Difference between revisions of "Team:MSU-Michigan/Model"

 
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<html>
 
<html>
  
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<script>
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$(function() {
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<div class="column full_size">
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$('.myDIV').hover( //when you hover over the figure
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function() {
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$(this).append($("<img id='char' src='https://static.igem.org/mediawiki/2017/0/03/Shewanella_demonstration.png'/>").hide().fadeIn()); //show the character
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}, function() {
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$('#char').remove(); //get rid of it after moving the cursor away from the figure
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});
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});
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</script>
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<div class="w3-cell-row">
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  <div class="w3-col w3-container" style="width:17.5%"></div>
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  <div class="w3-col w3-container w3-pale-green" style="width:60%">
 
<h1> Modeling</h1>
 
<h1> Modeling</h1>
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<div class="w3-pale-blue w3-center" style="width:100%">
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    <div class="w3-container w3-center">
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<div class="w3-card w3-padding-16">
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<img src="https://static.igem.org/mediawiki/2017/6/66/MSU-Michigan_Bioreactor.png" style="width:50%;max-width:600px">
 
</div>
 
</div>
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<div class="column half_size">
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<div class="w3-card w3-padding-16">
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<img src="https://static.igem.org/mediawiki/2017/c/cd/MSU-Michigan_Bioreactor_setup.jpeg">
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<img src="https://static.igem.org/mediawiki/2017/0/08/T--MSU-Michigan--bioreactordiagram.png" style="width:100%;max-width:800px">
 
</div>
 
</div>
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<div class="clear"></div>
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<div class="w3-card w3-padding-16">
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<img src="https://static.igem.org/mediawiki/2017/0/05/MSU-Michigan_bioreactorassembly.png" style="width:100%;max-width:800px">
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</div>
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</div>
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</div>
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<p>We modelled the small single cell bioreactors on drawing programs before building many of them.  We made sure that they were cost effective and are reasonable to build.  A per unit cost is presented in full detail in the drop down list below where the final total cost is $7.43.  This does not include a way to measure the current, which would be about another $30.00 depending on which <a href="https://www.arduino.cc/">Arduino board</a> (1,2,3) you purchase and how you set it up.  This would bring the total cost up to $37.43, which is well within reason for a system that can be reused and will detect harmful contaminants in water.  The cost of a drinking water test can range from $50-270.  While these tests can detect more contaminants than our system currently can it would only take more genetically engineered Shewanella in order to match the range of tests at a significantly more affordable price.</p>
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<br>
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<br>
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                 <div class="w3-container">
 
                 <div class="w3-container">
 
                   <h2>Single-Chamber Bioreactors</h2>
 
                   <h2>Single-Chamber Bioreactors</h2>
  
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                     <button onclick="myFunction('openclose6')" class="w3-btn w3-block w3-black w3-left-align">Bioreactor Part List</button>
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                     <button onclick="myFunction('openclose6')" class="w3-btn w3-block w3-black w3-left-align">Bioreactor Part List (Click on me)</button>
 
                     <div id="openclose6" class="w3-container w3-hide">                   
 
                     <div id="openclose6" class="w3-container w3-hide">                   
  
 
<h5>Materials:</h5>
 
<h5>Materials:</h5>
 
<ul class="w3-ul w3-border">
 
<ul class="w3-ul w3-border">
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<ul>
 
200 mL mason jar
 
200 mL mason jar
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<a href="https://www.alibaba.com/product-detail/100ml-200ml-300ml-330ml-500ml-Cylinder_60537054451.html?spm=a2700.7724857.main07.22.7454a40ed1HFTb">Find Part</a href>
 
<li>$0.01-$0.04/jar (Bulk discount; MIN = 10,000 units)</li>
 
<li>$0.01-$0.04/jar (Bulk discount; MIN = 10,000 units)</li>
−
    <ul>
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<li><b>Per unit: $0.04</b></li>
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<li><b>$0.04/unit</b></li>
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</ul>
 
</ul>
 
<ul>
 
<ul>
−
Glass reference electrode
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Glass reference electrode housing
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<li>Potassium Chloride, crystal<a href=”https://www.avantorinc.com/commerce/product.aspx?id=2147508425”>purchase </a> <li><b>$219.34/2.5 kg</b></li>
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<a href="https://www.avogadro-lab-supply.com/item/7_mm_x_24_inch_Pyrex_Glass_Borosilicate_Tubing/577">Find Part</a href>
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<li>1.3 g/unit</li>
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<li>Cost: $2.65/8 tubes</li>
−
<li>$0.01/unit</li>
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<li>Per unit: $0.33</li>
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</ul>
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<ul>
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Magnesia sticks (needed to complete housing)
 +
<a href="http://www.sigmaaldrich.com/catalog/search?term=31408&interface=All&N=0&mode=match%20partialmax&lang=en&region=US&focus=product">Find Part</a href>
 +
<li>Cost: $43.70</li>
 +
<li>Makes 500 housings</li>
 +
<li>Per unit: $0.09</li>
 +
</ul>
 +
<ul>
 +
Potassium Chloride
 +
<a href="https://www.avantorinc.com/commerce/product.aspx?id=2147508425">Find Part</a href>
 +
<li><b>$219.34/2.5 kg</b></li>
 +
<li>1.3 g/housing</li>
 +
<li>Per unit: $0.01</li>
 
</ul>
 
</ul>
 
<ul>
 
<ul>
 
Titanium wire
 
Titanium wire
−
<li>15cm/unit = .15 m/unit</li>
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<a href="https://malinco.com/fishing/order-online/">Find Part</a href>
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<li>$0.81/unit</li>
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<li>15cm/wire</li>
 +
<li>Per unit: $0.81</li>
 
</ul>
 
</ul>
 
<ul>
 
<ul>
−
Carbon felt  
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Carbon felt
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<li><b>$52.18 per sheet</b></li>
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<a href="https://www.alfa.com/en/catalog/043200/">Find Part</a href>
−
<ul>
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<li>$52.18 per sheet</li>
 
<li>30 x 30 cm (6.35mm thick)</li>
 
<li>30 x 30 cm (6.35mm thick)</li>
 
<li>25 x 25 mm/unit</li>
 
<li>25 x 25 mm/unit</li>
 
<li>144 electrodes per sheet</li>
 
<li>144 electrodes per sheet</li>
 +
<li>Per unit: $0.36</li>
 
</ul>
 
</ul>
−
</ul>
 
−
<li>$0.36/unit</li>
 
 
<ul>
 
<ul>
 
Sterile 18G needles
 
Sterile 18G needles
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<li><b>$0.05 ea.</b></li>
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<a href="http://www.drugsupplystore.com/search.php?findify_q=bd%20syringe&findify_limit=24&findify_offset=0">Find Part</a href>
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<ul>
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<li>$0.05 ea.</li>
 
<li>x 2 per unit</li>
 
<li>x 2 per unit</li>
−
</ul>
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<li>Per unit: $0.10</li>
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<li>$0.10/unit<li>
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</ul>
 
</ul>
 
<ul>
 
<ul>
 
3mL syringe
 
3mL syringe
−
<li>$0.04/unit</li>
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<a href="http://www.drugsupplystore.com/search.php?findify_q=bd%20syringe&findify_limit=24&findify_offset=0">Find Part</a href>
 +
<li>Per unit: $0.04</li>
 
</ul>
 
</ul>
 
<ul>
 
<ul>
−
Rubber stopper  
+
Rubber stopper
 +
<a href="https://www.mcmaster.com/#rubber-stoppers/=1a20pmp">Find Part</a href>
 
<li>trade size #12 (2 1/2", tapered to 2 1/8")</li>
 
<li>trade size #12 (2 1/2", tapered to 2 1/8")</li>
−
<li>$2.31/unit</li>
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<li>Per unit: $2.31</li>
 
</ul>
 
</ul>
 
<ul>
 
<ul>
 
Rubber stopper  
 
Rubber stopper  
 +
<a href="https://www.mcmaster.com/#rubber-stoppers/=1a20pmp">Find Part</a href>
 
<li>trade size C (3/8" tapered to 7/32")</li>
 
<li>trade size C (3/8" tapered to 7/32")</li>
−
<li>$0.71/unit</li>
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<li>Per unit: $0.71</li>
 
</ul>
 
</ul>
 
<ul>
 
<ul>
 
Octogonal magnetic stir bars with pivot ring
 
Octogonal magnetic stir bars with pivot ring
−
<li><b>$1.60/unit</b></li>
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<a href="https://www.mcmaster.com/#stir-bars/=1a20t03">Find Part</a href>
 +
<li>Per stir bar: $3.96</li>
 +
<li>Bulk unit via MSU chemistry store</li>
 +
<li>Per unit: $1.60</li>
 
</ul>
 
</ul>
 
<ul>
 
<ul>
 
Leit-C for microscopy (carbon cement for electrodes)
 
Leit-C for microscopy (carbon cement for electrodes)
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<a href=”http://www.sigmaaldrich.com/catalog/product/sial/09929?lang=en&region=USSigma-Aldrich”>Sigma Aldrich</a>
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<a href="http://www.sigmaaldrich.com/catalog/search?term=09929-30G&interface=All&N=0&mode=match%20partialmax&lang=en&region=US&focus=product">Find Part</a href>
−
<li>$48.20/30g</li>
+
<li>$48.20</li>
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<li>$0.64/unit</li>
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<li>Makes 75 eletrodes</li>
 +
<li>Per unit: $0.64</li>
 
</ul>
 
</ul>
 
<ul>
 
<ul>
 
Xylene
 
Xylene
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<li>$0.005/500mL</li>
+
<a href="https://www.fishersci.com/us/en/catalog/search/products?keyword=xylene&nav=">Find Part</a href>
 +
<li>Per 500ml: $0.01</li>
 
</ul>
 
</ul>
 
<ul>
 
<ul>
−
18G Hypodermic sampling needle (3")
+
18G Hypodermic sampling needle (4")
−
<a href= “https://www.fishersci.com/shop/products/air-tite-premium-hypodermic-needles-lab-vet-use-6/p-2537820#?keyword=hypodermic+needle”>Fisher Scientific</a>
+
<a href="https://www.fishersci.com/shop/products/air-tite-premium-hypodermic-needles-lab-vet-use-22g-x-4-in/14817102">Find Part</a href>
−
<li>Pack of 100 for $39.26</li>
+
<li>Cost: $39.26</li>
−
<li>$0.39/unit </li>
+
<li>Package has 100 needles</li>
 +
<li>Per unit: $0.39</li>
 
</ul>
 
</ul>
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</div>
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</div>
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<br>
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<br>
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<br>
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<br>
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<br>
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             </script>
 
             </script>
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<br>
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<br>
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<br>
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<h1>References</h1>
 +
<p>
 +
<br>(1)  Aristizabal, D. H.; Giraldo, D. A.; Sanchez, S.; Taborda, G.; Baeza, A. J. Phys. Conf. Ser. 2017, 365, 11001.
 +
<br>(2)  Jannelli, N.; Anna Nastro, R.; Cigolotti, V.; Minutillo, M.; Falcucci, G. Appl. Energy 2017, 192, 543–550.
 +
<br>(3)  Yang, Y.; Ren, H.; Ben-Tzvi, P.; Yang, X.; He, Z. Int. J. Hydrogen Energy 2017, 42 (31), 20260–20268.
 +
</p>
 
</html>
 
</html>
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{{MSU-Michigan-Footer}}

Latest revision as of 00:39, 2 November 2017

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Parts
Applied Design Hardware Measurement Model

Modeling

We modelled the small single cell bioreactors on drawing programs before building many of them. We made sure that they were cost effective and are reasonable to build. A per unit cost is presented in full detail in the drop down list below where the final total cost is $7.43. This does not include a way to measure the current, which would be about another $30.00 depending on which Arduino board (1,2,3) you purchase and how you set it up. This would bring the total cost up to $37.43, which is well within reason for a system that can be reused and will detect harmful contaminants in water. The cost of a drinking water test can range from $50-270. While these tests can detect more contaminants than our system currently can it would only take more genetically engineered Shewanella in order to match the range of tests at a significantly more affordable price.




Single-Chamber Bioreactors

Materials:
      200 mL mason jar Find Part
    • $0.01-$0.04/jar (Bulk discount; MIN = 10,000 units)
    • Per unit: $0.04
      Glass reference electrode housing Find Part
    • Cost: $2.65/8 tubes
    • Per unit: $0.33
      Magnesia sticks (needed to complete housing) Find Part
    • Cost: $43.70
    • Makes 500 housings
    • Per unit: $0.09
      Potassium Chloride Find Part
    • $219.34/2.5 kg
    • 1.3 g/housing
    • Per unit: $0.01
      Titanium wire Find Part
    • 15cm/wire
    • Per unit: $0.81
      Carbon felt Find Part
    • $52.18 per sheet
    • 30 x 30 cm (6.35mm thick)
    • 25 x 25 mm/unit
    • 144 electrodes per sheet
    • Per unit: $0.36
      Sterile 18G needles Find Part
    • $0.05 ea.
    • x 2 per unit
    • Per unit: $0.10
      Rubber stopper Find Part
    • trade size #12 (2 1/2", tapered to 2 1/8")
    • Per unit: $2.31
      Rubber stopper Find Part
    • trade size C (3/8" tapered to 7/32")
    • Per unit: $0.71
      Octogonal magnetic stir bars with pivot ring Find Part
    • Per stir bar: $3.96
    • Bulk unit via MSU chemistry store
    • Per unit: $1.60
      Leit-C for microscopy (carbon cement for electrodes) Find Part
    • $48.20
    • Makes 75 eletrodes
    • Per unit: $0.64
      18G Hypodermic sampling needle (4") Find Part
    • Cost: $39.26
    • Package has 100 needles
    • Per unit: $0.39








References


(1) Aristizabal, D. H.; Giraldo, D. A.; Sanchez, S.; Taborda, G.; Baeza, A. J. Phys. Conf. Ser. 2017, 365, 11001.
(2) Jannelli, N.; Anna Nastro, R.; Cigolotti, V.; Minutillo, M.; Falcucci, G. Appl. Energy 2017, 192, 543–550.
(3) Yang, Y.; Ren, H.; Ben-Tzvi, P.; Yang, X.; He, Z. Int. J. Hydrogen Energy 2017, 42 (31), 20260–20268.

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