Difference between revisions of "Team:IISER-Pune-India/Basic Part"

 
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<li><b>G13 Promoter</b>; <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2258002">BBa_K228002</a> G13 is a constitutively expressed mycobacterial promoter obtained from Mycobacterium marinum.<br />Ref: The complex architecture of mycobacterial promoters Mae Newton-Foot, Nicolaas C. Gey van Pittius</li>
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<li><b>pL-pLeft promoter</b>; <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2258000">BBa_K228000</a> Hybrid promoter designed by Yash Jawale, which acts as a shuttle promoter between E.coli and Mycobacterium. pL initiates transcription in E.coli, whereas pLeft initiates transcription in Mycobacterium.<br />Ref: 1.PL Expression System : A Prokaryotic Expression System, Invitrogen manual 2.Transcriptional regulation of repressor synthesis in mycobacteriophage L5, Chadd E. Nesbit, Margaret E. Levin, Mary K. Donnelly-Wu and Graham F. HatfuU</li>
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<li><b>pLeft Promoter</b>; <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2258001">BBa_K228001</a> Constitutive promoter for mycobacterium isolated from mycobacteriophage L5.<br />Ref: Transcriptional regulation of repressor synthesis in mycobacteriophage L5, Chadd E. Nesbit, Margaret E. Levin, Mary K. Donnelly-Wu and Graham F. HatfuU</li>
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Latest revision as of 00:54, 2 November 2017

Results
  • G13 Promoter; BBa_K228002 G13 is a constitutively expressed mycobacterial promoter obtained from Mycobacterium marinum.
    Ref: The complex architecture of mycobacterial promoters Mae Newton-Foot, Nicolaas C. Gey van Pittius
  • ::
  • pL-pLeft promoter; BBa_K228000 Hybrid promoter designed by Yash Jawale, which acts as a shuttle promoter between E.coli and Mycobacterium. pL initiates transcription in E.coli, whereas pLeft initiates transcription in Mycobacterium.
    Ref: 1.PL Expression System : A Prokaryotic Expression System, Invitrogen manual 2.Transcriptional regulation of repressor synthesis in mycobacteriophage L5, Chadd E. Nesbit, Margaret E. Levin, Mary K. Donnelly-Wu and Graham F. HatfuU
  • ::
  • pLeft Promoter; BBa_K228001 Constitutive promoter for mycobacterium isolated from mycobacteriophage L5.
    Ref: Transcriptional regulation of repressor synthesis in mycobacteriophage L5, Chadd E. Nesbit, Margaret E. Levin, Mary K. Donnelly-Wu and Graham F. HatfuU