Difference between revisions of "Team:Fudan/Basic Part"

(Prototype team page)
 
 
(27 intermediate revisions by 4 users not shown)
Line 1: Line 1:
−
{{Fudan}}
+
{{Fudan/Base}}
 +
{{Fudan/PageBase}}
 +
{{Fudan/PageHeader}}
 +
 
 
<html>
 
<html>
  
−
<div class="column full_size judges-will-not-evaluate">
+
<style media="screen">
−
<h3>★  ALERT! </h3>
+
  .gray-block:{
−
<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
+
    padding:0px;
−
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
+
  }
−
</div>
+
</style>
−
<div class="clear"></div>
+
<script>
 +
$(document).ready(function(){
 +
  $('#HQ_page').append(new BorderAnimation(
 +
    ['https://static.igem.org/mediawiki/2017/a/a2/T--Fudan--synNotch-frame.png',
 +
    'https://static.igem.org/mediawiki/2017/a/af/T--Fudan--Stripe-frame.png',
 +
    'https://static.igem.org/mediawiki/2017/f/ff/T--Fudan--synTF-frame.png',
 +
    'https://static.igem.org/mediawiki/2017/4/46/T--Fudan--HCC2-frame.png'],
 +
  ['align-1','align-2','align-3','align-4'],3,0,-18).getElement());
 +
});
 +
</script>
  
−
<div class="column full_width">
+
<div class="block gray-block">
 +
<table class="light-green">
 +
  <colgroup span="1" width="20%"></colgroup>
 +
  <colgroup span="1" width="20%"></colgroup>
 +
  <colgroup span="1" width="20%"></colgroup>
 +
  <colgroup span="1" width="20%"></colgroup>
 +
  <colgroup span="1" width="20%"></colgroup>
 +
<tr>
 +
    <td>
 +
      <center>
 +
        <a href="https://2017.igem.org/Team:Fudan/Demonstrate"><img class="align" id="align-1" src='https://static.igem.org/mediawiki/2017/9/93/T--Fudan--synNotch.png' /></a>
 +
      </center>
 +
    </td>
 +
    <td>
 +
      <center>
 +
        <a href="https://2017.igem.org/Team:Fudan/Basic_Part"><img class="align" id="align-4" src='https://static.igem.org/mediawiki/2017/2/24/T--Fudan--hccOrange.png' /></a>
 +
      </center>
 +
    </td>
 +
    <td>
 +
      <center>
 +
        <a href="https://2017.igem.org/Team:Fudan/Composite_Part"><img class="align" id="align-3" src='https://static.igem.org/mediawiki/2017/6/66/T--Fudan--synTF.png' /></a>
 +
      </center>
 +
    </td>
  
 +
    <td>
 +
      <center>
 +
        <a href="https://2017.igem.org/Team:Fudan/Part_Collection"><img class="align" id="align-2" src='https://static.igem.org/mediawiki/2017/0/0c/T--Fudan--stripe.png' /></a>
 +
      </center>
 +
    </td>
 +
</tr>
  
−
<h1>Basic Parts</h1>
+
</table>
 +
</div>
  
−
<p>
+
<div class="block main-text sub-title  dark-blue">
−
A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
+
  <h class="sup-title dark-blue"></br>Basic Part – surEGFP</br></h>
 +
</div>
 +
 
 +
<div class="block main-text main-text">
 +
  <h class="highlight-title dark-blue"></br></br>For this year’s iGEM competition, our favorite basic part is surEGFP </br>(BBa_K2446051) </h>
 +
  <p></br>This part is a surface-expressed EGFP which can be used to create heterogeneous antigen density expressing cell model. Our team is very excited to be the first to bring the idea of antigen density heterogeneity to the iGEM competition, and propose a tri-response immunotherapy, <a href="https://2017.igem.org/Team:Fudan/Applied_Design">SwordS</a>.
 
</p>
 
</p>
 +
</div>
  
−
<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
 
−
<br>
 
−
<h3>Best Basic Part Special Prize</h3>
 
  
−
<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are *many* opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
 
  
−
<br><br>
+
<div class="block main-text main-text">
−
<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
+
  <h class="highlight-title dark-blue" id="Antigen"></br></br>How surEGFP works</h>
−
<br>
+
  <p>Surface-expressed EGFP (surEGFP) is built by fusing CD8α signal peptid and EGFP to the transmembrane region of CD86 (NM_175862) from N terminal to C terminal.
 +
CD8α peptide guides EGFP to be translocated via <a target="_blank" href="https://en.wikipedia.org/wiki/Sec61">Sec61 into the <a href="https://en.wikipedia.org/wiki/Endoplasmic_reticulum" target="_blank">endoplasmic reticulum (ER)</a> lumen, while transmembrane region of CD86 embeds EGFP on the plasma membrane. Below, two confocal optical slices with 0.5 micron apart are shown, and the yellow arrowheads point to ER lumen/membrane with surEGFP. EGFP bleach really fast in the cell culture media and we could obtain a strong signal after surEGFP export out of the cell and expose to the external environment. We confirmed the cell surface exposed surEGFP by immunostaining with antibody against GFP, without permeabilizing the cells.
 +
</p>
 +
</div>
  
 +
<div align="center">
 +
<img width="60%" src="https://static.igem.org/mediawiki/2017/7/74/T--Fudan--BPFigure.png">
 +
</div>
  
  
−
<div class="highlight">
+
<div class="block main-text main-text">
−
<h4>Note</h4>
+
  <h class="highlight-title dark-blue" id="Antigen"></br></br>See More</h>
−
<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
+
<p>
 +
Integrated information about surEGFP posted on <a href="https://2017.igem.org/Team:Fudan/Demonstrate">Demonstrate</a> page: Antigen density heterogeneity cell model. Or you can alternatively check the <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446051">(BBa_K2446051)</a> on the Registry of Standard Biological Parts website.
 +
</p>
 +
<p>
 +
We listed other basic part we constructed this year below.  
 +
</p>
 +
</div>
  
  
 +
<style type="text/css">
 +
.tg  {border-collapse:collapse;border-spacing:0;}
 +
.tg td{font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;}
 +
.tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;}
 +
.tg .tg-yw4l{vertical-align:top}
 +
</style>
 +
 +
<table class="tg" width="40%" style="border-collapse:separate; border-spacing:20px 10px;>
 +
  <colgroup span="1" width="50%"></colgroup>
 +
  <colgroup span="1" width="50%"></colgroup>
 +
  <tr>
 +
    <td>2_ZF42-10/43-8 binding site</td>
 +
    <td ><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446001>(BBa_K2446001)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>2_ZF54-8/43-8 binding site</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446002>(BBa_K2446002)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>2_ZFHD1 binding site</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446003>(BBa_K2446003)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>2_PIP binding site</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446004>(BBa_K2446004)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>4_ZF_42-10 binding site</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446005>(BBa_K2446005)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>4_ZF_43-8 binding site</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446006>(BBa_K2446006)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>4_ZF_54-8 binding site</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446007>(BBa_K2446007)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>4_ZF_21-16 binding site</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446008>(BBa_K2446008)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>4_ZF54-8/43-8 binding site</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446009>(BBa_K2446009)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>4_ZFHD1 binding site</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446010>(BBa_K2446010)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>4_PIP binding site</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446011>(BBa_K2446011)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>8_ZF_42-10 binding site</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446012>(BBa_K2446012)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>8_ZF_43-8 binding site</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446013>(BBa_K2446013)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>8_ZF_54-8 binding site</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446014>(BBa_K2446014)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>8_ZF_21-16 binding site</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446015>(BBa_K2446015)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>8_ZF_54-8/43-8 binding site</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446016>(BBa_K2446016)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>8_PIP binding site</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446017>(BBa_K2446017)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>ZF_GAl4_KRAB</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446037>(BBa_K2446037)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>ZF_PIP_KRAB</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446038>(BBa_K2446038)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>ZF_21-16KRAB</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446039>(BBa_K2446039)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>ZF_42-10_KRAB</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446040>(BBa_K2446040)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>ZF_43-8_KRAB</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446041>(BBa_K2446041)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>ZF_54-8_KRAB</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446042>(BBa_K2446042)</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>ZFHD1_KRAB</td>
 +
    <td><a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2446043>(BBa_K2446043)</a></td>
 +
  </tr>
 +
</table>
 +
 +
<div>
 +
<p></br></br></p>
 
</div>
 
</div>
  
  
 
</html>
 
</html>
 +
 +
{{Fudan/PageFooter}}

Latest revision as of 01:22, 2 November 2017

Basic Part – surEGFP


For this year’s iGEM competition, our favorite basic part is surEGFP
(BBa_K2446051)


This part is a surface-expressed EGFP which can be used to create heterogeneous antigen density expressing cell model. Our team is very excited to be the first to bring the idea of antigen density heterogeneity to the iGEM competition, and propose a tri-response immunotherapy, SwordS.



How surEGFP works

Surface-expressed EGFP (surEGFP) is built by fusing CD8α signal peptid and EGFP to the transmembrane region of CD86 (NM_175862) from N terminal to C terminal. CD8α peptide guides EGFP to be translocated via Sec61 into the endoplasmic reticulum (ER) lumen, while transmembrane region of CD86 embeds EGFP on the plasma membrane. Below, two confocal optical slices with 0.5 micron apart are shown, and the yellow arrowheads point to ER lumen/membrane with surEGFP. EGFP bleach really fast in the cell culture media and we could obtain a strong signal after surEGFP export out of the cell and expose to the external environment. We confirmed the cell surface exposed surEGFP by immunostaining with antibody against GFP, without permeabilizing the cells.



See More

Integrated information about surEGFP posted on Demonstrate page: Antigen density heterogeneity cell model. Or you can alternatively check the (BBa_K2446051) on the Registry of Standard Biological Parts website.

We listed other basic part we constructed this year below.

2_ZF42-10/43-8 binding site (BBa_K2446001)
2_ZF54-8/43-8 binding site (BBa_K2446002)
2_ZFHD1 binding site (BBa_K2446003)
2_PIP binding site (BBa_K2446004)
4_ZF_42-10 binding site (BBa_K2446005)
4_ZF_43-8 binding site (BBa_K2446006)
4_ZF_54-8 binding site (BBa_K2446007)
4_ZF_21-16 binding site (BBa_K2446008)
4_ZF54-8/43-8 binding site (BBa_K2446009)
4_ZFHD1 binding site (BBa_K2446010)
4_PIP binding site (BBa_K2446011)
8_ZF_42-10 binding site (BBa_K2446012)
8_ZF_43-8 binding site (BBa_K2446013)
8_ZF_54-8 binding site (BBa_K2446014)
8_ZF_21-16 binding site (BBa_K2446015)
8_ZF_54-8/43-8 binding site (BBa_K2446016)
8_PIP binding site (BBa_K2446017)
ZF_GAl4_KRAB (BBa_K2446037)
ZF_PIP_KRAB (BBa_K2446038)
ZF_21-16KRAB (BBa_K2446039)
ZF_42-10_KRAB (BBa_K2446040)
ZF_43-8_KRAB (BBa_K2446041)
ZF_54-8_KRAB (BBa_K2446042)
ZFHD1_KRAB (BBa_K2446043)