Difference between revisions of "Team:Uppsala/Demonstrate"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Demonstrate</h1>
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<h3>Gold Medal Criterion #4</h3>
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
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Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
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    <img src="https://static.igem.org/mediawiki/2017/9/94/Uppsala-new-Demonstrate-header.svg" style="width:100%; height: auto">
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      <div class="header" style="margin-top:100px;">Demonstrate</div>
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      <div style="padding-right:3%; padding-left:3%; text-align:justify;"><br>We successfully integrated the first five steps of the crocin pathway from FPP to zeaxanthin into the E. coli chromosome. The result is a <i>E. coli</i> strain expressing zeaxanthin. We have created sequence verified BioBricks of our enzymes in the extended crocin pathway: CaCCD2, CsADH2946 and UGTCs2. We have also characterized these enzymes with experiments and simulations. We are the first to purify and confirm activity of CsADH2946 as well as measuring the kinetic parameters of the enzyme. We created and combined the zeaxanthin producing strain with a plasmid containing the extended crocin pathway which gave us an <i>E. coli</i> strain including the entire production pathway from FPP to crocin.</div>
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<h4> What should we do for our demonstration?</h4>
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<h5> Standard teams </h5>
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If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
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<h5> Special track teams </h5>
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<div style="padding-right:3%; padding-left:3%; text-align:justify;">Detailed results that show that our project works can be found on our <a href= "https://2017.igem.org/Team:Uppsala/Results"> Results page </a></di>
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Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
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<img src="https://static.igem.org/mediawiki/2017/0/0d/Uppsala-ZeaBottle.png" alt="Plate with colonies" style="width:30%;margin:auto;"></img>
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<figcaption class="figure-caption figtext" style="padding-bottom: 2%; padding-left: 20%; padding-right: 20%;">
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<i>E. coli </i> culture producing colorful pigments from the crocin pathway.
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Latest revision as of 02:30, 2 November 2017

<!DOCTYPE html> Results

Demonstrate

We successfully integrated the first five steps of the crocin pathway from FPP to zeaxanthin into the E. coli chromosome. The result is a E. coli strain expressing zeaxanthin. We have created sequence verified BioBricks of our enzymes in the extended crocin pathway: CaCCD2, CsADH2946 and UGTCs2. We have also characterized these enzymes with experiments and simulations. We are the first to purify and confirm activity of CsADH2946 as well as measuring the kinetic parameters of the enzyme. We created and combined the zeaxanthin producing strain with a plasmid containing the extended crocin pathway which gave us an E. coli strain including the entire production pathway from FPP to crocin.

Detailed results that show that our project works can be found on our Results page

Plate with colonies
E. coli culture producing colorful pigments from the crocin pathway.