Difference between revisions of "Team:IISc-Bangalore/Safety"

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{{IISc-Bangalore}}
 
{{IISc-Bangalore}}
 
<html>
 
<html>
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    <ol id="inPageNav">
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<li><a href="#lab">Lab Safety<img src="https://static.igem.org/mediawiki/2017/6/68/T--IISc-Bangalore--navbar_bullet.png" /></a></li>
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<li><a href="#gas-vesicles">Gas vesicles<img src="https://static.igem.org/mediawiki/2017/6/68/T--IISc-Bangalore--navbar_bullet.png" /></a></li>
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        <li><a href="#anabaena">Anabaena<img src="https://static.igem.org/mediawiki/2017/6/68/T--IISc-Bangalore--navbar_bullet.png" /></a></li>
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<li><a href="#strains">Strains<img src="https://static.igem.org/mediawiki/2017/6/68/T--IISc-Bangalore--navbar_bullet.png" /></a></li>
  
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    </ol>
  
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<div class="column full_size">
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<div id="contentMain">
  
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<h1> Safety </h1>
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<img src="https://static.igem.org/mediawiki/2017/c/c1/T--IISc-Bangalore--Header--safety.svg" id="headerImg" />
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<p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h1 id="lab">Lab Safety</h1>
  
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</div>
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<p>These photos depict relevant safety features of our lab.</p>
  
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<figure>
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<img src="https://static.igem.org/mediawiki/2017/6/67/T--IISc-Bangalore--lab-entry-rules.jpeg" width="70%">
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<figurecaption>Lab entry rules are clearly posted outside the lab: no external footwear allowed!</figurecaption>
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</figure>
  
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<div class="column full_size">
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<figure>
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<h5>Safe Project Design</h5>
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<img src="https://static.igem.org/mediawiki/2017/7/78/T--IISc-Bangalore--biosafety-cabinet.jpeg" width="80%">
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<figurecaption>
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A typical biosafety cabinet in our lab
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</figurecaption>
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</figure>
  
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<figure>
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<img src="https://static.igem.org/mediawiki/2017/f/f8/T--IISc-Bangalore--fume-hood.jpeg" width="50%">
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<figurecaption>
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Our fume hood, used for handling potentially hazardous chemicals
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</figurecaption>
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</figure>
  
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<ul>
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<figure>
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<li>Choosing a non-pathogenic chassis</li>
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<img src="https://static.igem.org/mediawiki/2017/7/7a/T--IISc-Bangalore--waste-disposal.jpeg" width="70%">
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<figurecaption>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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Waste disposal facility and hand wash station
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<li>Including an "induced lethality" or "kill-switch" device</li>
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</figurecaption>
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</ul>
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</figure>
  
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</div>
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<h1 id="gas-vesicles">Gas Vesicles</h1>
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<p>We are using purified gas vesicles from <i>Halobacterium salinarum</i> NRC-1 (a model archaeon) to demonstrate aggregation, but will not be working with the live organism. These gas vesicles are entirely non-immunogenic as demonstrated by <i>in vivo</i> studies in mammalian systems — they are non-toxic and harmless to humans.</p>
  
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<div class="column half_size">
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<h1 id="anabaena">Anabaena flos-aquae</h1>
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<p>We planned to culture Anabaena flos-aquae (UTEX 2557) and extract gas vesicles from it to test our methods of inducing clustering. This strain of Anabaena was chosen as it has lost the ability to produce anatoxin-a and is axenic (no bacterial contamination).</p>
  
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<figure>
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<img src="https://static.igem.org/mediawiki/2017/6/6f/T--IISc-Bangalore--anabaena.png">
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<br>
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<figurecaption>
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This strain of Anabaena was chosen due to these Curator's notes
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</figurecaption>
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</figure>
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<h1 id="strains">Strains</h1>
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<p>We used common lab strains of E. coli for our chassis. DH5α was used for cloning due to its high transformation efficiency and its recA and endA mutations. BL21 (DE3) was used to overexpress our fusion protein under the T7 expression system as it contains the gene encoding the T7 RNA polymerase.</p>
 
</div>
 
</div>
  
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<div class="column half_size">
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<script>
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<h5>Safe Shipment</h5>
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window.slide = new SlideNav({
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  changeHash: true   
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});
  
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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var height = $('#headerImg').height();
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</div>
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Latest revision as of 03:15, 2 November 2017

  1. Lab Safety
  2. Gas vesicles
  3. Anabaena
  4. Strains

Lab Safety

These photos depict relevant safety features of our lab.

Lab entry rules are clearly posted outside the lab: no external footwear allowed!
A typical biosafety cabinet in our lab
Our fume hood, used for handling potentially hazardous chemicals
Waste disposal facility and hand wash station

Gas Vesicles

We are using purified gas vesicles from Halobacterium salinarum NRC-1 (a model archaeon) to demonstrate aggregation, but will not be working with the live organism. These gas vesicles are entirely non-immunogenic as demonstrated by in vivo studies in mammalian systems — they are non-toxic and harmless to humans.

Anabaena flos-aquae

We planned to culture Anabaena flos-aquae (UTEX 2557) and extract gas vesicles from it to test our methods of inducing clustering. This strain of Anabaena was chosen as it has lost the ability to produce anatoxin-a and is axenic (no bacterial contamination).


This strain of Anabaena was chosen due to these Curator's notes

Strains

We used common lab strains of E. coli for our chassis. DH5α was used for cloning due to its high transformation efficiency and its recA and endA mutations. BL21 (DE3) was used to overexpress our fusion protein under the T7 expression system as it contains the gene encoding the T7 RNA polymerase.