Difference between revisions of "Team:UCopenhagen/Improve"

 
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<!-- Header -->
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<h3></h3>
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                        <h1> I M P R O V E</h1> 
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                    <h2 class="section-heading"> Improved biobrick </h2>
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We have improved the function of a previous Biobrick: Original biobrick; <a href="http://parts.igem.org/Part:BBa_K864100">Super Yellow Fluorescent Protein 2</a>. Our improved biobrick: <a href="http://parts.igem.org/Part:BBa_K2455002">CPP-SYFP2</a>.
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<br><br>
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CPP-SYFP2 (BBa_K2455002) is an improved version of the Super Yellow Fluorescent Protein 2 (BBa_K864100) carrying an N-terminal nona-arginine tag, and a C-terminal hexa-histidine tag. The R9-tag is a Cell-Penetrating Peptide giving it's associated protein the ability to pass through plasma membranes.
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<br><br>
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We made the improvement by inserting BBa_K864100 into the USER casette of our third biobrick, <a href="http://parts.igem.org/Part:BBa_K2455003">the Cell-Penetrating USER Cassette</a>.
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<br>
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This biobrick contains an AsiSI/Nb.BSMI USER Cassette with a N-terminal nona-arginine (R9) Cell-penetrating peptide.
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The nona-arginine (R9) gives any cassette inserted proteins the ability to pass through plasma membranes.
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In addition, the USER Cassette carries a N-terminal hexa-histidine tag which allows for easy purification.
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<br>
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For our study we demonstrated that proteins inserted into the cassette can be purified using the histidine-tag,
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and subsequently used to stain E. coli and Pseudomonas putida cells, presumably through internalization caused by the R9 peptide.
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<br><br>
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See the <a href="2017.igem.org/Team:UCopenhagen/Results">results page</a> on this wiki, or go to the respective parts-pages for more information.
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</p>
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        <div class="container">
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          <div class="row">
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                <div class="col-lg-5">
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                    <h2>Find Incell here:</h2>
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                </div>
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                <div class="col-lg-7">
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<br>
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                    <ul class="list-inline banner-social-buttons">
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                        <li>
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                            <a href="https://twitter.com/iGEM_Copenhagen" target="_blank" class="btn btn-default btn-lg"><i class="fa fa-twitter fa-fw"></i> <span class="network-name">Twitter</span></a>
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                        </li>
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                        <li>
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                            <a href="https://www.facebook.com/iGEMCopenhagen" target="_blank" class="btn btn-default btn-lg"><i class="fa fa-facebook fa-fw"></i> <span class="network-name">Facebook</span></a>
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                        </li>
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                        <li>
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                            <a href="https://www.instagram.com/igemku2017" target="_blank" class="btn btn-default btn-lg"><i class="fa fa-instagram fa-fw"></i> <span class="network-name">Instagram</span></a>
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                        </li>
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                    </ul>
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    <!-- Interactive bar -->
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        <div id="page-top" data-spy="scroll" data-target=".navbar-fixed-top">
 
     <!-- One-Page Scroll NavBar -->
 
     <!-- One-Page Scroll NavBar -->
 
     <nav class="navbar scrollnav-custom navbar-fixed-bottom" role="navigation" id="scrollnav">
 
     <nav class="navbar scrollnav-custom navbar-fixed-bottom" role="navigation" id="scrollnav">
 
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             <div class="navbar-header">
 
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                 <a class="navbar-brand" target="_blank"  href="https://www.facebook.com/iGEMCopenhagen">
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                 <a class="page-scroll" href="#">Top</a>
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                    <img src="https://static.igem.org/mediawiki/2017/f/fa/T--BOKU-Vienna--fb.png" alt="Facebook" >
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                <a class="navbar-brand" target="_blank"  href="https://www.twitter.com/igem_copenhagen">
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                    <img src="https://static.igem.org/mediawiki/2017/1/15/T--BOKU-Vienna--twitter.png" alt="Twitter" >
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                         <a class="page-scroll" href="#">Top</a>
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                         <a class="page-scroll" href="https://2017.igem.org/Team:UCopenhagen">Previous</a>
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                    </li>
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                    <li>
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                        <a class="page-scroll" href= "https://2017.igem.org/Team:UCopenhagen/Interdependency">Next</a>
 
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                        <h1 class="brand-heading">Interlab</h1>
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Latest revision as of 03:03, 2 November 2017

I M P R O V E

Improved biobrick

We have improved the function of a previous Biobrick: Original biobrick; Super Yellow Fluorescent Protein 2. Our improved biobrick: CPP-SYFP2.

CPP-SYFP2 (BBa_K2455002) is an improved version of the Super Yellow Fluorescent Protein 2 (BBa_K864100) carrying an N-terminal nona-arginine tag, and a C-terminal hexa-histidine tag. The R9-tag is a Cell-Penetrating Peptide giving it's associated protein the ability to pass through plasma membranes.

We made the improvement by inserting BBa_K864100 into the USER casette of our third biobrick, the Cell-Penetrating USER Cassette.
This biobrick contains an AsiSI/Nb.BSMI USER Cassette with a N-terminal nona-arginine (R9) Cell-penetrating peptide. The nona-arginine (R9) gives any cassette inserted proteins the ability to pass through plasma membranes. In addition, the USER Cassette carries a N-terminal hexa-histidine tag which allows for easy purification.
For our study we demonstrated that proteins inserted into the cassette can be purified using the histidine-tag, and subsequently used to stain E. coli and Pseudomonas putida cells, presumably through internalization caused by the R9 peptide.

See the results page on this wiki, or go to the respective parts-pages for more information.

Find Incell here: