Difference between revisions of "Team:UCopenhagen/Improve"
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<h2 class="section-heading"> Improved biobrick </h2> | <h2 class="section-heading"> Improved biobrick </h2> | ||
<p class="lead"> | <p class="lead"> | ||
| − | We have improved the function of a previous Biobrick: Original biobrick; http://parts.igem.org/Part:BBa_K864100. Our improved biobrick: http://parts.igem.org/Part:BBa_K2455002 | + | We have improved the function of a previous Biobrick: Original biobrick; <a href="http://parts.igem.org/Part:BBa_K864100">Super Yellow Fluorescent Protein 2</a>. Our improved biobrick: <a href="http://parts.igem.org/Part:BBa_K2455002">CPP-SYFP2</a>. |
<br><br> | <br><br> | ||
| − | + | CPP-SYFP2 (BBa_K2455002) is an improved version of the Super Yellow Fluorescent Protein 2 (BBa_K864100) carrying an N-terminal nona-arginine tag, and a C-terminal hexa-histidine tag. The R9-tag is a Cell-Penetrating Peptide giving it's associated protein the ability to pass through plasma membranes. | |
| − | and a C-terminal hexa-histidine tag. The R9-tag is a Cell-Penetrating Peptide giving it's associated protein the ability to pass through plasma membranes. | + | |
<br><br> | <br><br> | ||
| − | We made the improvement by inserting BBa_K864100 into the USER casette of our third biobrick, | + | We made the improvement by inserting BBa_K864100 into the USER casette of our third biobrick, <a href="http://parts.igem.org/Part:BBa_K2455003">the Cell-Penetrating USER Cassette</a>. |
<br> | <br> | ||
This biobrick contains an AsiSI/Nb.BSMI USER Cassette with a N-terminal nona-arginine (R9) Cell-penetrating peptide. | This biobrick contains an AsiSI/Nb.BSMI USER Cassette with a N-terminal nona-arginine (R9) Cell-penetrating peptide. | ||
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For our study we demonstrated that proteins inserted into the cassette can be purified using the histidine-tag, | For our study we demonstrated that proteins inserted into the cassette can be purified using the histidine-tag, | ||
and subsequently used to stain E. coli and Pseudomonas putida cells, presumably through internalization caused by the R9 peptide. | and subsequently used to stain E. coli and Pseudomonas putida cells, presumably through internalization caused by the R9 peptide. | ||
| + | <br><br> | ||
| − | + | See the <a href="2017.igem.org/Team:UCopenhagen/Results">results page</a> on this wiki, or go to the respective parts-pages for more information. | |
</p> | </p> | ||
Latest revision as of 03:03, 2 November 2017
Improved biobrick
We have improved the function of a previous Biobrick: Original biobrick; Super Yellow Fluorescent Protein 2. Our improved biobrick: CPP-SYFP2.
CPP-SYFP2 (BBa_K2455002) is an improved version of the Super Yellow Fluorescent Protein 2 (BBa_K864100) carrying an N-terminal nona-arginine tag, and a C-terminal hexa-histidine tag. The R9-tag is a Cell-Penetrating Peptide giving it's associated protein the ability to pass through plasma membranes.
We made the improvement by inserting BBa_K864100 into the USER casette of our third biobrick, the Cell-Penetrating USER Cassette.
This biobrick contains an AsiSI/Nb.BSMI USER Cassette with a N-terminal nona-arginine (R9) Cell-penetrating peptide.
The nona-arginine (R9) gives any cassette inserted proteins the ability to pass through plasma membranes.
In addition, the USER Cassette carries a N-terminal hexa-histidine tag which allows for easy purification.
For our study we demonstrated that proteins inserted into the cassette can be purified using the histidine-tag,
and subsequently used to stain E. coli and Pseudomonas putida cells, presumably through internalization caused by the R9 peptide.
See the results page on this wiki, or go to the respective parts-pages for more information.
