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| − | <div class="container" style='z-index:10;position:relative;'>
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| − | <a href="https://2016.igem.org/Team:TAS_Taipei/Background"><h4 class="dropdown-toggle disabled"
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| − | data-toggle="dropdown"><b>PROJECT</b>
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| − | </h4></a>
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| − | <div class='subcategories_container'>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Background">Background</a></h5>
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| − | </div>
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| − | <div>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Achievements">Achievements</a></h5>
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| − | </div>
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| − | <div>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Collaborations">Collaborations</a>
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| − | </h5>
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| − | </div>
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| − | <div>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Parts">Parts</a></h5>
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| − | </div>
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| − | </div>
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| − | <div style='height:140px;'>
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| − | <img src="https://static.igem.org/mediawiki/2016/1/11/T--TAS_Taipei--TAS_Icon_Project.png">
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| − | <h4><b>Cataracts</b> - the leading cause of blindness. Find out how we can non-invasively
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| − | <b>treat</b> and <b>prevent</b> cataract formation.</b></h4>
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| − | </div>
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| − | </li>
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| − | <li class="dropdown">
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| − | <a href="https://2016.igem.org/Team:TAS_Taipei/Experimental_Summary"><h4
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| − | class='dropdown-toggle disabled' data-toggle="dropdown"><b>EXPERIMENTAL</b></h4></a>
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| − | <div class="dropdown-menu">
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| − | <div class='subcategories_container'>
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| − | <div>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Experimental_Summary#lensmodel">Lens
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| − | Cataract Model</a></h5>
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| − | </div>
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| − | <div>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Experimental_Summary#construct">Prevention
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| − | and Treatment Constructs </a></h5>
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| − | </div>
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| − | <div>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Experimental_Summary#prototype">Delivery
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| − | Prototype</a></h5>
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| − | </div>
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| − | <div>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Notebook">Notebook</a></h5>
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| − | </div>
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| − | </div>
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| − | <div style='height:140px;'>
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| − | <img src="https://static.igem.org/mediawiki/2016/b/b0/T--TAS_Taipei--TAS_Icon_Experiments.png">
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| − | <h4>We build <b>constructs</b> to make our great ideas become reality. Follow along our
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| − | discovery of exciting science!</h4>
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| − | </div>
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| − | </div>
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| − | </li>
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| − | <a href="https://2016.igem.org/Team:TAS_Taipei/Model"><h4 class='dropdown-toggle disabled'
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| − | data-toggle="dropdown"><b>MODEL</b></h4>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Model#GSRFunc">GSR Function</a></h5>
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| − | </div>
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| − | <div>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Model#prototype">Prototype
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| − | Delivery</a></h5>
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| − | </div>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Model#treatment">CH25H Treatment</a>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Model#software">Calculator</a></h5>
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| − | <div style='height:140px;'>
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| − | <img src="https://static.igem.org/mediawiki/2016/c/ca/T--TAS_Taipei--TAS_Icon_Model.png">
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| − | <h4><b>Computational Biology</b> provides us models that we cannot easily test
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| − | experimentally. Click to find out the results of our modeling, and the math behind it!
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| − | </h4>
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| − | </div>
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| − | data-toggle="dropdown"><b>HUMAN
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| − | PRACTICE</b></h4></a>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Human_Practices#research">Research</a>
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| − | </h5>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Human_Practices#outreach">Outreach</a>
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| − | </div>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Human_Practices#impact">Impact</a>
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| − | <div style='height:140px;'>
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| − | <img src="https://static.igem.org/mediawiki/2016/b/b9/T--TAS_Taipei--TAS_Icon_HP.png">
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| − | <h4>We don't just grow cool bacteria. We make a <b>difference</b>. Find out how we tackled
| + | |
| − | <b>social aspects</b> of this project.</h4>
| + | |
| − | </div>
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| − | </div>
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| − | </li>
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| − | <li class="dropdown">
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| − | <a href="https://2016.igem.org/Team:TAS_Taipei/Safety"><h4 class='dropdown-toggle disabled'
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| − | data-toggle="dropdown"><b>SAFETY</b></h4>
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| − | </a>
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| − | <div class="dropdown-menu">
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| − | <div style='height:100px;'>
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| − | <div class='subcategories_container'>
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| − | <div>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Safety#overview">Overview</a></h5>
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| − | </div>
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| − | <div>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Safety#biosafety">Biosafety</a>
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| − | </h5>
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| − | </div>
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| − | <div>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Safety#riskreduction">Risk
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| − | Reduction Methods</a></h5>
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| − | </div>
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| − | <div>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Safety#furtherquestion">Further
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| − | Questions</a></h5>
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| − | </div>
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| − | </div>
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| − | <img src="https://static.igem.org/mediawiki/2016/7/7e/T--TAS_Taipei--TAS_Icon_Safety.png">
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| − | <h4><b>Safety first.</b> Especially in a lab. Here's how we maintained and investigated the
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| − | integrity and security of our work environment.</h4>
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| − | </div>
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| − | </div>
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| − | </li>
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| − | <li class="dropdown">
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| − | <a href="https://2016.igem.org/Team:TAS_Taipei/Team"><h4 class='dropdown-toggle disabled'
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| − | data-toggle="dropdown"><b>TEAM</b></h4></a>
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| − | <div class="dropdown-menu">
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| − | <div class='subcategories_container'>
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| − | <div>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Team#members">Members</a></h5>
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| − | </div>
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| − | <div>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Team#about">About</a></h5>
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| − | </div>
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| − | <div>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Attributions">Attributions</a></h5>
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| − | </div>
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| − | <div>
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| − | <h5><a href="https://2016.igem.org/Team:TAS_Taipei/Wiki_Standard_Pages">Wiki Standard
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| − | Pages</a></h5>
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| − | </div>
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| − | </div>
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| − | <div style='height:140px;'>
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| − | <img src="https://static.igem.org/mediawiki/2016/c/c6/T--TAS_Taipei--TAS_Icon_Team.png">
| + | |
| − | <h4>Every iGEM project is the accumulation of an entire year's hard work by a group of
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| − | cheerful students. <b>Meet the team!</b></h4>
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| − | </div>
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| − | <div class="col-sm-2">
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| − | <a href="https://2016.igem.org/Team:TAS_Taipei" style='text-decoration: none'><img src="https://static.igem.org/mediawiki/2016/c/c6/T--TAS_Taipei--TAS_iGEM_Logo.png
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| − | <h2 style="font-family:'Lato';letter-spacing:10px;color: white; font-size: 60px; margin-top: 0; margin-bottom: 0;">
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| − | <b>
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| − | C◎UNTERACTS</b></h2>
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| − | <a href="https://igem.org/HS"><img
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| − | <div id="category_navbar">
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| − | <ul class="nav nav-list" data-spy="affix" data-offset-top="160"
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| − | style='-webkit-transform: translateZ(0);width:160px;margin-left:0'>
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| − | <li><a href="#cataract">What are Cataracts?</a></li>
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| − | <li><a href="#solution">What is our Solution?</a></li>
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| − | <h1 id='overview'>Background</h1>
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| − | <p>Cataracts are the leading cause of blindness today, affecting 20 million people worldwide (World
| + | |
| − | Health Organization). Half of Americans above 80 years old are affected by cataracts (National
| + | |
| − | Eye Institute), and so are many animals! The National Eye Institute projects that in 30 years,
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| − | the number of cataract patients will increase to 50 million (National Eye Institute). </p>
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| − | </div>
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| − | <h2 id='cataract'>What are Cataracts?</h2>
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| − | <div class="row">
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| − | <div class="col-sm-6">
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| − | <p>
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| − | The lens is mostly made of proteins called crystallins. Crystallin proteins are normally
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| − | soluble, which keeps the lens clear and allows light entering the eye to focus. When
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| − | these proteins are damaged, they form insoluble clumps (Truscott, 2005). This causes the
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| − | clouding seen in cataractous lenses, which scatters light and in turn makes vision
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| − | blurry (Figure 1.1).
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| − | | + | |
| − | </p>
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| − | </div>
| + | |
| − | <figure class="col-sm-6">
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| − | <img src="https://static.igem.org/mediawiki/2016/f/ff/T--TAS_Taipei--Normal_vs_Cataract_Model_cropped.png">
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| − | <figcaption class='darkblue'><b>Figure 1.1. </b>Cataracts scatter light coming through the
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| − | lens, which blurs vision.
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| − | </figcaption>
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| − | </figure>
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| − | </div>
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| − | <br><br>
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| − | <div class="row">
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| − | <div class="col-sm-6">
| + | |
| − | <p>
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| − | Cataracts can be caused by many factors, including radiation and diabetes, but the
| + | |
| − | underlying cause is oxidative damage. Oxidative damage happens when unstable chemicals
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| − | containing oxygen react with DNA, lipids, or proteins, disrupting cellular functions
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| − | (Truscott, 2005). In the lens, crystallin proteins can be oxidized by hydrogen peroxide
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| − | (H₂O₂), which is a reactive molecule produced during aerobic respiration (Giorgio et
| + | |
| − | al., 2007). H₂O₂ reacts with protein residues and changes the shape of the protein. When
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| − | two cysteine residues on separate proteins are oxidized by H₂O₂, for example, they can
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| − | form a disulfide bond, which links these proteins together. The damaged proteins thus
| + | |
| − | aggregate and form clumps in the lens (Truscott, 2005) (Figure 1.2).
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| − | | + | |
| − | | + | |
| − | </p>
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| − | </div>
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| − | <figure class="col-sm-6">
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| − | <img src="https://static.igem.org/mediawiki/2016/9/92/T--TAS_Taipei--Oxidative_Damage.jpeg">
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| − | <figcaption class='darkblue'><b>Figure 1.2. </b>Oxidative damage by H₂O₂ can lead to
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| − | proteins misfolding, breaking apart, and clumping.
| + | |
| − | </figcaption>
| + | |
| − | </figure>
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| − | | + | |
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| − | </div>
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| − | <br><br>
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| − | <div class="row">
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| − | <p>
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| − | <b>In the eye, a natural antioxidant called glutathione (GSH) exists,</b> which can
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| − | convert H₂O₂ into water (Giblin, 2000). With age, however, GSH levels decrease, and
| + | |
| − | oxidative damage caused by H₂O₂ increases. When there is more H₂O₂ in the lens than GSH
| + | |
| − | can remove, crystallins become damaged (Figure 1.3). When GSH levels are low, H₂O₂
| + | |
| − | starts to oxidize crystallins and cause cataracts. As lens cells age, they move towards
| + | |
| − | the nucleus and their GSH levels fall (Cvekl & Ashery-Padan, 2014), which may explain
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| − | why the older cells in the lens nucleus are more prone to developing cataracts
| + | |
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| − | </p>
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| − | <br><br>
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| − | <img src="https://static.igem.org/mediawiki/2016/5/5d/T--TAS_Taipei--Antioxidant_Balance.jpeg">
| + | |
| − | <figcaption class='darkblue'><b>Figure 1.3.</b> Antioxidants protects proteins from
| + | |
| − | oxidative damage by H₂O₂ (left). When antioxidant levels are low, H₂O₂ damages
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| − | crystallins and cataract develops (right).
| + | |
| − | </figcaption>
| + | |
| − | </figure>
| + | |
| − | <div class="col-sm-1"></div>
| + | |
| − | <figure class="col-sm-4">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2016/6/6c/T--TAS_Taipei--New_vs_Old_Cells.jpeg">
| + | |
| − | <figcaption class='darkblue'><b>Figure 1.4.</b> Lens cells move towards the nucleus as they
| + | |
| − | mature. Older cells have less GSH and are more susceptible to oxidative damage by H₂O₂.
| + | |
| − | </figcaption>
| + | |
| − | </figure>
| + | |
| − | </div>
| + | |
| − | <br><br>
| + | |
| − | <div class="row">
| + | |
| − | <div class="col-sm-7">
| + | |
| − | <p>
| + | |
| − | The current standard treatment for cataracts is surgery, which replaces the cloudy lens
| + | |
| − | with a clear artificial lens. Surgery is effective, but like all surgeries, it is <b>invasive
| + | |
| − | and requires professional equipment and trained surgeons.</b> These requirements add to
| + | |
| − | the cost, which averages about $3,500 per eye in the US without insurance (Sigre, 2016),
| + | |
| − | and is the biggest obstacle to solving cataracts worldwide. Through literature research,
| + | |
| − | we found a molecule called 25-hydroxycholesterol (25HC) that can reverse protein
| + | |
| − | aggregation. We hope to use this as an alternative to surgery to treat cataracts.
| + | |
| − | | + | |
| − | </p>
| + | |
| − | </div>
| + | |
| − | <figure class="col-sm-5">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2016/e/e2/T--TAS_Taipei--Surgery_Cataract.jpeg">
| + | |
| − | <figcaption class='darkblue' style="font-color:red"><b>Figure 1.5.</b> Our goal is to
| + | |
| − | replace surgery with noninvasive eye drops that prevent and treat cataracts.
| + | |
| − | </figcaption>
| + | |
| − | </figure>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | </div> <!-- Container -->
| + | |
| − | </div> <!-- Container -->
| + | |
| − | | + | |
| − | | + | |
| − | <div class="row">
| + | |
| − | <div class="col-sm-12">
| + | |
| − | <h2 id='solution'>What is our Solution?</h2>
| + | |
| − | <div class="row">
| + | |
| − | <div class="col-sm-12">
| + | |
| − | <p>
| + | |
| − | Our goal is to develop noninvasive, easy-to-use, and affordable eyedrops to prevent and
| + | |
| − | treat cataracts (Figure 1.5).
| + | |
| − | </p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="row">
| + | |
| − | | + | |
| − | <div class="col-sm-12">
| + | |
| − | <h3 id="prevention">Prevention</h3>
| + | |
| − | <p>
| + | |
| − | When GSH is present, H₂O₂ can oxidize GSH instead of crystallin proteins. When GSH
| + | |
| − | becomes oxidized, a disulfide bond forms between two GSH molecules, which become
| + | |
| − | oxidized glutathione (GSSG). Through literature research, we found an enzyme that
| + | |
| − | recycles GSSG back into GSH. This enzyme is called glutathione reductase (GSR) (Ganea &
| + | |
| − | Harding, 2006). As seen in Figure 1.6, GSR (green) recycles GSSG back into GSH, so that
| + | |
| − | crystallin proteins remain protected. Even though GSR exists in the lens, its levels
| + | |
| − | decrease with age, which leads to the development of cataracts (Michael & Bron, 2011).
| + | |
| − | <b>Our goal is to independently produce and deliver GSR to the lens, so that cataract
| + | |
| − | formation is prevented. </b>
| + | |
| − | | + | |
| − | </p>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | </div>
| + | |
| − | <div class="row">
| + | |
| − | | + | |
| − | | + | |
| − | <div class="col-sm-4"><img
| + | |
| − | src="https://static.igem.org/mediawiki/2016/b/b0/T--TAS_Taipei--ClearAnimation1.gif"></div>
| + | |
| − | | + | |
| − | <div class="col-sm-4"><img
| + | |
| − | src="https://static.igem.org/mediawiki/2016/3/30/T--TAS_Taipei--BlurredAnimation1.gif"></div>
| + | |
| − | | + | |
| − | <div class="col-sm-4"><img
| + | |
| − | src="https://static.igem.org/mediawiki/2016/7/7f/T--TAS_Taipei--AnimatinoLegend.gif"></div>
| + | |
| − | | + | |
| − | <figure class="col-sm-12">
| + | |
| − | <figcaption class='darkblue'><b>Figure 1.6 </b>In a normal lens (left), GSR (enzyme, in
| + | |
| − | green) converts GSSG into GSH. H₂O₂ oxidizes GSH instead of crystallin proteins, so that
| + | |
| − | crystallin proteins remain protected, and the lens remains clear. In a cataractous lens
| + | |
| − | (right), GSR levels are low so GSH cannot be remade. Since GSH is not present to protect
| + | |
| − | proteins against H₂O₂, cataracts begin to develop.
| + | |
| − | </figcaption>
| + | |
| − | </figure>
| + | |
| − | | + | |
| − | | + | |
| − | </div>
| + | |
| − | <br><br>
| + | |
| − | | + | |
| − | <div class="row">
| + | |
| − | | + | |
| − | <div class="col-sm-8">
| + | |
| − | <h3 id="treatment">Treatment</h3>
| + | |
| − | <p>
| + | |
| − | We also found a molecule that can restore solubility of protein clumps and lens
| + | |
| − | transparency. It is called 25-hydroxycholesterol (25HC) (Makley et al., 2015). 25HC can
| + | |
| − | be produced from cholesterol by the enzyme cholesterol 25-hydroxylase (CH25H) (Figure
| + | |
| − | 1.7). Our goal is to independently produce and deliver CH25H to the lens, so that
| + | |
| − | cataracts can be treated.
| + | |
| − | | + | |
| − | </p>
| + | |
| − | </div>
| + | |
| − | <figure class="col-sm-4">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2016/7/7c/T--TAS_Taipei--CH25HRxnDescription.png">
| + | |
| − | <figcaption class='darkblue'><b>Figure 1.7. </b>CH25H (enzyme) converts cholesterol to 25HC,
| + | |
| − | which can reverse protein clumps.
| + | |
| − | </figcaption>
| + | |
| − | </figure>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | </div>
| + | |
| − | | + | |
| − | | + | |
| − | </div> <!-- Container -->
| + | |
| − | <br><br>
| + | |
| − | <div class="row">
| + | |
| − | <div class="col-sm-12">
| + | |
| − | <h3>Citations</h3>
| + | |
| − | <p>Cvekl, A., & Ashery-Padan, R. (2014). The cellular and molecular mechanisms of vertebrate lens
| + | |
| − | development. Development, 141(23), 4432-4447.
| + | |
| − | </p>
| + | |
| − | <br>
| + | |
| − | <p>Ganea, E. & Harding, J. J. (2006). Glutathione-related enzymes and the eye. Curr Eye Res., 31(1),
| + | |
| − | 1–11
| + | |
| − | </p> <br>
| + | |
| − | <p>Giblin, F. J. (2000). Glutathione: a vital lens antioxidant. Journal of Ocular Pharmacology and
| + | |
| − | Therapeutics, 16(2), 121-135.
| + | |
| − | </p> <br>
| + | |
| − | <p>Giorgio, M., Trinei, M., Migliaccio, E., & Pelicci, P. (2007). Nature Reviews Molecular Cell
| + | |
| − | Biology, 8(9), 722-8.
| + | |
| − | </p> <br>
| + | |
| − | <p>Makley, L. N., McMenimen, K. A., DeVree, B. T., Goldman, J. W., McGlasson, B. N., Rajagopal, P.,
| + | |
| − | Dunyak, B.M., McQuade, T.J., Thompson, A.D., Sunahara, R., Klevit, R.E., Andley, U.P., and
| + | |
| − | Gestwicki, J.E. (2015). Pharmacological chaperone for α-crystallin partially restores
| + | |
| − | transparency in cataract models. Science, 350(6261), 674-677.
| + | |
| − | </p> <br>
| + | |
| − | <p>Michael, R., & Bron, A. J. (2011). The ageing lens and cataract: a model of normal and
| + | |
| − | pathological ageing. Philosophical Transactions of the Royal Society of London B: Biological
| + | |
| − | Sciences, 366(1568), 1278-1292.
| + | |
| − | </p> <br>
| + | |
| − | <p>National Eye Institute | Cataracts. (n.d.). Retrieved October 04, 2016, from
| + | |
| − | https://nei.nih.gov/eyedata/cataract
| + | |
| − | </p> <br>
| + | |
| − | <p>Segre L (2016, Sept. 21). Cataract surgery cost. Retrieved from
| + | |
| − | http://www.allaboutvision.com/conditions/cataract-surgery-cost.htm
| + | |
| − | </p> <br>
| + | |
| − | <p>Truscott, RJ (2005). Age-related nuclear cataract-oxidation is the key. Exp Eye Res., 80(5):
| + | |
| − | 709-25.
| + | |
| − | </p> <br>
| + | |
| − | <p>World Health Organization | Priority eye diseases. (n.d.). Retrieved October 03, 2016, from
| + | |
| − | http://www.who.int/blindness/causes/priority/en/index1.html
| + | |
| − | </p> <br>
| + | |
| − | <p></p> <br> <br>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | | + | |
| − | </div>
| + | |
| − | | + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <br>
| + | |
| − | <br><br>
| + | |
| − | | + | |
| − | <canvas id="canvas-container" style="z-index:-1"></canvas>
| + | |
| − | <script type="text/javascript"
| + | |
| − | src='https://2016.igem.org/Template:TAS_Taipei/js/field?action=raw&ctype=text/javascript'></script>
| + | |
| − | | + | |
| − | </body>
| + | |
| − | | + | |
| − | | + | |
| − | <style type='text/css'>
| + | |
| − | #bg {
| + | |
| − | position: fixed;
| + | |
| − | top: 0;
| + | |
| − | left: 0;
| + | |
| − | }
| + | |
| − | | + | |
| − | .bgwidth {
| + | |
| − | width: 100%;
| + | |
| − | }
| + | |
| − | | + | |
| − | .bgheight {
| + | |
| − | height: 100%;
| + | |
| − | }
| + | |
| − | </style>
| + | |
| − | | + | |
| − | <div class="backgroundpic">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2016/e/e5/T--TAS_Taipei--Taipei_Background_1.0.jpg" style="z-index:0"
| + | |
| − | width="100%" height="100%" id="bg" alt="">
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <script>
| + | |
| − | var nowRadius = 0
| + | |
| − | $(function () {
| + | |
| − | if (screen.width > 1800)
| + | |
| − | $("#slideoutco").fadeOut(0);
| + | |
| − | | + | |
| − | $({blurRadius: 0}).animate({blurRadius: 10}, {
| + | |
| − | duration: 20000,
| + | |
| − | easing: 'swing', // or "linear"
| + | |
| − | // use jQuery UI or Easing plugin for more options
| + | |
| − | step: function () {
| + | |
| − | console.log(this.blurRadius);
| + | |
| − | if ($("#bluebutton").hasClass("isOn")) {
| + | |
| − | return;
| + | |
| − | }
| + | |
| − | ;
| + | |
| − | if ($('#redbutton').hasClass("isOn")) {
| + | |
| − | return;
| + | |
| − | }
| + | |
| − | $('.backgroundpic').css({
| + | |
| − | "-webkit-filter": "blur(" + this.blurRadius + "px)",
| + | |
| − | "filter": "blur(" + this.blurRadius + "px)"
| + | |
| − | | + | |
| − | });
| + | |
| − | nowRadius = this.blurRadius;
| + | |
| − | var LOCSnum = Math.round(nowRadius * 6 / 9);
| + | |
| − | var NineLOCSnum = Math.round(nowRadius * 6 / 9 * 10);
| + | |
| − | /**if (NineLOCSnum > 20) switchMessage("YELLOW","<b> Oh no! </b> Cataracts are forming! Click on the Eyedrop Tab and get <b>TREATMENT</b> eyedrops!", currentColor()=="GREEN");
| + | |
| − | if (NineLOCSnum == 55) $("#slideoutco").fadeOut(1000);
| + | |
| − | if (NineLOCSnum > 60) switchMessage("RED","Your cataracts are very severe. You need to get TREATMENT fast by clicking the Eyedrop Tab", currentColor()=="YELLOW");*/
| + | |
| − | if (LOCSnum > 6) {
| + | |
| − | LOCSnum = "6+";
| + | |
| − | $('#LOCS').text(LOCSnum + "");
| + | |
| − | }
| + | |
| − | else $('#LOCS').text(LOCSnum + "");
| + | |
| − | | + | |
| − | if (LOCSnum == 0) document.getElementById('bluebutton').click();
| + | |
| − | }
| + | |
| − | });
| + | |
| − | | + | |
| − | });
| + | |
| − | | + | |
| − | startBlur = function (speed) {
| + | |
| − | $({blurRadius: nowRadius}).animate({blurRadius: 10}, {
| + | |
| − | duration: speed,
| + | |
| − | easing: 'swing', // or "linear"
| + | |
| − | // use jQuery UI or Easing plugin for more options
| + | |
| − | step: function () {
| + | |
| − | console.log(this.blurRadius);
| + | |
| − | if ($("#bluebutton").hasClass("isOn")) {
| + | |
| − | return;
| + | |
| − | }
| + | |
| − | ;
| + | |
| − | if ($('#redbutton').hasClass("isOn")) {
| + | |
| − | return;
| + | |
| − | }
| + | |
| − | | + | |
| − | $('.backgroundpic').css({
| + | |
| − | "-webkit-filter": "blur(" + this.blurRadius + "px)",
| + | |
| − | "filter": "blur(" + this.blurRadius + "px)"
| + | |
| − | | + | |
| − | });
| + | |
| − | | + | |
| − | nowRadius = this.blurRadius;
| + | |
| − | var LOCSnum = Math.round(nowRadius * 6 / 9);
| + | |
| − | var NineLOCSnum = Math.round(nowRadius * 6 / 9 * 10);
| + | |
| − | /**if (NineLOCSnum == 15) $("#slideoutco").fadeOut(400);
| + | |
| − | if (NineLOCSnum > 20) switchMessage("RED","<b>Cataracts</b> are creeping back again! Click the PREVENTION eyedrop to add GSR into your eyes!", currentColor()=="BLUE");*/
| + | |
| − | if (LOCSnum > 6) {
| + | |
| − | LOCSnum = "6+";
| + | |
| − | $('#LOCS').text(LOCSnum + "");
| + | |
| − | }
| + | |
| − | else $('#LOCS').text(LOCSnum + " ");
| + | |
| − | | + | |
| − | | + | |
| − | }
| + | |
| − | });
| + | |
| − | };
| + | |
| − | | + | |
| − | stopBlur = function (speed) {
| + | |
| − | | + | |
| − | $({blurRadius: nowRadius}).animate({blurRadius: 0}, {
| + | |
| − | duration: speed,
| + | |
| − | easing: 'swing', // or "linear"
| + | |
| − | // use jQuery UI or Easing plugin for more options
| + | |
| − | step: function () {
| + | |
| − | console.log(this.blurRadius);
| + | |
| − | if ($("#redbutton").hasClass("isOn")) {
| + | |
| − | } else {
| + | |
| − | return;
| + | |
| − | }
| + | |
| − | ;
| + | |
| − | $('.backgroundpic').css({
| + | |
| − | "-webkit-filter": "blur(" + this.blurRadius + "px)",
| + | |
| − | "filter": "blur(" + this.blurRadius + "px)"
| + | |
| − | | + | |
| − | });
| + | |
| − | nowRadius = this.blurRadius;
| + | |
| − | var LOCSnum = Math.round(nowRadius * 6 / 9);
| + | |
| − | var NineLOCSnum = Math.round(nowRadius * 6 / 9 * 10);
| + | |
| − | | + | |
| − | /**if (NineLOCSnum < 68){ if (NineLOCSnum > 10) if(currentColor()=="RED" || currentColor() == "BLUE") $("#slideoutco").fadeOut(0); switchMessage("YELLOW","<b> Treating</b> your cataract with <b>25HC Eyedrops </b>", currentColor()=="RED");
| + | |
| − | }
| + | |
| − | if (NineLOCSnum == 9) $("#slideoutco").fadeOut(0);
| + | |
| − | | + | |
| − | | + | |
| − | if (NineLOCSnum < 5) {
| + | |
| − | if ($("#bluebutton").hasClass("isOn")){
| + | |
| − | | + | |
| − | switchMessage("GREEN","<b>Your eyes are <i>pernamently</i> crystal clear!</b> Treatment is not needed, so don't forget to turn it off!. Click the question mark to learn more.", currentColor()=="BLUE")
| + | |
| − | }
| + | |
| − | else switchMessage("BLUE","Your eyes are crystal clear! To avoid waste, please turn off the <b>TREATMENT</b> eyedrop.", currentColor()=="YELLOW");
| + | |
| − | }*/
| + | |
| − | if (LOCSnum > 6) LOCSnum = "6+";
| + | |
| − | else LOCSnum = LOCSnum + " ";
| + | |
| − | $('#LOCS').text(LOCSnum + "");
| + | |
| − | | + | |
| − | }
| + | |
| − | });
| + | |
| − | | + | |
| − | | + | |
| − | };
| + | |
| − | | + | |
| − | function chooseBlur() {
| + | |
| − | if ($("#redbutton").hasClass("isOn")) {
| + | |
| − | stopBlur(4500);
| + | |
| − | | + | |
| | } | | } |
| | | | |
| − | else { | + | body { |
| − | if ($("#bluebutton").hasClass("isOn")) { | + | font-family: Lato !important; |
| − | }
| + | |
| − | else {
| + | |
| − | startBlur(12000);
| + | |
| − | }
| + | |
| | } | | } |
| − | /**if (!$("#redbutton").hasClass("isOn"))
| |
| − | if ($("#bluebutton").hasClass("isOn"))
| |
| − | {
| |
| − | $("#slideoutco").fadeOut(0);
| |
| − | switchMessage("BLUE","Great! Furthur cataract formation is prevented with GSR Eyedrops. Now use <b>TREATMENT</b> eyedrops one last time.", currentColor()=="RED")
| |
| − | }*/
| |
| − | };
| |
| | | | |
| − | | + | h3 { |
| − | </script>
| + | font-size: 25 -px; |
| − | | + | |
| − | <script>
| + | |
| − | | + | |
| − | function switchToggleB() {
| + | |
| − | if ($("#bluebutton").hasClass("isOn")) { | + | |
| − | $("#bluebutton").removeClass("isOn"); | + | |
| | } | | } |
| − | else {
| |
| − | $("#bluebutton").addClass("isOn");
| |
| − | }
| |
| − | };
| |
| | | | |
| − | function switchToggleR() {
| + | #bg { |
| − | if ($("#redbutton").hasClass("isOn")) { | + | position: fixed; |
| − | $("#redbutton").removeClass("isOn"); | + | z-index: -1; |
| | + | top: 0; |
| | + | left: 0; |
| | } | | } |
| − | else {
| |
| − | $("#redbutton").addClass("isOn");
| |
| − | }
| |
| − | };
| |
| | | | |
| − | function currentColor() {
| + | #bodycontainer { |
| − | if ($("#slidecontenttext").hasClass("alert-danger")) | + | background: rgba(255, 255, 255, 0.5); |
| − | return "RED"; | + | |
| − | if ($("#slidecontenttext").hasClass("alert-warning"))
| + | |
| − | return "YELLOW";
| + | |
| − | if ($("#slidecontenttext").hasClass("alert-info"))
| + | |
| − | return "BLUE";
| + | |
| − | if ($("#slidecontenttext").hasClass("alert-success"))
| + | |
| − | return "GREEN";
| + | |
| − | else return "NONE"
| + | |
| − | };
| + | |
| − | | + | |
| − | function switchMessage(color, textInside, refresh) {
| + | |
| − | var entirebox = document.getElementById("slideoutco");
| + | |
| − | | + | |
| − | if (refresh) {
| + | |
| − | $(entirebox).fadeOut(100);
| + | |
| − | if ($("#slidecontenttext").hasClass("alert-danger"))
| + | |
| − | $("#slidecontenttext").removeClass("alert-danger");
| + | |
| − | if ($("#slidecontenttext").hasClass("alert-warning"))
| + | |
| − | $("#slidecontenttext").removeClass("alert-warning");
| + | |
| − | if ($("#slidecontenttext").hasClass("alert-info"))
| + | |
| − | $("#slidecontenttext").removeClass("alert-info");
| + | |
| − | if ($("#slidecontenttext").hasClass("alert-success"))
| + | |
| − | $("#slidecontenttext").removeClass("alert-success");
| + | |
| − | if (color == "RED")
| + | |
| − | $("#slidecontenttext").addClass("alert-danger");
| + | |
| − | if (color == "YELLOW")
| + | |
| − | $("#slidecontenttext").addClass("alert-warning");
| + | |
| − | if (color == "BLUE")
| + | |
| − | $("#slidecontenttext").addClass("alert-info");
| + | |
| − | if (color == "GREEN")
| + | |
| − | $("#slidecontenttext").addClass("alert-success");
| + | |
| − | | + | |
| − | | + | |
| − | $("#slidecontenttext").html(textInside);
| + | |
| − | $(entirebox).fadeIn(500);
| + | |
| | } | | } |
| | + | </style> |
| | + | <link href='http://fonts.googleapis.com/css?family=Lato' rel='stylesheet' type='text/css'> |
| | + | <!-- 最新版本的 Bootstrap 核心 CSS 文件 --> |
| | + | <link rel="stylesheet" href="https://cdn.bootcss.com/bootstrap/3.3.7/css/bootstrap.min.css" |
| | + | integrity="sha384-BVYiiSIFeK1dGmJRAkycuHAHRg32OmUcww7on3RYdg4Va+PmSTsz/K68vbdEjh4u" crossorigin="anonymous"> |
| | | | |
| − | };
| |
| | | | |
| | + | <script>localStorage.clear();</script> |
| | + | <!-- 最新的 Bootstrap 核心 JavaScript 文件 --> |
| | + | <script src="https://cdn.bootcss.com/bootstrap/3.3.7/js/bootstrap.min.js" |
| | + | integrity="sha384-Tc5IQib027qvyjSMfHjOMaLkfuWVxZxUPnCJA7l2mCWNIpG9mGCD8wGNIcPD7Txa" |
| | + | crossorigin="anonymous"></script> |
| | | | |
| − | </script> | + | <script type="text/javascript" async |
| | + | src="https://cdn.mathjax.org/mathjax/latest/MathJax.js?config=TeX-MML-AM_CHTML"></script> |
| | + | <script type="text/x-mathjax-config"> |
| | + | MathJax.Hub.Config({tex2jax: {inlineMath: [['$','$'], ['\\(','\\)']]}}); |
| | | | |
| | | | |
| − | <canvas id="canvas-container" style="z-index:-1" hidden></canvas>
| |
| − | <script type="text/javascript" src='https://2015.igem.org/Template:TAS_Taipei/js/field?action=raw&ctype=text/javascript'
| |
| − | hidden></script/>
| |
| | | | |
| | + | |
| | + | </script> |
| | | | |
| − | <script type="text/javascript"> | + | <script type="text/javascript"> |
| − | /* Toggle between adding and removing the "active" and "show" classes when the user clicks on one of the "Section" buttons. The "active" class is used to add a background color to the current button when its belonging panel is open. The "show" class is used to open the specific accordion panel */
| + | |
| − | var acc = document.getElementsByClassName("accordion");
| + | |
| − | var i;
| + | |
| | | | |
| − | for (i = 0; i < acc.length; i++) {
| + | $(document).ready(function () { |
| − | acc[i].onclick = function () {
| + | $("#category_navbar a").on('click', function (event) { |
| − | this.classList.toggle("active"); | + | // Make sure this.hash has a value before overriding default behavior |
| − | this.nextElementSibling.classList.toggle("show");
| + | if (this.hash !== "") { |
| − | }
| + | // Prevent default anchor click behavior |
| − | }
| + | event.preventDefault(); |
| − | </script>
| + | |
| | | | |
| − | <script type="text/javascript">
| + | // Store hash0 |
| − | $("#category_navbar a").on('click', function (event) {
| + | var hash = this.hash; |
| − | // Make sure this.hash has a value before overriding default behavior
| + | |
| − | if (this.hash !== "") {
| + | |
| − | // Prevent default anchor click behavior
| + | |
| − | event.preventDefault();
| + | |
| | | | |
| − | // Store hash0
| + | // Using jQuery's animate() method to add smooth page scroll |
| − | var hash = this.hash;
| + | // The optional number (800) specifies the number of milliseconds it takes to scroll to the specified area |
| | + | $('html, body').animate({ |
| | + | scrollTop: $(hash).offset().top |
| | + | }, 800, function () { |
| | | | |
| − | // Using jQuery's animate() method to add smooth page scroll
| + | // Add hash (#) to URL when done scrolling (default click behavior) |
| − | // The optional number (800) specifies the number of milliseconds it takes to scroll to the specified area
| + | window.location.hash = hash; |
| − | $('html, body').animate({
| + | }); |
| − | scrollTop: $(hash).offset().top
| + | } // End if |
| − | }, 800, function () {
| + | |
| − | | + | |
| − | // Add hash (#) to URL when done scrolling (default click behavior)
| + | |
| − | window.location.hash = hash;
| + | |
| | }); | | }); |
| − | } // End if | + | }); |
| − | });
| + | |
| | | | |
| | | | |
| − | </script> | + | </script> |
| | | | |
| − | <div id="slideout" display="none">
| + | </head> |
| − | <div id="slidecontent">
| + | </head> |
| − | <h3>Prevention</h3>
| + | |
| − | <h5>GSR Eyedrop</h5>
| + | |
| − | <label class="switch">
| + | |
| − | <input id="bluebutton" onClick="switchToggleB(); chooseBlur()" type="checkbox">
| + | |
| − | <div class="slider round bluecolorbutton"></div>
| + | |
| − | </label>
| + | |
| − | <br>
| + | |
| | | | |
| − | <h3>Treatment</h3>
| + | <body data-spy="scroll" data-target="#category_navbar"> |
| − | <h5>25HC Eyedrop</h5>
| + | |
| − | <label class="switch">
| + | |
| − | <input id="redbutton" onClick="switchToggleR(); chooseBlur()" type="checkbox">
| + | |
| − | <div class="slider round redcolorbutton"></div>
| + | |
| − | </label>
| + | |
| − | <h4><b> LOCS: <span id="LOCS">0  </span></b>     <a
| + | |
| − | href="https://2016.igem.org/Team:TAS_Taipei/Wiki_Standard_Pages#Animation">
| + | |
| − | <button type="button" class="btn btn-danger btn-md">?</button>
| + | |
| − | </a>
| + | |
| | | | |
| − | </div>
| + | <div class="container" style='z-index:10;position:relative;'> |
| − | <div id="clickme">
| + | |
| − | <h2 class="vertical-text" style="Lato" !important>
| + | |
| − | <br> Eyedrops
| + | |
| − | </h2>
| + | |
| − | <br><br><br>
| + | |
| | | | |
| − | </div>
| |
| − | </div>
| |
| | | | |
| − | <div id="slideoutco">
| + | <div class="row card" id='bodycontainer' class='row'> |
| − | <div id="slidecontentco"> | + | |
| − | <div id="slidecontenttext" class="alert alert-danger">
| + | |
| − | <p style="font-size:14px !important"><a href="#" class="close" data-dismiss="alert">×</a>
| + | |
| − | <strong>Zoom out to see animation.</strong>          <a
| + | |
| − | href="https://2016.igem.org/Team:TAS_Taipei/Wiki_Standard_Pages#Animation">
| + | |
| − | <button type="button" class="btn btn-danger btn-sm">?</button>
| + | |
| − | </a><span style="font-size:14px"><br>Your screen resolution is too low unless you zoom out </span></p>
| + | |
| | | | |
| − | </div> | + | <nav class="navbar navbar-default navbar-static-top"> |
| − | </div>
| + | <!-- Brand and toggle get grouped for better mobile display --> |
| − | </div> | + | <div class="navbar-header"> |
| | + | <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" |
| | + | data-target="#bs-example-navbar-collapse-1" aria-expanded="false"> |
| | + | <span class="sr-only">Toggle navigation</span> |
| | + | <span class="icon-bar"></span> |
| | + | <span class="icon-bar"></span> |
| | + | <span class="icon-bar"></span> |
| | + | </button> |
| | + | <a class="navbar-brand" href="#"><img |
| | + | src="https://static.igem.org/mediawiki/2017/8/8c/%E5%91%80%E5%95%8A%E5%93%88.jpeg" width="100"></a> |
| | + | </div> |
| | | | |
| − | <javascript> | + | <!-- Collect the nav links, forms, and other content for toggling --> |
| | + | <div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1"> |
| | + | <ul class="nav navbar-nav"> |
| | + | <li><a href="/Team:SDSZ-China">Home</a></li> |
| | + | <li><a href="/Team:SDSZ-China/Project">Project</a></li> |
| | + | <li><a href="/Team:SDSZ-China/Experiment">Experiment</a></li> |
| | + | <li><a href="/Team:SDSZ-China/Parts">Parts</a></li> |
| | + | <li><a href="/Team:SDSZ-China/Human_Practices">Human Practices</a></li> |
| | + | <li><a href="/Team:SDSZ-China/Safety">Safety</a></li> |
| | + | <li><a href="/Team:SDSZ-China/Hardware"> Hardware </a></li> |
| | | | |
| − | </javascript> | + | </ul> |
| | + | <ul class="nav navbar-nav navbar-right"> |
| | + | <li><a href="/Team:SDSZ-China/Team">Team and attributions</a></li> |
| | + | <li><a href="#">By SDSZ</a></li> |
| | + | </ul> |
| | + | </div><!-- /.navbar-collapse --> |
| | + | </nav> |
| | | | |
| − | <style type='text/css'>
| |
| | | | |
| − | #slideout {
| + | <div class="col-sm-12" style="padding-right:5%"> |
| − | background: #FFD700; | + | |
| − | position: fixed;
| + | |
| − | height: 300px;
| + | |
| − | width: 200px;
| + | |
| − | top: 30%;
| + | |
| − | right: -150px;
| + | |
| − | padding-left: 60px;
| + | |
| − | z-index: 30;
| + | |
| − | border-radius: 25px;
| + | |
| | | | |
| − | }
| + | <br> |
| | + | <br> |
| | | | |
| − | #slideoutco {
| + | <div class="text-center"> |
| − | position: fixed;
| + | <h1 class="text-danger">SDSZ - China</h1> |
| − | height: 200px;
| + | <h4 class="text-default">Automatic Synthesize of UDCA(effective ingredient of bear bile) using immobilized enzymes.</h4> |
| − | width: 300px;
| + | </div> |
| − | top: 78%;
| + | |
| − | right: 1%;
| + | |
| − | padding-left: 10px;
| + | |
| − | padding-right: 10px;
| + | |
| − | border-radius: 10px;
| + | |
| − | z-index: 30;
| + | |
| − | }
| + | |
| | | | |
| − | #clickme {
| + | <br> |
| − | position: absolute;
| + | <br> |
| − | top: 0;
| + | <br> |
| − | left: 0;
| + | <br> |
| − | height: 300px;
| + | <br> |
| − | width: 50px;
| + | <br> |
| − | background: #FFBA49;
| + | </div> |
| − | z-index: 30;
| + | </div> |
| − | border-radius: 25px 5px 5px 25px;
| + | |
| − | }
| + | |
| − | | + | |
| − | #slidecontent {
| + | |
| − | float: left;
| + | |
| − | }
| + | |
| − | | + | |
| − | #slidecontentco {
| + | |
| − | float: left;
| + | |
| − | }
| + | |
| − | | + | |
| − | .vertical-text {
| + | |
| − | transform: rotate(90deg);
| + | |
| − | transform-origin: left bottom 0;
| + | |
| − | float: left;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* The switch - the box around the slider */
| + | |
| − | .switch {
| + | |
| − | position: relative;
| + | |
| − | display: inline-block;
| + | |
| − | width: 60px;
| + | |
| − | height: 34px;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* Hide default HTML checkbox */
| + | |
| − | .switch input {
| + | |
| − | display: none;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* The slider */
| + | |
| − | .slider {
| + | |
| − | position: absolute;
| + | |
| − | cursor: pointer;
| + | |
| − | top: 0;
| + | |
| − | left: 0;
| + | |
| − | right: 0;
| + | |
| − | bottom: 0;
| + | |
| − | background-color: #ccc;
| + | |
| − | -webkit-transition: .4s;
| + | |
| − | transition: .4s;
| + | |
| − | }
| + | |
| − | | + | |
| − | .slider:before {
| + | |
| − | position: absolute;
| + | |
| − | content: "";
| + | |
| − | height: 26px;
| + | |
| − | width: 26px;
| + | |
| − | left: 4px;
| + | |
| − | bottom: 4px;
| + | |
| − | background-color: white;
| + | |
| − | -webkit-transition: .4s;
| + | |
| − | transition: .4s;
| + | |
| − | }
| + | |
| − | | + | |
| − | input:checked + .slider.bluecolorbutton {
| + | |
| − | background-color: #2196F3;
| + | |
| − | }
| + | |
| − | | + | |
| − | input:focus + .slider.bluecolorbutton {
| + | |
| − | box-shadow: 0 0 1px #2196F3;
| + | |
| − | }
| + | |
| − | | + | |
| − | input:checked + .slider.redcolorbutton {
| + | |
| − | background-color: #FF0000;
| + | |
| − | }
| + | |
| − | | + | |
| − | input:focus + .slider.redcolorbutton {
| + | |
| − | box-shadow: 0 0 1px #FF0000;
| + | |
| − | }
| + | |
| − | | + | |
| − | input:checked + .slider:before {
| + | |
| − | -webkit-transform: translateX(26px);
| + | |
| − | -ms-transform: translateX(26px);
| + | |
| − | transform: translateX(26px);
| + | |
| − | }
| + | |
| − | | + | |
| − | /* Rounded sliders */
| + | |
| − | .slider.round {
| + | |
| − | border-radius: 34px;
| + | |
| − | }
| + | |
| − | | + | |
| − | .slider.round:before {
| + | |
| − | border-radius: 50%;
| + | |
| − | }
| + | |
| − | | + | |
| − | input[type=text] {
| + | |
| − | width: 100%;
| + | |
| − | padding: 12px 20px;
| + | |
| − | margin: 8px 0;
| + | |
| − | box-sizing: border-box;
| + | |
| − | border: 3px solid #ccc;
| + | |
| − | -webkit-transition: 0.5s;
| + | |
| − | transition: 0.5s;
| + | |
| − | outline: none;
| + | |
| − | }
| + | |
| − | | + | |
| − | input[type=text]:focus {
| + | |
| − | border: 3px solid #555;
| + | |
| − | }
| + | |
| − | </style> | + | |
| − | <script> | + | |
| − | $(function () {
| + | |
| − | var rightVal = -150;
| + | |
| − | | + | |
| − | $("#clickme").click(function () {
| + | |
| − | | + | |
| − | rightVal = (rightVal * -1) - 150; | + | |
| − | $(this).parent().animate({right: rightVal + 'px'}, {queue: false, duration: 500}); | + | |
| − | }); | + | |
| − | | + | |
| − | }); | + | |
| − | | + | |
| − | | + | |
| − | </script> | + | |
| | | | |
| | + | <div class="backgroundpic"> |
| | + | <img src="https://static.igem.org/mediawiki/2017/6/62/%E8%83%8C%E6%99%AF1.jpeg" width="100%" |
| | + | height="100%" id="bg" alt=""> |
| | + | </div> |
| | + | </div> |
| | | | |
| | </body> | | </body> |
| | | | |
| | </html> | | </html> |
