Difference between revisions of "Team:SDSZ-China/Safety"

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         <div class="col-sm-10" style="padding-right:5%">
 
         <div class="col-sm-10" style="padding-right:5%">
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            <h1>Safety</h1>
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            <h2>Bacteria we handled</h2>
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            <p>E.coli BL21(DE3) and DH5alpha, both are BSL1 organisms.</p>
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            <p></p>
 +
            <h2>Chemicals we handled</h2>
 +
            <p>We were exposed to mostly nonhazardous chemicals like agarose, LB and SOB agar, and their raw materials
 +
                like NaCl, KCl. We also handle the neurotoxic chemical polyacrylamide to tun the polyacrylamide gel
 +
                electrophoresis, and we did that under a chemical fume hood, with rubber gloves and lab coats on.</p>
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            <p></p>
 +
            <h2>Lab environment </h2>
 +
            <p>There are open bench, clean bench, and a chemical fume hood in our lab. We are well equipped with
 +
                instruments for molecular biology experiments, such as Quantitative Real-time PCR System, Fluorescence
 +
                microscope, Refrigerated Centrifuge, Metal Bath, Air Heating Incubator, etc. And the Lab for
 +
                Microbiology Experiment was totally 40 square meters, with Biohazard Safety Equipment, Horizontal
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                Laminar Flow Cabinet and other necessary instruments. It can be used for carrying out microbiology
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                experiment with bio-safety level of P2.</p>
 +
            <p></p>
 +
            <h2>Safety protocols in lab</h2>
 +
            <p>In our high school, the lab guidelines focus on both instructing teachers to properly teach and students
 +
                to protect their own safety. It demands teachers should emphasize the lab safety rules and teach
 +
                students to proceed according to instructions; it clearly states that labs should remain ventilated when
 +
                harmful volatile chemicals are used during experiment, that fire sources in lab and preparation rooms
 +
                should be kept away from combustible materials, and that the injured should be immediately treated.</p>
  
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                    <div class="column full_size">
 
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                        <h1 id="Safety">Safety</h1>
 
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                        <p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this
 
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                            year's safety requirements & deadlines, and to learn about safe & responsible research in
 
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                            iGEM.</p>
 
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                        <p>On this page of your wiki, you should write about how you are addressing any safety issues in
 
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                            your project. The wiki is a place where you can <strong>go beyond the questions on the
 
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                                safety forms</strong>, and write about whatever safety topics are most interesting in
 
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                            your project. (You do not need to copy your safety forms onto this wiki page.)</p>
 
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                    </div>
 
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                    <div class="column full_size">
 
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                        <h5>Safe Project Design</h5>
 
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                        <p>Does your project include any safety features? Have you made certain decisions about the
 
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                            design to reduce risks? Write about them here! For example:</p>
 
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                        <ul>
 
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                            <li>Choosing a non-pathogenic chassis</li>
 
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                            <li>Choosing parts that will not harm humans / animals / plants</li>
 
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                            <li>Substituting safer materials for dangerous materials in a proof-of-concept experiment
 
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                            </li>
 
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                            <li>Including an "induced lethality" or "kill-switch" device</li>
 
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                        </ul>
 
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                    </div>
 
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                    <div class="column half_size">
 
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                        <h5>Safe Lab Work</h5>
 
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                        <p>What safety procedures do you use every day in the lab? Did you perform any unusual
 
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                            experiments, or face any unusual safety issues? Write about them here!</p>
 
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                    </div>
 
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                    <div class="column half_size">
 
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                        <h5>Safe Shipment</h5>
 
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                        <p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve
 
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                            those problems?</p>
 
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                    </div>
 
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                </div>
 
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                <br>
 
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                <br>
 
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            </div>
 
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            <br>
 
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Revision as of 00:33, 2 November 2017


Project

Safety

Bacteria we handled

E.coli BL21(DE3) and DH5alpha, both are BSL1 organisms.

Chemicals we handled

We were exposed to mostly nonhazardous chemicals like agarose, LB and SOB agar, and their raw materials like NaCl, KCl. We also handle the neurotoxic chemical polyacrylamide to tun the polyacrylamide gel electrophoresis, and we did that under a chemical fume hood, with rubber gloves and lab coats on.

Lab environment

There are open bench, clean bench, and a chemical fume hood in our lab. We are well equipped with instruments for molecular biology experiments, such as Quantitative Real-time PCR System, Fluorescence microscope, Refrigerated Centrifuge, Metal Bath, Air Heating Incubator, etc. And the Lab for Microbiology Experiment was totally 40 square meters, with Biohazard Safety Equipment, Horizontal Laminar Flow Cabinet and other necessary instruments. It can be used for carrying out microbiology experiment with bio-safety level of P2.

Safety protocols in lab

In our high school, the lab guidelines focus on both instructing teachers to properly teach and students to protect their own safety. It demands teachers should emphasize the lab safety rules and teach students to proceed according to instructions; it clearly states that labs should remain ventilated when harmful volatile chemicals are used during experiment, that fire sources in lab and preparation rooms should be kept away from combustible materials, and that the injured should be immediately treated.