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<meta name="viewport" content="width=device-width, initial-scale=1"> | <meta name="viewport" content="width=device-width, initial-scale=1"> | ||
<link rel="stylesheet" href="main.css"> | <link rel="stylesheet" href="main.css"> | ||
− | |||
<style> | <style> | ||
− | body,h1,h2,h3,h4,h5 {font-family: " | + | body,h1,h2,h3,h4,h5 {font-family: "Arial", sans-serif} |
body {font-size:16px;} | body {font-size:16px;} | ||
.w3-half img{margin-bottom:-6px;margin-top:16px;opacity:0.8;cursor:pointer} | .w3-half img{margin-bottom:-6px;margin-top:16px;opacity:0.8;cursor:pointer} | ||
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</style> | </style> | ||
<body> | <body> | ||
+ | |||
+ | <div id="loader-bg"> | ||
+ | <div id="loader"> | ||
+ | <div class="photo-show"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/f/f4/T--TokyoTech--load_1.png" width="600"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/4/43/T--TokyoTech--load_2.png" width="600"> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
<!-- Sidebar/menu --> | <!-- Sidebar/menu --> | ||
<nav class="w3-sidebar w3-red w3-collapse w3-top w3-large w3-padding" style="z-index:3;width:300px;font-weight:bold;" id="mySidebar"><br> | <nav class="w3-sidebar w3-red w3-collapse w3-top w3-large w3-padding" style="z-index:3;width:300px;font-weight:bold;" id="mySidebar"><br> | ||
<a href="javascript:void(0)" onclick="w3_close()" class="w3-button w3-hide-large w3-display-topleft" style="width:100%;font-size:20px">Close Menu</a> | <a href="javascript:void(0)" onclick="w3_close()" class="w3-button w3-hide-large w3-display-topleft" style="width:100%;font-size:20px">Close Menu</a> | ||
− | <div class="w3-container" style="margin-top: 20px;margin-bottom: 25px"> | + | <div class="w3-container" style="margin-top: 20px;margin-bottom: 25px" id="wrap"> |
<img src="https://static.igem.org/mediawiki/2017/a/a8/T--TokyoTech--logo_white_bright_10211603.png" style="width: 100%"> | <img src="https://static.igem.org/mediawiki/2017/a/a8/T--TokyoTech--logo_white_bright_10211603.png" style="width: 100%"> | ||
</div> | </div> | ||
− | <div style="padding-top: 15px; padding-left: 25px;"> | + | <div style="padding-top: 15px; padding-left: 25px; id="wrap"> |
<div id="contents" style="z-index: 10"> | <div id="contents" style="z-index: 10"> | ||
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<label for="vmcb-d"><a>Experiment</a></label> | <label for="vmcb-d"><a>Experiment</a></label> | ||
<ul> | <ul> | ||
− | <li | + | |
+ | <li> | ||
<input type="checkbox" id="vmcb-d1" /> | <input type="checkbox" id="vmcb-d1" /> | ||
− | + | <label for="vmcb-d1"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/Overview" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white" style="text-align: center;">Overview</a></label> | |
+ | </li> | ||
+ | |||
+ | <li style="padding-bottom: 10px; padding-top: 5px"> | ||
+ | <input type="checkbox" id="vmcb-d2" /> | ||
+ | <label for="vmcb-d2"><a style="text-align: center;">Bacteria to <br>Human Cells ▼</a></label> | ||
<ul> | <ul> | ||
<li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraI_Assay" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">TraI Assay</a></li> | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraI_Assay" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">TraI Assay</a></li> | ||
− | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraI_Improvement" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">TraI | + | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraI_Improvement" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">TraI Improvement <br>Assay</a></li> |
− | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraR_Reporter_Assay" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white" >TraR Reporter <br> Assay</a></li> | + | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraR_Reporter_Assay" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white" >TraR Reporter <br>Assay</a></li> |
− | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/Transcriptome_Analysis" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Transcriptome <br> Analysis</a></li> | + | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/Transcriptome_Analysis" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Transcriptome <br>Analysis</a></li> |
<li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/Chimeric_Transcription_Factor" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Chimeric <br> Transcription <br> Factor Assay</a></li> | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/Chimeric_Transcription_Factor" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Chimeric <br> Transcription <br> Factor Assay</a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
− | <li style="padding-bottom: | + | <li style="padding-bottom: 3px"> |
− | <input type="checkbox" id="vmcb- | + | <input type="checkbox" id="vmcb-d3" /> |
− | <label for="vmcb- | + | <label for="vmcb-d3"><a style="text-align: center;">Human Cells to <br>Bacteria ▼</a></label> |
<ul> | <ul> | ||
<li><a href="https://2017.igem.org/Team:TokyoTech/Experiment/AHK4_Assay" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">AHK4 Assay</a></li> | <li><a href="https://2017.igem.org/Team:TokyoTech/Experiment/AHK4_Assay" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">AHK4 Assay</a></li> | ||
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</li> | </li> | ||
− | <li | + | <li> |
− | <input type="checkbox" id="vmcb- | + | <input type="checkbox" id="vmcb-d4" /> |
− | <label for="vmcb- | + | <label for="vmcb-d4"><a href="https://2017.igem.org/Team:TokyoTech/InterLab" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white" style="text-align: center;">InterLab</a></label> |
</li> | </li> | ||
</ul> | </ul> | ||
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<li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/HP" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Overview</a></li> | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/HP" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Overview</a></li> | ||
<li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/HP/Silver" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Silver</a></li> | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/HP/Silver" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Silver</a></li> | ||
− | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/HP/Gold_Integrated" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Integrated | + | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/HP/Gold_Integrated" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Gold (Integrated)</a></li> |
<li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Demonstrate" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Demonstrate</a></li> | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Demonstrate" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Demonstrate</a></li> | ||
<li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Collaborations" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Collaborations</a></li> | <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Collaborations" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Collaborations</a></li> | ||
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<p style="font-size: 16px; text-indent:1em"> | <p style="font-size: 16px; text-indent:1em"> | ||
− | <a href= | + | <a href=https://2015.igem.org/Team:Tokyo_Tech/Experiment/Interlab>2015 Tokyo_Tech InterLab</a>, accessed Augst 8, 2017 |
</p><br> | </p><br> | ||
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<!-- End page content --> | <!-- End page content --> | ||
+ | </div> | ||
+ | |||
+ | <div class="w3-container" id="contact" style="margin-top:20px"> | ||
+ | <p id="pageTop" style="text-align:right"><a href="#wrap"><img src="https://static.igem.org/mediawiki/2017/0/0d/T--TokyoTech--page_top_2.png" style="width:200px"></a></p> | ||
</div> | </div> | ||
<!-- W3.CSS Container --> | <!-- W3.CSS Container --> | ||
− | <div class="w3-light-grey w3-container w3-padding-32" style="margin-top:75px;padding-right:58px"><p class="w3-right"> | + | <div class="w3-light-grey w3-container w3-padding-32" style="margin-top:75px;padding-right:58px"><p class="w3-right"><a href="http://96haji.me/" title="W3.CSS" target="_blank" class="w3-hover-opacity">Hajime Fujita with W3.CSS: All Rights Reserved</a></p></div> |
+ | <script src="http://ajax.googleapis.com/ajax/libs/jquery/1.11.1/jquery.min.js"></script> | ||
<script> | <script> | ||
// Script to open and close sidebar | // Script to open and close sidebar | ||
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document.getElementById("myOverlay").style.display = "none"; | document.getElementById("myOverlay").style.display = "none"; | ||
} | } | ||
− | + | </script> | |
+ | <script> | ||
// Modal Image Gallery | // Modal Image Gallery | ||
function onClick(element) { | function onClick(element) { | ||
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captionText.innerHTML = element.alt; | captionText.innerHTML = element.alt; | ||
} | } | ||
+ | $(function() { | ||
+ | var h = $(window).height(); | ||
+ | |||
+ | $('#wrap').css('display','none'); | ||
+ | $('#loader-bg ,#loader').height(h).css('display','block'); | ||
+ | }); | ||
+ | |||
+ | $(window).load(function () { //全ての読み込みが完了したら実行 | ||
+ | $('#loader-bg').delay(900).fadeOut(800); | ||
+ | $('#loader').delay(600).fadeOut(300); | ||
+ | $('#wrap').css('display', 'block'); | ||
+ | }); | ||
+ | </script> | ||
+ | <script> | ||
+ | //■page topボタン | ||
+ | $(function(){ | ||
+ | var topBtn=$('#pageTop'); | ||
+ | topBtn.hide(); | ||
+ | |||
+ | //◇ボタンの表示設定 | ||
+ | $(window).scroll(function(){ | ||
+ | if($(this).scrollTop()>80){ | ||
+ | //---- 画面を80pxスクロールしたら、ボタンを表示する | ||
+ | topBtn.fadeIn(); | ||
+ | }else{ | ||
+ | //---- 画面が80pxより上なら、ボタンを表示しない | ||
+ | topBtn.fadeOut(); | ||
+ | } | ||
+ | }); | ||
+ | |||
+ | // ◇ボタンをクリックしたら、スクロールして上に戻る | ||
+ | topBtn.click(function(){ | ||
+ | $('body,html').animate({ | ||
+ | scrollTop: 0},500); | ||
+ | return false; | ||
+ | }); | ||
+ | |||
+ | }); | ||
</script> | </script> | ||
</body> | </body> | ||
</html> | </html> |
Latest revision as of 00:17, 2 November 2017
<!DOCTYPE html>
![](https://static.igem.org/mediawiki/2017/f/f4/T--TokyoTech--load_1.png)
![](https://static.igem.org/mediawiki/2017/4/43/T--TokyoTech--load_2.png)
InterLab
Introduction
In iGEM 2017, the InterLab Study is one of the bronze medal criteria. We measured the GFP expression level of the six designated devices, positive control and negative control using the plate reader. It was the second time for our team to join this InterLab Study (The first time in iGEM 2015).
Summary
Our purpose was to investigate the RBS intensity and promoter intensity effects on gene expression by obtaining the fluorescence data of samples and comparing them. We prepared Device1〜Device6, Positive Control and Negative Control as shown below.
![](https://static.igem.org/mediawiki/parts/2/2f/T--TokyoTech--Interlab_Devices.png)
Results
1. We obtained LUDOX correction values.
![](https://static.igem.org/mediawiki/2017/4/4e/T--TokyoTech--LUDOX_interlab.png)
2. We obtained the two Fluorescein Standard Curve (normal and log scale).
![](https://static.igem.org/mediawiki/2017/8/82/T--TokyoTech--Fluorescein_Standard_Curve.png)
![](https://static.igem.org/mediawiki/2017/a/ad/T--TokyoTech--Fluorescein_Standard_Curve%28log_scale%29.png)
3. We obtained the graph bellow. The RFU of GFP / Turbidity was in the following order, Device1>Device4>Device2>Positive Control>Device5>Device3>Device6>Negative Control.
![](https://static.igem.org/mediawiki/2017/f/f3/T--TokyoTech--Fluo--OD600ave.png)
![](https://static.igem.org/mediawiki/2017/a/a5/T--TokyoTech--Fluo--OD600geo.png)
Discussion
Judging from the results, the gene expression is controlled by the intensity of promoter and the intensity of RBS. The promoter intensity of is supposed to be in the following order, J23101>J23106>J23117. The RBS intensity of I13504 is supposed to be higher than that of BCD2.E0040. Moreover, it is considered that the GFP expression level is dependent on individual to some extent.