Difference between revisions of "Team:IISc-Bangalore/Safety"

 
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<h1 id="lab">Lab Safety</h1>
 
<h1 id="lab">Lab Safety</h1>
  
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These photos depict relevant safety features of our lab.
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<p>These photos depict relevant safety features of our lab.</p>
  
 
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<img src="https://static.igem.org/mediawiki/2017/6/67/T--IISc-Bangalore--lab-entry-rules.jpeg" width="70%">
 
<img src="https://static.igem.org/mediawiki/2017/6/67/T--IISc-Bangalore--lab-entry-rules.jpeg" width="70%">
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<figurecaption>Lab entry rules are clearly posted outside the lab: no external footwear allowed!</figurecaption>
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<img src="https://static.igem.org/mediawiki/2017/7/7d/T--IISc-Bangalore--personal-protective-equipment.jpeg" width="70%">
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<img src="https://static.igem.org/mediawiki/2017/7/78/T--IISc-Bangalore--biosafety-cabinet.jpeg" width="80%">
 
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A typical biosafety cabinet in our lab
 
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Our fume hood, used for handling potentially hazardous chemicals
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<img src="https://static.igem.org/mediawiki/2017/7/7a/T--IISc-Bangalore--waste-disposal.jpeg" width="70%">
 
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Waste disposal facility and hand wash station
 
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<h1 id="gas-vesicles">Gas Vesicles</h1>
 
<h1 id="gas-vesicles">Gas Vesicles</h1>
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<p>We are also using purified gas vesicles from <i>Halobacterium salinarum</i> NRC-1 (a model archaeon) for the same purpose, but will not be working with the live organism.</p>
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<p>We are using purified gas vesicles from <i>Halobacterium salinarum</i> NRC-1 (a model archaeon) to demonstrate aggregation, but will not be working with the live organism. These gas vesicles are entirely non-immunogenic as demonstrated by <i>in vivo</i> studies in mammalian systems — they are non-toxic and harmless to humans.</p>
  
 
<h1 id="anabaena">Anabaena flos-aquae</h1>
 
<h1 id="anabaena">Anabaena flos-aquae</h1>
  
 
<p>We planned to culture Anabaena flos-aquae (UTEX 2557) and extract gas vesicles from it to test our methods of inducing clustering. This strain of Anabaena was chosen as it has lost the ability to produce anatoxin-a and is axenic (no bacterial contamination).</p>
 
<p>We planned to culture Anabaena flos-aquae (UTEX 2557) and extract gas vesicles from it to test our methods of inducing clustering. This strain of Anabaena was chosen as it has lost the ability to produce anatoxin-a and is axenic (no bacterial contamination).</p>
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<img src="https://static.igem.org/mediawiki/2017/6/6f/T--IISc-Bangalore--anabaena.png">
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This strain of Anabaena was chosen due to these Curator's notes
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<h1 id="strains">Strains</h1>
 
<h1 id="strains">Strains</h1>
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<p>We will be using common lab strains of E. coli for our chassis. DH5alpha will be used for cloning purposes due to its high transformation efficiency and its recA and endA mutations. BL21 (DE3) will be used to over-express our fusion proteins under the T7 expression system as it contains the gene encoding the T7 RNA polymerase.</p>
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<p>We used common lab strains of E. coli for our chassis. DH5α was used for cloning due to its high transformation efficiency and its recA and endA mutations. BL21 (DE3) was used to overexpress our fusion protein under the T7 expression system as it contains the gene encoding the T7 RNA polymerase.</p>
 
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Latest revision as of 03:15, 2 November 2017

  1. Lab Safety
  2. Gas vesicles
  3. Anabaena
  4. Strains

Lab Safety

These photos depict relevant safety features of our lab.

Lab entry rules are clearly posted outside the lab: no external footwear allowed!
A typical biosafety cabinet in our lab
Our fume hood, used for handling potentially hazardous chemicals
Waste disposal facility and hand wash station

Gas Vesicles

We are using purified gas vesicles from Halobacterium salinarum NRC-1 (a model archaeon) to demonstrate aggregation, but will not be working with the live organism. These gas vesicles are entirely non-immunogenic as demonstrated by in vivo studies in mammalian systems — they are non-toxic and harmless to humans.

Anabaena flos-aquae

We planned to culture Anabaena flos-aquae (UTEX 2557) and extract gas vesicles from it to test our methods of inducing clustering. This strain of Anabaena was chosen as it has lost the ability to produce anatoxin-a and is axenic (no bacterial contamination).


This strain of Anabaena was chosen due to these Curator's notes

Strains

We used common lab strains of E. coli for our chassis. DH5α was used for cloning due to its high transformation efficiency and its recA and endA mutations. BL21 (DE3) was used to overexpress our fusion protein under the T7 expression system as it contains the gene encoding the T7 RNA polymerase.