Difference between revisions of "Team:UCopenhagen/Parts"

 
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                     <div class="intro-message2">
 
                     <div class="intro-message2">
 
<h3></h3>
 
<h3></h3>
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                         <h1>I N T E R  L A B</h1>   
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                         <h1> P A R T S</h1>   
 
                     </div>
 
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                 </div>
 
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                     <div class="clearfix"></div>
 
                     <div class="clearfix"></div>
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                     <h2 class="section-heading">Cell measurements </h2>
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                     <h2 class="section-heading">Overview of our Biobricks </h2>
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                     <p class="lead">Two colonies from each transformation were picked, and grown in foil-covered 50 ml falcon tubes over night (18 hours). </p>
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                     <p class="lead">
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<p class="lead"><strong>Preparation</strong> OD was measured, and a dilution was calculated to achieve an OD of 0.02. Dilution calculations can be found in the table next to this. Here we used the calculated correction factor from our initial abs/OD calibration. From the absorption measurements taken at 0 hours, we see indications of pipetting errors, as the OD<sub>600</sub> (average) ranges from 0.015 to 0.6 (table 1). </p>
+
All our submitted Biobricks are listed on this page. Special attention should be directed to our two favorite parts: 
 +
<li>
 +
                <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2455003">BBa_K2455003</a>, a Cell-Penetrating USER Cassette, which is completely novel in iGEM. We believe this brick can benefit many iGEM teams in the years to come, as we have made it easy to insert any protein of interest in the USER casette. We have already used it ourselves, to create our second favorite part.
 +
</li>
 +
<li>
 +
                <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2455002">BBa_K2455002</a>, an improved version of the super yellow fluorescent protein <a href="http://parts.igem.org/Part:BBa_K864100">BBa_K864100</a> that we believe fulfills the gold medal criteria to improve a previous part or project.  
 +
</li>
 +
 
 +
</p>    </p>
  
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<button onclick="javascript:showhide('InterLab-table')">Show table 1</button>
 
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<div id="InterLab-table" style="display:none;">
 
 
<div class="container">
 
<div class="container">
 
   <table class="responsive-table">
 
   <table class="responsive-table">
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    <caption><b>Table 1</b> Starting OD.</caption>
 
 
     <thead>
 
     <thead>
 
       <tr>
 
       <tr>
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<th scope="col"></th>
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        <th scope="col">Name</th>
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         <th scope="col">Abs<sub>600</sub></th>
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         <th scope="col">Type</th>
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         <th scope="col">OD<sub>600</sub> before</th>
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         <th scope="col">Type</th>
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         <th scope="col">Volume (ml) added to 12 ml media</th>
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         <th scope="col">Designer</th>
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        <th scope="col">OD<sub>600</sub> after dilution (0 hours)</th>
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<th scope="col">Length</th>
 
       </tr>
 
       </tr>
 
     </thead>
 
     </thead>
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     <tbody>
 
     <tbody>
 
       <tr>
 
       <tr>
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         <th scope="row">Negative Control (Colony 1)</th>
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         <th scope="row"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2455000" style="color:black">BBa_K2455000</a></th>
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         <td data-title="Abs600">0,461</td>
+
         <td data-title="Type">Basic Part</td>
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         <td data-title="OD600 before">1,435</td>
+
         <td data-title="Description">His-tagged AroG gene from E. coli MG1655</td>
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         <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,167</td>
+
         <td data-title="Designer" data-type="currency">Jon Fugl</td>
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         <td data-title="OD600 after dilution (0 hours)" data-type="currency">0,06483974</td>
+
         <td data-title="Length" data-type="currency">1092</td>
 
       </tr>
 
       </tr>
−
      <tr>
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  <tr>
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         <th scope="row">Negative Control (Colony 2)</th>
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         <th scope="row"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2455001" style="color:black">BBa_K2455001</a></th>
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         <td data-title="Abs600">0,463</td>
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         <td data-title="Type">Basic Part</td>
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         <td data-title="OD600 before">1,439</td>
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         <td data-title="Description">His-tagged TrpE gene from E. coli MG1655</td>
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         <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,167</td>
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         <td data-title="Designer" data-type="currency">Jon Fugl</td>
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         <td data-title="OD600 after dilution (0 hours)" data-type="currency">0,02584249</td>
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         <td data-title="Length" data-type="currency">1602</td>
 
       </tr>
 
       </tr>
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        <tr>
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  <tr>
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         <th scope="row">Positive Control (Colony 1)</th>
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         <th scope="row"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2455002" style="color:black">BBa_K2455002</a></th>
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         <td data-title="Abs<sub>600</sub>">0,450</td>
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         <td data-title="Type">Basic Part (Improvement of old)</td>
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         <td data-title="OD<sub>600</sub> before">1,400</td>
+
         <td data-title="Description">R9-SYFP2-6His</td>
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         <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,171</td>
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         <td data-title="Designer" data-type="currency">Jon Fugl</td>
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         <td data-title="OD<sub>600</sub> after dilution (0 hours)" data-type="currency">0,04467949</td>
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         <td data-title="Length" data-type="currency">804</td>
 
       </tr>
 
       </tr>
−
    <tr>
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  <tr>
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         <th scope="row">Positive Control (Colony 2)</th>
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         <th scope="row"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2455003" style="color:black">BBa_K2455003</a></th>
−
         <td data-title="Abs<sub>600</sub>">0,474</td>
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         <td data-title="Type">Basic Part</td>
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         <td data-title="OD<sub>600</sub> before">1,475</td>
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         <td data-title="Description">Nona-Arginine with his-tag</td>
−
         <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,163</td>
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         <td data-title="Designer" data-type="currency">Jon Fugl</td>
−
         <td data-title="OD<sub>600</sub> after dilution (0 hours)" data-type="currency">0,01689103</td>
+
         <td data-title="Length" data-type="currency">81</td>
 
       </tr>
 
       </tr>
−
          <tr>
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  <tr>
−
         <th scope="row">Test Device 1: J23101+I13504 (Colony 1)</th>
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         <th scope="row"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2455004" style="color:black">BBa_K2455004</a></th>
−
         <td data-title="Abs<sub>600</sub>">0,462</td>
+
         <td data-title="Type">Basic Part</td>
−
         <td data-title="OD<sub>600</sub> before">1,437</td>
+
         <td data-title="Description">YddG E. coli Tyr, Trp, Phe exporter </td>
−
         <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,167</td>
+
         <td data-title="Designer" data-type="currency">Jon Fugl</td>
−
         <td data-title="OD<sub>600</sub> after dilution (0 hours)" data-type="currency">0,03043498</td>
+
         <td data-title="Length" data-type="currency">885</td>
 
       </tr>
 
       </tr>
−
        <tr>
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  <tr>
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         <th scope="row">Test Device 1: J23101+I13504 (Colony 2)</th>
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         <th scope="row"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2455005" style="color:black">BBa_K2455005</a></th>
−
         <td data-title="Abs<sub>600</sub>">0,472</td>
+
         <td data-title="Type">Basic Part (Improvement of old - Illegal)</td>
−
         <td data-title="OD<sub>600</sub> before">1,469</td>
+
         <td data-title="Description">R9-mTag BFP-6His </td>
−
         <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,163</td>
+
         <td data-title="Designer" data-type="currency">Jon Fugl</td>
−
         <td data-title="OD<sub>600</sub> after dilution (0 hours)" data-type="currency">0,01977106</td>
+
         <td data-title="Length" data-type="currency">786</td>
 
       </tr>
 
       </tr>
−
        <tr>
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  <tr>
−
         <th scope="row">Test Device 2: J23106+I13504 (Colony 1)</th>
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         <th scope="row"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2455007" style="color:black">BBa_K2455007</a></th>
−
         <td data-title="Abs<sub>600</sub>">0,477</td>
+
         <td data-title="Type">Basic Part (Composite)</td>
−
         <td data-title="OD<sub>600</sub> before">1,483</td>
+
         <td data-title="Description">YddG-TrpE construct with an E. coli RBS between (BBa_K2455004 & BBa_K2455001)</td>
−
         <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,162</td>
+
         <td data-title="Designer" data-type="currency">Jon Fugl</td>
−
         <td data-title="OD<sub>600</sub> after dilution (0 hours)" data-type="currency">0,03175824</td>
+
         <td data-title="Length" data-type="currency">2513</td>
 
       </tr>
 
       </tr>
−
        <tr>
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  <tr>
−
         <th scope="row">Test Device 2: J23106+I13504 (Colony 2)</th>
+
         <th scope="row"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2455008" style="color:black">BBa_K2455008</a></th>
−
        <td data-title="Abs<sub>600</sub>">0,454</td>
+
         <td data-title="Type">Basic Part (Composite)</td>
−
        <td data-title="OD<sub>600</sub> before">1,410</td>
+
         <td data-title="Description">YddG-AroG construct with an E. coli RBS between (BBa_K2455004 & BBa_K2455000) </td>
−
        <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,170</td>
+
         <td data-title="Designer" data-type="currency">Jon Fugl</td>
−
        <td data-title="OD<sub>600</sub> after dilution (0 hours)" data-type="currency">0,018837</td>
+
         <td data-title="Length" data-type="currency">2005</td>
−
      </tr>
+
−
          <tr>
+
−
        <th scope="row">Test Device 3: J23117+I13504 (Colony 1)</th>
+
−
        <td data-title="Abs<sub>600</sub>">0,507</td>
+
−
        <td data-title="OD<sub>600</sub> before">1,577</td>
+
−
        <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,152</td>
+
−
        <td data-title="OD<sub>600</sub> after dilution (0 hours)" data-type="currency">0,03261447</td>
+
−
      </tr>
+
−
          <tr>
+
−
        <th scope="row">Test Device 3: J23117+I13504 (Colony 2)</th>
+
−
        <td data-title="Abs<sub>600</sub>">0,492</td>
+
−
        <td data-title="OD<sub>600</sub> before">1,529</td>
+
−
        <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,157</td>
+
−
        <td data-title="OD<sub>600</sub> after dilution (0 hours)" data-type="currency">0,01471154</td>
+
−
      </tr>
+
−
          <tr>
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−
        <th scope="row">Test Device 4: J23101.BCD2.E0040.B0015 (Colony 1)</th>
+
−
        <td data-title="Abs<sub>600</sub>">0,420</td>
+
−
        <td data-title="OD<sub>600</sub> before">1,307</td>
+
−
        <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,184</td>
+
−
        <td data-title="OD<sub>600</sub> after dilution (0 hours)" data-type="currency">0,03759615</td>
+
−
      </tr>
+
−
          <tr>
+
−
        <th scope="row">Test Device 4: J23101.BCD2.E0040.B0015 (Colony 2)</th>
+
−
        <td data-title="Abs600">0,472</td>
+
−
        <td data-title="OD600 before">1,468</td>
+
−
        <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,163</td>
+
−
        <td data-title="OD600 after dilution (0 hours)" data-type="currency">0,01860348</td>
+
−
      </tr>
+
−
      <tr>
+
−
        <th scope="row">Test Device 5: J23106.BCD2.E0040.B0015 (Colony 1)</th>
+
−
        <td data-title="Abs600">0,513</td>
+
−
        <td data-title="OD600 before">1,595</td>
+
−
        <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,150</td>
+
−
        <td data-title="OD600 after dilution (0 hours)" data-type="currency">0,04234432</td>
+
−
      </tr>
+
−
      <tr>
+
−
        <th scope="row">Test Device 5: J23106.BCD2.E0040.B0015 (Colony 2)</th>
+
−
         <td data-title="Abs600">0,467</td>
+
−
        <td data-title="OD600 before">1,451</td>
+
−
        <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,165</td>
+
−
         <td data-title="OD600 after dilution (0 hours)" data-type="currency">0,01494505</td>
+
−
      </tr>
+
−
      <tr>
+
−
        <th scope="row">Test Device 6: J23117.BCD2.E0040.B0015 (Colony 1)</th>
+
−
        <td data-title="Abs600">0,482</td>
+
−
        <td data-title="OD600 before">1,498</td>
+
−
        <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,160</td>
+
−
        <td data-title="OD600 after dilution (0 hours)" data-type="currency">0,04452381</td>
+
−
      </tr>
+
−
      <tr>
+
−
        <th scope="row">Test Device 6: J23117.BCD2.E0040.B0015 (Colony 2)</th>
+
−
        <td data-title="Abs600">0,455</td>
+
−
         <td data-title="OD600 before">1,416</td>
+
−
        <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,170</td>
+
−
         <td data-title="OD600 after dilution (0 hours)" data-type="currency">0,02008242</td>
+
 
       </tr>
 
       </tr>
 
     </tbody>
 
     </tbody>
 
   </table>
 
   </table>
 
</div>
 
</div>
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</div>
 
   </div>
 
   </div>
 
</div>
 
</div>
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</div>
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</div>
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Latest revision as of 03:25, 2 November 2017

P A R T S

Overview of our Biobricks

All our submitted Biobricks are listed on this page. Special attention should be directed to our two favorite parts:

  • BBa_K2455003, a Cell-Penetrating USER Cassette, which is completely novel in iGEM. We believe this brick can benefit many iGEM teams in the years to come, as we have made it easy to insert any protein of interest in the USER casette. We have already used it ourselves, to create our second favorite part.
  • BBa_K2455002, an improved version of the super yellow fluorescent protein BBa_K864100 that we believe fulfills the gold medal criteria to improve a previous part or project.

    • Name Type Type Designer Length
    BBa_K2455000 Basic Part His-tagged AroG gene from E. coli MG1655 Jon Fugl 1092
    BBa_K2455001 Basic Part His-tagged TrpE gene from E. coli MG1655 Jon Fugl 1602
    BBa_K2455002 Basic Part (Improvement of old) R9-SYFP2-6His Jon Fugl 804
    BBa_K2455003 Basic Part Nona-Arginine with his-tag Jon Fugl 81
    BBa_K2455004 Basic Part YddG E. coli Tyr, Trp, Phe exporter Jon Fugl 885
    BBa_K2455005 Basic Part (Improvement of old - Illegal) R9-mTag BFP-6His Jon Fugl 786
    BBa_K2455007 Basic Part (Composite) YddG-TrpE construct with an E. coli RBS between (BBa_K2455004 & BBa_K2455001) Jon Fugl 2513
    BBa_K2455008 Basic Part (Composite) YddG-AroG construct with an E. coli RBS between (BBa_K2455004 & BBa_K2455000) Jon Fugl 2005

    Find Incell here: