Difference between revisions of "Team:TNCR Korea/Part Collection"

 
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{{TNCR_Korea}}
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<h1> Part Collection </h1>
 
  
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<ul>
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<!-- Modernizr JS -->
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    <li>BBa_K2496000- FLAG</li>
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<script src="https://2017.igem.org/Template:TNCR_Korea_modernizr/Javascript?
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<li>BBa_K2496001 = DPP4</li>
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action=raw&ctype=text/javascript"></script>
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<li>BBa_K2496002= strong recombinant </li>
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<!-- FOR IE9 below -->
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<li>BBa_K2496003= strong anderson promoter(BBa_J23110)</li>
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<!--[if lt IE 9]>
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<li>BBa_K2496004= moderate anderson promoter(BBa_J23111)</li>
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<script src="https://2017.igem.org/Template:TNCR_Korea_respond/Javascript?
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<li>BBa_K2496005= weak anderson promoter(BBa_J23116)</li>
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action=raw&ctype=text/javascript"></script>
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<li>BBa_K2496006= moderate recombinant</li>
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<![endif]-->
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<li>BBa_K2496007= weak recombinant</li>
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</ul>
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<style>
  
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<p>Three composite plasmid(strong, moderate, weak) was designed to control the expression of DPP4, because DPP4 can induce inflammation.
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/***************************************************** DEFAULT WIKI SETTINGS  ****************************************************/
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Strong recombinant plasmid have most strong expression(1) of DPP4
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Moderate recombinant plasmid have moderate expression(0.58) of DPP4
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/* Clear the default wiki settings */
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Weak recombinant plasmid have most weak expression(0.1) of DPP4</p>
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<p>TNCR_Korea constituted a total of three recombinant plasmids. Anderson promoter (strong, moderate, weak.) + FLAG + DPP4. There are two Pst1 sites in the DPP4 sequence, so you can not access the desired 3A assembly method from iGEM. So we used infusion ligation method instead.</p>
 
  
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<h1 id="fh5co-logo"><a href="https://2017.igem.org/Team:TNCR_Korea"><span>TNCR</span>KOREA</a></h1>
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<li><a href="https://2017.igem.org/Team:TNCR_Korea/Team" target="_blank">Team</a></li>
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<li><a href="https://2017.igem.org/Team:TNCR_Korea/Attributions" target="_blank">Attributions</a></li>
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<li><a href="https://2017.igem.org/Team:TNCR_Korea/Collaborations" target="_blank">Collaborations</a></li>
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<li><a href="https://2017.igem.org/Team:TNCR_Korea/Background" target="_blank">Background</a></li>
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<li><a href="https://2017.igem.org/Team:TNCR_Korea/Description" target="_blank">Description</a></li>
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<li><a href="https://2017.igem.org/Team:TNCR_Korea/Design" target="_blank">Design</a></li>
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<li><a href="https://2017.igem.org/Team:TNCR_Korea/Model" target="_blank">Model</a></li>
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<li><a href="https://2017.igem.org/Team:TNCR_Korea/Experiments" target="_blank">Experiments</a></li>
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<li><a href="https://2017.igem.org/Team:TNCR_Korea/Notebook" target="_blank">Notebook</a></li>
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<li><a href="https://2017.igem.org/Team:TNCR_Korea/Parts" target="_blank">Parts</a></li>
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<li><a href="https://2017.igem.org/Team:TNCR_Korea/Contribution" target="_blank">Contribution</a></li>
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<li><a href="https://2017.igem.org/Team:TNCR_Korea/Basic_Part" target="_blank">Basic Part</a></li>
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<li><a href="https://2017.igem.org/Team:TNCR_Korea/Composite_Part" target="_blank">Composite Part</a></li>
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<li><a href="https://2017.igem.org/Team:TNCR_Korea/Part_Collection" target="_blank">Part Collection</a></li>
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<li>
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<a href="portfolio.html" class="fh5co-sub-ddown">Human Practices</a>
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<li><a href="https://2017.igem.org/Team:TNCR_Korea/HP/Silver" target="_blank">Human Activity</a></li>
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<li><a href="https://2017.igem.org/Team:TNCR_Korea/HP/Gold_Integrated" target="_blank">Integrated Human Activity</a></li>
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<li><a href="https://2017.igem.org/Team:TNCR_Korea/Engagement" target="_blank">Public Engagement</a></li>
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<a href="https://2017.igem.org/Team:TNCR_Korea/Awards">Awards</a>
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<div class="desc animate-box">
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<h2>Our Parts</h2>
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<span>---Part Collection---</span>
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</div>
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</div>
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</div>
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<!-- end:header-top -->
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<h3>Our Part Collection</h3>
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<div class="col-lg-4 col-md-4">
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496000"><img class="img-responsive" src="https://i.imgur.com/zfZIZJS.png" alt=""></a>
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<div class="image-popup" href="#">
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<h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496000"#>BBa_K2496000</a></h3>
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<p> = FLAG</p>
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</div>
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<div class="col-lg-4 col-md-4">
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496001"><img class="img-responsive" src="https://i.imgur.com/HmeqmpR.png" alt=""></a>
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<div class="image-popup" href="#">
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<h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496001"#>BBa_K2496001</a></h3>
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<p>= DPP4</p>
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<div class="col-lg-4 col-md-4">
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496002"><img class="img-responsive" src="https://i.imgur.com/XcIJE1C.png" alt=""></a>
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<div class="image-popup" href="#">
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<div class="prod-title">
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<h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496002"#>BBa_K2496002</a></h3>
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<p>= strong recombinant</p>
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</div>
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</div>
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</div>
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<div class="col-lg-4 col-md-4">
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<div class="fh5co-blog animate-box">
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496003"><img class="img-responsive" src="https://i.imgur.com/jLsYpEH.png" alt=""></a>
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<div class="image-popup" href="#">
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<div class="prod-title">
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<h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496003"#>BBa_K2496003</a></h3>
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<p>= strong anderson promoter(BBa_J23110)</p>
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</div>
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</div>
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</div>
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</div>
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<div class="col-lg-4 col-md-4">
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<div class="fh5co-blog animate-box">
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496004"><img class="img-responsive" src="https://i.imgur.com/kBG2Spz.png" alt=""></a>
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<div class="image-popup" href="#">
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<div class="prod-title">
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<h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496004"#>BBa_K2496004</a></h3>
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<p>= moderate anderson promoter(BBa_J23111)</p>
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</div>
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</div>
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</div>
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</div>
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<div class="col-lg-4 col-md-4">
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<div class="fh5co-blog animate-box">
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496005"><img class="img-responsive" src="https://i.imgur.com/9XjWM1c.png" alt=""></a>
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<div class="image-popup" href="#">
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<div class="prod-title">
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<h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496005"#>BBa_K2496005</a></h3>
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<p>= weak anderson promoter(BBa_J23116)</p>
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</div>
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</div>
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</div>
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</div>
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<div class="col-lg-4 col-md-4">
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<div class="fh5co-blog animate-box">
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496006"><img class="img-responsive" src="https://i.imgur.com/V6fpQTT.png" alt=""></a>
 +
<div class="image-popup" href="#">
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<div class="prod-title">
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<h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496006"#>BBa_K2496006</a></h3>
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<p>= moderate recombinant</p>
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</div>
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</div>
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</div>
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<div class="col-lg-4 col-md-4">
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<div class="fh5co-blog animate-box">
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496007"><img class="img-responsive" src="https://i.imgur.com/0POyQ57.jpg" alt=""></a>
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<div class="image-popup" href="#">
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<div class="prod-title">
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<h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2496007"#>BBa_K2496007</a></h3>
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<p>= weak recombinant</p>
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</div>
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<div class="col-md-8 col-md-push-1">
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<h4 class="fh5co-number">Comment</h4>
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<h2>Part Collection</h2>
 +
<p>To achieve our goal, TNCR_Korea wanted to incorporate DPP4 gene. However, DPP4 may trigger the inflammation response in case. Therefore, we needed to control its expression. TNCR_Korea team adapted the previous BioBrick Parts, named Anderson promoter (BBa_J23110, BBa_J23111, BBa_J23116), then designed three composite plasmids (strong BBa_K2496002, moderate BBa_K2496006, weak BBa_K2496007). Basically, DPP4 was inserted with Flag molecular tag for easy detection of its incorporation by checking tag’s expression (DPP4 BBa_K2496001 and Flag BBa_K2496000). There are two Pst1 sites in the DPP4 sequence, so it is not possible to access the desired 3A assembly method from iGEM. So we used infusion ligation method instead. Consequently, TNCR_Korea constituted recombinant plasmid which has Anderson promoter, DPP4, and Flag. The Range of Part Numbers are, 1) Best New Basic part: BBa_K2496000 (Flag) BBa_K2496001 (DPP4), 2) Best New composite part: BBa_K2496002 (strong), BBa_K2496006 (moderate), BBa_K2496007 (weak), 3) Best part collection ranged from BBa_K2496000 to BBa_K2496007.</p>
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Latest revision as of 02:38, 2 November 2017

TNCR_KOREA — Make gluten digestable

Our Parts

---Part Collection---

Our Part Collection

BBa_K2496000

= FLAG

BBa_K2496001

= DPP4

BBa_K2496002

= strong recombinant

BBa_K2496003

= strong anderson promoter(BBa_J23110)

BBa_K2496004

= moderate anderson promoter(BBa_J23111)

BBa_K2496005

= weak anderson promoter(BBa_J23116)

BBa_K2496006

= moderate recombinant

BBa_K2496007

= weak recombinant

Comment

Part Collection

To achieve our goal, TNCR_Korea wanted to incorporate DPP4 gene. However, DPP4 may trigger the inflammation response in case. Therefore, we needed to control its expression. TNCR_Korea team adapted the previous BioBrick Parts, named Anderson promoter (BBa_J23110, BBa_J23111, BBa_J23116), then designed three composite plasmids (strong BBa_K2496002, moderate BBa_K2496006, weak BBa_K2496007). Basically, DPP4 was inserted with Flag molecular tag for easy detection of its incorporation by checking tag’s expression (DPP4 BBa_K2496001 and Flag BBa_K2496000). There are two Pst1 sites in the DPP4 sequence, so it is not possible to access the desired 3A assembly method from iGEM. So we used infusion ligation method instead. Consequently, TNCR_Korea constituted recombinant plasmid which has Anderson promoter, DPP4, and Flag. The Range of Part Numbers are, 1) Best New Basic part: BBa_K2496000 (Flag) BBa_K2496001 (DPP4), 2) Best New composite part: BBa_K2496002 (strong), BBa_K2496006 (moderate), BBa_K2496007 (weak), 3) Best part collection ranged from BBa_K2496000 to BBa_K2496007.