Difference between revisions of "Team:SDSZ-China"

 
(34 intermediate revisions by 3 users not shown)
Line 41: Line 41:
 
         }
 
         }
  
−
         .bgwidth {
+
         #bodycontainer {
−
             width: 100%;
+
             background: rgba(255, 255, 255, 0.5);
−
        }
+
−
 
+
−
        .bgheight {
+
−
            height: 100%;
+
 
         }
 
         }
 
     </style>
 
     </style>
Line 64: Line 60:
 
             src="https://cdn.mathjax.org/mathjax/latest/MathJax.js?config=TeX-MML-AM_CHTML"></script>
 
             src="https://cdn.mathjax.org/mathjax/latest/MathJax.js?config=TeX-MML-AM_CHTML"></script>
 
     <script type="text/x-mathjax-config">
 
     <script type="text/x-mathjax-config">
−
        MathJax.Hub.Config({tex2jax: {inlineMath: [['$','$'], ['\\(','\\)']]}});
+
MathJax.Hub.Config({tex2jax: {inlineMath: [['$','$'], ['\\(','\\)']]}});
  
  
  
 +
 
     </script>
 
     </script>
  
Line 104: Line 101:
  
 
<div class="container" style='z-index:10;position:relative;'>
 
<div class="container" style='z-index:10;position:relative;'>
−
 
−
    <nav class="navbar navbar-inverse">
 
−
        <!-- Brand and toggle get grouped for better mobile display -->
 
−
        <div class="navbar-header">
 
−
            <button type="button" class="navbar-toggle collapsed" data-toggle="collapse"
 
−
                    data-target="#bs-example-navbar-collapse-1" aria-expanded="false">
 
−
                <span class="sr-only">Toggle navigation</span>
 
−
                <span class="icon-bar"></span>
 
−
                <span class="icon-bar"></span>
 
−
                <span class="icon-bar"></span>
 
−
            </button>
 
−
            <a class="navbar-brand" href="#">Brand</a>
 
−
        </div>
 
−
 
−
        <!-- Collect the nav links, forms, and other content for toggling -->
 
−
        <div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1">
 
−
            <ul class="nav navbar-nav">
 
−
                <li><a href="#">Link</a></li>
 
−
                <li><a href="#">Link</a></li>
 
−
            </ul>
 
−
            <form class="navbar-form navbar-left">
 
−
                <div class="form-group">
 
−
                    <input type="text" class="form-control" placeholder="Search">
 
−
                </div>
 
−
                <button type="submit" class="btn btn-default">Submit</button>
 
−
            </form>
 
−
            <ul class="nav navbar-nav navbar-right">
 
−
                <li><a href="#">Link</a></li>
 
−
            </ul>
 
−
        </div><!-- /.navbar-collapse -->
 
−
    </nav>
 
  
  
 
     <div class="row card" id='bodycontainer' class='row'>
 
     <div class="row card" id='bodycontainer' class='row'>
  
−
         <div class="col-sm-2" style='padding-left:5% padding-right:10%'>
+
         <nav class="navbar navbar-default navbar-static-top">
−
             <div id="category_navbar">
+
            <!-- Brand and toggle get grouped for better mobile display -->
−
                 <ul class="nav nav-list" data-spy="affix" data-offset-top="160"
+
             <div class="navbar-header">
−
                     style='-webkit-transform: translateZ(0);width:160px;margin-left:0'>
+
                 <button type="button" class="navbar-toggle collapsed" data-toggle="collapse"
 +
                        data-target="#bs-example-navbar-collapse-1" aria-expanded="false">
 +
                     <span class="sr-only">Toggle navigation</span>
 +
                    <span class="icon-bar"></span>
 +
                    <span class="icon-bar"></span>
 +
                    <span class="icon-bar"></span>
 +
                </button>
 +
                <a class="navbar-brand" href="#"><img
 +
                        src="https://static.igem.org/mediawiki/2017/8/8c/%E5%91%80%E5%95%8A%E5%93%88.jpeg" width="100"></a>
 +
            </div>
  
−
                     <li><a href="#Background">Background</a></li>
+
            <!-- Collect the nav links, forms, and other content for toggling -->
−
                     <li><a href="#Purpose">Purpose</a></li>
+
            <div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1">
−
                     <li><a href="#Overview">Overview of the project</a></li>
+
                <ul class="nav navbar-nav">
−
                     <li><a href="#Goal">Our goal</a></li>
+
                     <li><a href="/Team:SDSZ-China">Home</a></li>
 +
                     <li><a href="/Team:SDSZ-China/Project">Project</a></li>
 +
                     <li><a href="/Team:SDSZ-China/Experiment">Experiment</a></li>
 +
                     <li><a href="/Team:SDSZ-China/Parts">Parts</a></li>
 +
                    <li><a href="/Team:SDSZ-China/Human_Practices">Human Practices</a></li>
 +
                    <li><a href="/Team:SDSZ-China/Safety">Safety</a></li>
 +
                    <li><a href="/Team:SDSZ-China/Hardware"> Hardware </a></li>
  
 
                 </ul>
 
                 </ul>
−
             </div>
+
                <ul class="nav navbar-nav navbar-right">
 +
                    <li><a href="/Team:SDSZ-China/Team">Team and attributions</a></li>
 +
                    <li><a href="#">By SDSZ</a></li>
 +
                </ul>
 +
             </div><!-- /.navbar-collapse -->
 +
        </nav>
  
−
        </div>
 
  
−
         <div class="col-sm-10" style="padding-right:5%">
+
         <div class="col-sm-12" style="padding-right:5%">
−
            <div class="row">
+
−
                <div class="col-sm-12">
+
−
                    <h2 id='Background'>Background</h2>
+
−
                    <p>Bear bile, one of the most famous animal drugs in Traditional Chinese Medicine (TCM), has been
+
−
                        recorded in ancient Chinese medicine book as a significant method to treat hepatic and biliary
+
−
                        disorders. UDCA, the effective ingredient of bear bile.Aside from the traditional use of bear
+
−
                        bile in Chinese medicine, UDCA(ursodeoxycholic acid), the effective ingredient of bear bile
+
−
                        acid, has a much larger pharmaceutical application. As well as the usage of UDCA in dissolving
+
−
                        gallstone, its efficacy in primary biliary cirrhosis and primary sclerosing cholangitis (PSC) as
+
−
                        an adjunct to medical therapy has been well established. Newer indications include its use in
+
−
                        the management of chronic hepatitis, cirrhosis, post liver transplant rejection,
+
−
                        graft-versus-host disease and acute viral hepatitis, where it not only relieves symptoms of
+
−
                        cholestasis but also arrests ongoing hepatocyte necrosis. However, the increasing demand for
+
−
                        bear bile has caused bears to be in an endangered state: bear poaching and illegal animal trade
+
−
                        have greatly dwindled the number of the wild Asiatic black bear. Apart from that, bear bile
+
−
                        farming industry in Asia extracts bile through “milking” from the bears, which is operated
+
−
                        through surgically implanting a permanent catheter in the animal's gallbladder to obtain the
+
−
                        drips. It is unquestionable that the bear bile farming process will lead to both physical and
+
−
                        psychological damage in bears. </p>
+
−
                </div>
+
−
            </div>
+
  
−
             <div class="row">
+
             <br>
−
                <div class="col-sm-12">
+
             <br>
−
                    <h2 id='Purpose'>Purpose</h2>
+
−
                    <p>To find substitute or alternative for bear bile farming, our team will be working on the
+
−
                        biological synthesis of the main effective component of this important medicine,
+
−
                        UDCA(Ursodeoxycholic Acid). This biological approach will not only be more efficient but also be
+
−
                        cheaper than the original chemical approach, which is widely used in the current UDCA synthesis
+
−
                        industry. </p>
+
−
                </div>
+
−
             </div>
+
  
−
             <div class="row">
+
             <div class="text-center">
−
                <div class="col-sm-12">
+
                    <h1 class="text-danger">SDSZ - China</h1>
−
                    <h2 id='Overview'>Overview of the project</h2>
+
                     <h4 class="text-default">Automatic Synthesize of UDCA(effective ingredient of bear bile) using immobilized enzymes.</h4>
−
                     <p>We found that it is possible to convert the main component of poultry bile, CDCA(Chenodeoxycholic
+
−
                        Acid), into UDCA, by employing two enzyme-catalyzed the reactions. First, two enzymes was
+
−
                        employed to manage the transformation of CDCA to 7oxo-LCA. In the present of
+
−
                        7a-HSD(7alpha-hydroxysteroid dehydrogenase), CDCA is transformed in to 7oxo-LCA by loosing a
+
−
                        pair of hydrogen(2H+ and 2e-), the pair of hydrogen is added to NAD+, the cofactor and the
+
−
                        acceptor. The NAD+ is transformed into NADH during the reaction. To regenerate the NAD+ and
+
−
                        recycle the reaction, to , the LDH(Lactate dehydrogenase) works on pyruvate and take the pair of
+
−
                        hydrogen from NADH and transform the pyruvate to lactate and NADH to NAD+.</p>
+
−
                    <p>In the second step, the 7oxo-LCA is transformed to UDCA by 7β-HSDH(7beta-hydroxysteroid
+
−
                        dehydrogenase)and GDH(glutamate dehydrogenase).The 7β-HSDH works on 7oxo-LCA and take a pair of
+
−
                        hydrogen from NADPH(the cofactor for the second step)and add it to 7oxo-LCA and form a beta
+
−
                        position 7-hydroxyl group, which is our target product UDCA. The GDH works on glucose and take a
+
−
                        pair of hydrogen from it and add it to the NADP+, to form NADPH to manage the regeneration of
+
−
                        cofactor NADPH for the second step.</p>
+
−
                </div>
+
 
             </div>
 
             </div>
  
−
             <div class="row">
+
             <br>
−
                <div class="col-sm-12">
+
            <br>
−
                    <h2 id='Goal'>Our goal</h2>
+
             <br>
−
                    <p>Our mission is to expression of the four enzymes 7α-HSDH (from ecoli DH5a), 7β-HSDH (from
+
−
                        Ruminococcus Torques), GDH (from Bacillus subtilis), and LDH from (Lactobacillus delbruechii
+
−
                        subsp. Bulgaricus)and test their activities. By adding the CBD( cellulose binding
+
−
                        domain)sequnence to the plasmid we construct, we manage to bind our enzyme on gauze. This
+
−
                        specific design excels in 3 specific ways: first, by controlling the presence of the gauze in
+
−
                        the solution, we can control the process of the reaction. Second, when the target enzyme is
+
−
                        bound to cellulose we manage to purify the protein we express. Third, the enzyme binding gauze
+
−
                        is employed to a machine including the reaction efficiency measuring system and the enzyme
+
−
                        addition controlling system .</p>
+
−
                </div>
+
−
             </div>
+
 
             <br>
 
             <br>
 
             <br>
 
             <br>
Line 229: Line 159:
  
 
     <div class="backgroundpic">
 
     <div class="backgroundpic">
−
         <img src="https://static.igem.org/mediawiki/2016/e/e5/T--TAS_Taipei--Taipei_Background_1.0.jpg" width="100%"
+
         <img src="https://static.igem.org/mediawiki/2017/6/62/%E8%83%8C%E6%99%AF1.jpeg" width="100%"
 
             height="100%" id="bg" alt="">
 
             height="100%" id="bg" alt="">
 
     </div>
 
     </div>

Latest revision as of 03:24, 2 November 2017


Project



SDSZ - China

Automatic Synthesize of UDCA(effective ingredient of bear bile) using immobilized enzymes.