Difference between revisions of "PASantiago Chile/results"

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<img src="https://static.igem.org/mediawiki/2017/6/68/Resultasess.jpg" style="margin-top: 31px;">
 
<img src="https://static.igem.org/mediawiki/2017/6/68/Resultasess.jpg" style="margin-top: 31px;">
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</p>
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<p class="human">
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Fig 1.- Resuspended IDT synthesized Gblocks
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</p>
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<p class="human">
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With all of that, we proceed to do the ligation of the parts to form each module.To
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prove it, we did a electrophoresis, where the bands had the right molecular weight.
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<br>
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</p>
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<h2 style= "color:black; margin-top: 71px;text-align: center;">
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Work in progress. 1/11/12 </h2>
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<p class="human">
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Therefore, we did the transformation of the E.Coli,inserting both plasmid for then cultivate in plaques.
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</p>
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<h2 style= "color:black; margin-top: 71px;text-align: center;">
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Work in progress. 1/11/12 </h2>
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<p class="human">
  
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With the objective of do the final check, we did another electrophoresis, and again we could see that the bands had the right molecular weight.In conclusion all the experimental process was correctly executed.
  
 
</p>
 
</p>
  
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<h2 style= "color:black; margin-top: 71px;text-align: center;">Work in Progress 2/11/12</h2>
  
 
</section>
 
</section>

Revision as of 00:26, 2 November 2017


Results

First we prove that the sequences were correct and then we sent to synthesize to IDT.
When we received the DNA, it was resuspended correctly, for the posterior double digest of every part. Which also worked well.

Fig 1.- Resuspended IDT synthesized Gblocks

With all of that, we proceed to do the ligation of the parts to form each module.To prove it, we did a electrophoresis, where the bands had the right molecular weight.

Work in progress. 1/11/12

Therefore, we did the transformation of the E.Coli,inserting both plasmid for then cultivate in plaques.

Work in progress. 1/11/12

With the objective of do the final check, we did another electrophoresis, and again we could see that the bands had the right molecular weight.In conclusion all the experimental process was correctly executed.

Work in Progress 2/11/12