Difference between revisions of "Team:MSU-Michigan/Model"

 
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$(function() {
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$('.myDIV').hover( //when you hover over the figure
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$(this).append($("<img id='char' src='https://static.igem.org/mediawiki/2017/0/03/Shewanella_demonstration.png'/>").hide().fadeIn()); //show the character
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<h1> Modeling</h1>
  
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<div class="w3-pale-blue w3-center" style="width:100%">
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<img src="https://static.igem.org/mediawiki/2017/6/66/MSU-Michigan_Bioreactor.png" style="width:50%;max-width:600px">
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<div class="w3-card w3-padding-16">
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<img src="https://static.igem.org/mediawiki/2017/0/08/T--MSU-Michigan--bioreactordiagram.png" style="width:100%;max-width:800px">
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<div class="w3-card w3-padding-16">
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<img src="https://static.igem.org/mediawiki/2017/0/05/MSU-Michigan_bioreactorassembly.png" style="width:100%;max-width:800px">
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</div>
  
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<h1> Modeling</h1>
 
  
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<p>Mathematical models and computer simulations provide a great way to describe the function and operation of BioBrick Parts and Devices. Synthetic Biology is an engineering discipline, and part of engineering is simulation and modeling to determine the behavior of your design before you build it. Designing and simulating can be iterated many times in a computer before moving to the lab. This award is for teams who build a model of their system and use it to inform system design or simulate expected behavior in conjunction with experiments in the wetlab.</p>
 
  
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<p>We modelled the small single cell bioreactors on drawing programs before building many of them.  We made sure that they were cost effective and are reasonable to build.  A per unit cost is presented in full detail in the drop down list below where the final total cost is $7.43.  This does not include a way to measure the current, which would be about another $30.00 depending on which <a href="https://www.arduino.cc/">Arduino board</a> (1,2,3) you purchase and how you set it up.  This would bring the total cost up to $37.43, which is well within reason for a system that can be reused and will detect harmful contaminants in water.  The cost of a drinking water test can range from $50-270.  While these tests can detect more contaminants than our system currently can it would only take more genetically engineered Shewanella in order to match the range of tests at a significantly more affordable price.</p>
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<h3> Gold Medal Criterion #3</h3>
 
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<p>
 
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To complete for the gold medal criterion #3, please describe your work on this page and fill out the description on your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a>. To achieve this medal criterion, you must convince the judges that your team has gained insight into your project from modeling. You may not convince the judges if your model does not have an effect on your project design or implementation.
 
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Please see the <a href="https://2017.igem.org/Judging/Medals"> 2017 Medals Page</a> for more information.
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<h3>Best Model Special Prize</h3>
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<p>
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To compete for the <a href="https://2017.igem.org/Judging/Awards">Best Model prize</a>, please describe your work on this page  and also fill out the description on the <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a>. Please note you can compete for both the gold medal criterion #3 and the best model prize with this page.
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You must also delete the message box on the top of this page to be eligible for the Best Model Prize.
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<br>
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<br>
  
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<div class="column full_size">
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                <div class="w3-container">
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<h5> Inspiration </h5>
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                  <h2>Single-Chamber Bioreactors</h2>
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<p>
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Here are a few examples from previous teams:
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                    <button onclick="myFunction('openclose6')" class="w3-btn w3-block w3-black w3-left-align">Bioreactor Part List (Click on me)</button>
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</p>
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                    <div id="openclose6" class="w3-container w3-hide">                 
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<h5>Materials:</h5>
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<ul class="w3-ul w3-border">
 
<ul>
 
<ul>
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<li><a href="https://2016.igem.org/Team:Manchester/Model">Manchester 2016</a></li>
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200 mL mason jar
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<li><a href="https://2016.igem.org/Team:TU_Delft/Model">TU Delft 2016  </li>
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<a href="https://www.alibaba.com/product-detail/100ml-200ml-300ml-330ml-500ml-Cylinder_60537054451.html?spm=a2700.7724857.main07.22.7454a40ed1HFTb">Find Part</a href>
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<li><a href="https://2014.igem.org/Team:ETH_Zurich/modeling/overview">ETH Zurich 2014</a></li>
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<li>$0.01-$0.04/jar (Bulk discount; MIN = 10,000 units)</li>
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<li><a href="https://2014.igem.org/Team:Waterloo/Math_Book">Waterloo 2014</a></li>
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<li><b>Per unit: $0.04</b></li>
 
</ul>
 
</ul>
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<ul>
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Glass reference electrode housing
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<a href="https://www.avogadro-lab-supply.com/item/7_mm_x_24_inch_Pyrex_Glass_Borosilicate_Tubing/577">Find Part</a href>
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<li>Cost: $2.65/8 tubes</li>
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<li>Per unit: $0.33</li>
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</ul>
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<ul>
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Magnesia sticks (needed to complete housing)
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<a href="http://www.sigmaaldrich.com/catalog/search?term=31408&interface=All&N=0&mode=match%20partialmax&lang=en&region=US&focus=product">Find Part</a href>
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<li>Cost: $43.70</li>
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<li>Makes 500 housings</li>
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<li>Per unit: $0.09</li>
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</ul>
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<ul>
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Potassium Chloride
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<a href="https://www.avantorinc.com/commerce/product.aspx?id=2147508425">Find Part</a href>
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<li><b>$219.34/2.5 kg</b></li>
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<li>1.3 g/housing</li>
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<li>Per unit: $0.01</li>
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</ul>
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<ul>
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Titanium wire
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<a href="https://malinco.com/fishing/order-online/">Find Part</a href>
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<li>15cm/wire</li>
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<li>Per unit: $0.81</li>
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</ul>
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<ul>
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Carbon felt
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<a href="https://www.alfa.com/en/catalog/043200/">Find Part</a href>
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<li>$52.18 per sheet</li>
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<li>30 x 30 cm (6.35mm thick)</li>
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<li>25 x 25 mm/unit</li>
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<li>144 electrodes per sheet</li>
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<li>Per unit: $0.36</li>
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</ul>
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<ul>
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Sterile 18G needles
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<a href="http://www.drugsupplystore.com/search.php?findify_q=bd%20syringe&findify_limit=24&findify_offset=0">Find Part</a href>
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<li>$0.05 ea.</li>
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<li>x 2 per unit</li>
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<li>Per unit: $0.10</li>
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</ul>
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<ul>
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3mL syringe
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<a href="http://www.drugsupplystore.com/search.php?findify_q=bd%20syringe&findify_limit=24&findify_offset=0">Find Part</a href>
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<li>Per unit: $0.04</li>
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</ul>
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<ul>
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Rubber stopper
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<a href="https://www.mcmaster.com/#rubber-stoppers/=1a20pmp">Find Part</a href>
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<li>trade size #12 (2 1/2", tapered to 2 1/8")</li>
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<li>Per unit: $2.31</li>
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</ul>
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<ul>
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Rubber stopper
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<a href="https://www.mcmaster.com/#rubber-stoppers/=1a20pmp">Find Part</a href>
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<li>trade size C (3/8" tapered to 7/32")</li>
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<li>Per unit: $0.71</li>
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</ul>
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<ul>
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Octogonal magnetic stir bars with pivot ring
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<a href="https://www.mcmaster.com/#stir-bars/=1a20t03">Find Part</a href>
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<li>Per stir bar: $3.96</li>
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<li>Bulk unit via MSU chemistry store</li>
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<li>Per unit: $1.60</li>
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</ul>
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<ul>
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Leit-C for microscopy (carbon cement for electrodes)
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<a href="http://www.sigmaaldrich.com/catalog/search?term=09929-30G&interface=All&N=0&mode=match%20partialmax&lang=en&region=US&focus=product">Find Part</a href>
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<li>$48.20</li>
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<li>Makes 75 eletrodes</li>
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<li>Per unit: $0.64</li>
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</ul>
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<ul>
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Xylene
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<a href="https://www.fishersci.com/us/en/catalog/search/products?keyword=xylene&nav=">Find Part</a href>
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<li>Per 500ml: $0.01</li>
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</ul>
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<ul>
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18G Hypodermic sampling needle (4")
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<a href="https://www.fishersci.com/shop/products/air-tite-premium-hypodermic-needles-lab-vet-use-22g-x-4-in/14817102">Find Part</a href>
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<li>Cost: $39.26</li>
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<li>Package has 100 needles</li>
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<li>Per unit: $0.39</li>
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</ul>
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</div>
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</div>
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<br>
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<br>
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<br>
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<br>
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<br>
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<br>
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<br>
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<h1>References</h1>
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<p>
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<br>(1)  Aristizabal, D. H.; Giraldo, D. A.; Sanchez, S.; Taborda, G.; Baeza, A. J. Phys. Conf. Ser. 2017, 365, 11001.
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<br>(2)  Jannelli, N.; Anna Nastro, R.; Cigolotti, V.; Minutillo, M.; Falcucci, G. Appl. Energy 2017, 192, 543–550.
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<br>(3)  Yang, Y.; Ren, H.; Ben-Tzvi, P.; Yang, X.; He, Z. Int. J. Hydrogen Energy 2017, 42 (31), 20260–20268.
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</p>
 
</html>
 
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Latest revision as of 00:39, 2 November 2017

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Applied Design Hardware Measurement Model

Modeling

We modelled the small single cell bioreactors on drawing programs before building many of them. We made sure that they were cost effective and are reasonable to build. A per unit cost is presented in full detail in the drop down list below where the final total cost is $7.43. This does not include a way to measure the current, which would be about another $30.00 depending on which Arduino board (1,2,3) you purchase and how you set it up. This would bring the total cost up to $37.43, which is well within reason for a system that can be reused and will detect harmful contaminants in water. The cost of a drinking water test can range from $50-270. While these tests can detect more contaminants than our system currently can it would only take more genetically engineered Shewanella in order to match the range of tests at a significantly more affordable price.




Single-Chamber Bioreactors

Materials:
      200 mL mason jar Find Part
    • $0.01-$0.04/jar (Bulk discount; MIN = 10,000 units)
    • Per unit: $0.04
      Glass reference electrode housing Find Part
    • Cost: $2.65/8 tubes
    • Per unit: $0.33
      Magnesia sticks (needed to complete housing) Find Part
    • Cost: $43.70
    • Makes 500 housings
    • Per unit: $0.09
      Potassium Chloride Find Part
    • $219.34/2.5 kg
    • 1.3 g/housing
    • Per unit: $0.01
      Titanium wire Find Part
    • 15cm/wire
    • Per unit: $0.81
      Carbon felt Find Part
    • $52.18 per sheet
    • 30 x 30 cm (6.35mm thick)
    • 25 x 25 mm/unit
    • 144 electrodes per sheet
    • Per unit: $0.36
      Sterile 18G needles Find Part
    • $0.05 ea.
    • x 2 per unit
    • Per unit: $0.10
      Rubber stopper Find Part
    • trade size #12 (2 1/2", tapered to 2 1/8")
    • Per unit: $2.31
      Rubber stopper Find Part
    • trade size C (3/8" tapered to 7/32")
    • Per unit: $0.71
      Octogonal magnetic stir bars with pivot ring Find Part
    • Per stir bar: $3.96
    • Bulk unit via MSU chemistry store
    • Per unit: $1.60
      Leit-C for microscopy (carbon cement for electrodes) Find Part
    • $48.20
    • Makes 75 eletrodes
    • Per unit: $0.64
      18G Hypodermic sampling needle (4") Find Part
    • Cost: $39.26
    • Package has 100 needles
    • Per unit: $0.39








References


(1) Aristizabal, D. H.; Giraldo, D. A.; Sanchez, S.; Taborda, G.; Baeza, A. J. Phys. Conf. Ser. 2017, 365, 11001.
(2) Jannelli, N.; Anna Nastro, R.; Cigolotti, V.; Minutillo, M.; Falcucci, G. Appl. Energy 2017, 192, 543–550.
(3) Yang, Y.; Ren, H.; Ben-Tzvi, P.; Yang, X.; He, Z. Int. J. Hydrogen Energy 2017, 42 (31), 20260–20268.

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