Difference between revisions of "Team:SDSZ-China"

 
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<h1> Project Description </h1>
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Bear bile, as one of the most famous animal drugs in Traditional Chinese Medicine (TCM), has been recorded in ancient Chinese medicine book as a significant method to treat hepatic and biliary disorders including cancers. Also, its marked pharmacological effects on fever fighting, detoxification and pain reduction have been confirmed through modern investigations. However, the increasing demand of bear bile has caused bears to be in an endangered state: bear poaching and illegal animal trade have greatly dwindled the number of the wild Asiatic black bear. Apart from that, bear bile farming industry has established in Asia to extract bile through “milking” from the bears, which is basically operated through surgically implanting a permanent catheter in the animal’s gall bladder then obtain the drips. It is unquestionable that the bear bile farming process will lead to both physically and psychologically damage in bears.
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To find substitutes or alternative of bear bile farming, our team will be working on biological synthesis of the main effective component of this important medicine, UDCA. And this biological method will be more efficient and also cheaper than the original chemical approach, which is used in the current chemical UDCA synthesis industry.
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We found that it is possible to convert the main component of goose bile, CDCA, into UDCA, by using two enzymes to catalyst the reaction. The reaction will proceed in two steps. First, CDCA is oxidized by the enzyme 7α-HSDH, coupled by the regeneration of NAD+ with the enzyme LDH, and pyruvate. Similarly, in the second reduction step, the reaction is catalyzed by the enzyme 7β-HSDH, with the reaction that generates NADPH, which takes glucose as reactant and GDH as enzyme.
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Our mission now is to get the four enzymes (7α-HSDH, 7β-HSDH, GDH, and LDH) by expressing their gene in E.coli. After expression, 7α-HSDH and 7β-HSDH will be able to bind to a chitin column because a gene encoding a chitin binding domain is inserted, so no father purification process will be needed. Then we will examine the activity of the enzymes by measuring the rate of NAD+, NADPH production. At last a machine will be made, including the reaction efficiency measuring system, and the enzyme addition controlling system.
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<h5>Before you start: </h5>
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<p> Please read the following pages:</p>
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<li>  <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
 
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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                    <h1 class="text-danger">SDSZ - China</h1>
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                    <h4 class="text-default">Automatic Synthesize of UDCA(effective ingredient of bear bile) using immobilized enzymes.</h4>
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Latest revision as of 03:24, 2 November 2017


Project



SDSZ - China

Automatic Synthesize of UDCA(effective ingredient of bear bile) using immobilized enzymes.