Difference between revisions of "Team:UCL/InterLab"

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<h3>★  ALERT! </h3>
 
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
 
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
 
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<h1>InterLab</h1>
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    <title>Interlab</title>
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<h3>Bronze Medal Criterion #4</h3>
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range.
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For teams participating in the <a href="https://2017.igem.org/Competition/InterLab_Study">InterLab study</a>, all work must be shown on this page.  
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      <a href="https://2017.igem.org/Team:UCL"><img src="https://static.igem.org/mediawiki/2017/9/92/Logotop.png" class="logo-top"></a>
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            <li id="drop1" class="navy-link"><a href="https://2017.igem.org/Team:UCL/Description">PROJECT</a>
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                    <li><a href="https://2017.igem.org/Team:UCL/Description" class="dropped">Description</a></li>
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                    <li><a href="https://2017.igem.org/Team:UCL/Results" class="dropped">Results</a></li>
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                    <li><a href="https://2017.igem.org/Team:UCL/Parts" class="dropped">Basic Parts</a></li>
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                    <li><a href="https://2017.igem.org/Team:UCL/Part_Improvement" class="dropped">Gold Part</a></li>
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                    <li><a href="https://2017.igem.org/Team:UCL/InterLab" class="dropped">Interlab</a></li>
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            <li id="drop" class="navy-link"><a href="https://2017.igem.org/Team:UCL/Human_Practices">HUMAN PRACTICES</a>
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                    <li><a href="https://2017.igem.org/Team:UCL/Human_Practices" class="dropped">Overview</a></li>
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                    <li><a href="https://2017.igem.org/Team:UCL/HP/Silver" class="dropped">HP Silver</a></li>
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                    <li><a href="https://2017.igem.org/Team:UCL/Model2" class="dropped">Optoflux</a></li>
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            <li id="drop3" class="navy-link"><a href="https://2017.igem.org/Team:UCL/Entrepreneurship">ENTREPRENEURSHIP</a>
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            <li id="drop2" class="navy-link"><a href="https://2017.igem.org/Team:UCL/Medals">OUR MEDALS</a></li>
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            <li id="drop6" class="navy-link"><a href="#about">PEOPLE</a>
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            <li id="drop4" class="navy-link"><a href="https://2017.igem.org/Team:UCL/Notebook">LAB BOOK</a>
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            <div style="margin-top:20px"><h1 class="title" style="font-size: 50px!important; color: #E55190; margin-left:0">Interlab</h1></div>
  
  
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                <p class="text-content-new">With the multipurpose applications of our light induced technologies comes the consideration for reproducibility and reliability in different environments.</p>
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                <p class="text-content-new">The aim of this year’s Interlab study was to analyse how bacterial cell culture optical density (OD) and green fluorescent protein (GFP) fluorescence measurements differ with a plate reader or flow cytometer across labs around the world. This will ultimately help to establish a reliable GFP measurement protocol that overcomes the variations observed between different measurement systems.  Therefore, this study contributes to improving the reproducibility and reliability in synthetic biology.</p>
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                <p class="text-content-new">All protocols and methods followed were provided by iGEM, each of which can be found at:</p>
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<ul class=“text-content-new” style="margin-left:90px!important; padding-left:90px!important">
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                  <li class="text-hyperlink" style=“margin-left: 40px!important; text-decoration:none”><a href="http://parts.igem.org/Help:2017_Competent_Cell_Test_Kit">Competency test</a></li>
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                  <li class="text-hyperlink" style=“margin-left: 40px!important; text-decoration:none”><a href="http://parts.igem.org/Help:Protocols/Transformation">Transformation (single tube)</a></li>
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                  <li  class="text-hyperlink"style=“margin-left: 40px!important; text-decoration:none”><a href="https://static.igem.org/mediawiki/2017/8/85/InterLab_2017_Plate_Reader_Protocol.pdf">Plate Reader measurements</a></li>
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                  </ul>
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                <p class="text-content-new">Our results with the plate reader has been submitted to iGEM, but for those curious, our results can also be downloaded from this link:<a href="https://static.igem.org/mediawiki/2017/f/fd/UCL_InterLab_2017_Measurements.xls.xlsx"class="text-hyperlink"> UCL Interlab Measurement Data</a></p>
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                <p class="text-content-new">We have fully enjoyed participating in the Interlab study and hope to have successfully contributed to this global endeavour.</p>
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<img src="https://static.igem.org/mediawiki/2017/1/17/19942865_1361198747297840_177833111941434553_o.jpg" class="image-content-graphs" style="width:80%; height: 50%">
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Latest revision as of 02:21, 2 November 2017

Interlab

Interlab

With the multipurpose applications of our light induced technologies comes the consideration for reproducibility and reliability in different environments.

The aim of this year’s Interlab study was to analyse how bacterial cell culture optical density (OD) and green fluorescent protein (GFP) fluorescence measurements differ with a plate reader or flow cytometer across labs around the world. This will ultimately help to establish a reliable GFP measurement protocol that overcomes the variations observed between different measurement systems. Therefore, this study contributes to improving the reproducibility and reliability in synthetic biology.

All protocols and methods followed were provided by iGEM, each of which can be found at:

Our results with the plate reader has been submitted to iGEM, but for those curious, our results can also be downloaded from this link: UCL Interlab Measurement Data

We have fully enjoyed participating in the Interlab study and hope to have successfully contributed to this global endeavour.

Back to Top