Difference between revisions of "Team:TokyoTech/Experiment/Overview"

 
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     <label for="vmcb-d"><a>Experiment</a></label>
 
     <label for="vmcb-d"><a>Experiment</a></label>
 
     <ul>
 
     <ul>
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         <li style="padding-bottom: 10px; padding-top: 10px">
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         <input type="checkbox" id="vmcb-d1" />
 
         <input type="checkbox" id="vmcb-d1" />
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          <label for="vmcb-d1"><a style="text-align: center;">Bacteria <br>to Human Cells ▼</a></label>
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        <label for="vmcb-d1"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/Overview" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white" style="text-align: center;">Overview</a></label>
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        </li>
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        <li style="padding-bottom: 10px; padding-top: 5px">
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        <input type="checkbox" id="vmcb-d2" />
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          <label for="vmcb-d2"><a style="text-align: center;">Bacteria to <br>Human Cells ▼</a></label>
 
             <ul>
 
             <ul>
 
               <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraI_Assay" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">TraI Assay</a></li>
 
               <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraI_Assay" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">TraI Assay</a></li>
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               <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraI_Improvement" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">TraI Impovement <br>Assay</a></li>
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               <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraI_Improvement" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">TraI Improvement <br>Assay</a></li>
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               <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraR_Reporter_Assay" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white" >TraR Reporter <br> Assay</a></li>
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               <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraR_Reporter_Assay" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white" >TraR Reporter <br>Assay</a></li>
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               <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/Transcriptome_Analysis" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Transcriptome <br> Analysis</a></li>
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               <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/Transcriptome_Analysis" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Transcriptome <br>Analysis</a></li>
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               <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/Chimeric_Transcription_Factor" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Chimeric <br> Transcription <br> Factor Assay</a></li>
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               <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/Chimeric_Transcription_Factor" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Chimeric <br> Transcription <br>Factor Assay</a></li>
 
             </ul>
 
             </ul>
 
       </li>
 
       </li>
  
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     <label for="vmcb-d2"><a style="text-align: center;">Human Cells to Bacteria ▼</a></label>
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     <label for="vmcb-d3"><a style="text-align: center;">Human Cells to <br>Bacteria ▼</a></label>
 
         <ul>
 
         <ul>
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           <li style="font-size: 16px"><a href="https://2017.igem.org/Team:TokyoTech/Experiment/AHK4_Assay" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">AHK4 Assay</a></li>
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           <li><a href="https://2017.igem.org/Team:TokyoTech/Experiment/AHK4_Assay" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">AHK4 Assay</a></li>
 
           </ul>
 
           </ul>
 
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     <label for="vmcb-d3"><a href="https://2017.igem.org/Team:TokyoTech/InterLab" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white" style="text-align: center;">InterLab</a></label>
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     <label for="vmcb-d4"><a href="https://2017.igem.org/Team:TokyoTech/InterLab" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white" style="text-align: center;">InterLab</a></label>
 
         </li>
 
         </li>
 
     </ul>
 
     </ul>
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     <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/HP" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Overview</a></li>
 
     <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/HP" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Overview</a></li>
 
     <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/HP/Silver" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Silver</a></li>
 
     <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/HP/Silver" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Silver</a></li>
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     <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/HP/Gold_Integrated" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Integrated <br> Human Practice</a></li>
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     <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/HP/Gold_Integrated" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Gold (Integrated)</a></li>
 
     <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Demonstrate" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Demonstrate</a></li>
 
     <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Demonstrate" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Demonstrate</a></li>
 
     <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Collaborations" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Collaborations</a></li>
 
     <li style="text-align: center;"><a href="https://2017.igem.org/Team:TokyoTech/Collaborations" onclick="w3_close()" class="w3-bar-item w3-button w3-hover-white">Collaborations</a></li>
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   <h1 class="w3-xxxlarge w3-text-red" style="padding-bottom: 10px;padding-top: 10px"><b>Introduction</b></h1>
 
   <h1 class="w3-xxxlarge w3-text-red" style="padding-bottom: 10px;padding-top: 10px"><b>Introduction</b></h1>
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       <div style="padding: 10px; margin-bottom: 3px; border: 1px dotted #333333;width: 90%; border-radius: 10px">
 
       <div style="padding: 10px; margin-bottom: 3px; border: 1px dotted #333333;width: 90%; border-radius: 10px">
 
       <h4 style="text-align: center">Signal transmission system from bacteria to human cells</h4>
 
       <h4 style="text-align: center">Signal transmission system from bacteria to human cells</h4>
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       <figure>
 
       <figure>
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       <img src="https://static.igem.org/mediawiki/2017/1/15/T--TokyoTech--b_to_h.png" style="max-width:80%">
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       <img src="https://static.igem.org/mediawiki/2017/e/e6/T--TokyoTech--bacteriatohuman.png" style="max-width:80%">
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       <figcaption style="font-size: 16px">Fig. Mechanism of signal transmission system from bacteria to human</figcaption>
+
       <figcaption style="font-size: 16px">Fig. 1 Mechanism of signal transmission system from bacteria to human</figcaption>
 
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         <a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraI_Assay">
 
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         <img src="https://static.igem.org/mediawiki/2016/4/47/Tokyo_Tech_JASSO.gif" style="width:100%">
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         <img src="https://static.igem.org/mediawiki/2017/2/23/Fig3_construction_of_TraI_gene3.png" style="width:100%">
 
         <div class="w3-container">
 
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           <h3>TraI Assay</h3>
 
           <h3>TraI Assay</h3>
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         <img src="https://static.igem.org/mediawiki/2017/1/15/T--TokyoTech--human_cell_circuit.png" style="width:100%">
 
         <div class="w3-container">
 
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           <h3>TraI Impovement <br>Assay</h3>
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           <h3>Chimeric <br> Transcription Factor <br>Assay</h3>
 
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         <a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraR_Reporter_Assay">
 
         <a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraR_Reporter_Assay">
−
         <img src="http://www.vmcs.ucdavis.edu/idt/images/idt-logo.jpg" style="width:100%">
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         <img src="https://static.igem.org/mediawiki/2017/1/14/T--TokyoTech--TraRAssay.png" style="width:100%">
 
         <div class="w3-container">
 
         <div class="w3-container">
 
           <h3>TraR Reporter <br> Assay</h3>
 
           <h3>TraR Reporter <br> Assay</h3>
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       <a href="https://2017.igem.org/Team:TokyoTech/Experiment/Transcriptome_Analysis">
 
       <a href="https://2017.igem.org/Team:TokyoTech/Experiment/Transcriptome_Analysis">
−
         <img src="https://static.igem.org/mediawiki/2016/4/47/Tokyo_Tech_JASSO.gif" style="width:100%">
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         <img src="https://static.igem.org/mediawiki/2017/1/10/T--TokyoTech--Transcriptome_malg2.png" style="width:100%">
 
         <div class="w3-container">
 
         <div class="w3-container">
 
           <h3>Transcriptome <br> Analysis</h3>
 
           <h3>Transcriptome <br> Analysis</h3>
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         <a href="https://2017.igem.org/Team:TokyoTech/Experiment/TraI_Improvement">
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         <img src="http://kuramae-old.tokyo.r-cms.jp/files/user/common/p_images/head_logo.gif" style="width:100%">
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         <img src="https://static.igem.org/mediawiki/2017/d/db/T--TokyoTech--TraIimprove_11021228.png" style="width:100%">
 
         <div class="w3-container">
 
         <div class="w3-container">
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           <h3>Chimeric <br> Transcription <br> Factor Assay</h3>
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           <h3>TraI Improvement <br>Assay</h3>
 
         </div>
 
         </div>
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       </div>
 
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       <br>
  
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       <div style="padding: 10px; margin-bottom: 10px; border: 1px dotted #333333;width: 90%;border-radius: 10px">
 
       <div style="padding: 10px; margin-bottom: 10px; border: 1px dotted #333333;width: 90%;border-radius: 10px">
 
       <h4 style="text-align: center">Signal transmission system from human cells to bacteria</h4>
 
       <h4 style="text-align: center">Signal transmission system from human cells to bacteria</h4>
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       <figure>
 
       <figure>
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       <img src="https://static.igem.org/mediawiki/2017/f/f5/T--TokyoTech--h_to_b.png" style="width:80%">
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       <img src="https://static.igem.org/mediawiki/2017/5/5a/T--TokyoTech--humantobacteria.png" style="width:80%">
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       <figcaption style="font-size: 16px">Fig. Mechanism of signal transmission system from human cells to bacteria</figcaption>
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       <figcaption style="font-size: 16px">Fig. 2 Mechanism of signal transmission system from human cells to bacteria</figcaption>
 
       </figure>
 
       </figure>
 
       </div>
 
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       <div onclick="obj=document.getElementById('menu1').style; obj.display=(obj.display=='none')?'block':'none';" style="text-align: center;" style="margin-bottom: 30px"><button class="w3-button w3-red w3-padding-large w3-hover-black" style="font-size: 30px; margin-bottom: 30px">Assay</button></div>
 
       <div onclick="obj=document.getElementById('menu1').style; obj.display=(obj.display=='none')?'block':'none';" style="text-align: center;" style="margin-bottom: 30px"><button class="w3-button w3-red w3-padding-large w3-hover-black" style="font-size: 30px; margin-bottom: 30px">Assay</button></div>
  
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         <a href="https://2017.igem.org/Team:TokyoTech/Experiment/AHK4_Assay">
 
         <a href="https://2017.igem.org/Team:TokyoTech/Experiment/AHK4_Assay">
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         <img src="https://static.igem.org/mediawiki/2016/4/47/Tokyo_Tech_JASSO.gif" style="width:100%">
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         <img src="https://static.igem.org/mediawiki/2017/archive/5/54/20171030133245%21T--Tokyotech--AHK4construct.png" style="width:100%">
 
         <div class="w3-container">
 
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           <h3>AHK4 Assay</h3>
 
           <h3>AHK4 Assay</h3>
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<div style="padding: 10px; margin-bottom: 10px; border: 1px dotted #333333;width: 90%;border-radius: 10px">
 
       <h4 style="text-align: center">InterLab</h4>
 
       <h4 style="text-align: center">InterLab</h4>
  
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       In iGEM 2017, the InterLab Study is one of the bronze medal criteria. We measured the GFP expression level of the six designated devices, positive control and negative control using the plate reader. It was the second time for our team to join this InterLab Study (The first time in iGEM 2015).
 
       In iGEM 2017, the InterLab Study is one of the bronze medal criteria. We measured the GFP expression level of the six designated devices, positive control and negative control using the plate reader. It was the second time for our team to join this InterLab Study (The first time in iGEM 2015).
 
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Latest revision as of 03:40, 2 November 2017

<!DOCTYPE html> Coli Sapiens

iGEM Tokyo Tech

Experiment: Overview

Introduction

For convenience of description, we devided experimental section into two parts. One is a series of assays to validate signal transmission system from bacteria to human cells. The other is a assay (AHK4 Assay) to validate the reverse system from human cells to bacteria. You can see the list of assays we conducted and also, a summary of InterLab activity.

Signal transmission system from bacteria to human cells

~ Integration of systems derived from bacteria and human cells ~

In our system, the transcription level is controlled by integrating quorum sensing (bacterial cell-to-cell communication) and NF-kB, transcription factor in mammalian cell. We used this system the signal transmission from bacteria to human cells. To validate the system, we conducted the assays shown below.

Fig. 1 Mechanism of signal transmission system from bacteria to human

TraI Assay

Chimeric
Transcription Factor
Assay

TraR Reporter
Assay

Transcriptome
Analysis

TraI Improvement
Assay


InterLab

In iGEM 2017, the InterLab Study is one of the bronze medal criteria. We measured the GFP expression level of the six designated devices, positive control and negative control using the plate reader. It was the second time for our team to join this InterLab Study (The first time in iGEM 2015).

Hajime Fujita with W3.CSS: All Rights Reserved