Difference between revisions of "Team:IISc-Bangalore/Hardware"

 
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                <li>The Problem</li>
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                <li>GCODe Mini</li>
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                <li>GCODe Pro</li>
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                <li>Results</li>
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<h1>The Problem</h1>
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<p>Measuring the growth of cells is a vital task in any biology lab across the world. Thousands of dollars are spent on spectrophotometers and analysers to measure the optical densities of cell cultures. More importantly, hundreds of researcher-hours are spent taking samples, diluting them and measuring the optical density, again and again, through the long nights of running growth curves.</p>
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<p>There do exist automatic devices that can measure OD, such as the Bioscreen C, but their prohibitive cost (in the tens of thousands of dollars), low volume capacity, and inability to handle high ODs make them non-viable as a substitute for manual pipetting in most experimental scenarios.
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<p style="text-align: center; font-size: 3.2em; padding: 45px">It's cheap. Two orders of magnitude less than alternatives.</p>
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Other machines simply lack the capabilities to ...</p>
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<p>There has to be a better way. That way is GCODe.</p>
 
  
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<p>GCODe (Growth Curve and Optical DEnsity) is an optical density measurement device that will not only take readings at pre-programmed intervals or continuously, but will also aerate and dilute the culture as required. It can even send you a message when the OD reaches a particular level, just in time to you to start the next stage of your experiment. Fundamentally, it automates the grunt work of growth curves, in a manner that allows you to walk away from the lab, secure in the knowledge that you will be alerted when your cells are ready for you.</p>
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<p>Our work stemmed from our own frustrations running growth curves night after night, and was also inspired by the OD meter built by the 2014 Aachen iGEM team. We worked incrementally, starting with a rudimentary box of wiring, designing version after version in response to feedback from the iGEM wetlab team, as they trialled our device, and other labs and professors from across IISc.</p>
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<p style="text-align: center; font-size: 3.2em; padding: 45px"> And it holds its own against the gold standard - a spectrophotometer.</p>
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<p>We currently have two versions of GCODe manufactured, tested and fully documented.
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They are:
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GCODe Pro
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GCODe Mini</p>
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<h1>GCODe Mini</h1>
 
  
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<h1>GCODe Pro</h1>
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<h1>Results</h1>
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<p style="text-align: center; font-size: 3.2em; padding: 45px">It's easy to build. A college freshman built it in two hours.</p>
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<a href="https://2017.igem.org/Team:IISc-Bangalore/HP/Silver#mini">
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<h2>a multifaceted approach to cluster bioengineered gas vesicles in vitro and enhance their flotation</h2>
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<p>Gas vesicles (GVs) are hollow protein nanostructures synthesized by phototrophic haloarchaea and cyanobacteria to regulate their flotation in aquatic habitats. Bioengineered GVs have been genetically modified for diverse purposes including ultrasonic molecular imaging, gauging cellular turgor pressures, and vaccine delivery — harnessing unique acoustic, mechanical, and surface properties of GVs — but none of their current applications exploits their most fundamental characteristic: buoyancy.</p>
 
  
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[[File:T--IISc-Bangalore--logo-new.png|300px|left]]
 
  
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<p>Our modelling indicates that clusters of GVs float several orders of magnitude better than individual GVs, as buoyancy scales with volume while Stokes’ drag scales with effective radius. Our project iFLOAT aims to improve the flotation of gas vesicles by clustering them using three distinct methods — charge-based flocculation, biotin-streptavidin interaction, and SpyCatcher-SpyTag heterodimerization — and simultaneously develop robust, reproducible flotation assays. Potential future applications of buoyant clusters of bioengineered gas vesicles include bioremediation of oil spills and flotation-based separation and purification of specific targets from mixtures.</p>
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<p style="text-align: center; font-size: 3.2em; padding: 45px">Want to build one yourself?
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We know you want to.</p>
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<a href="https://2017.igem.org/Team:IISc-Bangalore/Hardware/Documentation">
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Construction
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<p style="text-align: center; font-size: 3.2em; padding: 45px">But designing it wasn't so easy. See how we failed -
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and tried again.</p>
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Evolution
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Click here to continue to the GCODe Mini Page
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Latest revision as of 01:43, 2 November 2017

It's cheap. Two orders of magnitude less than alternatives.

And it holds its own against the gold standard - a spectrophotometer.

It's easy to build. A college freshman built it in two hours.

Want to build one yourself? We know you want to.

But designing it wasn't so easy. See how we failed - and tried again.