Difference between revisions of "Team:SDSZ-China/Safety"

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    <title>Project</title>
  
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<h1> Safety </h1>
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        #content {
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<p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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        h3 {
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<h5>Safe Project Design</h5>
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            font-size: 25 -px;
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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        #bg {
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            position: fixed;
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            z-index: -1;
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            top: 0;
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            left: 0;
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    </style>
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    <link href='http://fonts.googleapis.com/css?family=Lato' rel='stylesheet' type='text/css'>
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    <!-- 最新版本的 Bootstrap 核心 CSS 文件 -->
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    <link rel="stylesheet" href="https://cdn.bootcss.com/bootstrap/3.3.7/css/bootstrap.min.css"
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          integrity="sha384-BVYiiSIFeK1dGmJRAkycuHAHRg32OmUcww7on3RYdg4Va+PmSTsz/K68vbdEjh4u" crossorigin="anonymous">
  
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<ul>
 
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<li>Choosing a non-pathogenic chassis</li>
 
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<li>Choosing parts that will not harm humans / animals / plants</li>
 
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
 
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<li>Including an "induced lethality" or "kill-switch" device</li>
 
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</ul>
 
  
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</div>
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    <script>localStorage.clear();</script>
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    <!-- 最新的 Bootstrap 核心 JavaScript 文件 -->
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    <script src="https://cdn.bootcss.com/bootstrap/3.3.7/js/bootstrap.min.js"
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            integrity="sha384-Tc5IQib027qvyjSMfHjOMaLkfuWVxZxUPnCJA7l2mCWNIpG9mGCD8wGNIcPD7Txa"
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            crossorigin="anonymous"></script>
  
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<div class="column half_size">
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    <script type="text/javascript" async
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<h5>Safe Lab Work</h5>
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            src="https://cdn.mathjax.org/mathjax/latest/MathJax.js?config=TeX-MML-AM_CHTML"></script>
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    <script type="text/x-mathjax-config">
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        MathJax.Hub.Config({tex2jax: {inlineMath: [['$','$'], ['\\(','\\)']]}});
  
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
  
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</div>
 
  
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<div class="column half_size">
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<h5>Safe Shipment</h5>
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    </script>
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    <script type="text/javascript">
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        $(document).ready(function () {
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            $("#category_navbar a").on('click', function (event) {
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                // Make sure this.hash has a value before overriding default behavior
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                if (this.hash !== "") {
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                    // Prevent default anchor click behavior
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                    event.preventDefault();
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                    // Store hash0
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                    var hash = this.hash;
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                    // Using jQuery's animate() method to add smooth page scroll
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                    // The optional number (800) specifies the number of milliseconds it takes to scroll to the specified area
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                    $('html, body').animate({
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                        scrollTop: $(hash).offset().top
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                    }, 800, function () {
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                        // Add hash (#) to URL when done scrolling (default click behavior)
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                        window.location.hash = hash;
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                    });
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                }  // End if
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            });
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    </script>
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</head>
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</head>
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<body data-spy="scroll" data-target="#category_navbar">
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<div class="container" style='z-index:10;position:relative;'>
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    <div class="row card" id='bodycontainer' class='row'>
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        <nav class="navbar navbar-default navbar-static-top">
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            <!-- Brand and toggle get grouped for better mobile display -->
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            <div class="navbar-header">
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                <button type="button" class="navbar-toggle collapsed" data-toggle="collapse"
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                        data-target="#bs-example-navbar-collapse-1" aria-expanded="false">
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                    <span class="sr-only">Toggle navigation</span>
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                    <span class="icon-bar"></span>
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                    <span class="icon-bar"></span>
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                    <span class="icon-bar"></span>
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                </button>
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                <a class="navbar-brand" href="#"><img
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                        src="https://static.igem.org/mediawiki/2017/8/8c/%E5%91%80%E5%95%8A%E5%93%88.jpeg" width="100"></a>
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            </div>
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            <!-- Collect the nav links, forms, and other content for toggling -->
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            <div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1">
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                <ul class="nav navbar-nav">
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                    <li><a href="/Team:SDSZ-China">Home</a></li>
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                    <li><a href="/Team:SDSZ-China/Project">Project</a></li>
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                    <li><a href="/Team:SDSZ-China/Experiment">Experiment</a></li>
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                    <li><a href="/Team:SDSZ-China/Parts">Parts</a></li>
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                    <li><a href="/Team:SDSZ-China/Human_Practices">Human Practices</a></li>
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                    <li><a href="/Team:SDSZ-China/Safety">Safety</a></li>
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                    <li><a href="/Team:SDSZ-China/Hardware"> Hardware </a></li>
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                </ul>
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                <ul class="nav navbar-nav navbar-right">
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                    <li><a href="/Team:SDSZ-China/Team">Team and attributions</a></li>
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                    <li><a href="#">By SDSZ</a></li>
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                </ul>
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            </div><!-- /.navbar-collapse -->
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        </nav>
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        <div class="col-sm-2" style='padding-left:5% padding-right:10%'>
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            <div id="category_navbar">
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                <ul class="nav nav-list" data-spy="affix" data-offset-top="160"
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                    style='-webkit-transform: translateZ(0);width:160px;margin-left:0'>
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                    <li><a href="#Safety">Safety</a></li>
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                    <ul>
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                        <li><a href="#Bacteria">Bacteria we handled</a></li>
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                        <li><a href="#Chemicals">Chemicals we handled</a></li>
 +
                        <li><a href="#Lab">Lab environment</a></li>
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                        <li><a href="#Protocols">Safety protocols in lab</a></li>
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                    </ul>
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                </ul>
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        </div>
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        <div class="col-sm-10" style="padding-right:5%">
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            <h1 id="Safety">Safety</h1>
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            <h2 id="Bacteria">Bacteria we handled</h2>
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            <p>E.coli BL21(DE3) and DH5alpha, both are BSL1 organisms.</p>
 +
            <p></p>
 +
            <h2 id="Chemicals">Chemicals we handled</h2>
 +
            <p>We were exposed to mostly nonhazardous chemicals like agarose, LB and SOB agar, and their raw materials
 +
                like NaCl, KCl. We also handle the neurotoxic chemical polyacrylamide to tun the polyacrylamide gel
 +
                electrophoresis, and we did that under a chemical fume hood, with rubber gloves and lab coats on.</p>
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            <p></p>
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            <h2 id="Lab">Lab environment </h2>
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            <div class="row">
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                <div class="col-sm-6">
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                    <p>There are open bench, clean bench, and a chemical fume hood in our lab. We are well equipped with
 +
                        instruments for molecular biology experiments, such as Quantitative Real-time PCR System, Fluorescence
 +
                        microscope, Refrigerated Centrifuge, Metal Bath, Air Heating Incubator, etc. And the Lab for
 +
                        Microbiology Experiment was totally 40 square meters, with Biohazard Safety Equipment, Horizontal
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                        Laminar Flow Cabinet and other necessary instruments. It can be used for carrying out microbiology
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                        experiment with bio-safety level of P2.</p>
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                </div>
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                <figure class="col-sm-6">
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                    <img src="https://static.igem.org/mediawiki/2017/1/12/SDSZ-China_lab_picture.jpeg">
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                </figure>
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            </div>
 +
            <p></p>
 +
            <h2 id="Protocols">Safety protocols in lab</h2>
 +
            <p>In our high school, the lab guidelines focus on both instructing teachers to properly teach and students
 +
                to protect their own safety. It demands teachers should emphasize the lab safety rules and teach
 +
                students to proceed according to instructions; it clearly states that labs should remain ventilated when
 +
                harmful volatile chemicals are used during experiment, that fire sources in lab and preparation rooms
 +
                should be kept away from combustible materials, and that the injured should be immediately treated.</p>
 +
 
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            <br>
 +
            <br>
 +
            <br>
 +
        </div>
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    </div>
  
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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    <div class="backgroundpic">
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        <img src="https://static.igem.org/mediawiki/2017/e/e3/%E8%83%8C%E6%99%AF2jpeg.jpeg" width="100%"
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    </div>
 
</div>
 
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</body>
  
 
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Latest revision as of 02:58, 2 November 2017


Project

Safety

Bacteria we handled

E.coli BL21(DE3) and DH5alpha, both are BSL1 organisms.

Chemicals we handled

We were exposed to mostly nonhazardous chemicals like agarose, LB and SOB agar, and their raw materials like NaCl, KCl. We also handle the neurotoxic chemical polyacrylamide to tun the polyacrylamide gel electrophoresis, and we did that under a chemical fume hood, with rubber gloves and lab coats on.

Lab environment

There are open bench, clean bench, and a chemical fume hood in our lab. We are well equipped with instruments for molecular biology experiments, such as Quantitative Real-time PCR System, Fluorescence microscope, Refrigerated Centrifuge, Metal Bath, Air Heating Incubator, etc. And the Lab for Microbiology Experiment was totally 40 square meters, with Biohazard Safety Equipment, Horizontal Laminar Flow Cabinet and other necessary instruments. It can be used for carrying out microbiology experiment with bio-safety level of P2.

Safety protocols in lab

In our high school, the lab guidelines focus on both instructing teachers to properly teach and students to protect their own safety. It demands teachers should emphasize the lab safety rules and teach students to proceed according to instructions; it clearly states that labs should remain ventilated when harmful volatile chemicals are used during experiment, that fire sources in lab and preparation rooms should be kept away from combustible materials, and that the injured should be immediately treated.