Difference between revisions of "Team:MSU-Michigan/Design"
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{{MSU-Michigan}} | {{MSU-Michigan}} | ||
{{MSU-Michigan/W3}} | {{MSU-Michigan/W3}} | ||
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<html> | <html> | ||
| + | <script src="https://code.jquery.com/jquery-3.2.1.min.js"> //allow your page to use jQuery | ||
| + | </script> | ||
| + | <script> | ||
| + | $(function() { | ||
| + | $('.myDIV').hover( //when you hover over the figure | ||
| + | function() { | ||
| + | $(this).append($("<img id='char' src='https://static.igem.org/mediawiki/2017/0/03/Shewanella_demonstration.png'/>").hide().fadeIn()); //show the character | ||
| + | }, function() { | ||
| + | $('#char').remove(); //get rid of it after moving the cursor away from the figure | ||
| + | }); | ||
| + | }); | ||
| + | </script> | ||
| + | <style> | ||
| + | #char { | ||
| + | height:300px; | ||
| + | width:425px; | ||
| + | } | ||
| + | </style> | ||
| + | <!-- | ||
<style> | <style> | ||
* { | * { | ||
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/* Middle column */ | /* Middle column */ | ||
| − | + | .column.middle { | |
| + | width: 60%; | ||
} | } | ||
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} | } | ||
#char { | #char { | ||
| − | height: | + | height:200px; |
| − | width: | + | width:325px; |
} | } | ||
</style> | </style> | ||
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<div class="column side"></div> | <div class="column side"></div> | ||
<div class="column middle"> | <div class="column middle"> | ||
| − | + | --> | |
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<div class="w3-cell-row"> | <div class="w3-cell-row"> | ||
| − | <div class="myDIV"><p style="text-align:center"> | + | <div class="w3-col w3-container w3-light-green" style="width:17.5%"></div> |
| + | <div class="w3-col w3-container w3-pale-green" style="width:60%"> | ||
| + | <h1>Design</h1> | ||
| + | |||
| + | <!-- | ||
| + | <div class="myDIV"> | ||
| + | --> | ||
| + | <p style="text-align:center"> | ||
<img style="-webkit-user-select: none;background-position: 0px 0px, 10px 10px;background-size: 20px 20px;background-image:linear-gradient(45deg, #eee 25%, transparent 25%, transparent 75%, #eee 75%, #eee 100%),linear-gradient(45deg, #eee 25%, white 25%, white 75%, #eee 75%, #eee 100%);cursor: zoom-in;" src="https://static.igem.org/mediawiki/2017/0/05/MSU-Michigan_bioreactorassembly.png" width="800" height="403"> | <img style="-webkit-user-select: none;background-position: 0px 0px, 10px 10px;background-size: 20px 20px;background-image:linear-gradient(45deg, #eee 25%, transparent 25%, transparent 75%, #eee 75%, #eee 100%),linear-gradient(45deg, #eee 25%, white 25%, white 75%, #eee 75%, #eee 100%);cursor: zoom-in;" src="https://static.igem.org/mediawiki/2017/0/05/MSU-Michigan_bioreactorassembly.png" width="800" height="403"> | ||
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</p> | </p> | ||
<body> | <body> | ||
<p> Process for simple bioreactor assembly used to measure current produced by <i>Shewanella Oneidensis</i> strains when connected to a potentiostat </p> | <p> Process for simple bioreactor assembly used to measure current produced by <i>Shewanella Oneidensis</i> strains when connected to a potentiostat </p> | ||
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<p style="text-align:center"> | <p style="text-align:center"> | ||
| + | </body> | ||
<div class="myDIV"> | <div class="myDIV"> | ||
<p style="text-align:center"> | <p style="text-align:center"> | ||
| − | <iframe width="600" height="302" src="https:// | + | <iframe width="600" height="302" src="https://static.igem.org/mediawiki/2017/0/08/T--MSU-Michigan--bioreactorbiofilm.mp4" frameborder="0" allowfullscreen></iframe> |
</div> | </div> | ||
</p> | </p> | ||
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</p> | </p> | ||
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<h2>Bioreactors</h2> | <h2>Bioreactors</h2> | ||
<div class="w3-cell-row"> | <div class="w3-cell-row"> | ||
| − | <div class="w3-col w3-container" style="width: | + | <div class="w3-col w3-container" style="width:17.5%"></div> |
| − | <div class="w3-col w3-container" style="width: | + | <div class="w3-col w3-container" style="width:60%"> |
| − | <img src="https://static.igem.org/mediawiki/2017/0/08/T--MSU-Michigan--bioreactordiagram.png"> | + | <img src="https://static.igem.org/mediawiki/2017/0/08/T--MSU-Michigan--bioreactordiagram.png" style="width:100%"> |
</div> | </div> | ||
</div> | </div> | ||
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<div class="w3-cell-row"> | <div class="w3-cell-row"> | ||
| − | <div class="w3-col w3-container" style="width: | + | <div class="w3-col w3-container" style="width:17.5%"></div> |
| − | <div class="w3-col w3-container" style="width: | + | <div class="w3-col w3-container" style="width:60%"> |
<img src="https://static.igem.org/mediawiki/2017/7/7b/T--MSU-Michigan--pRL814mtrB.png" style="width:100%"> | <img src="https://static.igem.org/mediawiki/2017/7/7b/T--MSU-Michigan--pRL814mtrB.png" style="width:100%"> | ||
</div> | </div> | ||
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</html> | </html> | ||
| + | {{MSU-Michigan-Footer}} | ||
Latest revision as of 02:56, 2 November 2017
Design
Process for simple bioreactor assembly used to measure current produced by Shewanella Oneidensis strains when connected to a potentiostat
Bioreactors
Single chambered bioreactors were designed to be economical and functional. Reactors are run on magnetic stir plates and connected to a lab grade potentiostat.
Plasmid
To produce an electric current from Shewanella oneidensis MR-1 in response to chemicals in a sample, the mtrB gene was first removed from the genome (ΔmtrB) then re-introduced to the bacteria in a plasmid vector under an inducible promoter. The first promoter chosen was the well-studied IPTG inducible T7A1 promoter. Next, nine promoter sequences shown to respond to different effector molecules were identified and amplified from the S. oneidensis MR-1 genome with the addition of the restriction enzyme cut sites for AatII and NdeI on either end. Double digestion of both the vector and promoters using AatII and NdeI removed the T7A1 promoter and produced compatible sticky ends in the new promoter for insertion via ligation.
The designed constructs were individually transformed into E. coli WM3064, sequenced for confirmation, then conjugated into S. oneidensis MR-1 ∆mtrB. The S. oneidensis were then tested for induction through quantification of GFP fluorescence (marker protein) and current production using a spectrophotometer and bioreactor, respectively.
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