Difference between revisions of "Team:UNOTT"

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==University of Nottingham==
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{{:Team:UNOTT/Template/NavBarTest}}
  
  
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<h2>Keycoli</h2>
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<html >
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'''The first biological password that changes over time!
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<head>
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*Microorganism is transformed using BioBricks to produce secondary metabolites, initially the metabolites will be a simpler product such as fluorescent proteins. The secondary metabolites will be produced in a unique and random configuration and as as our "key". In order to produce this randomness, shuffling of metabolite expression levels via transposons or error prone RNase will be applied. To produce a unique configuration of the metabolite varying promoter expression levels will produce unique metabolites. This key will be used to open locked mechanism such as safes and secure doors.
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  <meta charset="UTF-8">
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*For the key to be practical it would need to be portable, this is where our key transport device comes in. It will consist of a similar design to a chemostat. Our Key colony metabolites will degrade a desired amount of time before they must be renewed from the Lock colony, when this occurs the configuration of the key is shuffled once again to ensure the key and lock are changing.
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  <meta name="viewport" content="width=device-width, initial-scale=1">
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*Once the key has been transported to the locked object a juxtaposition of a detection technique such as gas chromotography or mass spectrometry and data comparison software will compare the secondary metabolites of the "key" microorganism to the "reference/lock" colony. If the metabolies of both colonies exceeds a threshold of similarity the locked object will become unlocked.
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  <link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/normalize/5.0.0/normalize.min.css">
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<h2>Project Overview</h2>
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<style>
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'''
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      @import url(https://fonts.googleapis.com/css?family=Lato:300,400,300italic,400italic);
  
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<h3>Radiation altered plasmid</h3>
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      /* Base Styles
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      ===================================*/
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      body {
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      color: #183532;
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      font-family: 'Lato', sans-serif;
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      font-weight: 400;
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      line-height: 1.8;
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        background-color: #fff;
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        width: auto;
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        position:absolute;
  
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[[Image:Nottsplasmidr.png|600px|]]
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      }
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[[Image:Nottsplasmidt.png|600px|]]
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<h2>Plan</h2>
 
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Plan here
 
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<h2>Bacterial Chassis type</h2>
 
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[[Image:Nottsadvdis.png]]
 
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<h2>Transposon selection</h2>
 
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Tn7 Transposon used due to specific target site selection for its transposition, which is impossible in other transposon species, without this modular increases in promoter activity could not be achieved as random insertions would create a gradient rather than step wise expression pattern of proteins.
 
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[[Image:Nottstranspose.png|300px|thumb|right|]]
 
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<h2>Promoter selection</h2>
 
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Promoters are selected to have a greatly varied production of products. These will then allow easy recognition of each level 0-3
 
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<h2>Bacterial Key Transport</h2>
 
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There is a need for a transport mechanism for the key. This presents problems depennding on the bacteria used.
 
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*In Ecoli Our key transport system would need to:
 
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Keep our colonies alive for a few days
 
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Potentially could freeze. Freezing is one of the best ways to store bacteria.
 
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The lower the temperature the longer the culture will retain viable cells
 
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PROBLEM: Ice can damage cells due to localised accumulation of salt, it can also rupture membranes.
 
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SOLUTION: Use glycerol as a cryoprotectant
 
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Inhibit conjugation/recombination/genome shuffling
 
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Synthetic fatty acids prevents plasmid-mediated horizontal gene transfer (M.Getino, Et al,. 2015)
 
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Act against the induction of mutations
 
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*In Clostridium, spore storage could be used, however this presents the problem of spore awakening and time taken for product synthesis
 
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<h2>Key comparison Environment</h2>
 
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A method by which the comparative environment for mother and daughter key colonies can be compared in alternative environments
 
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<h2>Possible Metabolites</h2>
 
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<h4>LIMS1</h4>
 
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*Limonene synthase 1 from Citrus limon (accession AF514287). Synthesised by Geneart. Limonene synthase converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent. Note: several codons were altered silently to remove several forbidden restriction sites. The N-terminal region (plastid targeting sequence) was removed, removing the first 51 amino acids and replacing codon 52 with the start codon ATG.
 
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For a diagram of limonene synthesis, and the synthesis of other lemon flavour compounds see Edinburgh Yoghurt Wiki
 
  
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<h4>SAM:benzoic acid carboxyl methyltransferase</h4> converts benzoic acid to methyl benzoate (floral odor)
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      /* Layout Styles
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BBa_J45002 encodes SAM benzoic acid carboxyl methyltransferase I derived from BAMT from Antirrhinus majus (snapdragon). BAMT catalyzes the conversion of benzoic acid to methyl benzoate. Methyl benzoate has a floral smell.
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      ===================================*/
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      section:not(:first-of-type),
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      section:not(:nth-of-type(n2)) {
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      margin-top: 80px;
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      }
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      .section-two,
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      .section-three,
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      footer {
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      margin-top: 80px;
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      }
  
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*Enzyme is active as a dimer.
 
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There are two methionine codons at the begnning of the BAMT cDNA. The BAMT resulting from amplification from the second methionine shows a 2.4 times higher specific activity than from the first methionine in protein purified from cell lysate; on the other hand, BAMT purified from E. coli shows no difference in specific activity (from both Met codons). The Km and kcat are the same from both codons in E. coli.
 
  
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*Inhibition by SAH was competitive with respect to SAM and noncompetitive with respect to benzoic acid. SAM appears to be the first substrate to bind to the enzyme. Methyl benzoate is released first and SAH last.
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BAMT activity may be regulated by the intracellular SAM/SAH concentration ratio rather than benzoic acid availability.
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<h4>alcohol acetyltransferase I</h4>
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      /* Cover / Jumbotron Styles
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      ===================================*/
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      section .cover {
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      padding-top: 70px;
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      background-repeat: no-repeat;
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      background-size: cover;
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      height: 100vh;
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        width: 100vw;
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      display: -ms-flexbox;
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      display: flex;
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 +
      }
  
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*BBa_J45014 encodes alcohol acetyltransferase I derived from ATF1 from Saccharomyces cerevisiae. ATF1 catalyzes the conversion of isoamyl alcohol to isoamyl acetate. Isoamyl acetate has a banana smell. converts isoamyl alcohol to isoamyl acetate (banana odor)
 
  
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*[Note that this is not aspartate amino transferase (also called "AATase"), and that "ATF1" also refers to "Activating Transcription Factor 1" in humans (an entirely different protein that is homologous to "Atf1" in mouse).]
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      /* Section One Styles
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Sequence and Features
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      ===================================*/
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      .section-one-content {
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      padding: 1em 1em;
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      color: #ffffff;
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      font-weight: 300;
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        height:350px;
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      }
 +
      .section-one-content h1 {
 +
      margin: 0 0 0px;
 +
      }
 +
      #section-one-left {
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      background: #183532;
 +
      }
 +
      #section-one-middle {
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      background: #244F4A;
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      }
 +
      #section-one-right {
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      background: #2A5C56;
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 +
      }
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 +
      .section-two,
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      .section-three {
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      background: #fff;
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      }
 +
 
 +
      /* CSS Image Styles
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      ====================================================*/
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      .css-img-wrapper {
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          display: -webkit-box;
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          display: -ms-flexbox;
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          display: flex;
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          -webkit-box-orient: vertical;
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          -webkit-box-direction: normal;
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              -ms-flex-direction: column;
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                  flex-direction: column;
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          -ms-flex-wrap: nowrap;
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              flex-wrap: nowrap;
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          -webkit-box-pack: center;
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              -ms-flex-pack: center;
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                  justify-content: center;
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          -ms-flex-line-pack: center;
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              align-content: center;
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          -webkit-box-align: center;
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              -ms-flex-align: center;
 +
                  align-items: center;
 +
      }
 +
      .img-designers,
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      .img-developers {
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      height: 400px;
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      width: 400px;
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      background-position: center center;
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      background-size: cover;
 +
      }
 +
      .img-designers {
 +
      background: #183532;
 +
      }
 +
      .img-developers {
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      background: #2A5C56;
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      }
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 +
 
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 +
      /* Media Styles
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      ===================================*/
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      @media (min-width: 480px) {
 +
      body {
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      font-size: 16px;
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 +
               
 +
      }
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      }
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      @media (min-width: 768px) {
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      .section-one-content {
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      padding: 40px 40px;
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      }
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      }
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      @media (min-width: 992px) {
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      .section-one-content {
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      padding: 40px 20px;
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      }
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      }
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      @media (min-width: 1200px) {
 +
      .section-one-content {
 +
      padding: 40px 40px;
 +
      }
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      }
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@media only screen and (max-width: 500px) {
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    .section.cover {
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        max-width:100%;
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    }
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    .cover {
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        max-width:100%;
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    }
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}
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h1 {
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          font-size: 300px;
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          font-color: #ffffff;
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    }
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.maintext {
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position: absolute;
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left: 33%%;
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top: 10%;
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}
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.maintext2 {
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position: absolute;
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left: 33%;
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top: 40%;
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}
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.mainimage{
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max-width: 100%;
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height: auto;
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}
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</style>
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</head>
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<body>
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  <body data-spy="scroll" data-target="#myScrollspy" class="">
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<!-- Full Screen Landing Section -->
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<div class="cover">
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<img class="mainimage"src="https://static.igem.org/mediawiki/2017/1/12/T--UNOTT--CoverImg.png">
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</div>
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<!-- //Close Full Screen Landing Section -->
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<!-- Section One; as seen on many site 3 main option sections for content -->
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<section class="container-fluid section-one">
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<div class="row">
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<div class="col-md-4 section-one-content" id="section-one-left">
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<h1>What?</h1>
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<p> An unpredicatable security system consisting of bacteria. <br>
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</p>
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</div>
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<div class="col-md-4 section-one-content" id="section-one-middle">
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<h1>Why?</h1>
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<p> To secure customer accounts that require high-end security.<br>
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</p>
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</div>
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<div class="col-md-4 section-one-content" id="section-one-right">
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<h1>How?</h1>
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<p>Unique expression of fluorescent proteins linked to CRISPR dCas9 interference.</p>
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</div>
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</div>
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</section>
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<!-- //Close Section One -->
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<!--// Section Three -->
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<section class="container section-three" id="spydevelopers">
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<div class="row">
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<img src="https://static.igem.org/mediawiki/2017/0/08/T--UNOTT--Slogan.png" style="width:100%;height:auto;">
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</div>
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</section>
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<!--//Close Section Three -->
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<br> </br>
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</body>
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</html>
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{{:Team:UNOTT/Template/Footer}}

Latest revision as of 00:57, 2 November 2017


What?

An unpredicatable security system consisting of bacteria.

Why?

To secure customer accounts that require high-end security.

How?

Unique expression of fluorescent proteins linked to CRISPR dCas9 interference.