Difference between revisions of "Team:SHSBNU China"

(Prototype team page)
 
 
(15 intermediate revisions by 3 users not shown)
Line 1: Line 1:
{{SHSBNU_China}}
+
<html lang="zh-CN">
<html>
+
<head>
 +
    <meta http-equiv="Content-Type" content="text/html; charset=UTF-8" />
 +
    <meta name="viewport" content="width=device-width, initial-scale=1" />
  
 +
    <title>SHSBNU-China iGEM 2017 Home Page</title>
 +
<style type="text/css">
 +
        #sideMenu, #top_title {display:none;}
 +
        #content { padding:0px; width:100%; margin-top:0px; margin-left:0px; background-color: #F1F1F1; text-align: center}
  
<div class="column full_size" >
+
    </style>
<img src="http://placehold.it/2000x300/d3d3d3/f2f2f2">
+
</div>
+
  
 +
    <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:SHSBNU_China/CSS1?action=raw&ctype=text/css" />
 +
    <style type="text/css" media="screen">
 +
  html { margin-top: 32px !important; }
 +
  * html body { margin-top: 32px !important; }
 +
  @media screen and ( max-width: 782px ) {
 +
html { margin-top: 46px !important; }
 +
  * html body { margin-top: 46px !important; }
 +
  }
 +
        a {
 +
            opacity: 1;
 +
        }
  
 +
        .scanlines {
 +
            position: absolute;
 +
            top: 0px;
 +
            left: 0px;
 +
            width: 100%;
 +
            height: 100%;
 +
            z-index: 2;)
 +
        }
 +
    </style>
 +
    <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:SHSBNU_China/CSS2?action=raw&ctype=text/css" media="screen" />
 +
    <script type="text/javascript" src="https://2017.igem.org/Template:SHSBNU_China/JS1?action=raw&ctype=text/javascript"></script>
 +
    <script type="text/javascript" src="https://2017.igem.org/Template:SHSBNU_China/JS2?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript">
 +
$(document).ready(function(){  
 +
$('#slideshow').cycle({
 +
        fx:    'fade',
 +
        speed:  'slow',
 +
        timeout: 5000,
 +
        pager:  '#slider_nav',
 +
        pagerAnchorBuilder: function(idx, slide) {
 +
        return '#slider_nav li:eq(' + (idx) + ') a';
 +
        }
 +
    });
 +
});
 +
</script>
  
<div class="column full_size" >
+
</head>
<h1> Welcome to iGEM 2017! </h1>
+
<p>Your team has been approved and you are ready to start the iGEM season! </p>
+
</div>  
+
  
<div class="clear"></div>
+
<body class="home blog logged-in admin-bar no-customize-support" >
 +
    <div style="overflow: hidden; z-index: -1; position: fixed; height: 100%; width: 100%; top: 0px; left: 0px; ">
 +
        <img  id="background_picture" src="https://static.igem.org/mediawiki/2017/e/ee/SHSBNU_17_background.jpg" style="position: fixed; display: block; left: 0px; top: 0px;">
 +
    </div>
  
<div class="column half_size" >
+
    <div id="wrap">
<h5>Before you start: </h5>
+
        <div id="branding">
<p> Please read the following pages:</p>
+
            <a href="https://2017.igem.org/Team:SHSBNU_China"> <img src="https://static.igem.org/mediawiki/2017/a/aa/SHSBNU_17_Banner.jpg" /></a>
<ul>
+
        </div>
<li>  <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
+
<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
+
<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
+
</ul>
+
</div>
+
  
<div class="column half_size" >
+
        <div id="nav">
<div class="highlight">
+
        <nav class = "main" style = "font-size:16px; text-align: center;" >
<h5> Styling your wiki </h5>
+
            <a href="https://2017.igem.org/Team:SHSBNU_China/Project" style = "opacity: 1;">Project</a>
<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
+
            <a> | </a>
<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
+
            <a href="https://2017.igem.org/Team:SHSBNU_China/Model" style = "opacity: 1;">Model</a>
</div>
+
            <a> | </a>
</div>
+
            <a href="https://2017.igem.org/Team:SHSBNU_China/HP" style = "opacity: 1;">HP</a>
 +
            <a> | </a>
 +
            <a href="https://2017.igem.org/Team:SHSBNU_China/Safety" style = "opacity: 1;">Safety</a>
 +
            <a> | </a>
 +
            <a href="https://2017.igem.org/Team:SHSBNU_China/Parts" style = "opacity: 1;">Parts</a>
 +
            <a> | </a>
 +
            <a href="https://2017.igem.org/Team:SHSBNU_China/Team" style = "opacity: 1;">Team</a>
 +
            <a> | </a>
 +
            <a href="https://2017.igem.org/Team:SHSBNU_China/Achievement" style = "opacity: 1;">Achievement</a>
 +
        </nav>
 +
        </div>
  
<div class="column full_size" >
+
        <!--
<h5> Wiki template information </h5>
+
        <div id = "nav">
<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
+
        <nav class = "title" style = "font-size:20px; text-align: center">
 
+
            <p style = "font-family:arial;color:#000;font-size:32px; text-align: center"> Gut Inflammation Dectors </p>
</div>
+
        </nav>
 
+
        </div>
 
+
        -->
 
+
 
+
<div class="column half_size" >
+
<h5> Editing your wiki </h5>
+
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
+
<p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
+
 
+
</div>
+
 
+
 
+
<div class="column half_size" >
+
<h5>Tips</h5>
+
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
+
<ul>
+
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
<li>Be clear about what you are doing and how you plan to do this.</li>
+
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
+
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
+
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
+
<li>Have lots of fun! </li>
+
</ul>
+
</div>
+
 
+
 
+
<div class="column half_size" >
+
<h5>Inspiration</h5>
+
<p> You can also view other team wikis for inspiration! Here are some examples:</p>
+
<ul>
+
<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
+
<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
+
<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
+
<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
+
<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
+
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
+
</ul>
+
</div>
+
 
+
<div class="column half_size" >
+
<h5> Uploading pictures and files </h5>
+
<p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
+
When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
+
 
+
<a href="https://2017.igem.org/Special:Upload">
+
UPLOAD FILES
+
</a>
+
</p>
+
</div>
+
  
 +
      <div id="slider">
  
 +
    <div id="slideshow">
 +
        <div class="slider-item"><a href="https://2017.igem.org/Team:SHSBNU_China/Project"><img src="https://static.igem.org/mediawiki/2017/2/27/SHSBNU_17_Slider1.png" alt="icon" width="1755" height="1242" border="0" /></a></div>
 +
        <div class="slider-item"><a href="https://2017.igem.org/Team:SHSBNU_China/Project"><img src="https://static.igem.org/mediawiki/2017/0/07/SHSBNU_17_Slider2.jpg" alt="icon" width="1755" height="1242" border="0" /></a></div>
 +
        <div class="slider-item"><a href="https://2017.igem.org/Team:SHSBNU_China/Experiment"><img src="https://static.igem.org/mediawiki/2017/7/79/SHSBNU_17_Slider3.jpg" alt="icon" width="1755" height="1242" border="0" /></a></div>
 +
        <div class="slider-item"><a href="https://2017.igem.org/Team:SHSBNU_China/Team/Members"><img src="https://static.igem.org/mediawiki/2017/5/55/SHSBNU_17_Slider4.jpg" alt="icon" width="1755" height="1242" border="0" /></a></div>
 +
    </div>
  
 +
    <div class="controls-center">
 +
        <div id="slider_controls">
 +
        <ul id="slider_nav">
 +
        <li><a href="#"></a></li>
 +
        <li><a href="#"></a></li>
 +
        <li><a href="#"></a></li>
 +
        <li><a href="#"></a></li>
 +
        </ul>
 +
        </div>
 +
    </div>
 +
    <div class="clr"></div>
 +
    </div>
  
 +
        <div class="post-75 post type-post status-publish format-standard hentry category-azir-quantum-science concavebox">
 +
            <div class="entry" style="opacity: 0.95;">
 +
                <p style = "font-family:arial;color:#444444;font-size:32px; text-align: center"> Abstract </p>
 +
                <p> Because thiosulfate and tetrathionate are indicators of intestinal inflammation (Levitt et al, 1999), this system can be used to detect it noninvasively. Although the detailed process and reasons for their production are still unknown, it has been established that the level of thiosulfate and tetrathionate is directly proportional to the seriousness of intestinal inflammation. Until now, scientists were able to detect thiosulfate and tetrathionate using a detector based on a two-component system, which includes two parts: detector and reporter. The detector was derived from a marine Shewanella species, and previous experiments on the detection of intestinal inflammation used sfGFP to show the result. However, we thought this method can be further improved. </p>
  
 +
<p>The SHSBNU_China team worked on changing the reporter part to display the results more clearly and visibly, not requiring specially-produced ultraviolet light or prolonged contact with oxygen. In our system, E. coli would produce a chromo-protein to change its color (even in an anaerobic environment). Furthermore, to enable the result to be observed more easily and to ensure its safety, we planned to produce a pill in which the modified E. coli is stored, with special walls that would only allow small molecules to pass through. In addition to the conventional chromo-proteins it can produce, we designed and produced an additional plasmid using violacein as the reporter. Violacein was successfully produced and the corresponding strain showed obvious purple color under anaerobic conditions. The special reason for choosing violacein is that this compound can cure or at least slow down the inflammation to some degree. </p>
 +
 +
<p>The two-component system detector used in our project has the potential to be further modified to produce additional treatments upon detecting inflammation. We also came up with solutions to reduce potential risks in practical treatments. For example, we have discussed creating kill switches, using DNase to destroy the engineered gene to prevent its spread into the normal gut flora.
 +
</p>
 +
            </div><!-- .entry -->
  
 +
            <div class="entry-footer" align = "center">
 +
                <a href="http://www.shsbnu.net" style = "float:left"><img src = "https://static.igem.org/mediawiki/2017/4/44/SHSBNU_Logo_256X256.jpg" style="height:48px; width:48px; " border=0></a>
 +
                <a href="http://www.azirquantum.net.cn" style = "float:left"><img src = "https://static.igem.org/mediawiki/2017/1/1f/Azir_Logo_256X256.jpg" style="height:48px; width:48px; " border=0></a>
 +
                <a href="https://2017.igem.org" style = "float:right"><img src = "https://static.igem.org/mediawiki/2017/6/60/IGEM_Logo_256X256.jpg" style="height:48px; width:48px; " border=0></a>
 +
                <a href="http://www.bluepha.com" style = "float:right"><img src = "https://static.igem.org/mediawiki/2017/b/bc/Bluepha_Logo_256X256.jpg" style="height:48px; width:48px; " border=0></a>
 +
                <P style = "font-family:arial;color:#5C5C5C;font-size:10px; text-align: center">iGEM 2017</P>
 +
                <P style = "font-family:arial;color:#5C5C5C;font-size:10px; text-align: center">Made by Azir.</P>
 +
                <p style = "font-family:arial;color:#5C5C5C;font-size:10px; text-align: center">The Second High School Attached Beijing Normal University International Division </p>
 +
            </div>
  
 +
    </div><!-- .concavebox -->
 +
</body>
 
</html>
 
</html>

Latest revision as of 02:14, 2 November 2017

SHSBNU-China iGEM 2017 Home Page

icon
icon
icon
icon

Abstract

Because thiosulfate and tetrathionate are indicators of intestinal inflammation (Levitt et al, 1999), this system can be used to detect it noninvasively. Although the detailed process and reasons for their production are still unknown, it has been established that the level of thiosulfate and tetrathionate is directly proportional to the seriousness of intestinal inflammation. Until now, scientists were able to detect thiosulfate and tetrathionate using a detector based on a two-component system, which includes two parts: detector and reporter. The detector was derived from a marine Shewanella species, and previous experiments on the detection of intestinal inflammation used sfGFP to show the result. However, we thought this method can be further improved.

The SHSBNU_China team worked on changing the reporter part to display the results more clearly and visibly, not requiring specially-produced ultraviolet light or prolonged contact with oxygen. In our system, E. coli would produce a chromo-protein to change its color (even in an anaerobic environment). Furthermore, to enable the result to be observed more easily and to ensure its safety, we planned to produce a pill in which the modified E. coli is stored, with special walls that would only allow small molecules to pass through. In addition to the conventional chromo-proteins it can produce, we designed and produced an additional plasmid using violacein as the reporter. Violacein was successfully produced and the corresponding strain showed obvious purple color under anaerobic conditions. The special reason for choosing violacein is that this compound can cure or at least slow down the inflammation to some degree.

The two-component system detector used in our project has the potential to be further modified to produce additional treatments upon detecting inflammation. We also came up with solutions to reduce potential risks in practical treatments. For example, we have discussed creating kill switches, using DNase to destroy the engineered gene to prevent its spread into the normal gut flora.