Difference between revisions of "Team:UCSC/Safety"
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| − | <p><span class="firstword">When</span> choosing an organism to help us produce acetaminophen and vitamin B<sub>12</sub> we sought out an FDA-approved microbe; however, we resorted to using a non-toxic cyanobacteria as a model for <i>Arthrospira platensis</i>. <i>A. platensis</i> is generally recognized as safe organism and our genes were chosen from bio-safety level 1 (BSL-1) organisms.</p> | + | <p><span class="firstword">When</span> choosing an organism to help us produce acetaminophen and vitamin B<sub>12</sub> we sought out an FDA-approved microbe; however, we resorted to using a non-toxic cyanobacteria, <i>Synechococcus elongatus</i> strain PCC 7942, as a model for <i>Arthrospira platensis</i>. <i>A. platensis</i> is a generally recognized as safe organism and our genes were chosen from bio-safety level 1 (BSL-1) organisms.</p> |
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| − | <p>We are growing two different strains of <i>S. elongatus</i>: 7002 and 7942. While PCC 7942 will act as our host organism due to its metabolic similarity to <i>A. platensis</i>, we plan to use PCC 7002, which requires external B<sub>12</sub> for survival, as a growth reference for the detection of vitamin B<sub>12</sub>. PCC 7942 was obtained from the University of California, San Diego (UCSD) iGEM team and PCC 7002 was obtained from Dr. Donald Bryant at Pennsylvania State University.</p> | + | <!--<p>We are growing two different strains of <i>S. elongatus</i>: 7002 and 7942. While PCC 7942 will act as our host organism due to its metabolic similarity to <i>A. platensis</i>, we plan to use PCC 7002, which requires external B<sub>12</sub> for survival, as a growth reference for the detection of vitamin B<sub>12</sub>. PCC 7942 was obtained from the University of California, San Diego (UCSD) iGEM team and PCC 7002 was obtained from Dr. Donald Bryant at Pennsylvania State University.</p>--> |
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<h3>VITAMIN B<sub>12</sub></h3> | <h3>VITAMIN B<sub>12</sub></h3> | ||
Latest revision as of 23:58, 1 November 2017
SAFETY
When choosing an organism to help us produce acetaminophen and vitamin B12 we sought out an FDA-approved microbe; however, we resorted to using a non-toxic cyanobacteria, Synechococcus elongatus strain PCC 7942, as a model for Arthrospira platensis. A. platensis is a generally recognized as safe organism and our genes were chosen from bio-safety level 1 (BSL-1) organisms.
L A B SAFETY
Proper PPE for BSL-1 organisms are worn in our laboratory. Within the lab space, every person is expected to be wearing gloves, long pants, close-toed shoes, a lab coat, and protective goggles. All personnel also must be accompanied with one other person so that there is no one alone in lab.
P R O J E C T SAFETY
VITAMIN B12
G E N E S U S E D:
I. ssuE --> produces FMN reductase
Organism: Synechoccocous elongatus strain PCC 7002
Biosafety Level I
The synthesis of 5,6-DMB first requires the presence of reduced flavin mononucleotide (FMNH2). FMNH2 is reduced from flavin mononucleotide (FMN) by FMN reductase, which is encoded with the ssuE gene[19,20].
FMNH2 + NADP+ = FMN + NADPH
II. bluB --> 5,6-DMB synthase
Organism: Sinorhizobium meliloti 1021
Biosafety Level I
ACETAMINOPHEN
G E N E S U S E D:
I. 4ABH --> 4-aminophenol
Organism: Agaricus bisporus
Biosafety Level I
II. nhoA --> converts 4-aminophenol into acetaminophen
Organism: Escherichia coli
Biosafety Level I
