Difference between revisions of "Team:MSU-Michigan/Applied Design"

 
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<font color="Green"; face="Verdana"; size="11"> <b>Applied Design</b></font>
 
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<h2><font color="grey"; face="Tw Cen MT"; size="8"><b>Single-Chamber Biosensors</b></font></h2>
 
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<p>MSU-iGEM 2017 developed a cost effective, applied biosensor. We designed this biosensor to detect water contaminants in a variety of circumstances and be used by multiple audiences. The biosensor is user friendly even to common consumers and high school students as well. The simple design promotes easy assembly and can be used as an education tool for high schools to show the possibilities of synthetic biology. In detecting water contaminants, the biosensor is durable and portable for scientists to use in field testing and consumers to use at home. The system is designed for initial testing for contaminants that are not regulated or tested for by the EPA. The biosensor provides a cost effective, rapid initial to test if the water must be analyzed by more costly measures. The design also utilizes Arduino boards to apply the needed potential so the bacteria can produce electricity.</p>
 
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<h1><font color="Green"; size="11"> <b>Applied Design</b></font></h1>
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<h2><font color="grey"; size="8"><b>Single-Chamber Biosensors</b></font></h2>
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<p>MSU-iGEM 2017 developed a cost effective, applied biosensor. We designed this biosensor to detect water contaminants in a variety of circumstances and be used by multiple audiences. The biosensor is user friendly even to common consumers and high school students as well. The simple design promotes easy assembly and can be used as an education tool for high schools to show the possibilities of synthetic biology. In detecting water contaminants, the biosensor is durable and portable for scientists to use in field testing and consumers to use at home. The system is designed for initial testing for contaminants that are not regulated or tested for by the EPA. The biosensor provides a cost effective, rapid initial to test if the water must be analyzed by more costly measures. The design also utilizes        <a href="https://www.arduino.cc/">Arduino boards</a> (1,2,3) to apply the needed potential so the bacteria can produce electricity.</p>
  
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<h4>Prototype Testing</h4>
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<img alt="File:MSU-Michigan mtrBcurrent.png" src="/wiki/images/thumb/5/50/MSU-Michigan_mtrBcurrent.png/600px-MSU-Michigan_mtrBcurrent.png" style="width:100%;max-width:500px"  srcset="/wiki/images/thumb/5/50/MSU-Michigan_mtrBcurrent.png/900px-MSU-Michigan_mtrBcurrent.png 1.5x, /wiki/images/thumb/5/50/MSU-Michigan_mtrBcurrent.png/1200px-MSU-Michigan_mtrBcurrent.png 2x">
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<p>This is what happens when a hydrogen gas bubble forms and pushes the media up onto the electrode as well as having the potassium solution spill from the bioreactor due to a poor connection of the reference electrode.</p>
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<h1>Upgrading Design</h1>
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After seeing the initial current production, we modified the design by adding a larger syringe housing for the cathode up to the present 3 ml syringe. This protected against hydrogen gas pockets building up and shorting the circuit by pushing the media down off the titanium wire. We also standardized adding 18 gauge needles to this housing area to promote better ventilation of the hydrogen gas. This eliminated the main problem that was distorting the data. We also made new rubber stoppers that provided a better fit into the glass reference housing for more stable background data. This allowed us to produce smooth current graphs throughout our entire project.
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<a href="https://2017.igem.org/Team:MSU-Michigan/Results" class="w3-button w3-xlarge w3-padding-large w3-green w3-ripple w3-round w3-hover-white">Our Results</a>
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<a href="https://2017.igem.org/Team:MSU-Michigan/Demonstrate" class="w3-button w3-green w3-xlarge w3-padding-large w3-ripple w3-round w3-hover-white">Demonstrating the Design</a>
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<h1>References</h1>
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<br>(1)  Aristizabal, D. H.; Giraldo, D. A.; Sanchez, S.; Taborda, G.; Baeza, A. J. Phys. Conf. Ser. 2017, 365, 11001.
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<br>(2)  Jannelli, N.; Anna Nastro, R.; Cigolotti, V.; Minutillo, M.; Falcucci, G. Appl. Energy 2017, 192, 543–550.
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<br>(3)  Yang, Y.; Ren, H.; Ben-Tzvi, P.; Yang, X.; He, Z. Int. J. Hydrogen Energy 2017, 42 (31), 20260–20268.
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Latest revision as of 01:26, 2 November 2017

Home
Description Design Experiments InterLab Contribution Results Demonstrate Attributions
Safety
Engagement Gold Integrated Silver
Team Collaborations
Parts
Applied Design Hardware Measurement Model

Applied Design

Single-Chamber Biosensors

MSU-iGEM 2017 developed a cost effective, applied biosensor. We designed this biosensor to detect water contaminants in a variety of circumstances and be used by multiple audiences. The biosensor is user friendly even to common consumers and high school students as well. The simple design promotes easy assembly and can be used as an education tool for high schools to show the possibilities of synthetic biology. In detecting water contaminants, the biosensor is durable and portable for scientists to use in field testing and consumers to use at home. The system is designed for initial testing for contaminants that are not regulated or tested for by the EPA. The biosensor provides a cost effective, rapid initial to test if the water must be analyzed by more costly measures. The design also utilizes Arduino boards (1,2,3) to apply the needed potential so the bacteria can produce electricity.

Prototype Testing

File:MSU-Michigan mtrBcurrent.png

This is what happens when a hydrogen gas bubble forms and pushes the media up onto the electrode as well as having the potassium solution spill from the bioreactor due to a poor connection of the reference electrode.


Upgrading Design


After seeing the initial current production, we modified the design by adding a larger syringe housing for the cathode up to the present 3 ml syringe. This protected against hydrogen gas pockets building up and shorting the circuit by pushing the media down off the titanium wire. We also standardized adding 18 gauge needles to this housing area to promote better ventilation of the hydrogen gas. This eliminated the main problem that was distorting the data. We also made new rubber stoppers that provided a better fit into the glass reference housing for more stable background data. This allowed us to produce smooth current graphs throughout our entire project.

Our Results Demonstrating the Design



References


(1) Aristizabal, D. H.; Giraldo, D. A.; Sanchez, S.; Taborda, G.; Baeza, A. J. Phys. Conf. Ser. 2017, 365, 11001.
(2) Jannelli, N.; Anna Nastro, R.; Cigolotti, V.; Minutillo, M.; Falcucci, G. Appl. Energy 2017, 192, 543–550.
(3) Yang, Y.; Ren, H.; Ben-Tzvi, P.; Yang, X.; He, Z. Int. J. Hydrogen Energy 2017, 42 (31), 20260–20268.


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