Difference between revisions of "Team:Gaston Day School/Notebook"

 
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         margin-left: 40px;
 
         margin-left: 40px;
 
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         margin-right: 40px;
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        margin-top: 40px;
 
         }
 
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.pic{
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    height: 640px;
 +
    width: 480px;
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    margin-left: 500px;
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</style>
 
</style>
  
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−
<div class="panel panel-default">
+
<div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
 +
            <div class="panel panel-default">
 
                 <div class="panel-heading" role="tab" id="P1">
 
                 <div class="panel-heading" role="tab" id="P1">
 
                     <h4 class="panel-title">
 
                     <h4 class="panel-title">
 
                     <a role="button" data-toggle="collapse" data-parent="#accordion" href="#P1-collapse" aria-expanded="false" aria-controls="P1-collapse">
 
                     <a role="button" data-toggle="collapse" data-parent="#accordion" href="#P1-collapse" aria-expanded="false" aria-controls="P1-collapse">
 
<div>
 
<div>
−
                     <div class="col-md-11">09/01/2017</div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div>
+
                     <div class="col-md-11">09/01/2017 PCR Primer</div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div>
 
</div>
 
</div>
 
                     </a>
 
                     </a>
Line 42: Line 49:
 
                 <div id="P1-collapse" class="panel-collapse collapse" role="tabpanel" aria-labelledby="P1">
 
                 <div id="P1-collapse" class="panel-collapse collapse" role="tabpanel" aria-labelledby="P1">
 
                     <div class="panel-body">
 
                     <div class="panel-body">
−
<p>
+
<p>Master Mix:</p>
−
<b>Materials:</b><br>
+
<table class="table table-bordered table-striped">
−
LB broth<br>
+
    <tbody>
−
Ice<br>
+
        <tr>
−
Selection plates<br>
+
            <td>14.3</td>
−
<br>
+
            <td>H2O</td>
−
<b>Methods:</b><br>
+
            <td>114.4</td>
−
<ol style="font-size:16px;">
+
        </tr>
−
<li>Thaw 50µL competent E. coli cells on ice for 10 minutes<br></li>
+
        <tr>
−
<li>Add:
+
            <td>2.5</td>
−
<ul style="font-size:16px;">
+
            <td>Buffer</td>
−
<li>5-10 µl DNA from a ligation reaction mix or </li>
+
            <td>20</td>
−
<li>10-100ng DNA of a known plasmid </li>
+
        </tr>
−
</ul>
+
        <tr>
−
</li>
+
            <td>1</td>
−
<li>Carefully flick the tube 4-5 times to mix cells and DNA. <b>Do not vortex.</b></li>
+
            <td>MgCl2</td>
−
<li>Place the mixture on ice for 30 minutes. <b>Do not mix.</b></li>
+
            <td>8</td>
−
<li>Heat shock at exactly 42°C for exactly 30 seconds. <b>Do not mix.</b></li>
+
        </tr>
−
<li>Place on ice for 5 minutes. <b>Do not mix.</b></li>
+
        <tr>
−
<li>Pipette 950 µl of room temperature SOC or LB media into the mixture.</li>
+
            <td>1</td>
−
<li>Incubate at 37°C and 200-250 rpm for 60 minutes.</li>
+
            <td>dNTP</td>
−
<li>Mix the cells thoroughly by flicking the tube and inverting.</li>
+
            <td>8</td>
−
<li>Spread:
+
        </tr>
−
<ul style="font-size:16px;">
+
    </tbody>
−
<li>For ligation reaction DNA: 100µl of each transformation reaction onto a selection plate. For the rest of 900 µL:
+
</table>
−
<ol style="font-size:16px;">
+
<p>tube:</p>
−
<li>Pellet cells at 8000rpm for 3 minutes</li>
+
<table class="table table-bordered table-striped">
−
<li>Remove and dispense 600 µL of supernatant </li>
+
  <tbody>
−
<li>Re-suspend cells by light vortexing</li>
+
        <tr>
−
<li>Plate resuspended cells as above</li>
+
            <td>2ul</td>
−
</ol></li>
+
            <td>H2O</td>
 +
        </tr>
 +
        <tr>
 +
            <td>-5ul</td>
 +
            <td>F</td>
 +
        </tr>
 +
        <tr>
 +
            <td>-5ul</td>
 +
            <td>R</td>
 +
        </tr>
 +
  </tbody>
 +
</table>
 +
<p>Label:</p>
 +
<table class="table table-bordered table-striped">
 +
  <tbody>
 +
        <tr>
 +
            <td>0</td>
 +
            <td>Control</td>
 +
        </tr>
 +
        <tr>
 +
            <td>1</td>
 +
            <td>AceF</td>
 +
        </tr>
 +
        <tr>
 +
            <td>2</td>
 +
            <td>EutG BB</td>
 +
        </tr>
 +
        <tr>
 +
            <td>3</td>
 +
            <td>YiaY BB</td>
 +
        </tr>
 +
        <tr>
 +
            <td>4</td>
 +
            <td>AdhP BB</td>
 +
        </tr>
 +
        <tr>
 +
            <td>5</td>
 +
            <td>Lpd</td>
 +
        </tr>
 +
        <tr>
 +
            <td>6</td>
 +
            <td>AceE</td>
 +
        </tr>
 +
  </tbody>
 +
</table>
 +
  </div>
 +
</div>
 +
</div>
  
−
<li>For known plasmid: 10 & 100 µL of each transformation reaction onto a selection plate. For the rest of 890 µL:
 
−
<ol style="font-size:16px;">
 
−
<li>Pellet cells at 8000rpm for 3 minutes</li>
 
−
<li>Remove and dispense 600 µL of supernatant </li>
 
−
<li>Re-suspend cells by light vortexing</li>
 
−
<li>Plate resuspended cells as above</li>
 
−
</ol></li>
 
−
</ul>
 
−
<li>Incubate overnight at 37°C with plates upside down.</li>
 
−
</ol>
 
  
−
</p>
+
 
−
    </div>
+
<div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
−
  </div>
+
            <div class="panel panel-default">
 +
                <div class="panel-heading" role="tab" id="P2">
 +
                    <h4 class="panel-title">
 +
                    <a role="button" data-toggle="collapse" data-parent="#accordion" href="#P2-collapse" aria-expanded="false" aria-controls="P2-collapse">
 +
<div>
 +
                    <div class="col-md-11">09/06/2017 Genomic PCR</div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div>
 
</div>
 
</div>
 +
                    </a>
 +
                    </h4>
  
 +
                </div>
 +
                <div id="P2-collapse" class="panel-collapse collapse" role="tabpanel" aria-labelledby="P2">
 +
                    <div class="panel-body">
 +
<p>Master Mix</p>
 +
<table class="table table-bordered table-striped">
 +
  <tbody>
 +
    <tr>
 +
      <td>gDNA</td>
 +
      <td>3</td>
 +
      <td>24</td>
 +
    </tr>
 +
    <tr>
 +
      <td>Buffer</td>
 +
      <td>2.5</td>
 +
      <td>20</td>
 +
    </tr>
 +
    <tr>
 +
      <td>dNTP</td>
 +
      <td>1</td>
 +
      <td>8</td>
 +
    </tr>
 +
    <tr>
 +
      <td>dH2O</td>
 +
      <td>13.3</td>
 +
      <td>106.4</td>
 +
    </tr>
 +
    <tr>
 +
      <td>Taq</td>
 +
      <td>0.2</td>
 +
      <td>1.6</td>
 +
    </tr>
 +
  </tbody>
 +
</table>
 +
<table class="table table-bordered table-striped">
 +
<thead>
 +
        <tr>
 +
            <th></th>
 +
            <th>dH2O</th>
 +
            <th>For</th>
 +
            <th>Rev</th>
 +
            <th></th>
 +
        </tr>
 +
    </thead>
 +
    <tbody>
 +
        <tr>
 +
            <td>1</td>
 +
            <td>4</td>
 +
            <td>0.5</td>
 +
            <td>0.5</td>
 +
            <td>AceF<i class="fa fa-check fa-2x fa-fw" ></i></td>
 +
        </tr>
 +
        <tr>
 +
            <td>2</td>
 +
            <td>4</td>
 +
            <td>0.5</td>
 +
            <td>0.5</td>
 +
            <td>EutG<i class="fa fa-check fa-2x fa-fw" ></i></td>
 +
        </tr>
 +
        <tr>
 +
            <td>3</td>
 +
            <td>4</td>
 +
            <td>0.5</td>
 +
            <td>0.5</td>
 +
            <td>YiaY<i class="fa fa-check fa-2x fa-fw" ></i></td>
 +
        </tr>
 +
        <tr>
 +
            <td>4</td>
 +
            <td>4</td>
 +
            <td>0.5</td>
 +
            <td>0.5</td>
 +
            <td>AdnP<i class="fa fa-check fa-2x fa-fw" ></i></td>
 +
        </tr>
 +
        <tr>
 +
            <td>5</td>
 +
            <td>4</td>
 +
            <td>0.5</td>
 +
            <td>0.5</td>
 +
            <td>Lpd</td>
 +
        </tr>
 +
        <tr>
 +
            <td>6</td>
 +
            <td>4</td>
 +
            <td>0.5</td>
 +
            <td>0.5</td>
 +
            <td>AceE</td>
 +
        </tr>
 +
        <tr>
 +
            <td>7</td>
 +
            <td>5</td>
 +
            <td>-</td>
 +
            <td>-</td>
 +
            <td>-</td>
 +
        </tr>
 +
    </tbody>
 +
</table>
 +
</div>
 +
</div>
 +
</div>
  
−
</body>
 
  
 +
<div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
 +
            <div class="panel panel-default">
 +
                <div class="panel-heading" role="tab" id="P3">
 +
                    <h4 class="panel-title">
 +
                    <a role="button" data-toggle="collapse" data-parent="#accordion" href="#P3-collapse" aria-expanded="false" aria-controls="P3-collapse">
 +
<div>
 +
                    <div class="col-md-11">09/11/2017 Genomic PCR</div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div>
 +
</div>
 +
                    </a>
 +
                    </h4>
  
 +
                </div>
 +
                <div id="P3-collapse" class="panel-collapse collapse" role="tabpanel" aria-labelledby="P3">
 +
                    <div class="panel-body">
 +
<p>Master Mix</p>
 +
<table class="table table-bordered table-striped">
 +
  <tbody>
 +
    <tr>
 +
      <td>gDNA</td>
 +
      <td>3</td>
 +
      <td>24</td>
 +
    </tr>
 +
    <tr>
 +
      <td>Buffer</td>
 +
      <td>2.5</td>
 +
      <td>20</td>
 +
    </tr>
 +
    <tr>
 +
      <td>dNTP</td>
 +
      <td>1</td>
 +
      <td>8</td>
 +
    </tr>
 +
    <tr>
 +
      <td>dH2O</td>
 +
      <td>12.3</td>
 +
      <td>98.4</td>
 +
    </tr>
 +
    <tr>
 +
      <td>Taq</td>
 +
      <td>0.2</td>
 +
      <td>1.6</td>
 +
    </tr>
 +
    <tr>
 +
      <td>MgCl2</td>
 +
      <td>1</td>
 +
      <td>8</td>
 +
    </tr>
 +
  </tbody>
 +
</table>
 +
<table class="table table-bordered table-striped">
 +
<thead>
 +
        <tr>
 +
            <th></th>
 +
            <th>dH2O</th>
 +
            <th>For</th>
 +
            <th>Rev</th>
 +
            <th></th>
 +
        </tr>
 +
    </thead>
 +
<tbody>
 +
    <tr>
 +
      <td>0</td>
 +
      <td>5</td>
 +
      <td>-</td>
 +
      <td>-</td>
 +
      <td></td>
 +
    </tr>
 +
    <tr>
 +
      <td>1</td>
 +
      <td>4</td>
 +
      <td>0.5</td>
 +
      <td>0.5</td>
 +
      <td>ACE F</td>
 +
    </tr>
 +
    <tr>
 +
      <td>2</td>
 +
      <td>4</td>
 +
      <td>0.5</td>
 +
      <td>0.5</td>
 +
      <td>EutG</td>
 +
    </tr>
 +
    <tr>
 +
      <td>3</td>
 +
      <td>4</td>
 +
      <td>0.5</td>
 +
      <td>0.5</td>
 +
      <td>YiaY</td>
 +
    </tr>
 +
    <tr>
 +
      <td>4</td>
 +
      <td>4</td>
 +
      <td>0.5</td>
 +
      <td>0.5</td>
 +
      <td>AdhP</td>
 +
    </tr>
 +
    <tr>
 +
      <td>5</td>
 +
      <td>4</td>
 +
      <td>0.5</td>
 +
      <td>0.5</td>
 +
      <td>Lpd</td>
 +
    </tr>
 +
    <tr>
 +
      <td>6</td>
 +
      <td>4</td>
 +
      <td>0.5</td>
 +
      <td>0.5</td>
 +
      <td>AceE</td>
 +
    </tr>
 +
  </tbody>
 +
</table>
 +
</div>
 +
</div>
 +
</div>
  
  
 +
<div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
 +
            <div class="panel panel-default">
 +
                <div class="panel-heading" role="tab" id="P4">
 +
                    <h4 class="panel-title">
 +
                    <a role="button" data-toggle="collapse" data-parent="#accordion" href="#P4-collapse" aria-expanded="false" aria-controls="P4-collapse">
 +
<div>
 +
                    <div class="col-md-11">09/18/2017 PCR check of J23102+GlmY</div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div>
 +
</div>
 +
                    </a>
 +
                    </h4>
  
 +
                </div>
 +
                <div id="P4-collapse" class="panel-collapse collapse" role="tabpanel" aria-labelledby="P4">
 +
                    <div class="panel-body">
 +
<table class="table table-bordered table-striped">
 +
  <thead>
 +
    <th>1</th>
 +
    <th>-</th>
 +
    <th>DNA(0)</th>
 +
    <th>dH2O(5)</th>
 +
    </tr>
 +
  </thead>
 +
  <tbody>
 +
    <tr>
 +
      <td>2</td>
 +
      <td>J23102</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>3</td>
 +
      <td>GlmY</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>4</td>
 +
      <td>A(1)</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>5/td>
 +
      <td>A(2)</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>6</td>
 +
      <td>B(2)</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>7</td>
 +
      <td>C(2)</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
  </tbody>
 +
</table>
 +
<p>Max:</p>
 +
<table class="table table-bordered table-striped">
 +
  <tbody>
 +
    <tr>
 +
      <td>Buffer</td>
 +
      <td>2.5</td>
 +
      <td>20</td>
 +
    </tr>
 +
    <tr>
 +
      <td>dNTP</td>
 +
      <td>1</td>
 +
      <td>8</td>
 +
    </tr>
 +
    <tr>
 +
      <td>VR</td>
 +
      <td>0.5</td>
 +
      <td>4</td>
 +
    </tr>
 +
    <tr>
 +
      <td>VF2</td>
 +
      <td>0.5</td>
 +
      <td>4</td>
 +
    </tr>
 +
    <tr>
 +
      <td>Taq</td>
 +
      <td>0.2</td>
 +
      <td>1.6</td>
 +
    </tr>
 +
    <tr>
 +
      <td>dH2O</td>
 +
      <td>15.3</td>
 +
      <td>122.4</td>
 +
    </tr>
 +
  </tbody>
 +
</table>
 +
</div>
 +
</div>
 +
</div>
 +
 +
 +
<div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
 +
            <div class="panel panel-default">
 +
                <div class="panel-heading" role="tab" id="P3">
 +
                    <h4 class="panel-title">
 +
                    <a role="button" data-toggle="collapse" data-parent="#accordion" href="#P3-collapse" aria-expanded="false" aria-controls="P3-collapse">
 +
<div>
 +
                    <div class="col-md-11">09/25/2017 PCR check for J23102+GlmY</div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div>
 +
</div>
 +
                    </a>
 +
                    </h4>
 +
 +
                </div>
 +
                <div id="P3-collapse" class="panel-collapse collapse" role="tabpanel" aria-labelledby="P3">
 +
                    <div class="panel-body">
 +
<p>Master Mix</p>
 +
<table class="table table-bordered table-striped">
 +
  <tbody>
 +
    <tr>
 +
      <td>Buffer</td>
 +
      <td>2.5</td>
 +
      <td>20</td>
 +
    </tr>
 +
    <tr>
 +
      <td>dNTP</td>
 +
      <td>1</td>
 +
      <td>8</td>
 +
    </tr>
 +
    <tr>
 +
      <td>VR</td>
 +
      <td>0.5</td>
 +
      <td>4</td>
 +
    </tr>
 +
    <tr>
 +
      <td>VF2</td>
 +
      <td>0.5</td>
 +
      <td>4</td>
 +
    </tr>
 +
    <tr>
 +
      <td>taq</td>
 +
      <td>0.2</td>
 +
      <td>1.6</td>
 +
    </tr>
 +
    <tr>
 +
      <td>dH2O</td>
 +
      <td>15.3</td>
 +
      <td>122.4</td>
 +
    </tr>
 +
  </tbody>
 +
</table>
 +
 +
<table class="table table-bordered table-striped">
 +
  <thead>
 +
    <th></th>
 +
    <th></th>
 +
    <th>DNA</th>
 +
    <th>H2O</th>
 +
    </tr>
 +
  </thead>
 +
  <tbody>
 +
    <tr>
 +
      <td>1</td>
 +
      <td>Control</td>
 +
      <td>0u</td>
 +
      <td>5u</td>
 +
    </tr>
 +
    <tr>
 +
      <td>2</td>
 +
      <td>J231013</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>3</td>
 +
      <td>GlmY-3A</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>4</td>
 +
      <td>2C</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>5</td>
 +
      <td>1C</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>6</td>
 +
      <td>1B</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>7</td>
 +
      <td>1A</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
  </tbody>
 +
</table>
 +
</div>
 +
</div>
 +
</div>
 +
 +
 +
<div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
 +
            <div class="panel panel-default">
 +
                <div class="panel-heading" role="tab" id="P5">
 +
                    <h4 class="panel-title">
 +
                    <a role="button" data-toggle="collapse" data-parent="#accordion" href="#P5-collapse" aria-expanded="false" aria-controls="P5-collapse">
 +
<div>
 +
                    <div class="col-md-11">10/10/2017 PCR check for J23102+GlmY</div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div>
 +
</div>
 +
                    </a>
 +
                    </h4>
 +
 +
                </div>
 +
                <div id="P5-collapse" class="panel-collapse collapse" role="tabpanel" aria-labelledby="P5">
 +
                    <div class="panel-body">
 +
<p>Master Mix</p>
 +
<table class="table table-bordered table-striped">
 +
  <tbody>
 +
    <tr>
 +
      <td>Buffer</td>
 +
      <td>2.5</td>
 +
      <td>20</td>
 +
    </tr>
 +
    <tr>
 +
      <td>dNTP</td>
 +
      <td>1</td>
 +
      <td>8</td>
 +
    </tr>
 +
    <tr>
 +
      <td>VR</td>
 +
      <td>0.5</td>
 +
      <td>4</td>
 +
    </tr>
 +
    <tr>
 +
      <td>VF2</td>
 +
      <td>0.5</td>
 +
      <td>4</td>
 +
    </tr>
 +
    <tr>
 +
      <td>Taq</td>
 +
      <td>0.2</td>
 +
      <td>1.6</td>
 +
    </tr>
 +
    <tr>
 +
      <td>dH2O</td>
 +
      <td>15.3</td>
 +
      <td>122.4</td>
 +
    </tr>
 +
  </tbody>
 +
</table>
 +
<table class="table table-bordered table-striped">
 +
  <thead>
 +
    <th></th>
 +
    <th></th>
 +
    <th>DNA</th>
 +
    <th>H2O</th>
 +
    </tr>
 +
  </thead>
 +
  <tbody>
 +
    <tr>
 +
      <td>1</td>
 +
      <td>Control</td>
 +
      <td>0u</td>
 +
      <td>5u</td>
 +
    </tr>
 +
    <tr>
 +
      <td>2</td>
 +
      <td>J231013</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>3</td>
 +
      <td>GlmY-3A</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>4</td>
 +
      <td>2C</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>5</td>
 +
      <td>1C</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>6</td>
 +
      <td>1B</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>7</td>
 +
      <td>1A</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
  </tbody>
 +
</table>
 +
<img class="pic" src="https://static.igem.org/mediawiki/2017/3/39/IMG_0207.jpeg">
 +
</div>
 +
</div>
 +
</div>
 +
 +
 +
<div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
 +
            <div class="panel panel-default">
 +
                <div class="panel-heading" role="tab" id="P6">
 +
                    <h4 class="panel-title">
 +
                    <a role="button" data-toggle="collapse" data-parent="#accordion" href="#P6-collapse" aria-expanded="false" aria-controls="P6-collapse">
 +
<div>
 +
                    <div class="col-md-11">10/20/2017 PCR J23102+GlmY</div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div>
 +
</div>
 +
                    </a>
 +
                    </h4>
 +
 +
                </div>
 +
                <div id="P6-collapse" class="panel-collapse collapse" role="tabpanel" aria-labelledby="P6">
 +
                    <div class="panel-body">
 +
<p>Master Mix</p>
 +
<table class="table table-bordered table-striped">
 +
  <tbody>
 +
    <tr>
 +
      <td>Buffer</td>
 +
      <td>2.5</td>
 +
      <td>20</td>
 +
    </tr>
 +
    <tr>
 +
      <td>dNTP</td>
 +
      <td>1</td>
 +
      <td>8</td>
 +
    </tr>
 +
    <tr>
 +
      <td>VR</td>
 +
      <td>0.5</td>
 +
      <td>4</td>
 +
    </tr>
 +
    <tr>
 +
      <td>VF2</td>
 +
      <td>0.5</td>
 +
      <td>4</td>
 +
    </tr>
 +
    <tr>
 +
      <td>Taq</td>
 +
      <td>0.2</td>
 +
      <td>1.6</td>
 +
    </tr>
 +
    <tr>
 +
      <td>dH2O</td>
 +
      <td>15.3</td>
 +
      <td>122.4</td>
 +
    </tr>
 +
  </tbody>
 +
</table>
 +
<table class="table table-bordered table-striped">
 +
  <thead>
 +
    <th></th>
 +
    <th></th>
 +
    <th>DNA</th>
 +
    <th>H2O</th>
 +
    </tr>
 +
  </thead>
 +
  <tbody>
 +
    <tr>
 +
      <td>1</td>
 +
      <td>Control</td>
 +
      <td>0u</td>
 +
      <td>5u</td>
 +
    </tr>
 +
    <tr>
 +
      <td>2</td>
 +
      <td>J23102B pp</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>3</td>
 +
      <td>J23102 P NNP</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>4</td>
 +
      <td>GlmY-3A</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>5</td>
 +
      <td>102B+GlmY 1B</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>6</td>
 +
      <td>102B+GlmY 1C</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>7</td>
 +
      <td>102B_GlmY 1D</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
  </tbody>
 +
</table>
 +
<img class="pic" src="https://static.igem.org/mediawiki/2017/5/54/IMG_0674.jpeg">
 +
</div>
 +
</div>
 +
</div>
 +
 +
 +
<div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
 +
            <div class="panel panel-default">
 +
                <div class="panel-heading" role="tab" id="P7">
 +
                    <h4 class="panel-title">
 +
                    <a role="button" data-toggle="collapse" data-parent="#accordion" href="#P7-collapse" aria-expanded="false" aria-controls="P7-collapse">
 +
<div>
 +
                    <div class="col-md-11">10/23/2017 Genomic PCR</div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div>
 +
</div>
 +
                    </a>
 +
                    </h4>
 +
 +
                </div>
 +
                <div id="P7-collapse" class="panel-collapse collapse" role="tabpanel" aria-labelledby="P7">
 +
                    <div class="panel-body">
 +
<p>Master Mix</p>
 +
<table class="table table-bordered table-striped">
 +
  <tbody>
 +
    <tr>
 +
      <td>H2O</td>
 +
      <td>13.3</td>
 +
      <td>106.4</td>
 +
    </tr>
 +
    <tr>
 +
      <td>Buffer</td>
 +
      <td>2.5</td>
 +
      <td>20</td>
 +
    </tr>
 +
    <tr>
 +
      <td>dNTP</td>
 +
      <td>1</td>
 +
      <td>8</td>
 +
    </tr>
 +
    <tr>
 +
      <td>GDNA</td>
 +
      <td>3</td>
 +
      <td>24</td>
 +
    </tr>
 +
    <tr>
 +
      <td>Taq</td>
 +
      <td>0.2</td>
 +
      <td>1.6</td>
 +
    </tr>
 +
  </tbody>
 +
</table>
 +
<p>Gel:</p>
 +
<table class="table table-bordered table-striped">
 +
<thead>
 +
        <tr>
 +
            <th></th>
 +
            <th>dH2O</th>
 +
            <th>For</th>
 +
            <th>Rev</th>
 +
            <th></th>
 +
        </tr>
 +
    </thead>
 +
<tbody>
 +
    <tr>
 +
      <td>1</td>
 +
      <td>5</td>
 +
      <td>-</td>
 +
      <td>-</td>
 +
      <td></td>
 +
    </tr>
 +
    <tr>
 +
      <td>2</td>
 +
      <td>4</td>
 +
      <td>0.5</td>
 +
      <td>0.5</td>
 +
      <td>AceF</td>
 +
    </tr>
 +
    <tr>
 +
      <td>3</td>
 +
      <td>4</td>
 +
      <td>0.5</td>
 +
      <td>0.5</td>
 +
      <td>EutG</td>
 +
    </tr>
 +
    <tr>
 +
      <td>4</td>
 +
      <td>4</td>
 +
      <td>0.5</td>
 +
      <td>0.5</td>
 +
      <td>YiaY</td>
 +
    </tr>
 +
    <tr>
 +
      <td>5</td>
 +
      <td>4</td>
 +
      <td>0.5</td>
 +
      <td>0.5</td>
 +
      <td>AdhP</td>
 +
    </tr>
 +
    <tr>
 +
      <td>6</td>
 +
      <td>4</td>
 +
      <td>0.5</td>
 +
      <td>0.5</td>
 +
      <td>Lpd</td>
 +
    </tr>
 +
    <tr>
 +
      <td>7</td>
 +
      <td>4</td>
 +
      <td>0.5</td>
 +
      <td>0.5</td>
 +
      <td>AceE</td>
 +
    </tr>
 +
  </tbody>
 +
</table>
 +
</div>
 +
</div>
 +
</div>
 +
 +
</body>
 
</html>
 
</html>
 
{{Team:Gaston_Day_School/Footer}}
 
{{Team:Gaston_Day_School/Footer}}

Latest revision as of 02:45, 2 November 2017

Notebook

On this page, Gaston Day School iGEM team would like to share with you our notes during the experiments from 09/01/2017 to 10/23/2017. The team leader Heena Saqib records the data patiently.

Master Mix:

14.3 H2O 114.4
2.5 Buffer 20
1 MgCl2 8
1 dNTP 8

tube:

2ul H2O
-5ul F
-5ul R

Label:

0 Control
1 AceF
2 EutG BB
3 YiaY BB
4 AdhP BB
5 Lpd
6 AceE

Master Mix

gDNA 3 24
Buffer 2.5 20
dNTP 1 8
dH2O 13.3 106.4
Taq 0.2 1.6
dH2O For Rev
1 4 0.5 0.5 AceF
2 4 0.5 0.5 EutG
3 4 0.5 0.5 YiaY
4 4 0.5 0.5 AdnP
5 4 0.5 0.5 Lpd
6 4 0.5 0.5 AceE
7 5 - - -

Master Mix

gDNA 3 24
Buffer 2.5 20
dNTP 1 8
dH2O 12.3 98.4
Taq 0.2 1.6
MgCl2 1 8
dH2O For Rev
0 5 - -
1 4 0.5 0.5 ACE F
2 4 0.5 0.5 EutG
3 4 0.5 0.5 YiaY
4 4 0.5 0.5 AdhP
5 4 0.5 0.5 Lpd
6 4 0.5 0.5 AceE
1 - DNA(0) dH2O(5)
2 J23102 3 2
3 GlmY 3 2
4 A(1) 3 2
5/td> A(2) 3 2
6 B(2) 3 2
7 C(2) 3 2

Max:

Buffer 2.5 20
dNTP 1 8
VR 0.5 4
VF2 0.5 4
Taq 0.2 1.6
dH2O 15.3 122.4

Master Mix

Buffer 2.5 20
dNTP 1 8
VR 0.5 4
VF2 0.5 4
taq 0.2 1.6
dH2O 15.3 122.4
DNA H2O
1 Control 0u 5u
2 J231013 3 2
3 GlmY-3A 3 2
4 2C 3 2
5 1C 3 2
6 1B 3 2
7 1A 3 2

Master Mix

Buffer 2.5 20
dNTP 1 8
VR 0.5 4
VF2 0.5 4
Taq 0.2 1.6
dH2O 15.3 122.4
DNA H2O
1 Control 0u 5u
2 J231013 3 2
3 GlmY-3A 3 2
4 2C 3 2
5 1C 3 2
6 1B 3 2
7 1A 3 2

Master Mix

Buffer 2.5 20
dNTP 1 8
VR 0.5 4
VF2 0.5 4
Taq 0.2 1.6
dH2O 15.3 122.4
DNA H2O
1 Control 0u 5u
2 J23102B pp 3 2
3 J23102 P NNP 3 2
4 GlmY-3A 3 2
5 102B+GlmY 1B 3 2
6 102B+GlmY 1C 3 2
7 102B_GlmY 1D 3 2

Master Mix

H2O 13.3 106.4
Buffer 2.5 20
dNTP 1 8
GDNA 3 24
Taq 0.2 1.6

Gel:

dH2O For Rev
1 5 - -
2 4 0.5 0.5 AceF
3 4 0.5 0.5 EutG
4 4 0.5 0.5 YiaY
5 4 0.5 0.5 AdhP
6 4 0.5 0.5 Lpd
7 4 0.5 0.5 AceE

 

Email: iGEM@gastonday.org

Gaston Day School

2001 Gaston Day School Rd

Gastonia 28056