Difference between revisions of "Team:UCopenhagen/HP/Events"

 
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                    <p class="lead"> As mentioned previously, our goal was to actively engage the scientific community and the general public to assure that our project is safe, responsible and good for the world. This was among other things made possible through public presentations and active dialogues at events & conferences. A big applause to everyone that made these events possible!<br><br>
  
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You can find a more detailed overview over all the events or conferences that we have organised and participated in below:
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                    <h2 class="section-heading">Introduction </h2>
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Something, something.
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We've hosted many events to engage the public and experts.
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Here we will also have links pointing down to the events. Will be very nice.
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                    <h2 class="section-heading">Nordic iGEM Conference</h2>
 
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The Nordic iGEM Conference (NiC) is an annual meetup for all nordic teams from Norway, Sweden, Finland and Denmark. The 9 -11th of June inCell hosted the conference at the Department of Plant and Environmental Sciences. It is an traditional to
 
  
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The traditional iGEM presentation competition -. Besides from the mini-jamboree the conference
 
  
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Saturday night the gala dinner gave the participants a great opportunity to bond and strengthen the bond between the nordic iGEM teams.
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<h2 class="section-heading">Nordic iGEM Conference </h2>
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NERMEENS AWESOME NiC VIDEO
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The Nordic iGEM Conference (NiC) is an annual meetup for all nordic teams from Norway, Sweden, Finland and Denmark. <br><br>The 9 -11th of June Incell hosted the conference at the Department of Plant and Environmental Sciences,  in which enriching workshops were held along with a mini-Jamboree to gather feedback on each other’s projects.
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<br><br>On the last night, a gala dinner was organised as a final opportunity for the different Nordic iGEM teams to bond.
  
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<figcaption><b>Image 1 </b>Will Wright at Nordic iGEM Conference (June, 2017).</figcaption>
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                <h2>Scientific approach description</h2>
 
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<p>For both the Interdependence and Number Control projects we have chosen to use Escherichia coli as our chassis. As for the Endocytosis, multiple bacteria spanning different taxonomical groups will be examined. In the following paragraphs, a concise experimental plan for each of the projects will be described.</p>
 
  
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<p>The goal of the interdependence project is to explore the use of amino acid auxotrophy as the framework for a mutually beneficial relationship between two different organisms, namely engineering bacteria to export essential nutrients in a readily available form and in the proper quantity to be sufficient for the host. This will create a strong dependency in a host lacking the production facility of these nutrients. We will perform the experiments on free-living cells, and investigate if the exported amino acids would be sufficient to sustain a S. cerevisiae host. </p>
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<h2 class="section-heading">Lecture Session: Synthetic Biology</h2>
  
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<p> As part of the interdependence project the following will be examined:</p>
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<p class="lead">Synapse - Life Science Connect is a non-profit student organization based in Copenhagen. with focus on bridging the gap between student and the life science industry. <br><br>
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<li> the functional expression of amino acid exporters in E. coli; </li>
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<li>the level of amino acid produced and exported by the symbiont;</li>
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<li> the effect of nutrient export on E. coli cell viability and growth; </li>
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<li> Grow auxotrophic S. cerevisiae in co-culture with respective amino acid exporting E. coli. </li>
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<li>In silico modelling growth of S. cerevisiae under the levels of amino acids that can be exported by E. coli to investigate the minimum number of E. coli symbionts to sustain an auxotrophic yeast host cell.</li>
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On the 27th of September, in collaboration with Synapse, inCell hosted a lecture session on synthetic biology at The Maersk Tower in Copenhagen with the attendance of Associate Professors Rasmus John Normand Frandsen and Christopher Workman from the Technical University of Denmark (DTU), co-founder and CSO of Biosyntia Hans Gene and DTU BioBuilders 2017. <br><br>
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<h3>Number control</h3>
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<p>The goal of the number control project is to lower or stop symbiont replication in case of a high symbiont abundance and/or a starvation status of the host cell, with proof of concept being performed in E. coli cultures. To intertwine the cell replication cycle and lower the stress created by the presence of a symbiont inside the cytoplasm of the host, we aim to put the number control system under control with three signals: the symbiont abundance (a quorum sensing circuit), the host cell starvation status, and the host cell replication. QS and cell starvation should lower symbiont replication, while host replication should increase it.
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The event introduced the participants to the area of synthetic biology from both an academic and industrial perspective. Moreover, a presentation of the framework of the iGEM competition, its past and future was given, as an introduction to the project presentations of DTU BioBuilders 2017 and Incell.
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We aim to establish a replication control and intertwinement using a modular system based on CRISPR/Cas9 technology. A catalytically-dead Cas9 (dCas9) lacking endonuclease activity and a small guide RNA will be guided via RNA-DNA interaction to the origin of replication on the bacterial chromosome, to efficiently and transiently inhibit the chromosome replication (Wiktor J. et al, 2016).</p>
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<p>The dCas9 system will be put under control of the quorum sensing pathway with the lux promoter, which will be active during high symbiont density.
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Additionally, we aim to put the number control system under control of cell starvation status and host cell replication. Moreover, to overcome an unrestrained cell growth, we aim to inhibit the membrane production silencing the expression of a key enzyme for lipid biosynthesis, i.e. Enoyl Acyl Reductase, commonly target of bacteriostatic drugs.</p>
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<p> As part of the Number Control project the following will be examined:</p>
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<li> The effect of dCas9 expression on the cell cycle of E. coli </li>
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<li>The effect of sgRNA binding to various site of the chromosomal origin of replication  on the cell cycle of E. coli</li>
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<li>The effectiveness of the two parts, i.e. dCas9 and sgRNA, to block DNA replication and cell growth </li>
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<li> QS efficiency in controlling dCas9 expression</li>
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<li>Inhibition of Enoyl Acyl Reductase expression via dCas9 in combination with a sgRNA targeting the enzyme promoter.</li>
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<p>The integration of the host signals, i.e. the host starvation status and the cell cycle phase, to control the dCas9 expression will be tested using an in-silico model.</p>
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<figcaption><b>Image 2 </b>Synapse evening with Incell and other students.. </figcaption>
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<p>The goal of the protein import project is to import fluorescent proteins into a bacteria by covalently connecting the protein to a cell-penetrating peptide (CPP).</p>
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<p>CPPs are small peptides (down to 8 amino acids), generally rich in arginine molecules, capable of initiating cellular uptake of a large variety of molecules and proteins by inducing endocytosis. In both plant and mammalian cells CPPs have been shown to mediate protein uptake by both covalent and non-covalent association, with increased specificity during covalent association. However, in bacteria only non-covalent uptake of proteins have so far been demonstrated (Chang et al. 2014). We will explore the utilization of the synthetic CPP nona-arginine (R9) as a vector for facilitation of targeted protein import in bacteria.</p>
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<p> As part of the Endocytosis project the following will be examined: </p>
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<h2 class="section-heading">Vartovs Videnskab - Ethics and Future of Synthetic Biology</h2>
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<li> An initial screening process aimed at evaluating the ability of a variety of bacterial species to take up fluorescent proteins through covalent and non-covalent CPP association </li>
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<p class="lead">Vartovs Videnskab (VV) is a small organization driven by Jamila Lilja and Anna Øhlenschlæger, that arranges evening events on scientific topics for a diverse group of young, old, science nerds, curious minds and entrepreneurs. <br><br>
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<li>If proteins taken up are localized in membrane derived vesicles we will further investigate the potential ability of last 20 amino acids of influenza virus hemagglutinin (H2) to facilitate vesicular escape. </li>
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The 21th of September inCell and Vartovs Videnskab hosted an evening in the sign of synthetic biology for more than 130 individuals.. First, a presentation to Incell’s project was given by team members Jon and Sissel. Subsequently, an insight into synthetic biology’s “green” future was given by Postdoc in plant biochemistry at the University of Copenhagen Thomas Laursen. Thereafter, associate Professor in Philosophy at the University of Copenhagen Sune Holm shared his thoughts on the ethical considerations related to synthetic biology and addressed the need of public discussion of the future of synthetic biology.<br><br>
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<p>Proteins needed for this sub-project will be expressed with His-tags in E. coli and purified using affinity chromatography. Both flow cytometry and confocal microscopy will be used to evaluate bacterial protein uptake through use of the fluorescent proteins YFP and BFP as previously described (Chang et al. 2014). A lipophillic dye capable of staining membrane-derived vesicles (e.g. FM4-64) will be used to investigate cellular localization. To facilitate cleavage of R9 following import, either an endogenous or heterologously expressed peptidase will be used. Successful cleavage will be assessed through use of proteomics (e.g. targeted proteomics). The exact details for evaluation of enzymatic ability following protein import have still not been elucidated, we are however currently contemplating using an enzyme whose activity can be monitored through a colorimetric assay (e.g. 𝜷-lactamase).</p>
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A panel discussion gave the participants the opportunity to directly raise questions on the future of synthetic biology in regards to, among other things,artificial intelligence, robotics, IP rights and the public GMO debate in Denmark.
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<figcaption><b>Image 3 </b>Incell at Vartovs Videnskab with Postdoc Thomas Laursen and Sune Holm. </figcaption>
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<h2 class="section-heading">Ungdommens Naturvidenskabelige Forening (UNF) </h2>
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Spreading the knowledge of the synthetic biology is an very important mission for the inCell team. We think that it is particularly important to communicate and discuss synthetic biology with the younger generations and future scientists/practitioners of science. <br><br>
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Therefore, we teamed up with the former iGEM team of University of Copenhagen, Cosmocrops, to communicate the topic of synthetic biology and our respectice iGEM projects to Ungdommens Naturvidenskabelige Forening (UNF) - the association of science for youth, ranging from high school to university.
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<figcaption><b>Image 4 </b>Incell and Cosmocrops at UNF.</figcaption>
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<li> Archibald, J. M. Endosymbiosis and Eukaryotic Cell Evolution. Current Biology 2015, 25: 911–921. </li>
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<li>Diane Gershon Technology: pushing the boundaries of scientific discovery. Nature Medicine 2003; 9:97</li>
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<li>Jakub Wiktor, Christian Lesterlin, David J. Sherratt, Cees Dekker; CRISPR-mediated control of the bacterial initiation of replication. Nucleic Acids Res 2016; 44 (8): 3801-3810.</li>
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<p class="lead">The 30th of August, the 2017  iGEM Team representing the Southern University of Denmark hosted a workshop focusing on the ethical aspects of synthetic biology and good practices for the coding of the wiki. <br><br> This event inspired many insights that we subsequently integrated into our project.
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<li>Microsugar Chang, Yue-Wern Huang, Robert S. Aronstam and Han-Jung Lee; Cellular Delivery of Noncovalently-Associated Macromolecules by Cell-Penetrating Peptides. Current Pharmaceutical Biotechnology 2014; 15: 267-275. </li>
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                     <h2>Find Incell here:</h2>
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Latest revision as of 00:00, 2 November 2017

E V E N T S


As mentioned previously, our goal was to actively engage the scientific community and the general public to assure that our project is safe, responsible and good for the world. This was among other things made possible through public presentations and active dialogues at events & conferences. A big applause to everyone that made these events possible!

You can find a more detailed overview over all the events or conferences that we have organised and participated in below:

Nordic iGEM Conference

The Nordic iGEM Conference (NiC) is an annual meetup for all nordic teams from Norway, Sweden, Finland and Denmark.

The 9 -11th of June Incell hosted the conference at the Department of Plant and Environmental Sciences, in which enriching workshops were held along with a mini-Jamboree to gather feedback on each other’s projects.

On the last night, a gala dinner was organised as a final opportunity for the different Nordic iGEM teams to bond.

Image 1 Will Wright at Nordic iGEM Conference (June, 2017).

Lecture Session: Synthetic Biology

Synapse - Life Science Connect is a non-profit student organization based in Copenhagen. with focus on bridging the gap between student and the life science industry.

On the 27th of September, in collaboration with Synapse, inCell hosted a lecture session on synthetic biology at The Maersk Tower in Copenhagen with the attendance of Associate Professors Rasmus John Normand Frandsen and Christopher Workman from the Technical University of Denmark (DTU), co-founder and CSO of Biosyntia Hans Gene and DTU BioBuilders 2017.

The event introduced the participants to the area of synthetic biology from both an academic and industrial perspective. Moreover, a presentation of the framework of the iGEM competition, its past and future was given, as an introduction to the project presentations of DTU BioBuilders 2017 and Incell.






Image 2 Synapse evening with Incell and other students..

Vartovs Videnskab - Ethics and Future of Synthetic Biology

Vartovs Videnskab (VV) is a small organization driven by Jamila Lilja and Anna Øhlenschlæger, that arranges evening events on scientific topics for a diverse group of young, old, science nerds, curious minds and entrepreneurs.

The 21th of September inCell and Vartovs Videnskab hosted an evening in the sign of synthetic biology for more than 130 individuals.. First, a presentation to Incell’s project was given by team members Jon and Sissel. Subsequently, an insight into synthetic biology’s “green” future was given by Postdoc in plant biochemistry at the University of Copenhagen Thomas Laursen. Thereafter, associate Professor in Philosophy at the University of Copenhagen Sune Holm shared his thoughts on the ethical considerations related to synthetic biology and addressed the need of public discussion of the future of synthetic biology.

A panel discussion gave the participants the opportunity to directly raise questions on the future of synthetic biology in regards to, among other things,artificial intelligence, robotics, IP rights and the public GMO debate in Denmark.





Image 3 Incell at Vartovs Videnskab with Postdoc Thomas Laursen and Sune Holm.

Ungdommens Naturvidenskabelige Forening (UNF)

Spreading the knowledge of the synthetic biology is an very important mission for the inCell team. We think that it is particularly important to communicate and discuss synthetic biology with the younger generations and future scientists/practitioners of science.

Therefore, we teamed up with the former iGEM team of University of Copenhagen, Cosmocrops, to communicate the topic of synthetic biology and our respectice iGEM projects to Ungdommens Naturvidenskabelige Forening (UNF) - the association of science for youth, ranging from high school to university.

Image 4 Incell and Cosmocrops at UNF.

SDU Wiki writing workshop

The 30th of August, the 2017 iGEM Team representing the Southern University of Denmark hosted a workshop focusing on the ethical aspects of synthetic biology and good practices for the coding of the wiki.

This event inspired many insights that we subsequently integrated into our project.

Image 5 DTU BioBuilders, SDU iGEM and Incell

Find Incell here: