Difference between revisions of "Team:SDSZ-China"

 
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                     <li><a href="/Team:SDSZ-China">Home</a></li>
 
                     <li><a href="/Team:SDSZ-China">Home</a></li>
 
                     <li><a href="/Team:SDSZ-China/Project">Project</a></li>
 
                     <li><a href="/Team:SDSZ-China/Project">Project</a></li>
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                    <li><a href="/Team:SDSZ-China/Experiment">Experiment</a></li>
 
                     <li><a href="/Team:SDSZ-China/Parts">Parts</a></li>
 
                     <li><a href="/Team:SDSZ-China/Parts">Parts</a></li>
 
                     <li><a href="/Team:SDSZ-China/Human_Practices">Human Practices</a></li>
 
                     <li><a href="/Team:SDSZ-China/Human_Practices">Human Practices</a></li>
 
                     <li><a href="/Team:SDSZ-China/Safety">Safety</a></li>
 
                     <li><a href="/Team:SDSZ-China/Safety">Safety</a></li>
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                    <li><a href="/Team:SDSZ-China/Hardware"> Hardware </a></li>
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                     <li><a href="/Team:SDSZ-China/Team">Team</a></li>
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                     <li><a href="/Team:SDSZ-China/Team">Team and attributions</a></li>
 
                     <li><a href="#">By SDSZ</a></li>
 
                     <li><a href="#">By SDSZ</a></li>
 
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                    <li><a href="#Background">Background</a></li>
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                    <li><a href="#Purpose">Purpose</a></li>
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                    <li><a href="#Overview">Overview of the project</a></li>
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                    <h1 class="text-danger">SDSZ - China</h1>
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                    <h4 class="text-default">Automatic Synthesize of UDCA(effective ingredient of bear bile) using immobilized enzymes.</h4>
 
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                    <h2 id='Background'>Background</h2>
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                    <p>Bear bile, one of the most famous animal drugs in Traditional Chinese Medicine (TCM), has been
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                        recorded in ancient Chinese medicine book as a significant method to treat hepatic and biliary
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                        disorders. UDCA, the effective ingredient of bear bile.Aside from the traditional use of bear
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                        bile in Chinese medicine, UDCA(ursodeoxycholic acid), the effective ingredient of bear bile
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                        acid, has a much larger pharmaceutical application. As well as the usage of UDCA in dissolving
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                        gallstone, its efficacy in primary biliary cirrhosis and primary sclerosing cholangitis (PSC) as
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                        an adjunct to medical therapy has been well established. Newer indications include its use in
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                        the management of chronic hepatitis, cirrhosis, post liver transplant rejection,
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                        graft-versus-host disease and acute viral hepatitis, where it not only relieves symptoms of
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                        cholestasis but also arrests ongoing hepatocyte necrosis. However, the increasing demand for
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                        bear bile has caused bears to be in an endangered state: bear poaching and illegal animal trade
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                        have greatly dwindled the number of the wild Asiatic black bear. Apart from that, bear bile
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                        farming industry in Asia extracts bile through “milking” from the bears, which is operated
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                        through surgically implanting a permanent catheter in the animal's gallbladder to obtain the
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                        drips. It is unquestionable that the bear bile farming process will lead to both physical and
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                        psychological damage in bears. </p>
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                    <h2 id='Purpose'>Purpose</h2>
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                    <p>To find substitute or alternative for bear bile farming, our team will be working on the
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                        biological synthesis of the main effective component of this important medicine,
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                        UDCA(Ursodeoxycholic Acid). This biological approach will not only be more efficient but also be
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                        cheaper than the original chemical approach, which is widely used in the current UDCA synthesis
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                        industry. </p>
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                    <h2 id='Overview'>Overview of the project</h2>
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                    <p>We found that it is possible to convert the main component of poultry bile, CDCA(Chenodeoxycholic
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                        Acid), into UDCA, by employing two enzyme-catalyzed the reactions. First, two enzymes was
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                        employed to manage the transformation of CDCA to 7oxo-LCA. In the present of
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                        7a-HSD(7alpha-hydroxysteroid dehydrogenase), CDCA is transformed in to 7oxo-LCA by loosing a
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                        pair of hydrogen(2H+ and 2e-), the pair of hydrogen is added to NAD+, the cofactor and the
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                        acceptor. The NAD+ is transformed into NADH during the reaction. To regenerate the NAD+ and
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                        recycle the reaction, to , the LDH(Lactate dehydrogenase) works on pyruvate and take the pair of
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                        hydrogen from NADH and transform the pyruvate to lactate and NADH to NAD+.</p>
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                    <p>In the second step, the 7oxo-LCA is transformed to UDCA by 7β-HSDH(7beta-hydroxysteroid
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                        dehydrogenase)and GDH(glutamate dehydrogenase).The 7β-HSDH works on 7oxo-LCA and take a pair of
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                        hydrogen from NADPH(the cofactor for the second step)and add it to 7oxo-LCA and form a beta
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                        position 7-hydroxyl group, which is our target product UDCA. The GDH works on glucose and take a
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                        pair of hydrogen from it and add it to the NADP+, to form NADPH to manage the regeneration of
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                        cofactor NADPH for the second step.</p>
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                    <h2 id='Goal'>Our goal</h2>
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                    <p>Our mission is to expression of the four enzymes 7α-HSDH (from ecoli DH5a), 7β-HSDH (from
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                        Ruminococcus Torques), GDH (from Bacillus subtilis), and LDH from (Lactobacillus delbruechii
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                        subsp. Bulgaricus)and test their activities. By adding the CBD( cellulose binding
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                        domain)sequnence to the plasmid we construct, we manage to bind our enzyme on gauze. This
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                        specific design excels in 3 specific ways: first, by controlling the presence of the gauze in
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                        the solution, we can control the process of the reaction. Second, when the target enzyme is
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                        bound to cellulose we manage to purify the protein we express. Third, the enzyme binding gauze
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                        is employed to a machine including the reaction efficiency measuring system and the enzyme
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                        addition controlling system .</p>
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Latest revision as of 03:24, 2 November 2017


Project



SDSZ - China

Automatic Synthesize of UDCA(effective ingredient of bear bile) using immobilized enzymes.