Difference between revisions of "Team:Gaston Day School/Notebook"
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<div id="P1-collapse" class="panel-collapse collapse" role="tabpanel" aria-labelledby="P1"> | <div id="P1-collapse" class="panel-collapse collapse" role="tabpanel" aria-labelledby="P1"> | ||
<div class="panel-body"> | <div class="panel-body"> | ||
| − | <p> | + | <p>Master Mix:</p> |
<table class="table table-bordered table-striped"> | <table class="table table-bordered table-striped"> | ||
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<tbody> | <tbody> | ||
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<tr> | <tr> | ||
<td>4</td> | <td>4</td> | ||
| − | <td> | + | <td>AdhP BB</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>5</td> | <td>5</td> | ||
| − | <td> | + | <td>Lpd</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<a role="button" data-toggle="collapse" data-parent="#accordion" href="#P2-collapse" aria-expanded="false" aria-controls="P2-collapse"> | <a role="button" data-toggle="collapse" data-parent="#accordion" href="#P2-collapse" aria-expanded="false" aria-controls="P2-collapse"> | ||
<div> | <div> | ||
| − | <div class="col-md-11">09/06/2017 | + | <div class="col-md-11">09/06/2017 Genomic PCR</div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div> |
</div> | </div> | ||
</a> | </a> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>dNTP</td> |
<td>1</td> | <td>1</td> | ||
<td>8</td> | <td>8</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>Taq</td> |
<td>0.2</td> | <td>0.2</td> | ||
<td>1.6</td> | <td>1.6</td> | ||
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<thead> | <thead> | ||
<tr> | <tr> | ||
| − | <th> | + | <th></th> |
<th>dH2O</th> | <th>dH2O</th> | ||
<th>For</th> | <th>For</th> | ||
<th>Rev</th> | <th>Rev</th> | ||
| − | <th> | + | <th></th> |
</tr> | </tr> | ||
</thead> | </thead> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>Taq</td> |
<td>0.2</td> | <td>0.2</td> | ||
<td>1.6</td> | <td>1.6</td> | ||
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<a role="button" data-toggle="collapse" data-parent="#accordion" href="#P4-collapse" aria-expanded="false" aria-controls="P4-collapse"> | <a role="button" data-toggle="collapse" data-parent="#accordion" href="#P4-collapse" aria-expanded="false" aria-controls="P4-collapse"> | ||
<div> | <div> | ||
| − | <div class="col-md-11">09/18/2017 PCR check of J23102 | + | <div class="col-md-11">09/18/2017 PCR check of J23102+GlmY</div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div> |
</div> | </div> | ||
</a> | </a> | ||
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</tbody> | </tbody> | ||
</table> | </table> | ||
| − | <p>Max</p> | + | <p>Max:</p> |
<table class="table table-bordered table-striped"> | <table class="table table-bordered table-striped"> | ||
<tbody> | <tbody> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>Taq</td> |
<td>0.2</td> | <td>0.2</td> | ||
<td>1.6</td> | <td>1.6</td> | ||
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<a role="button" data-toggle="collapse" data-parent="#accordion" href="#P5-collapse" aria-expanded="false" aria-controls="P5-collapse"> | <a role="button" data-toggle="collapse" data-parent="#accordion" href="#P5-collapse" aria-expanded="false" aria-controls="P5-collapse"> | ||
<div> | <div> | ||
| − | <div class="col-md-11">10/10/2017 | + | <div class="col-md-11">10/10/2017 PCR check for J23102+GlmY</div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div> |
</div> | </div> | ||
</a> | </a> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>Taq</td> |
<td>0.2</td> | <td>0.2</td> | ||
<td>1.6</td> | <td>1.6</td> | ||
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</tbody> | </tbody> | ||
</table> | </table> | ||
| + | <img class="pic" src="https://static.igem.org/mediawiki/2017/3/39/IMG_0207.jpeg"> | ||
</div> | </div> | ||
</div> | </div> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>Taq</td> |
<td>0.2</td> | <td>0.2</td> | ||
<td>1.6</td> | <td>1.6</td> | ||
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<a role="button" data-toggle="collapse" data-parent="#accordion" href="#P7-collapse" aria-expanded="false" aria-controls="P7-collapse"> | <a role="button" data-toggle="collapse" data-parent="#accordion" href="#P7-collapse" aria-expanded="false" aria-controls="P7-collapse"> | ||
<div> | <div> | ||
| − | <div class="col-md-11">10/23/2017 | + | <div class="col-md-11">10/23/2017 Genomic PCR</div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div> |
</div> | </div> | ||
</a> | </a> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td>Taq</td> |
<td>0.2</td> | <td>0.2</td> | ||
<td>1.6</td> | <td>1.6</td> | ||
Latest revision as of 02:45, 2 November 2017
Notebook
On this page, Gaston Day School iGEM team would like to share with you our notes during the experiments from 09/01/2017 to 10/23/2017. The team leader Heena Saqib records the data patiently.
Master Mix:
| 14.3 | H2O | 114.4 |
| 2.5 | Buffer | 20 |
| 1 | MgCl2 | 8 |
| 1 | dNTP | 8 |
tube:
| 2ul | H2O |
| -5ul | F |
| -5ul | R |
Label:
| 0 | Control |
| 1 | AceF |
| 2 | EutG BB |
| 3 | YiaY BB |
| 4 | AdhP BB |
| 5 | Lpd |
| 6 | AceE |
Master Mix
| gDNA | 3 | 24 |
| Buffer | 2.5 | 20 |
| dNTP | 1 | 8 |
| dH2O | 13.3 | 106.4 |
| Taq | 0.2 | 1.6 |
| dH2O | For | Rev | ||
|---|---|---|---|---|
| 1 | 4 | 0.5 | 0.5 | AceF |
| 2 | 4 | 0.5 | 0.5 | EutG |
| 3 | 4 | 0.5 | 0.5 | YiaY |
| 4 | 4 | 0.5 | 0.5 | AdnP |
| 5 | 4 | 0.5 | 0.5 | Lpd |
| 6 | 4 | 0.5 | 0.5 | AceE |
| 7 | 5 | - | - | - |
Master Mix
| gDNA | 3 | 24 |
| Buffer | 2.5 | 20 |
| dNTP | 1 | 8 |
| dH2O | 12.3 | 98.4 |
| Taq | 0.2 | 1.6 |
| MgCl2 | 1 | 8 |
| dH2O | For | Rev | ||
|---|---|---|---|---|
| 0 | 5 | - | - | |
| 1 | 4 | 0.5 | 0.5 | ACE F |
| 2 | 4 | 0.5 | 0.5 | EutG |
| 3 | 4 | 0.5 | 0.5 | YiaY |
| 4 | 4 | 0.5 | 0.5 | AdhP |
| 5 | 4 | 0.5 | 0.5 | Lpd |
| 6 | 4 | 0.5 | 0.5 | AceE |
| 1 | - | DNA(0) | dH2O(5) |
|---|---|---|---|
| 2 | J23102 | 3 | 2 |
| 3 | GlmY | 3 | 2 |
| 4 | A(1) | 3 | 2 |
| 5/td> | A(2) | 3 | 2 |
| 6 | B(2) | 3 | 2 |
| 7 | C(2) | 3 | 2 |
Max:
| Buffer | 2.5 | 20 |
| dNTP | 1 | 8 |
| VR | 0.5 | 4 |
| VF2 | 0.5 | 4 |
| Taq | 0.2 | 1.6 |
| dH2O | 15.3 | 122.4 |
Master Mix
| Buffer | 2.5 | 20 |
| dNTP | 1 | 8 |
| VR | 0.5 | 4 |
| VF2 | 0.5 | 4 |
| taq | 0.2 | 1.6 |
| dH2O | 15.3 | 122.4 |
| DNA | H2O | ||
|---|---|---|---|
| 1 | Control | 0u | 5u |
| 2 | J231013 | 3 | 2 |
| 3 | GlmY-3A | 3 | 2 |
| 4 | 2C | 3 | 2 |
| 5 | 1C | 3 | 2 |
| 6 | 1B | 3 | 2 |
| 7 | 1A | 3 | 2 |
Master Mix
| Buffer | 2.5 | 20 |
| dNTP | 1 | 8 |
| VR | 0.5 | 4 |
| VF2 | 0.5 | 4 |
| Taq | 0.2 | 1.6 |
| dH2O | 15.3 | 122.4 |
| DNA | H2O | ||
|---|---|---|---|
| 1 | Control | 0u | 5u |
| 2 | J231013 | 3 | 2 |
| 3 | GlmY-3A | 3 | 2 |
| 4 | 2C | 3 | 2 |
| 5 | 1C | 3 | 2 |
| 6 | 1B | 3 | 2 |
| 7 | 1A | 3 | 2 |
Master Mix
| Buffer | 2.5 | 20 |
| dNTP | 1 | 8 |
| VR | 0.5 | 4 |
| VF2 | 0.5 | 4 |
| Taq | 0.2 | 1.6 |
| dH2O | 15.3 | 122.4 |
| DNA | H2O | ||
|---|---|---|---|
| 1 | Control | 0u | 5u |
| 2 | J23102B pp | 3 | 2 |
| 3 | J23102 P NNP | 3 | 2 |
| 4 | GlmY-3A | 3 | 2 |
| 5 | 102B+GlmY 1B | 3 | 2 |
| 6 | 102B+GlmY 1C | 3 | 2 |
| 7 | 102B_GlmY 1D | 3 | 2 |
Master Mix
| H2O | 13.3 | 106.4 |
| Buffer | 2.5 | 20 |
| dNTP | 1 | 8 |
| GDNA | 3 | 24 |
| Taq | 0.2 | 1.6 |
Gel:
| dH2O | For | Rev | ||
|---|---|---|---|---|
| 1 | 5 | - | - | |
| 2 | 4 | 0.5 | 0.5 | AceF |
| 3 | 4 | 0.5 | 0.5 | EutG |
| 4 | 4 | 0.5 | 0.5 | YiaY |
| 5 | 4 | 0.5 | 0.5 | AdhP |
| 6 | 4 | 0.5 | 0.5 | Lpd |
| 7 | 4 | 0.5 | 0.5 | AceE |
