Difference between revisions of "Team:UCopenhagen/Improve"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Improve</h1>
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<p>For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Regisrty. Please include a link to your improved part on this page.</p>
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<h3></h3>
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                        <h1> I M P R O V E</h1>
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<h3>Gold Medal Criterion #2</h3>
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<p><b>Standard Tracks:</b> Improve the function of an existing BioBrick Part. The original part must NOT be from your 2017 part number range. If you change the original part sequence, you must submit a new part. In addition, both the new and original part pages must reference each other. This working part must be different from the part documented in bronze #4 and silver #1.
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                    <h2 class="section-heading"> Improved biobrick </h2>
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We have improved the function of a previous Biobrick: Original biobrick; <a href="http://parts.igem.org/Part:BBa_K864100">Super Yellow Fluorescent Protein 2</a>. Our improved biobrick: <a href="http://parts.igem.org/Part:BBa_K2455002">CPP-SYFP2</a>.
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<br><br>
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CPP-SYFP2 (BBa_K2455002) is an improved version of the Super Yellow Fluorescent Protein 2 (BBa_K864100) carrying an N-terminal nona-arginine tag, and a C-terminal hexa-histidine tag. The R9-tag is a Cell-Penetrating Peptide giving it's associated protein the ability to pass through plasma membranes.
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<br><br>
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We made the improvement by inserting BBa_K864100 into the USER casette of our third biobrick, <a href="http://parts.igem.org/Part:BBa_K2455003">the Cell-Penetrating USER Cassette</a>.
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<br>
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This biobrick contains an AsiSI/Nb.BSMI USER Cassette with a N-terminal nona-arginine (R9) Cell-penetrating peptide.
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The nona-arginine (R9) gives any cassette inserted proteins the ability to pass through plasma membranes.
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In addition, the USER Cassette carries a N-terminal hexa-histidine tag which allows for easy purification.
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<br>
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For our study we demonstrated that proteins inserted into the cassette can be purified using the histidine-tag,
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and subsequently used to stain E. coli and Pseudomonas putida cells, presumably through internalization caused by the R9 peptide.
 
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<b>Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
 
  
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See the <a href="2017.igem.org/Team:UCopenhagen/Results">results page</a> on this wiki, or go to the respective parts-pages for more information.
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</p>
  
 
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                    <h2>Find Incell here:</h2>
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<br>
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                            <a href="https://twitter.com/iGEM_Copenhagen" target="_blank" class="btn btn-default btn-lg"><i class="fa fa-twitter fa-fw"></i> <span class="network-name">Twitter</span></a>
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                            <a href="https://www.facebook.com/iGEMCopenhagen" target="_blank" class="btn btn-default btn-lg"><i class="fa fa-facebook fa-fw"></i> <span class="network-name">Facebook</span></a>
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                        </li>
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                            <a href="https://www.instagram.com/igemku2017" target="_blank" class="btn btn-default btn-lg"><i class="fa fa-instagram fa-fw"></i> <span class="network-name">Instagram</span></a>
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                <a class="page-scroll" href="#">Top</a>
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                        <a class="page-scroll" href="https://2017.igem.org/Team:UCopenhagen">Previous</a>
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                        <a class="page-scroll" href= "https://2017.igem.org/Team:UCopenhagen/Interdependency">Next</a>
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Latest revision as of 03:03, 2 November 2017

I M P R O V E

Improved biobrick

We have improved the function of a previous Biobrick: Original biobrick; Super Yellow Fluorescent Protein 2. Our improved biobrick: CPP-SYFP2.

CPP-SYFP2 (BBa_K2455002) is an improved version of the Super Yellow Fluorescent Protein 2 (BBa_K864100) carrying an N-terminal nona-arginine tag, and a C-terminal hexa-histidine tag. The R9-tag is a Cell-Penetrating Peptide giving it's associated protein the ability to pass through plasma membranes.

We made the improvement by inserting BBa_K864100 into the USER casette of our third biobrick, the Cell-Penetrating USER Cassette.
This biobrick contains an AsiSI/Nb.BSMI USER Cassette with a N-terminal nona-arginine (R9) Cell-penetrating peptide. The nona-arginine (R9) gives any cassette inserted proteins the ability to pass through plasma membranes. In addition, the USER Cassette carries a N-terminal hexa-histidine tag which allows for easy purification.
For our study we demonstrated that proteins inserted into the cassette can be purified using the histidine-tag, and subsequently used to stain E. coli and Pseudomonas putida cells, presumably through internalization caused by the R9 peptide.

See the results page on this wiki, or go to the respective parts-pages for more information.

Find Incell here: