Amburn Brian (Talk | contribs) |
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<html> | <html> | ||
− | + | <script> | |
− | + | $(function() { | |
− | <div class=" | + | $('.myDIV').hover( //when you hover over the figure |
+ | function() { | ||
+ | $(this).append($("<img id='char' src='https://static.igem.org/mediawiki/2017/0/03/Shewanella_demonstration.png'/>").hide().fadeIn()); //show the character | ||
+ | }, function() { | ||
+ | $('#char').remove(); //get rid of it after moving the cursor away from the figure | ||
+ | }); | ||
+ | }); | ||
+ | </script> | ||
+ | <div class="w3-cell-row"> | ||
+ | <div class="w3-col w3-container" style="width:17.5%"></div> | ||
+ | <div class="w3-col w3-container w3-pale-green" style="width:60%"> | ||
<h1> Modeling</h1> | <h1> Modeling</h1> | ||
− | + | ||
− | + | <div class="w3-pale-blue w3-center" style="width:100%"> | |
− | <div class="w3- | + | |
<div class="w3-container w3-center"> | <div class="w3-container w3-center"> | ||
− | <img src="https://static.igem.org/mediawiki/2017/6/66/MSU-Michigan_Bioreactor.png" style="width:50%;max-width: | + | <div class="w3-card w3-padding-16"> |
+ | <img src="https://static.igem.org/mediawiki/2017/6/66/MSU-Michigan_Bioreactor.png" style="width:50%;max-width:600px"> | ||
</div> | </div> | ||
+ | <div class="w3-card w3-padding-16"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/0/08/T--MSU-Michigan--bioreactordiagram.png" style="width:100%;max-width:800px"> | ||
</div> | </div> | ||
− | <div class=" | + | <div class="w3-card w3-padding-16"> |
+ | <img src="https://static.igem.org/mediawiki/2017/0/05/MSU-Michigan_bioreactorassembly.png" style="width:100%;max-width:800px"> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | <p>We modelled the small single cell bioreactors on drawing programs before building many of them. We made sure that they were cost effective and are reasonable to build. A per unit cost is presented in full detail in the drop down list below where the final total cost is $7.43. This does not include a way to measure the current, which would be about another $30.00 depending on which <a href="https://www.arduino.cc/">Arduino board</a> (1,2,3) you purchase and how you set it up. This would bring the total cost up to $37.43, which is well within reason for a system that can be reused and will detect harmful contaminants in water. The cost of a drinking water test can range from $50-270. While these tests can detect more contaminants than our system currently can it would only take more genetically engineered Shewanella in order to match the range of tests at a significantly more affordable price.</p> | ||
+ | <br> | ||
+ | |||
<head> | <head> | ||
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</head> | </head> | ||
− | + | ||
+ | <br> | ||
+ | <br> | ||
+ | |||
<div class="w3-container"> | <div class="w3-container"> | ||
<h2>Single-Chamber Bioreactors</h2> | <h2>Single-Chamber Bioreactors</h2> | ||
− | <button onclick="myFunction('openclose6')" class="w3-btn w3-block w3-black w3-left-align">Bioreactor Part List</button> | + | <button onclick="myFunction('openclose6')" class="w3-btn w3-block w3-black w3-left-align">Bioreactor Part List (Click on me)</button> |
<div id="openclose6" class="w3-container w3-hide"> | <div id="openclose6" class="w3-container w3-hide"> | ||
<h5>Materials:</h5> | <h5>Materials:</h5> | ||
<ul class="w3-ul w3-border"> | <ul class="w3-ul w3-border"> | ||
+ | <ul> | ||
200 mL mason jar | 200 mL mason jar | ||
+ | <a href="https://www.alibaba.com/product-detail/100ml-200ml-300ml-330ml-500ml-Cylinder_60537054451.html?spm=a2700.7724857.main07.22.7454a40ed1HFTb">Find Part</a href> | ||
<li>$0.01-$0.04/jar (Bulk discount; MIN = 10,000 units)</li> | <li>$0.01-$0.04/jar (Bulk discount; MIN = 10,000 units)</li> | ||
− | + | <li><b>Per unit: $0.04</b></li> | |
− | <li><b>$0.04 | + | |
</ul> | </ul> | ||
<ul> | <ul> | ||
− | Glass reference electrode | + | Glass reference electrode housing |
− | <li> | + | <a href="https://www.avogadro-lab-supply.com/item/7_mm_x_24_inch_Pyrex_Glass_Borosilicate_Tubing/577">Find Part</a href> |
+ | <li>Cost: $2.65/8 tubes</li> | ||
+ | <li>Per unit: $0.33</li> | ||
+ | </ul> | ||
+ | <ul> | ||
+ | Magnesia sticks (needed to complete housing) | ||
+ | <a href="http://www.sigmaaldrich.com/catalog/search?term=31408&interface=All&N=0&mode=match%20partialmax&lang=en®ion=US&focus=product">Find Part</a href> | ||
+ | <li>Cost: $43.70</li> | ||
+ | <li>Makes 500 housings</li> | ||
+ | <li>Per unit: $0.09</li> | ||
+ | </ul> | ||
+ | <ul> | ||
+ | Potassium Chloride | ||
<a href="https://www.avantorinc.com/commerce/product.aspx?id=2147508425">Find Part</a href> | <a href="https://www.avantorinc.com/commerce/product.aspx?id=2147508425">Find Part</a href> | ||
<li><b>$219.34/2.5 kg</b></li> | <li><b>$219.34/2.5 kg</b></li> | ||
− | <li>1.3 g/ | + | <li>1.3 g/housing</li> |
− | <li>$0.01 | + | <li>Per unit: $0.01</li> |
</ul> | </ul> | ||
<ul> | <ul> | ||
Titanium wire | Titanium wire | ||
− | <li>15cm/ | + | <a href="https://malinco.com/fishing/order-online/">Find Part</a href> |
− | <li>$0.81 | + | <li>15cm/wire</li> |
+ | <li>Per unit: $0.81</li> | ||
</ul> | </ul> | ||
<ul> | <ul> | ||
− | Carbon felt | + | Carbon felt |
− | < | + | <a href="https://www.alfa.com/en/catalog/043200/">Find Part</a href> |
− | + | <li>$52.18 per sheet</li> | |
<li>30 x 30 cm (6.35mm thick)</li> | <li>30 x 30 cm (6.35mm thick)</li> | ||
<li>25 x 25 mm/unit</li> | <li>25 x 25 mm/unit</li> | ||
<li>144 electrodes per sheet</li> | <li>144 electrodes per sheet</li> | ||
+ | <li>Per unit: $0.36</li> | ||
</ul> | </ul> | ||
− | |||
− | |||
<ul> | <ul> | ||
Sterile 18G needles | Sterile 18G needles | ||
− | < | + | <a href="http://www.drugsupplystore.com/search.php?findify_q=bd%20syringe&findify_limit=24&findify_offset=0">Find Part</a href> |
− | + | <li>$0.05 ea.</li> | |
<li>x 2 per unit</li> | <li>x 2 per unit</li> | ||
− | + | <li>Per unit: $0.10</li> | |
− | + | ||
− | <li>$0.10/ | + | |
</ul> | </ul> | ||
<ul> | <ul> | ||
3mL syringe | 3mL syringe | ||
− | <li>$0.04 | + | <a href="http://www.drugsupplystore.com/search.php?findify_q=bd%20syringe&findify_limit=24&findify_offset=0">Find Part</a href> |
+ | <li>Per unit: $0.04</li> | ||
</ul> | </ul> | ||
<ul> | <ul> | ||
− | Rubber stopper | + | Rubber stopper |
+ | <a href="https://www.mcmaster.com/#rubber-stoppers/=1a20pmp">Find Part</a href> | ||
<li>trade size #12 (2 1/2", tapered to 2 1/8")</li> | <li>trade size #12 (2 1/2", tapered to 2 1/8")</li> | ||
− | <li>$2.31 | + | <li>Per unit: $2.31</li> |
</ul> | </ul> | ||
<ul> | <ul> | ||
Rubber stopper | Rubber stopper | ||
+ | <a href="https://www.mcmaster.com/#rubber-stoppers/=1a20pmp">Find Part</a href> | ||
<li>trade size C (3/8" tapered to 7/32")</li> | <li>trade size C (3/8" tapered to 7/32")</li> | ||
− | <li>$0.71 | + | <li>Per unit: $0.71</li> |
</ul> | </ul> | ||
<ul> | <ul> | ||
Octogonal magnetic stir bars with pivot ring | Octogonal magnetic stir bars with pivot ring | ||
− | < | + | <a href="https://www.mcmaster.com/#stir-bars/=1a20t03">Find Part</a href> |
+ | <li>Per stir bar: $3.96</li> | ||
+ | <li>Bulk unit via MSU chemistry store</li> | ||
+ | <li>Per unit: $1.60</li> | ||
</ul> | </ul> | ||
<ul> | <ul> | ||
Leit-C for microscopy (carbon cement for electrodes) | Leit-C for microscopy (carbon cement for electrodes) | ||
− | <a href= | + | <a href="http://www.sigmaaldrich.com/catalog/search?term=09929-30G&interface=All&N=0&mode=match%20partialmax&lang=en®ion=US&focus=product">Find Part</a href> |
− | <li>$48.20/ | + | <li>$48.20</li> |
− | <li>$0.64 | + | <li>Makes 75 eletrodes</li> |
+ | <li>Per unit: $0.64</li> | ||
</ul> | </ul> | ||
<ul> | <ul> | ||
Xylene | Xylene | ||
− | <li>$0. | + | <a href="https://www.fishersci.com/us/en/catalog/search/products?keyword=xylene&nav=">Find Part</a href> |
+ | <li>Per 500ml: $0.01</li> | ||
</ul> | </ul> | ||
<ul> | <ul> | ||
− | 18G Hypodermic sampling needle ( | + | 18G Hypodermic sampling needle (4") |
− | <a href= | + | <a href="https://www.fishersci.com/shop/products/air-tite-premium-hypodermic-needles-lab-vet-use-22g-x-4-in/14817102">Find Part</a href> |
− | <li> | + | <li>Cost: $39.26</li> |
− | <li>$0.39 | + | <li>Package has 100 needles</li> |
+ | <li>Per unit: $0.39</li> | ||
</ul> | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | |||
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} | } | ||
</script> | </script> | ||
− | + | <br> | |
+ | <br> | ||
+ | <br> | ||
+ | <h1>References</h1> | ||
+ | <p> | ||
+ | <br>(1) Aristizabal, D. H.; Giraldo, D. A.; Sanchez, S.; Taborda, G.; Baeza, A. J. Phys. Conf. Ser. 2017, 365, 11001. | ||
+ | <br>(2) Jannelli, N.; Anna Nastro, R.; Cigolotti, V.; Minutillo, M.; Falcucci, G. Appl. Energy 2017, 192, 543–550. | ||
+ | <br>(3) Yang, Y.; Ren, H.; Ben-Tzvi, P.; Yang, X.; He, Z. Int. J. Hydrogen Energy 2017, 42 (31), 20260–20268. | ||
+ | </p> | ||
</html> | </html> | ||
{{MSU-Michigan-Footer}} | {{MSU-Michigan-Footer}} |
Latest revision as of 00:39, 2 November 2017
Modeling
![](https://static.igem.org/mediawiki/2017/6/66/MSU-Michigan_Bioreactor.png)
![](https://static.igem.org/mediawiki/2017/0/08/T--MSU-Michigan--bioreactordiagram.png)
![](https://static.igem.org/mediawiki/2017/0/05/MSU-Michigan_bioreactorassembly.png)
We modelled the small single cell bioreactors on drawing programs before building many of them. We made sure that they were cost effective and are reasonable to build. A per unit cost is presented in full detail in the drop down list below where the final total cost is $7.43. This does not include a way to measure the current, which would be about another $30.00 depending on which Arduino board (1,2,3) you purchase and how you set it up. This would bring the total cost up to $37.43, which is well within reason for a system that can be reused and will detect harmful contaminants in water. The cost of a drinking water test can range from $50-270. While these tests can detect more contaminants than our system currently can it would only take more genetically engineered Shewanella in order to match the range of tests at a significantly more affordable price.
Single-Chamber Bioreactors
Materials:
- $0.01-$0.04/jar (Bulk discount; MIN = 10,000 units)
- Per unit: $0.04
- Cost: $2.65/8 tubes
- Per unit: $0.33
- Cost: $43.70
- Makes 500 housings
- Per unit: $0.09
- $219.34/2.5 kg
- 1.3 g/housing
- Per unit: $0.01
- 15cm/wire
- Per unit: $0.81
- $52.18 per sheet
- 30 x 30 cm (6.35mm thick)
- 25 x 25 mm/unit
- 144 electrodes per sheet
- Per unit: $0.36
- $0.05 ea.
- x 2 per unit
- Per unit: $0.10
- Per unit: $0.04
- trade size #12 (2 1/2", tapered to 2 1/8")
- Per unit: $2.31
- trade size C (3/8" tapered to 7/32")
- Per unit: $0.71
- Per stir bar: $3.96
- Bulk unit via MSU chemistry store
- Per unit: $1.60
- $48.20
- Makes 75 eletrodes
- Per unit: $0.64
- Per 500ml: $0.01
- Cost: $39.26
- Package has 100 needles
- Per unit: $0.39
-
200 mL mason jar
Find Part
-
Glass reference electrode housing
Find Part
-
Magnesia sticks (needed to complete housing)
Find Part
-
Potassium Chloride
Find Part
-
Titanium wire
Find Part
-
Carbon felt
Find Part
-
Sterile 18G needles
Find Part
-
3mL syringe
Find Part
-
Rubber stopper
Find Part
-
Rubber stopper
Find Part
-
Octogonal magnetic stir bars with pivot ring
Find Part
-
Leit-C for microscopy (carbon cement for electrodes)
Find Part
-
Xylene
Find Part
-
18G Hypodermic sampling needle (4")
Find Part
References
(1) Aristizabal, D. H.; Giraldo, D. A.; Sanchez, S.; Taborda, G.; Baeza, A. J. Phys. Conf. Ser. 2017, 365, 11001.
(2) Jannelli, N.; Anna Nastro, R.; Cigolotti, V.; Minutillo, M.; Falcucci, G. Appl. Energy 2017, 192, 543–550.
(3) Yang, Y.; Ren, H.; Ben-Tzvi, P.; Yang, X.; He, Z. Int. J. Hydrogen Energy 2017, 42 (31), 20260–20268.
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