Difference between revisions of "Team:MSU-Michigan/Applied Design"

 
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<h1><font color="Green"; size="11"> <b>Applied Design</b></font></h1>
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<h2><font color="grey"; size="8"><b>Single-Chamber Biosensors</b></font></h2>
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<p>MSU-iGEM 2017 developed a cost effective, applied biosensor. We designed this biosensor to detect water contaminants in a variety of circumstances and be used by multiple audiences. The biosensor is user friendly even to common consumers and high school students as well. The simple design promotes easy assembly and can be used as an education tool for high schools to show the possibilities of synthetic biology. In detecting water contaminants, the biosensor is durable and portable for scientists to use in field testing and consumers to use at home. The system is designed for initial testing for contaminants that are not regulated or tested for by the EPA. The biosensor provides a cost effective, rapid initial to test if the water must be analyzed by more costly measures. The design also utilizes        <a href="https://www.arduino.cc/">Arduino boards</a> (1,2,3) to apply the needed potential so the bacteria can produce electricity.</p>
  
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<h1>Future Applications</h1>
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<img src="https://static.igem.org/mediawiki/2017/thumb/0/02/MSU-Michiganbluelight.jpeg/800px-MSU-Michiganbluelight.jpeg.png" style="width:100%">
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<h2><font color="grey"; face="Tw Cen MT"; size="5"><b>Microbial Fuel Cells</b></font></h2>
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    <b>Paper MFC Procedure</b>
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        <ul> <font face="Tw Cen MT"; size="4">
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<p><b>Purpose:</b> Create ultra-low cost MFCs to be innoculated with Shewanella Oneidensis MR-1 and then induced with a selected compound</p>
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Materials:
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<li>Whatman Paper</li>
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<li>Scissors</li>
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<li>Razor</li>
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<li>Aluminum Foil</li>
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<li>8B Graphite Pencils</li>
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<li>Carbon Cement</li>
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<li>Crayon</li>
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<li>Superglue (cyanoacrylate)</li>
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<li>Parchment Paper</li>
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Procedure:
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<li>Cut out  30mm x 30mm squares of whatman paper (6 per reactor) and one piece of parchment paper of the same size.</li>
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<li>Color the sides of five pieces of whatman paper (5mm in from edges) with crayon (both sides). One piece can be set aside, this will be the blank.</li>
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<li>Fully Color one piece of whatman paper.  This will be the cover. </li>
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<li>Draw on the center of two pieces of paper with 8B graphite pencil for at least five repititions.  These will be the anode and cathode.</li>
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<li>Cut out 2cm long 1cm wide strips of aluminum foil.
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<li>Lightly superglue aluminum foil to wax part of both anode and cathode.  Allow foil to extend 1cm onto anode/cathode.
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<li>Paint cement glue onto the anode and cathode.  Ensure proper ventilation and safety.  Allow to dry a minimum of five hours.
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<li>Cut the center out of two pieces of colored whatman paper. These will be the chamber pieces.</li>
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<li>Superglue the whatman paper together with small dabs of glue on only the corners of the paper in the order of cover, chamber, chamber, cathode (with foil facing up), blank, parchment paper, anode(with foil facing down).</li></ul>
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<h4>Prototype Testing</h4>
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<img alt="File:MSU-Michigan mtrBcurrent.png" src="/wiki/images/thumb/5/50/MSU-Michigan_mtrBcurrent.png/600px-MSU-Michigan_mtrBcurrent.png" style="width:100%;max-width:500px"  srcset="/wiki/images/thumb/5/50/MSU-Michigan_mtrBcurrent.png/900px-MSU-Michigan_mtrBcurrent.png 1.5x, /wiki/images/thumb/5/50/MSU-Michigan_mtrBcurrent.png/1200px-MSU-Michigan_mtrBcurrent.png 2x">
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<h1>Applied Design</h1>
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<h3>Best Applied Design Special Prize</h3>
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<p>This is a prize for the team that has developed a synbio product to solve a real world problem in the most elegant way. The students will have considered how well the product addresses the problem versus other potential solutions, how the product integrates or disrupts other products and processes, and how its lifecycle can more broadly impact our lives and environments in positive and negative ways.
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To compete for the <a href="https://2017.igem.org/Judging/Awards">Best Applied Design prize</a>, please describe your work on this page and also fill out the description on the <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a>.
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You must also delete the message box on the top of this page to be eligible for this prize.
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<p>This is what happens when a hydrogen gas bubble forms and pushes the media up onto the electrode as well as having the potassium solution spill from the bioreactor due to a poor connection of the reference electrode.</p>
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<h1>Upgrading Design</h1>
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After seeing the initial current production, we modified the design by adding a larger syringe housing for the cathode up to the present 3 ml syringe. This protected against hydrogen gas pockets building up and shorting the circuit by pushing the media down off the titanium wire. We also standardized adding 18 gauge needles to this housing area to promote better ventilation of the hydrogen gas. This eliminated the main problem that was distorting the data. We also made new rubber stoppers that provided a better fit into the glass reference housing for more stable background data. This allowed us to produce smooth current graphs throughout our entire project.
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<a href="https://2017.igem.org/Team:MSU-Michigan/Results" class="w3-button w3-xlarge w3-padding-large w3-green w3-ripple w3-round w3-hover-white">Our Results</a>
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<h5>Inspiration</h5>
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<a href="https://2017.igem.org/Team:MSU-Michigan/Demonstrate" class="w3-button w3-green w3-xlarge w3-padding-large w3-ripple w3-round w3-hover-white">Demonstrating the Design</a>
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<p>Take a look at what some teams accomplished for this prize.</p>
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<ul>
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<li><a href="https://2016.igem.org/Team:NCTU_Formosa/Design">2016 NCTU Formosa</a></li>
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<li><a href="https://2016.igem.org/Team:HSiTAIWAN/Product?locationId=Design">2016 HSiTAIWAN</a></li>
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<li><a href="https://2016.igem.org/Team:Pasteur_Paris/Design">2016 Pasteur Paris</a></li>
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<h1>References</h1>
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<br>(1)  Aristizabal, D. H.; Giraldo, D. A.; Sanchez, S.; Taborda, G.; Baeza, A. J. Phys. Conf. Ser. 2017, 365, 11001.
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<br>(2)  Jannelli, N.; Anna Nastro, R.; Cigolotti, V.; Minutillo, M.; Falcucci, G. Appl. Energy 2017, 192, 543–550.
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<br>(3)  Yang, Y.; Ren, H.; Ben-Tzvi, P.; Yang, X.; He, Z. Int. J. Hydrogen Energy 2017, 42 (31), 20260–20268.
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Latest revision as of 01:26, 2 November 2017

Home
Description Design Experiments InterLab Contribution Results Demonstrate Attributions
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Team Collaborations
Parts
Applied Design Hardware Measurement Model

Applied Design

Single-Chamber Biosensors

MSU-iGEM 2017 developed a cost effective, applied biosensor. We designed this biosensor to detect water contaminants in a variety of circumstances and be used by multiple audiences. The biosensor is user friendly even to common consumers and high school students as well. The simple design promotes easy assembly and can be used as an education tool for high schools to show the possibilities of synthetic biology. In detecting water contaminants, the biosensor is durable and portable for scientists to use in field testing and consumers to use at home. The system is designed for initial testing for contaminants that are not regulated or tested for by the EPA. The biosensor provides a cost effective, rapid initial to test if the water must be analyzed by more costly measures. The design also utilizes Arduino boards (1,2,3) to apply the needed potential so the bacteria can produce electricity.

Prototype Testing

File:MSU-Michigan mtrBcurrent.png

This is what happens when a hydrogen gas bubble forms and pushes the media up onto the electrode as well as having the potassium solution spill from the bioreactor due to a poor connection of the reference electrode.


Upgrading Design


After seeing the initial current production, we modified the design by adding a larger syringe housing for the cathode up to the present 3 ml syringe. This protected against hydrogen gas pockets building up and shorting the circuit by pushing the media down off the titanium wire. We also standardized adding 18 gauge needles to this housing area to promote better ventilation of the hydrogen gas. This eliminated the main problem that was distorting the data. We also made new rubber stoppers that provided a better fit into the glass reference housing for more stable background data. This allowed us to produce smooth current graphs throughout our entire project.

Our Results Demonstrating the Design



References


(1) Aristizabal, D. H.; Giraldo, D. A.; Sanchez, S.; Taborda, G.; Baeza, A. J. Phys. Conf. Ser. 2017, 365, 11001.
(2) Jannelli, N.; Anna Nastro, R.; Cigolotti, V.; Minutillo, M.; Falcucci, G. Appl. Energy 2017, 192, 543–550.
(3) Yang, Y.; Ren, H.; Ben-Tzvi, P.; Yang, X.; He, Z. Int. J. Hydrogen Energy 2017, 42 (31), 20260–20268.


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