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<h1 style="color:#1AA55E; font-family: Comic sans MS;"> Improve </h1> | <h1 style="color:#1AA55E; font-family: Comic sans MS;"> Improve </h1> | ||
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<b>Bronze medal contribution</b> | <b>Bronze medal contribution</b> | ||
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+ | We improved Part Part:BBa_K145201 (INPUT TetR generator). Initially we designed an expression construct, composed by parts BBa_K145201 and BBa_K081012(GFP Generator). When we expressed this construct in the high copy number vector pSB1C3, we found that a significant basal level of GFP expression can be detected without adding an inducer. This is present on the picture given below (left tube - no induction, right tube - induction with tetracycline).<br /><br /> | ||
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+ | <a href="https://imgur.com/3shbtjA"><img src="https://i.imgur.com/3shbtjA.jpg" title="source: imgur.com" /></a> | ||
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</p> | </p> | ||
Revision as of 23:47, 1 November 2017
Improve
Bronze medal contribution
We improved Part Part:BBa_K145201 (INPUT TetR generator). Initially we designed an expression construct, composed by parts BBa_K145201 and BBa_K081012(GFP Generator). When we expressed this construct in the high copy number vector pSB1C3, we found that a significant basal level of GFP expression can be detected without adding an inducer. This is present on the picture given below (left tube - no induction, right tube - induction with tetracycline).
Gold Medal Criterion #2
Standard Tracks: Improve the function of an existing BioBrick Part. The original part must NOT be from your 2017 part number range. If you change the original part sequence, you must submit a new part. In addition, both the new and original part pages must reference each other. This working part must be different from the part documented in bronze #4 and silver #1.
Special Tracks: Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.