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| | <div class="container" style='z-index:10;position:relative;'> | | <div class="container" style='z-index:10;position:relative;'> |
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| − | <nav class="navbar navbar-default">
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| − | <!-- Brand and toggle get grouped for better mobile display -->
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| − | <div class="navbar-header">
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| − | <button type="button" class="navbar-toggle collapsed" data-toggle="collapse"
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| − | data-target="#bs-example-navbar-collapse-1" aria-expanded="false">
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| − | <span class="sr-only">Toggle navigation</span>
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| − | <span class="icon-bar"></span>
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| − | <span class="icon-bar"></span>
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| − | <span class="icon-bar"></span>
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| − | <a class="navbar-brand" href="#">Brand</a>
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| − | </div>
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| − | <!-- Collect the nav links, forms, and other content for toggling -->
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| − | <div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1">
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| − | <ul class="nav navbar-nav">
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| − | <li><a href="#">Link</a></li>
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| − | <li><a href="#">Link</a></li>
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| − | <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true"
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| − | aria-expanded="false">Dropdown <span class="caret"></span></a>
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| − | <ul class="dropdown-menu">
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| − | <li><a href="#">Action</a></li>
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| − | <li><a href="#">Another action</a></li>
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| − | <li><a href="#">Something else here</a></li>
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| − | <li role="separator" class="divider"></li>
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| − | <li><a href="#">Separated link</a></li>
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| − | <li role="separator" class="divider"></li>
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| − | <li><a href="#">One more separated link</a></li>
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| − | <form class="navbar-form navbar-left">
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| − | <div class="form-group">
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| − | <input type="text" class="form-control" placeholder="Search">
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| − | <button type="submit" class="btn btn-default">Submit</button>
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| − | </form>
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| − | <ul class="nav navbar-nav navbar-right">
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| − | <li><a href="#">Link</a></li>
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| − | <li class="dropdown">
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| − | <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true"
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| − | aria-expanded="false">Dropdown <span class="caret"></span></a>
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| − | <li><a href="#">Action</a></li>
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| − | <li role="separator" class="divider"></li>
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| − | </div><!-- /.navbar-collapse -->
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| − | </nav>
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| − |
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| − | <!--
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| | <div class="row card" id='bodycontainer' class='row'> | | <div class="row card" id='bodycontainer' class='row'> |
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| − | <div class="col-sm-2" style='padding-left:5% padding-right:10%'> | + | <nav class="navbar navbar-default navbar-static-top"> |
| − | <div id="category_navbar"> | + | <!-- Brand and toggle get grouped for better mobile display --> |
| − | <ul class="nav nav-list" data-spy="affix" data-offset-top="160" | + | <div class="navbar-header"> |
| − | style='-webkit-transform: translateZ(0);width:160px;margin-left:0'> | + | <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" |
| | + | data-target="#bs-example-navbar-collapse-1" aria-expanded="false"> |
| | + | <span class="sr-only">Toggle navigation</span> |
| | + | <span class="icon-bar"></span> |
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| | + | <a class="navbar-brand" href="#"><img |
| | + | src="https://static.igem.org/mediawiki/2017/8/8c/%E5%91%80%E5%95%8A%E5%93%88.jpeg" width="100"></a> |
| | + | </div> |
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| − | <li><a href="#Background">Background</a></li> | + | <!-- Collect the nav links, forms, and other content for toggling --> |
| − | <li><a href="#Purpose">Purpose</a></li> | + | <div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1"> |
| − | <li><a href="#Overview">Overview of the project</a></li> | + | <ul class="nav navbar-nav"> |
| − | <li><a href="#Goal">Our goal</a></li> | + | <li><a href="/Team:SDSZ-China">Home</a></li> |
| | + | <li><a href="/Team:SDSZ-China/Project">Project</a></li> |
| | + | <li><a href="/Team:SDSZ-China/Experiment">Experiment</a></li> |
| | + | <li><a href="/Team:SDSZ-China/Parts">Parts</a></li> |
| | + | <li><a href="/Team:SDSZ-China/Human_Practices">Human Practices</a></li> |
| | + | <li><a href="/Team:SDSZ-China/Safety">Safety</a></li> |
| | + | <li><a href="/Team:SDSZ-China/Hardware"> Hardware </a></li> |
| | | | |
| | </ul> | | </ul> |
| − | </div> | + | <ul class="nav navbar-nav navbar-right"> |
| | + | <li><a href="/Team:SDSZ-China/Team">Team and attributions</a></li> |
| | + | <li><a href="#">By SDSZ</a></li> |
| | + | </ul> |
| | + | </div><!-- /.navbar-collapse --> |
| | + | </nav> |
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| − | </div>
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| − | <div class="col-sm-10" style="padding-right:5%"> | + | <div class="col-sm-12" style="padding-right:5%"> |
| − | <div class="row">
| + | |
| − | <div class="col-sm-12">
| + | |
| − | <h2 id='Background'>Background</h2>
| + | |
| − | <p>Bear bile, one of the most famous animal drugs in Traditional Chinese Medicine (TCM), has been
| + | |
| − | recorded in ancient Chinese medicine book as a significant method to treat hepatic and biliary
| + | |
| − | disorders. UDCA, the effective ingredient of bear bile.Aside from the traditional use of bear
| + | |
| − | bile in Chinese medicine, UDCA(ursodeoxycholic acid), the effective ingredient of bear bile
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| − | acid, has a much larger pharmaceutical application. As well as the usage of UDCA in dissolving
| + | |
| − | gallstone, its efficacy in primary biliary cirrhosis and primary sclerosing cholangitis (PSC) as
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| − | an adjunct to medical therapy has been well established. Newer indications include its use in
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| − | the management of chronic hepatitis, cirrhosis, post liver transplant rejection,
| + | |
| − | graft-versus-host disease and acute viral hepatitis, where it not only relieves symptoms of
| + | |
| − | cholestasis but also arrests ongoing hepatocyte necrosis. However, the increasing demand for
| + | |
| − | bear bile has caused bears to be in an endangered state: bear poaching and illegal animal trade
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| − | have greatly dwindled the number of the wild Asiatic black bear. Apart from that, bear bile
| + | |
| − | farming industry in Asia extracts bile through “milking” from the bears, which is operated
| + | |
| − | through surgically implanting a permanent catheter in the animal's gallbladder to obtain the
| + | |
| − | drips. It is unquestionable that the bear bile farming process will lead to both physical and
| + | |
| − | psychological damage in bears. </p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| | | | |
| − | <div class="row"> | + | <br> |
| − | <div class="col-sm-12">
| + | <br> |
| − | <h2 id='Purpose'>Purpose</h2>
| + | |
| − | <p>To find substitute or alternative for bear bile farming, our team will be working on the
| + | |
| − | biological synthesis of the main effective component of this important medicine,
| + | |
| − | UDCA(Ursodeoxycholic Acid). This biological approach will not only be more efficient but also be
| + | |
| − | cheaper than the original chemical approach, which is widely used in the current UDCA synthesis
| + | |
| − | industry. </p>
| + | |
| − | </div>
| + | |
| − | </div> | + | |
| | | | |
| − | <div class="row"> | + | <div class="text-center"> |
| − | <div class="col-sm-12">
| + | <h1 class="text-danger">SDSZ - China</h1> |
| − | <h2 id='Overview'>Overview of the project</h2>
| + | <h4 class="text-default">Automatic Synthesize of UDCA(effective ingredient of bear bile) using immobilized enzymes.</h4> |
| − | <p>We found that it is possible to convert the main component of poultry bile, CDCA(Chenodeoxycholic | + | |
| − | Acid), into UDCA, by employing two enzyme-catalyzed the reactions. First, two enzymes was
| + | |
| − | employed to manage the transformation of CDCA to 7oxo-LCA. In the present of
| + | |
| − | 7a-HSD(7alpha-hydroxysteroid dehydrogenase), CDCA is transformed in to 7oxo-LCA by loosing a
| + | |
| − | pair of hydrogen(2H+ and 2e-), the pair of hydrogen is added to NAD+, the cofactor and the
| + | |
| − | acceptor. The NAD+ is transformed into NADH during the reaction. To regenerate the NAD+ and
| + | |
| − | recycle the reaction, to , the LDH(Lactate dehydrogenase) works on pyruvate and take the pair of
| + | |
| − | hydrogen from NADH and transform the pyruvate to lactate and NADH to NAD+.</p>
| + | |
| − | <p>In the second step, the 7oxo-LCA is transformed to UDCA by 7β-HSDH(7beta-hydroxysteroid
| + | |
| − | dehydrogenase)and GDH(glutamate dehydrogenase).The 7β-HSDH works on 7oxo-LCA and take a pair of
| + | |
| − | hydrogen from NADPH(the cofactor for the second step)and add it to 7oxo-LCA and form a beta
| + | |
| − | position 7-hydroxyl group, which is our target product UDCA. The GDH works on glucose and take a
| + | |
| − | pair of hydrogen from it and add it to the NADP+, to form NADPH to manage the regeneration of
| + | |
| − | cofactor NADPH for the second step.</p>
| + | |
| − | </div>
| + | |
| | </div> | | </div> |
| | | | |
| − | <div class="row"> | + | <br> |
| − | <div class="col-sm-12">
| + | <br> |
| − | <h2 id='Goal'>Our goal</h2>
| + | <br> |
| − | <p>Our mission is to expression of the four enzymes 7α-HSDH (from ecoli DH5a), 7β-HSDH (from
| + | |
| − | Ruminococcus Torques), GDH (from Bacillus subtilis), and LDH from (Lactobacillus delbruechii
| + | |
| − | subsp. Bulgaricus)and test their activities. By adding the CBD( cellulose binding
| + | |
| − | domain)sequnence to the plasmid we construct, we manage to bind our enzyme on gauze. This
| + | |
| − | specific design excels in 3 specific ways: first, by controlling the presence of the gauze in
| + | |
| − | the solution, we can control the process of the reaction. Second, when the target enzyme is
| + | |
| − | bound to cellulose we manage to purify the protein we express. Third, the enzyme binding gauze
| + | |
| − | is employed to a machine including the reaction efficiency measuring system and the enzyme
| + | |
| − | addition controlling system .</p>
| + | |
| − | </div>
| + | |
| − | </div> | + | |
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| | <br> | | <br> |
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| | <div class="backgroundpic"> | | <div class="backgroundpic"> |
| − | <img src="https://static.igem.org/mediawiki/2016/e/e5/T--TAS_Taipei--Taipei_Background_1.0.jpg" width="100%" | + | <img src="https://static.igem.org/mediawiki/2017/6/62/%E8%83%8C%E6%99%AF1.jpeg" width="100%" |
| | height="100%" id="bg" alt=""> | | height="100%" id="bg" alt=""> |
| | </div> | | </div> |
