISG65 of <i>T. brucei</i> gambiense</td>
−
<li><a href=http://parts.igem.org/Part:BBa_K2387054>BBa_K2387054</a></li>
+
                                            <td>Linda</td>
−
<li><a href=http://parts.igem.org/Part:BBa_K2387055>BBa_K2387055</a></li>
+
                                        </tr>
−
<li><a href=http://parts.igem.org/Part:BBa_K2387003>BBa_K2387003</a></li>
+
                                    </table>
−
<li><a href=http://parts.igem.org/Part:BBa_K2387007>BBa_K2387007</a></li>
+
−
<li><a href=http://parts.igem.org/Part:BBa_K2387060>BBa_K2387008</a></li>
+
−
<li><a href=http://parts.igem.org/Part:BBa_K2387009>BBa_K2387009</a></li>
+
−
<li><a href=http://parts.igem.org/Part:BBa_K2387062>BBa_K2387062</a></li>
+
−
<li><a href=http://parts.igem.org/Part:BBa_K2387063>BBa_K2387063</a></li>
+
−
<li><a href=http://parts.igem.org/Part:BBa_K2387065>BBa_K2387065</a></li>
+
−
<li><a href=http://parts.igem.org/Part:BBa_K2387010>BBa_K2387010</a></li>
+
−
<li><a href=http://parts.igem.org/Part:BBa_K2387029>BBa_K2387029</a></li>
+
−
<li><a href=http://parts.igem.org/Part:BBa_K2387030>BBa_K2387030</a></li>
+
−
<li><a href=http://parts.igem.org/Part:BBa_K2387031>BBa_K2387031</a></li>
+
−
<li><a href=http://parts.igem.org/Part:BBa_K2387032>BBa_K2387032</a></li>
+
−
<li><a href=http://parts.igem.org/Part:BBa_K2387074>BBa_K2387074</a></li>
+
−
<li> </li>
+
−
<li> </li>
+
−
<li> </li>
+
−
+
−
+
−
</ul>
+
−
<li><a href="https://2017.igem.org/Team:Wageningen_UR/Collaborations">Collaborated</a> with the Utrecht and TU Delft iGEM teams</li>
+
−
<li>Organised and hosted the first BeNeLux iGEM <a href="https://2017.igem.org/Team:Wageningen_UR/HP/Meetup">meet-up</a></li>
+
−
<li>Participated and completed a RIVM project on <a href="https://2017.igem.org/Team:Wageningen_UR/Safety">biosafety</a>, which was rewarded with a grant.</li>
+
−
</ul>
+
  
 +
           
 +
                                    <table style="width:100%">
 +
<tr><th colspan="3">Cpx System</th></tr>                                       
 +
<tr>
 +
                                            <th>BioBrick Code</th>
 +
                                            <th>Part Name</th>
 +
                                            <th>Designer</th>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387014>BBa_K2387014</a></td>
 +
                                            <td>Cpx system reporter + CpxP inhibitor</td>
 +
                                            <td>Stijn</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387017>BBa_K2387017</a></td>
 +
                                            <td>Cpx system reporter + CpxP-Affinity molecule inhibitor</td>
 +
                                            <td>Stijn</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387018>BBa_K2387018</a></td>
 +
                                            <td>Cpx system reporter + Affinity molecule-CpxP inhibitor</td>
 +
                                            <td>Stijn</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387022>BBa_K2387022</a></td>
 +
                                            <td>Cpx system reporter + tethered CpxP</td>
 +
                                            <td>Stijn</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387023>BBa_K2387023</a></td>
 +
                                            <td>Cpx system reporter + tethered CpxP-Affinity molecule</td>
 +
                                            <td>Stijn</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387024>BBa_K2387024</a></td>
 +
                                            <td>Cpx system reporter + tethered Affinity molecule-CpxP</td>
 +
                                            <td>Stijn</td>
 +
                                        </tr>
 +
                                    </table>
 +
 +
                                    <table style="width:100%">
 +
<tr><th colspan="3">Reporters and Split Reporters</th></tr>
 +
                                        <tr>
 +
                                            <th>BioBrick Code</th>
 +
                                            <th>Part Name</th>
 +
                                            <th>Designer</th>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387001>BBa_K2387001</a></td>
 +
                                            <td>Monomeric chromoprotein anm2CP controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>José Manuel</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387045>BBa_K2387045</a></td>
 +
                                            <td>mVenus controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>José Manuel</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387046>BBa_K2387046</a></td>
 +
                                            <td>Split mVenus controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>José Manuel</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387047>BBa_K2387047</a></td>
 +
                                            <td>sfGFP controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>José Manuel</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387048>BBa_K2387048</a></td>
 +
                                            <td>Split sfGFP controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>José Manuel</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387052>BBa_K2387052</a></td>
 +
                                            <td>mCerulean controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>José Manuel</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387053>BBa_K2387053</a></td>
 +
                                            <td>Split mCerulean controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>José Manuel</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387054>BBa_K2387054</a></td>
 +
                                            <td>mRFP controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>José Manuel</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387055>BBa_K2387055</a></td>
 +
                                            <td>Split mRFP controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>José Manuel</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387003>BBa_K2387003</a></td>
 +
                                            <td>eYFP controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>Bart</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387007>BBa_K2387007</a></td>
 +
                                            <td>eYFPn [1-154] controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>Bart</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387060>BBa_K2387008</a></td>
 +
                                            <td>eYFPc [155-238] controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>Bart</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387009>BBa_K2387009</a></td>
 +
                                            <td>eYFPn [1-154] and eYFPc[155-238] controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>Bart</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387062>BBa_K2387062</a></td>
 +
                                            <td>eYFP[155-238]-cLeucine Zipper fusion controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>Bart</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387063>BBa_K2387063</a></td>
 +
                                            <td>eYFP[1-154]-nLeucine Zipper fusion controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>Bart</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387065>BBa_K2387065</a></td>
 +
                                            <td>eYFP[1-154]-nLeucine Zipper and eYFP[155-238]-cLeucine Zipper fusions controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>Bart</td>
 +
                                        </tr>
 +
                                    </table>
 +
 +
                 
 +
                                    <table style="width:100%">
 +
<tr><th colspan="3">Cpx + BiFC</th></tr>
 +
                                        <tr>
 +
                                            <th>BioBrick Code</th>
 +
                                            <th>Part Name</th>
 +
                                            <th>Designer</th>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387010>BBa_K2387010</a></td>
 +
                                            <td>CpxA-eYFPn fusion controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>Bart</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387029>BBa_K2387029</a></td>
 +
                                            <td>CpxR-mVenusn and CpxR-mVenusc fusions controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>Bart</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387030>BBa_K2387030</a></td>
 +
                                            <td>CpxR-eYFPc fusion controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>Bart</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387031>BBa_K2387031</a></td>
 +
                                            <td>CpxR-eYFPn fusion controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>Bart</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387032>BBa_K2387032</a></td>
 +
                                            <td>CpxR-eYFPn and CpxR-eYPFc fusions controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>Bart</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387074>BBa_K2387074</a></td>
 +
                                            <td>TEV protease fused to CpxR controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>Bart</td>
 +
                                        </tr>
 +
                                    </table>
 +
                               
 +
                                    <table style="width:100%">
 +
<tr><th colspan="3">Quorum Sensing System</th></tr>
 +
                                        <tr>
 +
                                            <th>BioBrick Code</th>
 +
                                            <th>Part Name</th>
 +
                                            <th>Designer</th>
 +
                                        </tr>
 +
<tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387066>BBa_K2387066</a></td>
 +
                                            <td>Colicin E7 lytic mechanism controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>Natalia</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387068>BBa_K2387068</a></td>
 +
                                            <td>T4 Holin lytic mechanism controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>Natalia</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387069>BBa_K2387069</a></td>
 +
                                            <td>T4 Endolysin lytic mechanism controlled by inducible araC/pBAD promoter</td>
 +
                                            <td>Natalia</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387070>BBa_K2387070</a></td>
 +
                                            <td>Repressible aiiA</td>
 +
                                            <td>Natalia</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387071>BBa_K2387071</a></td>
 +
                                            <td>Constitutive TEV protease</td>
 +
                                            <td>Natalia</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387072>BBa_K2387072</a></td>
 +
                                            <td>GFP quenched by REACh2 </td>
 +
                                            <td>Natalia</td>
 +
                                        </tr>
 +
                                        <tr>
 +
                                            <td><a href=http://parts.igem.org/Part:BBa_K2387073>BBa_K2387073</a></td>
 +
                                            <td>Inducible GFP quenched by REACh2 </td>
 +
                                            <td>Natalia</td>
 +
                                        </tr>
 +
                                    </table>
 +
                                </div>
 +
                           
 +
                       
 +
 +
                   
 +
</div>
 +
</div>
 +
 +
<li>We <a href="https://2017.igem.org/Team:Wageningen_UR/Collaborations">collaborated</a> with the Utrecht and TU Delft iGEM teams to verify parts of our systems.</li>
 +
<li>Organized and hosted the BeNeLux iGEM <a href="https://2017.igem.org/Team:Wageningen_UR/HP/Meetup">meet-up</a>. </li>
 +
<li>Participated and completed a RIVM project on <a href="https://2017.igem.org/Team:Wageningen_UR/HP/Comic">biosafety</a>, which was rewarded with a grant.</li>
 +
<li>We thoroughly looked into the <a href="https://2017.igem.org/Team:Wageningen_UR/HP/Ethics">ethical aspects</a> of implementing a new diagnostic.</li>
 +
<li>Since biosafety is a concern when working with GMO's we looked into options of <a href="https://2017.igem.org/Team:Wageningen_UR/Safety">biocontainment</a> and did a literature research on risk and safety assessment.</li>
 +
 +
 +
</ul>
 +
 +
<br>
 +
 
<div class="figure-fullwidth">                    
 
<div class="figure-fullwidth">                    
 
                <img class="figure-fullwidth-img" src="https://static.igem.org/mediawiki/2017/9/94/Bronze_medal_wur.png" style="width: 30%"/>
 
                <img class="figure-fullwidth-img" src="https://static.igem.org/mediawiki/2017/9/94/Bronze_medal_wur.png" style="width: 30%"/>
Line 116: Line 341:
 
   
 
   
 
</div>
 
</div>
−
<ul>
+
<br>
 +
<ul style="border:10px; border-style:solid; border-color:#8b6323; padding: 1em; border-radius: 10px;">
 
<li>Registered for iGEM, had a great summer, and will attend the Giant Jamboree. </li>
 
<li>Registered for iGEM, had a great summer, and will attend the Giant Jamboree. </li>
−
<li>We met all deliverables requirements and submitted XX new <a href="https://2017.igem.org/Team:Wageningen_UR/Parts">Biobricks</a></li>
+
<li>We met all deliverables requirements and submitted 36 new <a href="https://2017.igem.org/Team:Wageningen_UR/Parts">BioBricks</a>.</li>
−
<li>Created an <a href="https://2017.igem.org/Team:Wageningen_UR/Attributions">attributions</a> page with clear attribution of each aspect of our project. </li>
+
<li>Created an <a href="https://2017.igem.org/Team:Wageningen_UR/Attributions">Attributions</a> page with clear attribution of each aspect of our project. </li>
−
<li>We participated in the  <a href="https://2017.igem.org/Team:Wageningen_UR/InterLab">Interlab Measurement Study</a></li>
+
<li>We participated in the  <a href="https://2017.igem.org/Team:Wageningen_UR/InterLab">InterLab Measurement Study</a>.</li>
 
</ul>
 
</ul>
 
 

Latest revision as of 22:56, 1 November 2017

Achievements

This year's Wageningen iGEM team has been working hard and while doing so has kept the medal requirements in mind. Below each medal, a list can be found which completes the criteria that have to be met to earn the medals.


  • Advanced models were created and used to get insight into the quorum sensing and reporter system parts of our project.
  • Integrated Human Practices:
    • We consulted with experts from the Dutch National Institute of Public Health and the Environment (RIVM), the corporate Foundation for Innovative New Diagnostics in Belgium, workers of the Médecines sans Frontières (MSF/Doctors without Borders) organization, a philosophy professor and many more. With the insights gained from experts in the field of diagnostics and people from MSF in Africa and Brazil we were able to improve the diagnostic device to suit the requirements of such a device. We were also able to take the biosafety aspect of our project into account and even consider the ethical dillemma's that arise when implementing a new diagnostic device.
  • We improved BioBricks BBa_I715022 & BBa_I715023 by changing the incorrect split site AA155 to the correct split site AA168 BBa_K2387055.
  • During our project we demonstrated that Mantis is viable in blood serum dilutions, and that fluorescence can indeed be measured in blood serum. Furthermore, we performed experiments to consistently air-dry our cells, and we combined several wet-lab project to perfect the functionality of Mantis.


  • Validated the functionality of the following submitted BioBricks:
    • T. brucei antigens
    BioBrick Code Part Name Designer
    BBa_K2387060 Double tagged ISG64 of T. brucei gambiense Linda
    BBa_K2387061 Double tagged ISG65 of T. brucei gambiense Linda
    Cpx System
    BioBrick Code Part Name Designer
    BBa_K2387014 Cpx system reporter + CpxP inhibitor Stijn
    BBa_K2387017 Cpx system reporter + CpxP-Affinity molecule inhibitor Stijn
    BBa_K2387018 Cpx system reporter + Affinity molecule-CpxP inhibitor Stijn
    BBa_K2387022 Cpx system reporter + tethered CpxP Stijn
    BBa_K2387023 Cpx system reporter + tethered CpxP-Affinity molecule Stijn
    BBa_K2387024 Cpx system reporter + tethered Affinity molecule-CpxP Stijn
    Reporters and Split Reporters
    BioBrick Code Part Name Designer
    BBa_K2387001 Monomeric chromoprotein anm2CP controlled by inducible araC/pBAD promoter José Manuel
    BBa_K2387045 mVenus controlled by inducible araC/pBAD promoter José Manuel
    BBa_K2387046 Split mVenus controlled by inducible araC/pBAD promoter José Manuel
    BBa_K2387047 sfGFP controlled by inducible araC/pBAD promoter José Manuel
    BBa_K2387048 Split sfGFP controlled by inducible araC/pBAD promoter José Manuel
    BBa_K2387052 mCerulean controlled by inducible araC/pBAD promoter José Manuel
    BBa_K2387053 Split mCerulean controlled by inducible araC/pBAD promoter José Manuel
    BBa_K2387054 mRFP controlled by inducible araC/pBAD promoter José Manuel
    BBa_K2387055 Split mRFP controlled by inducible araC/pBAD promoter José Manuel
    BBa_K2387003 eYFP controlled by inducible araC/pBAD promoter Bart
    BBa_K2387007 eYFPn [1-154] controlled by inducible araC/pBAD promoter Bart
    BBa_K2387008 eYFPc [155-238] controlled by inducible araC/pBAD promoter Bart
    BBa_K2387009 eYFPn [1-154] and eYFPc[155-238] controlled by inducible araC/pBAD promoter Bart
    BBa_K2387062 eYFP[155-238]-cLeucine Zipper fusion controlled by inducible araC/pBAD promoter Bart
    BBa_K2387063 eYFP[1-154]-nLeucine Zipper fusion controlled by inducible araC/pBAD promoter Bart
    BBa_K2387065 eYFP[1-154]-nLeucine Zipper and eYFP[155-238]-cLeucine Zipper fusions controlled by inducible araC/pBAD promoter Bart
    Cpx + BiFC
    BioBrick Code Part Name Designer
    BBa_K2387010 CpxA-eYFPn fusion controlled by inducible araC/pBAD promoter Bart
    BBa_K2387029 CpxR-mVenusn and CpxR-mVenusc fusions controlled by inducible araC/pBAD promoter Bart
    BBa_K2387030 CpxR-eYFPc fusion controlled by inducible araC/pBAD promoter Bart
    BBa_K2387031 CpxR-eYFPn fusion controlled by inducible araC/pBAD promoter Bart
    BBa_K2387032 CpxR-eYFPn and CpxR-eYPFc fusions controlled by inducible araC/pBAD promoter Bart
    BBa_K2387074 TEV protease fused to CpxR controlled by inducible araC/pBAD promoter Bart
    Quorum Sensing System
    BioBrick Code Part Name Designer
    BBa_K2387066 Colicin E7 lytic mechanism controlled by inducible araC/pBAD promoter Natalia
    BBa_K2387068 T4 Holin lytic mechanism controlled by inducible araC/pBAD promoter Natalia
    BBa_K2387069 T4 Endolysin lytic mechanism controlled by inducible araC/pBAD promoter Natalia
    BBa_K2387070 Repressible aiiA Natalia
    BBa_K2387071 Constitutive TEV protease Natalia
    BBa_K2387072 GFP quenched by REACh2 Natalia
    BBa_K2387073 Inducible GFP quenched by REACh2 Natalia
  • We collaborated with the Utrecht and TU Delft iGEM teams to verify parts of our systems.
  • Organized and hosted the BeNeLux iGEM meet-up.
  • Participated and completed a RIVM project on biosafety, which was rewarded with a grant.
  • We thoroughly looked into the ethical aspects of implementing a new diagnostic.
  • Since biosafety is a concern when working with GMO's we looked into options of biocontainment and did a literature research on risk and safety assessment.


  • Registered for iGEM, had a great summer, and will attend the Giant Jamboree.
  • We met all deliverables requirements and submitted 36 new BioBricks.
  • Created an Attributions page with clear attribution of each aspect of our project.
  • We participated in the InterLab Measurement Study.