Difference between revisions of "Team:SSTi-SZGD/Safety"

 
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<title>SSTi-SZGD---Safety</title>
 
<title>SSTi-SZGD---Safety</title>
 
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Description">Description</a>
 
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Degradation">Degradation</a>
 
<a href="https://2017.igem.org/Team:SSTi-SZGD/Degradation">Degradation</a>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Applied_Design">Applied Design</a>
 
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Applied_Design">Business Plan</a>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/HP/Summary">Summary</a>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/HP/Silver">Summary</a>
 
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<a href="https://2017.igem.org/Team:SSTi-SZGD/HP/Silver">Outreach</a>
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<span>Guardian of the wheatland</span>
 
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<span>A product for the degradation of soil pesticide residues</span>
 
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</p>
 
 
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<div class="Content">
 
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<!--Safety-->
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<p class="introduction">
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<div class="Safety">
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There are two safety aspects related to our project, the general laboratory safety and the application safety related to GMOs.
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</p>
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<p class="title">Safety information</p>
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<p>There are two part of our project, the first part is lab risk of our project , the other is environment and market risk of GMO .</p>
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<!--Laboratory Safety and Management-->
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<div class="Laboratory">
 
<div class="Laboratory">
 
 
 
<p class="Title">Laboratory Safety and Management</p>
 
<p class="Title">Laboratory Safety and Management</p>
 
 
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<p class="Subtitle">Safety Training</p>
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<div class="content Training">
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<div class="left">
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<p>To guarantee the safety of our researchers and lab personnel, we emphasized protection while doing experiments related to bacteria, toxic reagents, fire and electricity. All researchers were required to maintain a tidy and clean working area to ensure that no harmful chemicals or traces of bacteria agents are left behind after the experiment.</p>
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<p>In our laboratory class, the first things is Safety Training, it is a so important Process for us, all the member were required wear lab coat and gloves before we start the experiment , and we have safety training by Laboratory center once a half years.</p>
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<p class="title">(1) Safety Training</p>
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<p>According to the Basic training of laboratory, First, we learned the safety level of laboratories and personal protective equipment. Then we learn the chemical Reagent classification and the GMO experiment regulations. For biosafety, we learned biological hazard, including levels of biohazard and instructions of dealing with biological trash.</p>
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<p>
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To guarantee the safety of our team members, students and lab personnel, we emphasized on precautions practices when doing experiments involved bacteria, toxic reagents, fire and electricity. All personnel were required to maintain a tidy and clean working area to ensure that no harmful chemicals or traces of bacteria agents are left behind after the experiment.
 +
<br />
 +
At the beginning of our project, the first session we had was Safety Training, which was provided by the Laboratory Center at our school. Contents covered in the first session included: Safety Level of Laboratories and personal rotective equipment (PPE); Chemical Reagent Classification, GMO experiment regulation. We also learned about biological hazard, including levels of biohazard and instructions on dealing with biological trash. Furthermore, basic laboratory practice codes are posted on the wall of our laboratory. All members were properly trained before started working in the lab, and every six months we had instructors from Laboratory Center to review the safety practice procedures.
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</p>
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<img src="https://static.igem.org/mediawiki/2017/e/e3/SSTi-SZGD_Safety_Training.jpeg"/>
 
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<img src="https://static.igem.org/mediawiki/2017/6/67/SSTi-SZGD_Safety_Laboratory_Training.png" alt="Figure"/>
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<p class="Subtitle">Safety of project</p>
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<div class="content project">
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<p class="title">(2) Safety of the project</p>
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<p>Our project is use light inducible to construct recombinant gene in E.coli Dh5a, we finish the Microbial experiment in biological cleanroom, it can avoid contaminate effectively, After finished the experiment, removal measures and residues treatment through baking or incineration and other means to destroy.</p>
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<div class="right">
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<p>
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<img src="https://static.igem.org/mediawiki/2017/3/3f/SSTi-SZGD_Safety_project.png"/>
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Our project works on E.coli DH5a strain. We conducted microbial experiments in biological clean-room with UV lights, which provide an isolated and clean environment to avoid cross-contamination with other organisms. Once experiment completed, GMOs and residues were autoclaved before disposal to bio-hazard waste.
 +
<br />
 +
Chemical reagents used in this project include pesticide standards, such as parathion, paraxon, arbendazim, etc. These pesticides are of high concentrations and highly toxic. Therefore preparation of the standards are conducted in the fume hood, and PPE is required at all time.
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</p>
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<div class="img">
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<img src="https://static.igem.org/mediawiki/2017/a/a9/SSTi-SZGD_Safety_Laboratory_Project.png" alt="Figure"/>
 
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<!--Daily-->
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<p class="Subtitle">Daily preventive measures in Laborator</p>
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<div class="Daily">
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<div class="content Daily">
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<div class="left">
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<p class="title">
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<p>In order to make sure safety of lab and the accuracy of the experimental results, we make 4S- Management. the lab are managed by 4S- Management very well.</p>
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(3) Daily preventive measures in Laboratory
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<p>Overall, all phases of general laboratory safety addressed the following:
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</p>
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<br />
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−
- Personal protective equipment: lab coats and enclosed leather shoes were to be worn at all times, and long hair had to be tied back. When working with corrosives, acids, bases, and alcohols, gloves and safety glasses were also required. Upon leaving the lab, a disinfectant hand-wash had to be used as well.
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<p>
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<br />
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In order to make sure the compliance of lab safety on a daily basis, and the accuracy of the experimental results, our laboratory is under the 4S-management.
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- Building safety: the location of fire escapes, fire evacuation plans, and First Aid Officers
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</p>
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<br />
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−
- Chemical safety: the location and appropriate storage methods of different classes of chemicals e.g. in the fume hood, flammables cabinet, and general chemical store cabinet
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<br />
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- Waste disposal: location of biological safety hazard waste containers, sharps disposal containers, autoclaving methods, and spills kits location/contents </p>
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<div class="right">
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<img src="https://static.igem.org/mediawiki/2017/d/d2/SSTi-SZGD_Safety_Daily.jpeg"/>
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<!--Overall, all phases of general laboratory safety are addressed the following:-->
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<div class="Overall">
 
 
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<p class="Headline">Overall, all phases of general laboratory safety are addressed the following:</p>
 
 
 +
<p>
 +
- Personal protective equipment: lab coats and enclosed leather shoes were to be worn at all times, and long hair had to be tied back. When working with corrosives, acids, bases, and alcohols, gloves and safety glasses were also required. Upon leaving the lab, a disinfectant hand-wash had to be used as well.
 +
<br />
 +
- Building safety: the location of fire escapes, fire evacuation plans, and First Aid Officers.
 +
<br />
 +
- Chemical safety: the location and appropriate storage methods of different classes of chemicals e.g. in the fume hood, flammables cabinet, and general chemical store cabinet.
 +
<br />
 +
- Waste disposal: location of biological safety hazard waste containers, sharps disposal containers, autoclaving methods, and spills kits location/contents.
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</p>
 
 
 
</div>
 
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<!--Environment Safety-->
 
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<div class="Environment content">
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<div class="Environment">
 
 
 
<p class="Title">Environment Safety</p>
 
<p class="Title">Environment Safety</p>
 
 
 
<div class="left">
 
<div class="left">
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<p>If our product were to be available in the real world at the conclusion of this project. there would be many biosafety issues associated with it. therefore , we should guarantee that it’s safe to humans and harmless to the environment. To be on the safe side , we plan to design a device, it’s could prevent Living organisms contact Environment directly by a 0.45um filter Membrane, Based on the characteristics of light - induced , there are also have a suicide system be designed, this system could kill host cell in time. so it could avoid biological leakage. </p>
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<p>Next step, we plan to conducted preliminary research into using yeast as a host cell. That could use to degrade the Pesticide residue  in surface of fruits and vegetables more carefully. </p>
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<p>
 +
If our products were to be available in the real world as a result of this project, there would be many biosafety issues we need to consider. We need to guarantee that it will be safe to humans as well as harmless to the environment. urrently we designed an automatic spraying system that sprays live bacteria cells to the objects. This means that GMOs will be exposed to the natural environment. At current stage we included a suicide system that could kill host cells as necessary to minimize the risk of GMO leakage. To completely eliminate the GMO contamination problem, in the future, we plan to change chassis that allows extracellular secretion of the enzyme. In that case, spraying takes place via a 0.45um filter membrane that helps trapping bacteria cells.
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</p>
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</div>
 
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<img src="https://static.igem.org/mediawiki/2017/b/bf/SSTi-SZGD_Safety_Environment.png"/>
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<img src="https://static.igem.org/mediawiki/2017/b/bf/SSTi-SZGD_Safety_Environment.png" alt="Figure"/>
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References待续。。。
 
 
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<img src="https://static.igem.org/mediawiki/2017/9/9d/SSTi-SZGD_logo.png"/>
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<img src="https://static.igem.org/mediawiki/2017/9/9d/SSTi-SZGD_logo.png" alt="SSTi-SZGD"/>
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<img src="https://static.igem.org/mediawiki/2017/e/e0/SSTi-SZGD_logo_SSTI.png"/>
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<img src="https://static.igem.org/mediawiki/2017/e/e0/SSTi-SZGD_logo_SSTI.png" alt="SSTI"/>
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<img src="https://static.igem.org/mediawiki/2017/8/8c/SSTi-SZGD_logo_USZ.png" alt="USZ"/>
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<img src="https://static.igem.org/mediawiki/2017/d/d2/SSTi-SZGD_logo_SSTIABD.png" alt="SSTIABD"/>
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<span class="left">A&nbsp;product&nbsp;for&nbsp;the&nbsp;degradation&nbsp;of&nbsp;soil&nbsp;pesticide&nbsp;residues</span>
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<span class="left">
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<span class="right">Copyright&nbsp;&copy;&nbsp;2017&nbsp;Lucky&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;power&nbsp;by&nbsp;&nbsp;IGEM&nbsp;Team&#58;SSTi-SZGD</span>
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A product&nbsp;for&nbsp;the&nbsp;degradation&nbsp;of&nbsp;soil&nbsp;pesticide&nbsp;residues
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</span>
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<span class="right">
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Copyright&nbsp;&copy;&nbsp;2017&nbsp;Lucky&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;power&nbsp;by&nbsp;&nbsp;iGEM&nbsp;Team&#58;SSTi-SZGD
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Latest revision as of 01:34, 2 November 2017

SSTi-SZGD---Safety

There are two safety aspects related to our project, the general laboratory safety and the application safety related to GMOs.

Laboratory Safety and Management

(1) Safety Training

To guarantee the safety of our team members, students and lab personnel, we emphasized on precautions practices when doing experiments involved bacteria, toxic reagents, fire and electricity. All personnel were required to maintain a tidy and clean working area to ensure that no harmful chemicals or traces of bacteria agents are left behind after the experiment.
At the beginning of our project, the first session we had was Safety Training, which was provided by the Laboratory Center at our school. Contents covered in the first session included: Safety Level of Laboratories and personal rotective equipment (PPE); Chemical Reagent Classification, GMO experiment regulation. We also learned about biological hazard, including levels of biohazard and instructions on dealing with biological trash. Furthermore, basic laboratory practice codes are posted on the wall of our laboratory. All members were properly trained before started working in the lab, and every six months we had instructors from Laboratory Center to review the safety practice procedures.

Figure

(2) Safety of the project

Our project works on E.coli DH5a strain. We conducted microbial experiments in biological clean-room with UV lights, which provide an isolated and clean environment to avoid cross-contamination with other organisms. Once experiment completed, GMOs and residues were autoclaved before disposal to bio-hazard waste.
Chemical reagents used in this project include pesticide standards, such as parathion, paraxon, arbendazim, etc. These pesticides are of high concentrations and highly toxic. Therefore preparation of the standards are conducted in the fume hood, and PPE is required at all time.

Figure

(3) Daily preventive measures in Laboratory

In order to make sure the compliance of lab safety on a daily basis, and the accuracy of the experimental results, our laboratory is under the 4S-management.

Overall, all phases of general laboratory safety are addressed the following:

- Personal protective equipment: lab coats and enclosed leather shoes were to be worn at all times, and long hair had to be tied back. When working with corrosives, acids, bases, and alcohols, gloves and safety glasses were also required. Upon leaving the lab, a disinfectant hand-wash had to be used as well.
- Building safety: the location of fire escapes, fire evacuation plans, and First Aid Officers.
- Chemical safety: the location and appropriate storage methods of different classes of chemicals e.g. in the fume hood, flammables cabinet, and general chemical store cabinet.
- Waste disposal: location of biological safety hazard waste containers, sharps disposal containers, autoclaving methods, and spills kits location/contents.

Environment Safety

If our products were to be available in the real world as a result of this project, there would be many biosafety issues we need to consider. We need to guarantee that it will be safe to humans as well as harmless to the environment. urrently we designed an automatic spraying system that sprays live bacteria cells to the objects. This means that GMOs will be exposed to the natural environment. At current stage we included a suicide system that could kill host cells as necessary to minimize the risk of GMO leakage. To completely eliminate the GMO contamination problem, in the future, we plan to change chassis that allows extracellular secretion of the enzyme. In that case, spraying takes place via a 0.45um filter membrane that helps trapping bacteria cells.

Figure

A product for the degradation of soil pesticide residues Copyright © 2017 Lucky      power by  iGEM Team:SSTi-SZGD